• Journal of Physiology & Pathology in Korean Medicine
• /
• v.19 no.4
• /
• pp.938-944
• /
• 2005

Down-regulation of melanin synthesis is required for recovery of pigmentary disorders and it is known that direct inhibitors of tyrosinase suppress melanin synthesis. We screened several oriental medicinal plants using B16/Fl0 cells and found that the methanolic extract of Cnidii Rhizoma down-regulated melanin synthesis effectively. Although the proliferation of Bl6/Fl0 cells was decresed by the methanolic extract of Cnidii Rhizoma, it did not appear necrosis. Bl6/F10 cells incubated with the methanolic extract of Cnidii Rhizoma showed reduced pigmentation and tyrosinase activity. Western blotting revealed that the amount of tyrosinase was decreased by the methanolic extract of Cnidii Rhizoma. These results suggest that the inhibitary effect of the methanolic extract of Cnidii Rhizoma on melanogenesis is due to the suppression of tyrosinase in Bl6/F10 cells and Cnidii Rhizoma is a candidate for an efficient whitening agent.

• Journal of Soil and Groundwater Environment
• /
• v.15 no.6
• /
• pp.1-8
• /
• 2010

The groundwater level distribution and characteristics of responses to rainfall were examined in the Haean basin of Yangu that has a single stream exit to the east. The groundwater levels showed a circular or elliptical distribution converging the center of the basin with different hydraulic gradients in the north and south regions. The waterlevel elevations exhibited a perfect correlation with topographic elevation ($r^2=0.99$) while the depth to water showed a rather weak correlation ($r^2=0.49$). The water table fluctuation (WTF) method yielded recharge ratios of 6.1~12.65% ($S_y=0.02$) and 15.2~28.5% ($S_y=0.05$). The waterlevels of HG3 well, which is much proximal to a stream, were weakly auto-correlated but they were highly sensitive to direct infiltration from the rainfall event. The shorter regulation times of the HG3 represented a quicker dissipation of the input stress (rainfall).

• Molecules and Cells
• /
• v.26 no.3
• /
• pp.265-269
• /
• 2008

Calumenin, a multiple EF-hand $Ca^{2+}$ binding protein is located in the SR of mammalian heart, but the functional role of the protein in the heart is unknown. In the present study, an adenovirus gene transfer system was employed for neonatal rat heart to examine the effects of calumenin over-expression (Calu-OE) on $Ca^{2+}$ transients. Calu-OE (8 folds) did not alter the expression levels of DHPR, RyR2, NCX, SERCA2, CSQ and PLN. However, Calu-OE affected several parameters of $Ca^{2+}$ transients. Among them, prolongation of time to 50% baseline ($T_{50}$) was the most outstanding change in electrically-evoked $Ca^{2+}$ transients. The higher $T_{50}$ was due to an inhibition of SERCA2-mediated $Ca^{2+}$ uptake into SR, as tested by oxalate-supported $Ca^{2+}$ uptake. Furthermore, co-IP study showed a direct interaction between calumenin and SERCA2. Taken together, calumenin in the cardiac SR may play an important role in the regulation of $Ca^{2+}$ uptake during the EC coupling process.

• Journal of Korean society of Dental Hygiene
• /
• v.16 no.1
• /
• pp.53-59
• /
• 2016

Objectives: The purposes of this study is to examine the relationship between emotional labor and burnout among dental hygienists. Methods: A self-reported questionnaire was completed by 247 dental hygienists from September 4 to October 9, 2014. The questionnaire consisted of emotional labor, job burnout, and job stress. Emotional labor included frequency of emotional expression, compliance with emotional regulation, emotional diversity, and emotional dissonance by the instrument pf Morris and Feldman. Job burnout was adopted from Maslach burnout inventory(MBI). Job stress was adpoted from instrument of Chang. Except 13 incomplete answers, 234 data were analyzed by AMOS 18.0 and SPSS 18.0 program. Results: The emotional labor has a significant positive relation to the job burnout. There was a statistical significance between emotional labor and job stress. Job stress had a positive relation to job burnout. Job stress had a mediating effect between emotional labor and job burnout. Emotional labor had a direct effect on the job burnout. Job stress also had a mediating effect on job burnout. Conclusions: It is necessary to improve working environment that can enhance emotional balance and to develop job stress management program.

