• 한국식품조리과학회지
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• 제29권4호
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• pp.399-406
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• 2013

The purpose of this study was to investigate eating habits, healthy eating behaviors, food preference etc. of university students for them to enjoy delightful and healthy campus life and offer preliminary data to the related educational facilities and households for them to make use of the data for making out a menu. We conducted survey aiming at 4-year-course students in Jeonbuk area and survey period was from May 6th of 2013 to May 24th of 2013. The result are as followings. First, food habit evaluation with 10 questions shows that they are inclined to have refreshments, water, vegetables, fruits, grains and protein food often and also eat food blandly. So, we consider this finding is generally positive. Second, through the research on their healthy eating behaviors, we learned their interests in healthy food is average and their selection criteria for healthy food is nutrition value. Information sources for healthy food are mainly TV or mass media and good healthy food in their opinion is Korean food. Third, the findings of food preference show university students like the white rice most but don't like the rice with beans most in terms of rice type. We also found their favorite food is fried rice, favorite noodle is spaghetti, favorite soups are thick beef soups, favorite broth is potato and pork rib broth, favorite stew is kimchi stew, favorite steamed dish is braised short ribs and favorite meat is pork.

• 박물관보존과학
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• 제9권
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• pp.95-104
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• 2008

광주 신창동 유적에서 출토된 목제 유물에 대해 수종분석 37점, 칠분석 3점등총 40점에 대하여 분석하였다. 결과 목제품에 사용된 수종은 상수리나무류, 벚나무류, 살구나무류, 오리나무류, 단풍나무류, 버드나무류, 사시나무류 등으로 총 7종이 식별되었으며, 특히 칠기와 접시 같은 생활용품의 경우에는 모두 산공재 수종을 사용하였다. 칠도막 분석 결과 유공칠기와 통형칠기 동체부 5는 칠한 횟수에서 차이가 있지만 밑칠을 칠한 후 맨 상층에 흑색안료와 칠을 혼합하여 칠하였으며, 5절판은 토분과 흑색안료를 칠과 혼합하여 밑칠을 한 후에 상층에 흑색안료를 혼합한 칠을 칠한 것으로 확인되었다.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.62-62
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• 2001

Recently, production of transgenic animal by nuclear transfer has been known as a useful method. The production of cloned offspring derived from nuclear transfer depends upon a variety of factors such as species, donor cells type and cell cycle, and source of recipient ova. Therefore, we attempted a different transgenic methods using follicular granulosa cells (GCs). In general, ovulated GCs undergoes lutenization and transformation in vitro which might defective effects on developmental potential. In order to avoid the GCs transformation in vitro culture system, we introduced a direct injection of retrovirus into the follicles and then collected them mechanically from ovaries of 6-8 week-old ICR mice. Retrovirus vector constructed with pLN $\beta$ EGFP was injected into the follicles. The follicles are cultured in $\alpha$ -MEM supplemented with human FSH, LH and ITS in Costar Transwell dish for 4 days. Survival rate of virus injected follicles was 52.1% (12/23) and expression rate of EGPP gene was 33.3% (4/12). In this study, we found GCs performed transgenesis in our culture system. In addition, the GCs in follicle may be developed in vivo like environment rather than in vitro environment. Thus, the use of GCs as donor cells may be useful in the nuclear transfer for cloning of genetic modification. Therefore, these results suggest that follicular GCs can be transfected by viral vector during folliculogenesis in vitro.

• The Korean Journal of Physiology and Pharmacology
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• 제21권3호
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• pp.335-343
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• 2017

Atopic dermatitis (AD) is an inflammatory skin condition accompanied by symptoms such as edema and hemorrhage. Kimchi is a traditional fermented Korean dish consisting of various probiotics. In this study, the therapeutic effect of Lactobacillus plantarum CJLP55 isolated from Kimchi was studied in AD-induced mice. Orally administered Lactobacillus strain, CJLP55, suppressed AD symptoms and high serum IgE levels. CJLP55 administration reduced the thickness of the epidermis, infiltration of mast cells and eosinophils into the skin lesion, enlargement of axillary lymph nodes, and increase in cell population in axillary lymph nodes. CJLP55 treatment decreased the production of type 2 cytokines, such as interleukin (IL)-4, IL-5, IL-10, IL-12, interferon (IFN)-${\gamma}$, and IL-6,which were stimulated by house dust mite extracts, in the axillary lymph node cells. Orally administered CJLP55 exhibited a therapeutic effect on house dust mite-induced AD in NC/Nga mice after onset of the disease by altering immune cell activation. The Lactobacillus strain, CJLP55, isolated from Kimchi, suppressed AD. Our results suggest its possible use as a potential candidate for management of AD.

