• BMB Reports
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• v.29 no.3
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• pp.221-224
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• 1996

To examine the growth-phase dependent control of Escherichia coli rnpB gene we used a combination of Northern analysis for RNA determination and Southern analysis for plasmid DNA determination. The relative amounts of metabolically unstable transcript derived from the internally deleted rnpB gene harbored on a multicopy plasmid as well as the relative plasmid contents were measured by Northern analysis and Southern analysis, respectively, of total nucleic acids from E coli cells containing the plasmid. The relative transcription activity of the rnB was represented by a ratio of the relative amount of the transcript to that of the plasmid DNA during bacterial growth. The rnpB transcription increased rapidly with time during exponential growth, but started to decrease before the transition period of an exponential growing cell culture into the stationary phase. Although the expression pattern was similar to the changes of ${\beta}-galactosidase$ activity expressed from the lysogenic strain carrying the chromosomal rnpB-lacZ fusion which were shown in a previous work, the present data appears to represent a more actual growth-phase control of the rnpB transcription than the previous data by the ${\beta}-galactosidase$ assay. In addition the present method described for a direct analysis of both RNA and plasmid DNA provides a rapid and efficient method that can applied to an examination of transcription control by using a multicopy plasmid.

• Analytical Science and Technology
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• v.33 no.1
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• pp.42-48
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• 2020

Bromate is a disinfection by-product generated mainly from the oxidation of bromide during the ozonation and disinfection process in order to remove pathogenic microorganism of drinking water, and classified as a possible human carcinogen by International Agency for Research of Cancer (IARC) and World Health Organization (WHO). For the purpose of determining the trace level concentration of bromate, several sensitive techniques are applied mostly based on suppressed conductivity detection and UV/Visible detection after postcolumn reaction (PCR). In this study, the suppressed conductivity detection method and the PCR-UV/Visible detection method through the triiodide reaction were compared to analyze the trace bromate in water samples and estimated for the availability of these analytical methods. In addtion, the state-of-the-art techniques was applied for the determination of trace level bromate in various water matrices, i.e., soft drinking water, hard drinking water, mineral water, swimming pool water, and raw water. In comparison of two analytical methods, it was found that the conductivity detection had the suitable advantage to simultaneously analyze bromate and inorganic anions, however, the bromate might not be precisely quantified due to the matrix effect especially by chloride ion. On the other hand, the trace bromate was analyzed effectively by the method of PCR-UV/Visible detection through triiodide reaction to satisfactorily minimize the matrix interference of chloride ion in various water samples, showing the good linearity and reproducibility. Furthermore, the method detection limit (MDL) and recovery were 0.161 ㎍/L and 101.0-108.1 %, respectively, with a better availability compared to conductivity detection.

• Journal of Food Hygiene and Safety
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• v.16 no.1
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• pp.61-65
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• 2001

To investigate the optimum condition of 3-monochloro-1, 2-propanediol(MCPD) analysis, gas chromatography with electron capture detector was used. Determination of MCPD derivatized with phenylboric acid was more effective than that of underivatized MCPD. In derivatization of MCPD with phenyl boric acid, there were no significantly different between boiling for 2min at 9$0^{\circ}C$ and vortexing for 5min at room temperature. Extrelut column was suitable for extraction of MCPD diluted in 20% NaCl solution and recovery rates were higher than direct extraction of MCPD with ethyl acetate. But, the method of direct extraction of MCPD with ethyl acetate was useful for rapid ants qualitative analysis. The sample extracted in soysauce(ganjang) was derivatized with phenylboric acid and analyzed by gas chromatography-mass selective detector. That was confirmed as MCPD-phenylboronate.