• Journal of Korean Society for Atmospheric Environment
• /
• v.16 no.1
• /
• pp.79-87
• /
• 2000

This study analyzed the regulations concerning odor management or control in Korea, Japan, and developed countries such as USA and Germany. The Korean restriction standards and measurement methods for offensive odor substances are very similar to the Japanese standards and methods promulgated in 1976. Japan promulgated the odor control act and the specific measurement methods of the offensive odor substances in 1973. In Japan, currently, the local governments can establish their own odor restriction areas and proclaim their own standards suitable for their situations. Also, Japan has more strict regulations and more offensive odor substances to be monitored than Korea. The restriction standards of odor in German is quite similar to the permissible limits and standards of air pollutants at emission sources in Korea. Many states in USA are adopting their own restriction standards and measurement methods, e.q., the ambient air pollution standards, organic functionstest methods by dilation, and establishment and addition of air pollution control equipment at emission sources. Finally, this study analyzed the problems of the regulations, standards, and measurement methods concerning the Korean offensive odor management. Since the direct organic fuctions measurement(DOFMM) method is depending upon the personal sensitives of odor substances, the measurement odor value can be equivocal. That is, the odor measurement value by an examines can differ from that by the other examines. Also, there can be conflicts with the measured odor values depending upon the applied measurement methods, e.q., the DOFMM, the dilation method, and the instrumental analysis method.

• Proceedings of the Botanical Society of Korea Conference
• /
• 1996.07a
• /
• pp.26-38
• /
• 1996

To investigate the regulation of plant histone H2A gene expression, we isolated two H2A genes (TH254 and TH274) from wheat, which encode different types of variants. Both genes had an intron in the coding region. In the promoters, some characteristics sequences, such as Oct and Nona motifs, which are conserved among plant histone genes were also found, and they were located in a short region (about 120 bp) upstream from the putative TATA box. Analyses of promoter activity with H2A-GUS fusion genes in the transient system using tobacco protoplasts revealed novel types of positive cis-acting sequences in the TH254 promoter: a direct repeat of a 13-bp sequence (AGTTACATTATTG) and a stretch composed of an AT-rich sequence (ATATAGAAAATTAAAA) and a G-box (CACGTG). A quantitative S1 assay of the mRNA amounts from the TH254/GUS and TH274/GUG chimeric genes in stably transformed and cell cycle-synchronized tobacco cell lines showed that the promoters of both genes contained at least one cis-acting element responsible for S phase-specific expression. Histochemical analysis of transgenic tobacco plants carrying the chimeric genes showed that the promoters of the two H2A genes were both active in developing seedlings and flower organs but regulated in different manner.

• Journal of the Korea Concrete Institute
• /
• v.11 no.4
• /
• pp.43-54
• /
• 1999

Shear tests are performed on four full-scale 12.5 m proto-type models, "least depth double tee," which are resulted from the optimization process. Domestic superimposed live load regulation, domestic material properties which is available to product. Korean building code requirements, construction environments and economy are considered as the main factors to establish the process. All of the specimens tested fully comply with the shear strength requirements as specified by ACI 318-95. The research has shown following results. 1) The development length requirement of ACI 318-95 does not seem a good predictor for the estimation of bond failure in a beam with the strands below the supports. 2) The load required for the first initial coner cracking in the dap end and first web shear cracking does not seem to have any relation with the dimension and shear strength of the section in the test beams. 3) The strand slip has a direct relationship with the web shear cracking. However, the coner cracking in the dap end does not give any help for the slip in anchorage. 4) Use of whole area for bearing steel at the bottom of dap end is desired for safe bearing pressure design in the precast prestressed double tee beams. 5) The deflection of beam influences directly on the amount of strand slip at the anchorage after initiation of it, and relationship between them are very linear.

