• Natural Product Sciences
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• v.11 no.2
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• pp.67-74
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• 2005

The ethanolic extracts of the leaves and bulbs of Allium victorialis var. platyphyllum (Liliaceae) collected from Daegwallyoung (D) and Ullung Island (U) in Korea were obtained using three different extracting methods. The first extracts, DL-1 DB-1, UL-1 and UB-1, were obtained from leaves (L) and bulbs (B) dried at $90^{\circ}C$, respectively, and the second extracts, DL-2, DB-2, UL-2 and UB-2, were obtained by extracting the leaves and bulbs of fresh plant parts. The third extracts DL-3, DB-3, UL-3 and UB-3 were obtained by incubating leaves and bulbs at $36^{\circ}C$. The six extracts obtained from A. victorialis var. platyphyllum at Daegwanllyoung (cultivated site) were orally administered to examine for a possible antihepatotoxic effect in $CCl_4-induced$ rats. DL-1 exhibited the most pronounced effect. The extracts inhibited serum ALT, AST, SDH, ${\gamma}-GT$, ALP and LDH activities elevated by $CCl_4$ injection and attenuated decreased glutathione S-transferase, glutatione reductase and ${\gamma}-glutamylcysteine$ synthetase activities and a decreased hepatic glutathione. However, the extracts obtained from Ullung Is. (native site) were less active than the extracts from Daegwallyoung, suggesting that A. victorialis var. platyphyllum from the cultivated site is more useful for functional food than of native site. These results also suggest that the antihepatotoxic effect is due to a higher content of hepatic glutathione. Gas chromatography of the twelve extracts showed significantly different sulfides, disulfides or trisulfides contents belonging to volatile sulfur substances (VSS). Nine components were identified on the basis of their mass spectra, namely, dimethyl disulfide, dimethyl trisulfide, diallyl disulfide, dipropyl disulfide, allyl methyl sulfide, allyl methyl trisulfide, 2-vinyl-4H-1,3-dithiin, 3,4-dihydro-3-vinyl-1,2-dithiin, and allithiamine. Extract DL-1 had the highest VSS content. Dried plant materials contained larger amounts of the VSSs than other extracts, and the leaves contained larger amount than the bulbs. These results suggest that heat treatment increases the antiheaptotoxic ability of A. victorialis var. platyphyllum by increasing the proportion of VSSs.

• Journal of Life Science
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• v.28 no.10
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• pp.1212-1219
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• 2018

The critical role of heat shock protein 90 (Hsp90) in tumorigenesis led to the development of several first- and second-generation Hsp90 inhibitors, which have demonstrated promising responses in cancers. In this study, we found second-generation Hsp90 inhibitor BIIB021-resistant multidrug-resistant (MDR) human cancer cells, although BIIB021 was shown to be active in first-generation Hsp90 inhibitor 17-allylamino-17-demethoxygeldanamycin (17-AAG)-resistant MDR cells. MCF7-MDR and HeyA8- MDR cells were more resistant to BIIB021 than their parental counterparts, indicating that BIIB021 cannot be applicable to all cancer cells expressing MDR proteins. We revealed that dimethyl-celecoxib (DMC), one of the non-steroidal anti-inflammatory drugs (NSAIDs), potentiated cytotoxicity of BIIB021 against both BIIB021-resistant and BIIB021-sensitive MDR cells. The effectiveness of NSAIDs involving celecoxib and DMC in combination with BIIB021 led to the autophagic degradation/down-regulation of mutant p53 (mutp53) that overexpressed MDR cells and the suppression of Hsp70 induction. This resulted in sensitization of MDR cells to BIIB021. Moreover, autophagy induction by sulindac sulfide, another type of NSAID, and niclosamide, an FDA-approved anthelmintic drug, potentiated 17-AAG-mediated autophagic degradation/down-regulation of mutp53 and c-Myc, client proteins of Hsp90. Therefore, our results suggest that NSAIDs and niclosamide positively enhance the anticancer activity of Hsp90 inhibitors through an autophagic pathway. They may also be new candidates for sensitizing MDR cells to Hsp90 inhibitors.

• Applied Biological Chemistry
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• v.30 no.3
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• pp.272-277
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• 1987

The odor threshold and agreeability of aroma components in Korean Yakju were measured in Null-Yakju, 19% ethyl alcohol solution, $20^{\circ}C$ distilled water and $50^{\circ}C$ distilled water by 20 panels. The measurement of the odor threshold in Null-Yakju was as follows: Formal-dehyde 5ppm, acetaldehyde 20ppm, ethylacetate 30ppm, ethyl alcohol 5g/l, acetone 7ppm, diacetyl 0.2ppm, n-propanol 20ppm, iso-butanol 80ppm, iso-amyl alcohol 10ppm and dimethyl sulfide 2ppm were found. The most agreeability of amyl alcohol, ethyl alcohol and acetaldehyde were $80{\sim}350ppm$, $2.6{\times}10^4{\sim}1{\times}10^5ppm$ and $75{\sim}160ppm$.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.4 no.3
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• pp.139-148
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• 2000

