• Parasites, Hosts and Diseases
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• v.29 no.2
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• pp.113-120
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• 1991

When the component proteins in crude saline extract of 13-week old adult Paragonimus westermani were observed by non-denaturing discontinuous-polyacrylamide gel electrophoresis (Disc-PAGE), 8 distinct bands were clearly recognized. Molecular weight (MW) of each band protein, numbered in sequence from cathodal side which appeared in 10% separating gel, was measured first by Ferguson plot utilizing different gel concentrations from 10% to 4.5%. MW of band 1 Protein (known as egg Protein) was 440 kDa. And MW of other band Proteins were: 386 kDa in band 2, 17.4 kDa in band 3, 17kDa in band 4, 14.3 kDa in band 5, 46 kDa in band 6, 38 kDa in band 7 and 23 kDa in band 8. When the proteins in the crude extract were separated into fractions by molecular sieve chromatography through 1.6 (Φ)×70cm sired Sephacryl 5-300 Superane column and revisualized by Disc-PAGE in 8% gel, the sequence of fluted proteins was band 1, band 2, band 6, band 7 and bands 3,4,5 and 8. This elusion profile confirmed MW of each band protein in the crude extract as measured by Ferguson plot.

• Parasites, Hosts and Diseases
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• v.24 no.1
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• pp.49-54
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• 1986

To evaluate the immature eggs of Paragonimus westermani as a source of diagnostic antigen, about a million eggs which were excreted by 104 adult worms were collected; their saline extract(soluble egg antigen; PwSEA) was prepared. The specific IgG and IgM antibody levels were observed in experimental dog paragonimiasis by micro ESISA, using PwSEA as well as whole worm extract of 12 week-old P. westermani(PwWWE). The protein composition of the PwSEA was observed by disc-PAGE. The results could be summarized as follows: 1. Specific IgG antibody to PwSEA begant to increase on 8 weeks after the experimental infection; it maintained its high level until the observation period of 13 weeks. The levels of IgM antibody to PwSEA however, did not show any significant change. 2. Specific IgG antibody to PwWWE began to increase earlier from 2 weeks after the infection and continued to increase until the observation period of 13 weeks. Its level was much higher than that to PwSEA. Specific IgM antibody to PwWWE increased temporarily during 2-8 weeks after the infection. 3. By disc-PAGE, PwSEA showed 2 protein bands of very low motility. The bands of PwSEA corresponded to the frist and second bands in the electrophoretic pattern of PwWWE of the 12 week-old worms. The above results indicated that the PwSEA induced antibody production in dog paragonimiasis, but its antigenicity was weaker than PwWWE to be used as a diagnostic antigen.

• Parasites, Hosts and Diseases
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• v.30 no.2
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• pp.91-100
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• 1992

Tissue origin of individual component proteins in crude extract of adult Paragonimus westermani was investigated. Major soluble component proteins were separated by disc-PAGE in 8% slab gel. By predefined Rf values, strips of gel containing each band protein was cut out. Each band protein was eluted by electrophoresis. Monospecific antibodies were prepared by immunizing rabbits with each band protein. When peroxidase-antiperoxidase (PAP) staining was done, antiserum to Band 1 reacted to content of eggs both in the worm and in the infected lung tissue. Antiserum to Band 2 reacted to parenchymal tissue of the worm. Antiserum to Band 4 showed the positive reaction at intestinal content while that to Band 5 reacted to the intestinal epithelial border. Antiserum to combined proteins of Bands 617 and that to Band 8 reacted to parenchymal tissue of the worm respectively. From the results, the origin of individual proteins in crude extract of adult P. westermani could be differentiated.