• 천문학회지
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• 제47권6호
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• pp.293-302
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• 2014

Simultaneous time monitoring observations of $H_2O$ $6_{16}-5_{23}$, SiO J = 1-0, 2-1, 3-2, and $^{29}SiO$ ${\upsilon}=0$, J = 1-0 lines are carried out in the direction of the Mira variable star TX Cam with the Korean VLBI Network single dish radio telescopes. For the first time, the $H_2O$ maser emission from TX Cam is detected near the stellar velocity at five epochs from April 10, 2013 (${\phi}=3.13$) to June 4, 2014 (${\phi}=3.89$) including minimum optical phases. The intensities of $H_2O$ masers are very weak compared to SiO masers. The variation of peak antenna temperature ratios among SiO ${\upsilon}=1$, J = 1-0, J = 2-1, and J = 3-2 masers is investigated according to their phases. The shift of peak velocities of $H_2O$ and SiO masers with respect to the stellar velocity is also investigated according to observed optical phases. The $H_2O$ maser emission occurs around the stellar velocity during our monitoring interval. On the other hand, the peak velocities of SiO masers show a spread compared to the stellar velocity. The peak velocities of SiO J = 2-1, and J = 3-2 masers show a smaller spread with respect to the stellar velocity than those of SiO J = 1-0 masers. These simultaneous observations of multi-frequencies will provide a good constraint for maser pumping models and a good probe for investigating the stellar atmosphere and envelope according to their different excitation conditions.

• 천문학회지
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• 제48권5호
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• pp.267-275
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• 2015

Simultaneous time monitoring observations of H₂O and SiO maser lines were performed toward the D-type symbiotic binary system V407 Cyg with the Korean VLBI Network single dish radio telescope. These monitoring observations were carried out from March 2, 2010 (optical phase ϕ = 0.0), 8 days before the nova outburst on March 10, 2010 to June 5, 2014 (ϕ = 2.13). Eight days before the nova outburst, we detected the SiO v = 1, 2, J = 1–0 maser lines which exhibited values of 0.51 K (∼ 6.70 Jy) and 0.71 K (∼ 9.30 Jy), respectively, while after the outburst we could not detect them on April 2 (ϕ = 0.04), May 5 (ϕ = 0.09), May 8 (ϕ = 0.09), or on June 5, 2010 (ϕ = 0.13) within the upper limits of our KVN observations. After restarting our monitoring observations, we detected SiO v = 2, J = 1–0 masers starting on October 20, 2011 (ϕ = 0.83) and detected SiO v = 1, J = 1–0 masers starting on December 22, 2011 (ϕ = 0.92). These results provide clear evidence of the interaction between the shock from the nova outburst and the SiO maser regions of the Mira envelope. The peak emission of SiO v = 1, 2, J = 1–0 masers always occurred at blueshifted velocities with respect to the stellar velocity except for that of SiO v = 1 at one epoch. These phenomena may be related to the redistribution of SiO maser regions after the outburst. The peak velocity variations of SiO masers associated with stellar pulsation phases show an increasing blueshifted trend during our monitoring interval after the outburst.

• 한국지역사회생활과학회지
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• 제14권2호
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• pp.49-62
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• 2003

Breakfast consumption is important for nutritional balance in all population groups. The objective of this study is to seek healthy Korean with continuous traditional breakfast consumption of rice. One thousand participants who go to work or school every morning were selected by stratified method and interviewed by regional home extension workers in 24 cities, Kyeonggi province, Korea. The questions included breakfast eating habits, perceived health status,and food preferences for breakfast. The students (70.5%) were composed with elementary (17.2%), middle school(15.7%), high school(16.5%), and university(21.l%)students. The rest 29.5% were workers. The recognized importance of eating breakfast was negatively correlated with the perceived health status. It means that lowering health status made them recognize the importance of breakfast. Workers had more boiled rice with side dishes for breakfast(58.6%) than students (40.2%). The reasons of selected items for breakfast were habit (28.5%), and health (14.6%). The propensity of convenience and preference was more frequent in male workers than male students. For breakfast, ready-to-eat food (rice-roll or rice ball, retort rice, and fast-food) preferred more in middle & high school students, but boiled rice with side dishes was preferred more in workers. Boiled rice with divers cereals was selected for main dish in workers, however boiled rice only in students if obliged. We suppose the problem in next decade would be skipped breakfast in regular attendance position. For breakfast with ready-to-eat rice or something to eat in short time, students and workers should be educated to those who are too busy to eat breakfast for the enhancement of human resources.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제32권6호
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• pp.495-505
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• 2006