• Mass Spectrometry Letters
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• v.5 no.4
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• pp.104-109
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• 2014

Nonmedical use of prescription stimulants such as methylphenidate (MPH) and amphetamine (AP) by normal persons has been increased to improve cognitive functions. Due to high potential for their abuse, reliable analytical methods were required to detect these prescription stimulants in biological samples. A direct injection liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and implemented for simultaneous determination of MPH, AP and their metabolites ritalinic acid (RA) and 4-hydroxyamphetamine (HAP) in human urine. Urine sample was centrifuged and the upper layer ($100{\mu}L$) was mixed with $800{\mu}L$ of distilled water and $100{\mu}L$ of internal standards ($0.2{\mu}g/mL$ in methanol). The mixture was then directly injected into the LC-MS/MS system. The mobile phase was composed of 0.2% formic acid in distilled water (A) and acetonitrile (B). Chromatographic separation was performed by using a Capcell Pak MG-II C18 ($150mm{\times}2.0mm$ i.d., $5{\mu}m$, Shiseido) column and all analytes were eluted within 5 min. Linear least-squares regression with a 1/x weighting factor was used to generate a calibration curve and the assay was linear from 20 to 1500 ng/mL (HAP), 40-3000 ng/mL (AP and RA) and 2-150 ng/mL (MPH). The intra- and inter-day precisions were within 16.4%. The intra- and inter-day accuracies ranged from -15.6% to 10.8%. The limits of detection for all the analytes were less than 4.7 ng/mL. The suitability of the method was examined by analyzing urine samples from drug abusers.

• Journal of the Korean Chemical Society
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• v.39 no.4
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• pp.252-256
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• 1995

A study was carried out for the development of direct determination of antimony in high purity copper metal without preseparation. The large excess of copper seemed to be performed as the matrix modifier to enhance the absorbance of antimony in copper metal. Sensitivity enhancement of Sb was obtained at the condition of the ashing temperature of $600^{\circ}C(20s){\sim}700^{\circ}C(10s)$ rather than $1000^{\circ}C(20s){\sim}1100^{\circ}C(10s)$, and the atomization temperature of $2200^{\circ}C.$ The operating pressure of argon gas was readjusted to the optimum condition at 2.0 kg/$cm^2.$ The linearity of the Sb calibration graph could be obtained in the range of 20 ppb up to 200 ppb Sb in existence of 5,000 ppm Cu with the coefficient of variation of about 5%.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.23 no.3
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• pp.323-330
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• 2005

More than ten years after the era of GPS-Photogrammetry which could provide us only three projection center of all six exterior orientation parameters, direct georeferencing with GPS/INS is now becoming a standard method for image orientation. Its main advantage is to skip or reduce the indirect ground control process. This paper describes the experimental test results of integrated sensor orientation with a commercial GPS/IMU system to approve its performance in determination of exterior orientation. For this purpose two different imaging blocks were planned and the area was photographed at a large photo scale of 1:5,000 and a medium photo scale of 1:20,000. From these data set a variety of meaningful results was acquired, i.e., the accuracy. potential of exterior orientation from direct georeferencing and combined block adjustment using these data considering different photo scales and conditions.

• International Commerce and Information Review
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• v.11 no.2
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• pp.343-367
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• 2009

This research is about global investment for managing the important position, what Korea is doing in World's main market. Considering there are some differences between developed countries' model and developing countries' model in doing direct overseas investment, they target to get political agreement and develop the new invest plan and strategy by understanding changes of Korean manufacturing companies in direct overseas investment between 2000 and 2007 and analyzing the change of yearly investment motivation factors and determining factors for investment. The result from this result let us know that company should develop their own idea for their competitive advantage by doing direct overseas investment with the existing theory which convinces the need of competitive advantage for investing overseas. I set actual model and analyze results from it with the considering that it is so important to get knowledge and information for globalizing companies to invest overseas and companies, which want to be world leading ones for their field through innovation and changes, need to have more active strategy. And, the overseas investment, which was already done in other countries, 1. Review its realities and tendency in terms of investing countries, investing industries, and its scale. 2. Set up an actual model, based on strategic combination of investing location select and determination of Korean manufacturing companies and yearly investing factor-effect analysis. 3. Analyze how the situational factors have influenced and what factors would be considered for direct overseas investment. From the analyzing result, even though it is fairly true that raising wage and getting resources, avoiding customs, and developing alternating industries for export had influenced at the beginning, overseas investing companies' policy will be influenced by the results from studying marketing-pursuit type, which emphasizes to manage trade income and outgo, keeping the balance in the black, ensuring raw materials, local producing and manufacturing by using low-wage people for local sale, and situation for changing investing tendency as service industry.