• KIEAE Journal
• /
• v.12 no.6
• /
• pp.29-38
• /
• 2012

The current building energy efficiency rating certification regulation on apartment houses evaluates only the saving rate of energy consumed during operation, i.e. use, of a building, but doesn't consider the energy consumptions and environmental load($CO_2$) emissions occurring during the whole lifetime of a building. So this study calculated the energy consumptions and environmental load emissions occurring during the whole lifetime of a building, selected reference schemes and alternative items by design variables to present a design draft considering energy efficiency and environmental performance, and evaluated the total cost by combining and calculating its direct cost(material and heating cost) and indirect cost(environmental cost), for an existing apartment house as an evaluation object. As a result of analysis, the change of heating cost by alternative items of design variables showed a 4~8% change rate compared to the reference scheme, and the material cost of design variable 7 showed a maximum 4.4 times change rate in the alternative plan 6 compared to the reference scheme. The environmental cost showed a similar change rate to the material cost change rate in general, but showed a similar environmental cost change rate to the heating cost change rate in case of design variables 4-1, 4-2, 7.

• Proceedings of the KIEE Conference
• /
• 2005.04a
• /
• pp.134-139
• /
• 2005

This paper presents a cost effective quasi-resonant soft-switching PWM high frequency inverter with minimum circuit components. This inverter can achieve wider soft commutation, simpler power circuit configuration, smaller volumetric size, lower cost and wider power regulation range, higher-efficiency as compared with single ended quasi-resonant ZVS-PFM inverter and active voltage clamped quasi-resonant ZVS-PWM inverter. The operation principle of the proposed inverter is described on the basis of the simulation and experimental results, together with its operating performances in steady state. The operating performances of this unique proposed high frequency inverter based on ZVS and ZCS arms-related soft commutation principle is evaluated and discussed as compared with the active voltage-clamped ZVS-PWM inverter and a conventional single-ended ZVS-PFM inverter. The practical effectiveness of a novel type quasi-resonant soft-switching PWM high frequency inverter using IGBT is actually proved for consumer induction heated appliances as rice cooker, hot water producer, steamer and super heated steamer. The extended bidirectional circuit topology of quasi-resonant PWM high frequency inverter with minimum circuit components is demonstrated, which operate as the direct frequency changer.

• Biomedical Science Letters
• /
• v.15 no.1
• /
• pp.61-66
• /
• 2009

Leflunomide is an immunomodulatory agent used for the treatment of rheumatoid arthritis (RA). Leflunomide known as a regulator of iNOS synthesis which largely decreases NO production in diverse cell type. However, the effect of leflunomide on chondrocyte is still poorly understood. In our previous studies, we have shown that direct production of Nitric oxide (NO) by treating chondrocytes with NO donor, sodium nitroprusside (SNP), causes apoptosis via p38 mitogen-activated protein kinase in association with elevation of p53 protein level, caspase-3 activation. In this study, we characterized the molecular mechanism by which A77 1726 inhibit apoptosis. We found that A77 1726 inhibit NO-induced apoptosis as determined by MTT (Thiazolyl Blue Tetrazolium Bromide) assay and DNA fragmentation. The inhibition of apoptosis by A77 1726 was accompanied by increased PI-3 kinase and AKT activities. So, inhibition of phosphatidylinositol (PI)-3kinase with LY294002 rescued apoptosis. Triciribine, the specific inhibitor of AKT, also abolished anti-apoptotic effect. Our results indicate that A77 1726, the active metabolite of leflunomide, mediates NO-induced apoptosis in chondrocytes by modulating up-regulation of PI-3 kinase and AKT.