A mass balance/photochemical modeling approach was used to evaluate the sea-to-air dimethyl sulfide (DMS) fluxes in tropical regions and part of the Southern Ocean. The flux determinations were based on 10 airborne observations by ACE 1 transit flights (i.e., Flights 4-9 and 29-32). The DMS flux values for the tropical regions ranged from 1.0 to 7.4 $\mu$mole/$m^2$/day with an average estimate of 4.2$\pm$2.3 $\mu$mole/$m^2$/day. The seasonal variations in the DMS flux predicted for the equatorial Pacific Ocean based on atmospheric DMS measurements were not entirely consistent with those derived from seawater DMS measurements were not entirely consistent with those derived from seawater DMS measurements reported in previous literature. Inhomogeneities in the DMS flux field were found to cause significant shifts in the atmospheric DMS levels even in the same sampling location. Accordingly, no definitive statement can be made at this stage regarding systematic differences or agreements in the DMS flux estimates from the two approaches. Moreover, this study strongly suggests that DMS oxidation is the most likely dominant source of SO$_2$in tropical regions, which is also supported by another set of compiled observations. Finally, these SO$_2$observations indicate that, when significant data was available for both the boundary and buffer layers, the vertical SO$_2$gradient between these two zones was primarily negative.

• Biomolecules & Therapeutics
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• v.26 no.3
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• pp.306-312
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• 2018

In a previous study, we have demonstrated that S-methylmethionine sulfonium (SMMS) confers wound-healing and photoprotective effects on the skin, suggesting that SMMS can be used as a cosmetic raw material. However, it has an unpleasant odor. Therefore, in the present study, we synthesized odor-free SMMS derivatives by eliminating dimethyl sulfide, which is the cause of the unpleasant odor and identified two derivatives that exhibited skin-protective effects: one derivative comprised (2S,4S)- and (2R,4S)-2-phenylthiazolidine-4-carboxylic acid and the other comprised (2S,4R)-, (2S,4S)-, (2R,4R)-, and (2R,4S)-2-phenyl-1,3-thiazinane-4-carboxylic acid. We performed in vitro proliferation assays using human dermal fibroblasts (hDFs) and an immortalized human keratinocyte cell line (HaCaT). The two SMMS derivatives were shown to increase hDF and HaCaT cell proliferation as well as improve their survival by protecting against ultraviolet exposure. Moreover, the derivatives regulated the expression of collagen type I and MMP mRNAs against ultraviolet exposure in hDFs, suggesting that these derivatives can be developed as cosmetic raw materials.

• Korean Journal of Food Science and Technology
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• v.28 no.5
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• pp.814-821
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• 1996

An attempt was made to determine the effects of drying methods including shady air drying, presteamed and shady air drying, microwave drying, and freeze drying on the volatile flavor components of Capsella bursapastoris. Essential oils from the samples were isolated by Simultaneous steam distillation-extraction (SDE) method using diethyl ether as solvent. Concentrated samples were analyzed by gas chromatography (GC) and combined gas chromatography-mass spectrometry (GC-MS). Respective 30, 18, 29, and 26 volatile flavor components were identified in shady air dried samples, presteamed and shady air dried samples, microwave dried samples, and freeze dried samples. The kinds and amounts of volatile flavor components evidently depended upon the drying methods. Trimethyl sulfide was regarded as the most abundant component in shady air dried samples, dimethyl trisulfide in presteamed and shady air dried samples, and phytol in microwave or freeze dried samples.

• Journal of Environmental Health Sciences
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• v.45 no.5
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• pp.465-473
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• 2019

Objectives: A variety of industries handling hazardous chemicals emit odorous substances. Based on the emission characteristics of major odor substances from the results of hazardous chemical substance emissions, we will define basic data for improving the management methods of odorous substances. Methods: A survey of hazardous pollutant emissions for 2010-2016 was conducted through the Pollutant Release and Transfer Register homepage. Eight kinds of designated odor substances (ammonia, hydrogen sulfide, dimethyl disulfide, acetaldehyde, styrene, toluene, xylene, methyl ethyl ketone) provided the study subjects. The status of chemical accidents for the target substances was analyzed using the Chemistry Safety Clearing-house system. Results: From 2010 to 2016, it was found that more than 30% of businesses that emitted odorous substances accounted for more than 50% of the total emissions of the eight substances. Emissions of xylene, toluene, methyl ethyl ketone, and ammonia were found, in that order, and they made up more than 90% of the total emitted. By region, about 70% of odorous substances were emitted in the top-four regions: Gyeongsangnam-do Province, Ulsan, Gyeonggi-do Province, and Jeollanam-do Province. Conclusion: Recently, the amount of chemical emissions has been continuously increasing, including those that can cause odor. Odorous substances can be a serious risk to the lives of local residents. Systematic research is needed for the health protection of residents.