• Journal of Korean Academy of Nursing
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• v.47 no.4
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• pp.514-525
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• 2017

Purpose: The purpose of this study was to construct and test a structural equation model for Diabetes self-management (DSM) behavior and Quality of life (QoL) in older adults with diabetes who use Citizen Health Promotion Centers. The theory used this study was a combination of the Information-Motivation-Behavioral Model (IMB) and Self-Determination Theory (SDT) to reflect autonomous characteristics of participants. Methods: Data were collected from April 20 to August 31, 2015 using a self-report questionnaire. The sample was 205 patients with type 2 Diabetes who regularly visited a Citizen Health Promotion Center. SPSS 22.0 and AMOS 22.0 programs were used to analyze the efficiency of the hypothesized model and calculate the direct and indirect effects of factor affecting the participants' DSM behavior and QoL. Results: The supported hypotheses were as follows; 1) The variable that had a direct effect on QoL was health behavior adherence (${\gamma}=.55$, p=.007). 2) The variables that had a direct effect on DSM behavior were DSM information (${\gamma}=.15$, p=.023), DSM confidence (${\gamma}=.25$, p<.001), and autonomous motivation (${\gamma}=.13$, p=.048). 3) The variable that had a direct effect on DSM confidence was autonomy support (${\gamma}=.33$, p<.001). Conclusion: The major findings of this study are that supporting patient's autonomous motivation is an influential predictor for adherence to DSM behavior, and integrative intervention strategies which include knowledge, experience and psychosocial support are essential for older adults with diabetes to continue DSM behavior and improve QoL.

• Korean Journal of Agricultural and Forest Meteorology
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• v.23 no.2
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• pp.134-140
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• 2021

Global solar radiation consists of direct and diffuse radiations. Both components are necessary for not only atmospheric science and solar energy domains but also agricultural applications. In this study, the data of direct and diffuse radiations are uploaded to Github. It was observed in Naju during May 2019 to November 2020. Using this data, the previous empirical equations using the relation between clearness index and diffuse ratio were validated. All coefficients of determination (R²) and RMSE were similar as 0.79~0.80 and 0.13~0.15. However, to get the lower RMSE, other non-linear approaches will be required with more observation data.

• Geomechanics and Engineering
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• v.36 no.5
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• pp.427-440
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• 2024

This study concentrates on the 301 comprehensive caving working face, notable for its considerable mining height. The roof model is established by integrating prior geological data and the latest borehole rock stratum's physical and mechanical parameters. This comprehensive approach enables the determination of lithology, thickness, and mechanical properties of the roof within 50 m of the primary mining coal seam. Utilizing the transfer rock beam theory and incorporating mining pressure monitoring data, the study delves into the geometric parameters of the direct roof, basic roof movement, and roof pressure during the initial mining process of the 301 comprehensive caving working face. The direct roof of the mining working face is stratified into upper and lower sections. The lower direct roof consists of 6.0 m thick coarse sandstone, while the upper direct roof comprises 9.2 m coarse sandstone, 2.6 m sandy mudstone, and 2.8 m medium sandstone. The basic roof stratum, totaling 22.1 m in thickness, includes layers such as silty sand, medium sandstone, sandy mudstone, and coal. The first pressure step of the basic roof is 61.6 m, with theoretical research indicating a maximum roof pressure of 1.62 MPa during periodic pressure. Extensive simulations and analyses of roof subsidence and advanced abutment pressure under varying working face lengths. Optimal roof control effect is observed when the mining face length falls within the range of 140 m-155 m. This study holds significance as it optimizes the working face length in thick coal seams, enhancing safety and efficiency in coal mining operations.