• The Plant Pathology Journal
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• v.21 no.1
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• pp.47-54
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• 2005

Identification of host genes involved in disease progresses and/or defense responses is one of the most critical steps leading to the elucidation of disease resistance mechanisms in plants. Soybean mosaic virus (SMV) is one of the most prevalent pathogen of soybean (Glycine max). Although the soybeans are placed one of many important crops, relatively little is known about defense mechanism. In order to obtain host genes involved in SMV disease progress and host defense especially for virus resistance, two different cloning strategies (DD RT-PCR and Subtractive hybridization) were employed to identify pathogenesis- and defenserelated genes (PRs and DRs) from susceptible (Geumjeong 1) and resistant (Geumjeong 2) cultivars against SMV strain G7H. Using these approaches, we obtained 570 genes that expressed differentially during SMV infection processes. Based upon sequence analyses, differentially expressed host genes were classified into five groups, i.e. metabolism, genetic information processing, environmental information processing, cellular processes and unclassified group. A total of 11 differentially expressed genes including protein kinase, transcription factor, other potential signaling components and resistant-like gene involved in host defense response were selected to further characterize and determine expression profiles of each selected gene. Functional characterization of these genes will likely facilitate the elucidation of defense signal transduction and biological function in SMV-infected soybean plants.

• Molecular & Cellular Toxicology
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• v.5 no.3
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• pp.187-192
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• 2009

Toll-like receptors (TLRs) play an important role in host defense by sensing invading microbial pathogens. The stimulation of TLRs by microbial components triggers the activation of the myeloid differential factor 88 (MyD88)- and toll-interleukin-1 receptor domain-containing adapter inducing interferon-$\beta$ (TRIF)-dependent downstream signaling pathways. TLR/MyD88 signaling pathway induces the activation of nuclear factor-kappa B (NF-${\kappa}B$) and the expression of inflammatory cytokine genes, including tumor necrosis factor-alpha, interleukin (IL)-6, IL-12, and IL-$1{\beta}$. On the other hand, TLR/TRIF signaling pathway induces the delayed-activation of NF-${\kappa}B$ and interferon regulatory factor 3 (IRF3), and the expression of type I interferons (IFNs) and IFN-inducible genes. The divalent heavy metal cadmium (Cd) is clearly toxic to most mammalian organ systems, especially the immune system. Yet, the underlying toxic mechanism(s) remain unclear. Cd inhibits the MyD88-dependent pathway by ceasing the activity of inhibitor-${\kappa}B$ kinase. However, it is not known whether Cd inhibits the TRIF-dependent pathway. Presently, Cd inhibited NF-${\kappa}B$ and IRF3 activation induced by lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid. Cd inhibited LPS-induced IRF3 phosphorylation and IFN-inducible genes such as interferon inducible protein-10 and regulated on activation normal T-cell expressed and secreted (RANTES). These results suggest that Cd can modulate TRIF-dependent signaling pathways of TLRs.

• 한국초전도학회:학술대회논문집
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• v.10
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• pp.85-85
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• 2000

The normal state of high-Tc superconducting materials has been believed to contain important clues to finding the correct mechanism of the high-Tc superconductivity. One example is the existence of pseudogap in the normal state even above Tc, as observed in various measurements such as photoemission spectroscopy and tunneling conductance. In this pseudogap regime the existence of preformed pairs only with local phase coherence has been debated. Recently Choi, Bang, and Campbell[1] have proposed the occurrence of the zero-bias conductance enhancement due to Andreev quasiparticle reflection from the preformed pairs even with the local phase coherence. In this study we examine the zero-bias enhancement of the differential conductance near or slightly above Tc, using c-axis tunneling in mesa structure of Bi2Sr20a0u208+d single crystals. In slightly overdoped samples zero-bias conductance enhancement (ZBCE) has been observed over a range of 2 K above Tc. In contrast, in underdoped samples with Tc${\sim}$72K the ZBCE appears over a range of 5-6 K above Tc, a much wider temperature range than in overdoped samples. This result may pose as positive signs of the existence of prefurmed pairs in the normal state of high- Tc superconducting materials.