• Journal of Microbiology and Biotechnology
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• v.31 no.6
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• pp.803-814
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• 2021

A pilot-scale biocover was constructed at a sanitary landfill and the mitigation of methane and odor compounds was compared between the summer and non-summer seasons. The average inlet methane concentrations were 22.0%, 16.3%, and 31.3%, and the outlet concentrations were 0.1%, 0.1%, and 0.2% during winter, spring, and summer, respectively. The odor removal efficiency was 98.0% during summer, compared to 96.6% and 99.6% during winter and spring, respectively. No deterioration in methane and odor removal performance was observed even when the internal temperature of the biocover increased to more than 40℃ at midday during summer. During summer, the packing material simultaneously degraded methane and dimethyl sulfide (DMS) under both moderately thermophilic (40-50℃) and mesophilic conditions (30℃). Hyphomicrobium and Brevibacillus, which can degrade methane and DMS at 40℃ and 50℃, were isolated. The diversity of the bacterial community in the biocover during summer did not decrease significantly compared to other seasons. The thermophilic environment of the biocover during summer promoted the growth of thermotolerant and thermophilic bacterial populations. In particular, the major methane-oxidizing species were Methylocaldum spp. during summer and Methylobacter spp. during the non-summer seasons. The performance of the biocover remained stable under moderately thermophilic conditions due to the replacement of the main species and the maintenance of bacterial diversity. The information obtained in this study could be used to design biological processes for methane and odor removal during summer and/or in subtropical countries.

• Journal of Animal Science and Technology
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• v.48 no.3
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• pp.453-466
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• 2006

To comply with stricter regulations provoked by increasing odor nuisance, it is imperative to practice effective odor control for sustainable livestock production. This study was conducted to assess odor and odorous compounds emitted from liquid animal manure with different treatment methods such as Fresh Manure(without treatment, FM), Anaerobic Digestion(AD) and Thermophilic Aerobic Digestion(TAD) and their application to soil. Air samples were collected at the headspace of liquid manure, upland and paddy soil, and analyzed for odor intensity and offensiveness using an olfactometry; odor concentration index using odor analyser; nitrogen-containing compound such as ammonia(NH3) using fluorescence method; and sulfur containing compounds such as hydrogen sulfide(H2S), methyl mercaptan(MeSH), dimethyl sulfide(DMS) and dimethyl disulfide(DMDS) using gas chromatography-pulsed flame photometric detector, respectively. Odor intensity, offensiveness and concentration index from TAD liquid manure was statistically lower than those from FM and AD(p<0.01). Mean concentrations of H2S, MeSH, DMS, DMDS and NH3 were 65.93ppb, 18.55ppb, 5.26ppb, 0.33ppb and 10.57ppm for liquid manure with AD; and 5.15ppb, 0.97ppb, 0.80ppb, 0.56ppb and 1.34ppm for liquid manure with TAD, respectively. More than 60% of malodorous compounds related to nitrogen and sulfur were removed by heterotrophic microorganisms during TAD treatment. When liquid manure was applied onto upland and paddy soil, NH3 removal efficiencies ranged from 51 to 94% and 22 to 91% for AD and TAD liquid manure, respectively. The above results show that liquid manure with TAD is superior to AD and FM with respect to the odor reduction and odor problem caused by land applied liquid manure is directly related to the degree of odor generated by the manure treatment method.

• Journal of the Korea Organic Resources Recycling Association
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• v.20 no.4
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• pp.118-126
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• 2012

In this study, two types of ozone generating experimental instrument were installed in commercial livestock manure fertilization facility, which can treat hundred tons of pig manure in a day. Gas samples to be treated were collected from the upper part of the liquid fertilization system and composting system of the commercial livestock manure fertilization facility. The gas sample was flowed to oxidation reactor through pipe line by suction blower, therefore, contacted with ozone. Ammonia and sulfur compounds of gas samples collected from the inlet and outlet point of the experimental instrument were analyzed. The oxidation effect by the contact with ozone was higher in sulfur compounds than ammonia. Ammonia content was reduced about 10% by ozone contact. Sulfur compounds, on the other hand, reduced significantly while treated with ozone. In case of gas sample collected from liquid fertilization system, the concentrations of hydrogen sulfide ($H_2S$), methyl mercaptan (MM), dimethyl sulfide (DMS), and dimethyl disulfide (DMDS) of inlet gas were 50.091, 4.9089, 27.8109 and 0.4683 ppvs, respectively. After oxidized by ozone, the concentrations of sulfur compounds were 1.2317, 0.3839, 14.7279 and 0.3145 ppvs, respectively. Another sample collected from aerobic composting system was oxidized in the same conditions. The concentrations of $H_2S$, MM, DMS and DMDS of the sample collected from inlet point of the reactor were 40.6682, 1.3675, 24.2458 and 0.8289 ppvs, respectively. After oxidized, the concentrations of $H_2S$, MM, DMS, and DMDS were reduced to 3.013, ND, 8.8998 and 0.3651 ppvs, respectively. By application of another type of ozone, the concentrations of $H_2S$, MM, DMS and DMDS of inlet gas were reduced from 43.397, 1.4559, 3.6021 and 0.4061 to ND, ND, ND, and 0.21ppvs, respectively.