• Transactions of the Society of Information Storage Systems
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• v.8 no.2
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• pp.67-71
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• 2012

Page-oriented holographic data storage (HDS) is very sensitive to the tilt error. Therefore, tilt error should be measured and compensated. Especially, mechanical tilt measurement method cannot cope with tilt error measurement because photopolymer medium has shrinkage problem. Therefore, the method to solve this problem is using the reconstructed image which can represent both tilt and shrinkage effect. In this paper, we suggest disc tilt measurement algorithm using image pattern of retrieval data.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2007.11a
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• pp.1333-1334
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• 2007

Page-oriented angle multiplexing Holographic data storage system is the one of promising techniques for high capacity and data transfer rate, but its size and narrow tilt margin is pointed as a demerit. To overcome this weak point, in this paper, 2-axis deck mechanism for compensation of disc is employed to Compact size Holographic Data Storage (CHDS) which consists of minimized optical components and small 2-axis actuator moving linear & angle direction. Compensation of radial/tangential disc tilt with 2-axis deck mechanism is demonstrated.

• The Korean Journal of Food And Nutrition
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• v.7 no.1
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• pp.23-28
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• 1994

Soybean $\beta$-amylase was purified by DEAE-cellulose ion exchange chromatography, Sephadex G-100 gel chromatography, CM Sephadex C-50 ion exchange chromatography and CM Sephadex C-50 ion exchange rechromatography The purified enzyme showed 1, 020 unit/mg of specific activity. The purified enzyme was identified as homogenious by disc PAGE and analysis of reaction product.

• Journal of Digital Contents Society
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• v.14 no.1
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• pp.15-23
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• 2013

In response to the demands of large-scale data processing with low-power and new application, a storage system using SSD (Solid State Disk/Drive) with fast input-output performance instead of hard disc has appeared as storage device. Studies on methods to overcome specific problems of SSD such as various processing data units, out-place-update and limited delete count have been actively conducted. However, declining performance and stability have not been resolved yet when storing case specific data with small scale that causes frequent random write in hard disc or SSD. This thesis suggests a system structure that stores index requesting frequent random write in NVRAM capable of byte access by using characteristics such as byte unit fast read / write of NVRAM, non-volatile and smaller size of actual changed data size in index page than block size.

• Transactions of the Society of Information Storage Systems
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• v.3 no.1
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• pp.23-27
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• 2007

Blu-ray disc 이후의 차세대 고밀도 광 기록 기술로 주목받는 홀로그래픽 데이터 스토리지 시스템을 설계, 제작하고 기록 재생 test 를 수행하였다. 시스템은 각도 다중화법을 채택하였고, 파장 405nm, NA 0.55 신호광 Fourier 변환 렌즈로 미디어에 defocus 하여 기록하였다. 1 page 용량은 raw bit 로 0.74Mbit 이고, SLM 과 CCD 의 over-sampling 비율은 1:1.5 가 되도록 구성하였다. 얻어진 raw 이미지는 위치 및 distortion 보상등의 신호처리 후, 70page 다중화 조건에서 bER $1{\times}10-2$ 수준으로 재생해 낼 수 있었다. 온도 변화에 의한 신호 열화를 보상하기 위해 External Cavity Tunable Blue LD 를 개발하여 온도 변화 $10^{\circ}C$까지 보상 가능함을 확인하였다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.21 no.6
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• pp.725-730
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• 1992

The internal invertase was purified from cell free extract of Rhodosporidium toruloides IFO 0559-M-919 by acid precipitation, ion-exchange chromatography and gel filtration to the unique enzyme protein on disc electrophoresis. We have found out that molecular weight of purified internal invertase was 90,000 by gel filtration and the purified enzyme was protein with 4 homogeneous subunits appearing as single band of 22,000daltons on SDS-polyacrylamide gel electrophoresis.

• Transactions of the Society of Information Storage Systems
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• v.9 no.1
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• pp.5-9
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• 2013

Page-oriented holographic data storage (HDS) is very sensitive to the disturbances. However, vibration effect by disc imbalance can be ignored because data pages are recorded and retrieved with stop-go rotation. Therefore, just estimating de-track due to eccentricity of disc is enough to construct stable track servo system. In this paper, propose the spacing of track servo patterns optimization method using Least Mean Square (LMS) estimation algorithm. Through the patterns spacing optimization, storage density maximize can be achieved.