In this study we evaluate the effects of bFGF-BB and PDGF on in vitro proliferation, differentiation and mineralization of mesenchymal stem cells (MSCs) from rat. MSCs were prepared from the bone marrow of 6 or 7-week-old male rats with a technique previously described by Maniatopoulos et al. in 1988. Lineage differentiation to osteogenesis, chondrogenesis and adipogenesis were performed. At first, we characterized the cultured cell on passage 1, 3, 5, 7 with immunocytochemical staining using CD29, 44, 34, 45, ${\alpha}$-SMA and type I collagen. And to study the effects of bFGF and PDGF-BB on proliferation, differentiation and mineralization, we seeded the expanded cell at a density of 6 $6{\times}10^3\;cells/cm^2$ to 100-mm dish for evaluation of cell proliferation and MTT assay was carried out on day 2, 4, 7, 9. We also resuspended the cells with same density $(6{\times}10^3\;cells/cm^2)$ to 24 well plates for subculture. On the following day, the attached cells were exposed to 2.5ng/ml bFGF and/or 25ng/ml PDGF-BB daily during 5 days. The osteocalcin (OC) level was assessed and mineral contents were evaluated with alizarin red S staining on subculture day 2, 7, 14, 21. We identified the mesenchymal stem cell from the bone marrow derived cells of rat through their successful multi-differentiation and stable display of its phenotype. And bFGF and PDGF-BB showed the effect that inhibited osteoblastic differentiation and mineralization mildly in above concentration at in vitro culture. This study was supported by grant 04-2004-0120 from the Seoul National University Hospital Research Fund.

• Korean Chemical Engineering Research
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• 제51권6호
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• pp.760-765
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• 2013

액적기반 미세유체 시스템을 이용해 난백단백질인 라이소자임의 결정화실험을 하였다. Flow-focusing 칩을 이용해 water-in-oil 형태의 액적을 만들고 페트리 디쉬와 십자몰드에 넣은 후, 액적 내부에서 라이소자임 수용액과 침전제 (NaCl) 사이의 액-액 반응을 관찰하였다. 그리고 수용액의 pH가 4.8일 때와 7.2일 때의 결정형태를 비교하였다. 그 결과, pH 4.8에서는 다면체 또는 판상형의 결정이 형성되었고, pH 7.2에서는 침상형 결정이 생성되었다. pH 4.8, 7.2 두 경우 액적이 홀로 있을 때에는 액적부피가 유지되거나 감소하면서 결정이 형성되었다. 하지만 액적이 서로 인접해 있을 때는 액적사이의 상호작용이 관찰되었고, 두 pH에서 다른 경향성을 보였다. pH 4.8에서는 인접한 액적의 부피에 영향을 주어 한 액적의 부피가 커졌고, 부피가 커진 액적에서 결정이 형성되었다. pH 7.2에서는 부피에 영향을 서로 주지 않고 각각의 액적에서 결정이 형성되었다.

• 한국지역사회생활과학회지
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• 제14권3호
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• pp.75-87
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• 2003

This study was conducted to find the number of meals skipped and the types of main meals consumed by people in Miryang and Daegu. The subjects consisted of elementary school students(ES), middle school students(MS), high school students(HS), college students(CS) and adults, divided into below the age of 40(AU) and above the age of 40(AA). Data were collected through a 7-day dietary record from 941 subjects. The definition of rice-based meal(RM) and wheat-based meal(WM) depends on the type of grain eaten in the main dish, rice or wheat flour, respectively, regardless of the menu. Out of all the meals consumed by the subjects during 7 days before the survey, 71.5% of the meals consumed were RM, 13.1 ％ of the meals consumed was WM and 12.5% of the meals was skipped. Of the meals skipped, breakfast-skipping was the highest with 27.1%. There's no difference by region and sex. But there were several differences by age. The rates of skipped meal were high for CS, AU, HS, and MS in order. Especially the breakfast-skipping for CS was highest as 47.9%. In the types of main meal, the rates of RM was highest at breakfast and lowest at lunch. The rates of RM was higher in Miryang than Daegu, and there was no difference by sex. The rates of RM was high for AA, HS, ES and AU in order. ES and HS, who had highest rates of WM, had the highest WM consumption at lunch. It is because of school food service at lunch. Since the younger population consumed more RM compared to the older population, it can be speculated that more WM will be consumed in the future. Also efforts need to be made to discourage skipping meals.