• The Bulletin of The Korean Astronomical Society
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• v.42 no.1
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• pp.45.1-45.1
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• 2017

Optical variability is one way to probe the nature of the central engine of AGN at smaller linear scales, and previous studies have shown that optical variability of AGN is more prevalent at longer timescales and at shorter wavelengths. To understand the properties and physical mechanism of variability, we are performing the KMTNet Active Nuclei Variability Survey (KANVaS). Especially, we investigated intra-night variability of AGN with KMTNet data which observed COSMOS field during 3 separate nights from 2015 to 2016 in B, V, R, and I bands. Each night was composed of 5, 9, and 11 epochs with 20-30 min cadence. To find AGN in the COSMOS field, we applied multi-wavelength selection methods. Using X-ray, mid-infrared, and radio selection methods, 50-60, 130-220, 20-40 number of AGN are detected, respectively. Achieving photometric uncertainty ~0.01mag by differential photometry, we employed a standard time-series analysis tool to identify variable AGN, chi-square test. Preliminary results indicate that there is no evidence of intra-night optical variability of AGN. It is possible that previous studies discovered intra-night variability used inappropriate photometric error. However, main reason seems that our targets have fainter magnitude (higher photometric error) than that of previous studies. To discover variability of AGN, we will investigate longer timescale variability of AGN.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.6
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• pp.895-902
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• 2012

The Korean native chickens (Woorimotdak$^{TM}$, KNC) and commercial broilers (Ross, CB) show obvious differences in meat flavor after cooking. To understand the contribution of protein and peptide for meat flavor, 2-dimensional (2-D) gel electrophoresis and matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry was performed. A total of 16 protein spots were differentially expressed in the breast and thigh meat between the two breeds. A total of seven protein spots were represented by different levels between KNC and CB for breast meat. Among them three protein spots (TU39149, TU40162 and TU39598) showed increases in their expressions in KNC while other four protein spots (BU40125, BU40119, BU40029 and BU39904) showed increases in CB. All nine protein spots that were represented by different levels between KNC and CB for thigh meat showed increases in their expression in KNC. Phosphoglucomutase 1 (PGM 1), myosin heavy chain (MyHC), heat shock protein B1 (HSP27), cytochrome c reductase (Enzyme Q), Glyoxylase 1, DNA methyltransferase 3B (DNA MTase 3) were identified as the main protein spots by MALDI-TOF mass spectrometry. These results can provide valuable basic information for understanding the molecular mechanism responsible for breed specific differences in meat quality, especially the meat flavour.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2017.05a
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• pp.140-140
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• 2017

4륜 장착 자동차의 토크 전달은 2륜 구동, 4륜 구동 모드를 간단히 전환하는 방식(part time 4WD)과 항시 사륜 구동 모드에서 전후륜의 토크 전달비를 제어하는 방식(AWD, all wheel drive)이 있다. 경제의 발달에 따라서 취미 인구의 확대로 국내에만 180만 명의 R/C car 사용자가 있다. 이 중 2WD-4WD의 전환을 differential lock mechanism으로 구현한 수입산 모델의 가격이 1,000,000원을 호가하지만 가변 제어 방식이 아닌, 정차 후 2-4륜 구동 전환 방식을 적용하고 있으며 상대적으로 내구성이 떨어진다. DC motor의 출력이 늘어나고 배터리의 성능이 좋아진 현재 소형 RC car의 최고 속도는 80 km/h 정도로 빨라졌다. 그러나 마찰 계수가 낮은 노면(실내의 대부분 평활 처리된 복도)에서는 2륜 구동 모드의 활용도가 매우 낮다. 미끄러운 노면에서 후륜 구동 모드로는 oversteer가 발생하여 차량이 스핀하기 쉽고 전륜 구동 모드로는 understeer가 발생하여 제대로 된 코너링이 어렵다. 상시 4륜 구동 모드는 에너지 소모가 크고 전후륜이 tight coupling되어 있는 문제 때문에 일반적인 노면에서 부드러운 코너링이 잘 이루어지지 않는 문제가 있다. 본 연구에서 제안하는 방식은 그림 1와 같이 center shaft의 중간에 영구 자석으로 만들어진 토크 전달용 판이 있고 그 사이에 자계를 차폐할 수 있는 강자성체 셔터를 서보 기구에 연결하여 서보 회전각에 따라서 구동 쪽의 토크가 피구동축으로 전달되는 양을 연속 가변제어할 수 있다. 토크 전달용 판의 차폐 면적에 따른 토크 전달양을 전/후륜 바퀴의 Static torque를 통해 측정하였으며(그림 2), 공중 상태에서 즉 공기저항만을 고려한 상태에서의 RPM 회전수 차이 측정(그림3)을 통해 구동 쪽의 회전수가 피구동축으로 전달되는 양을 측정하여 연속가변 토크 제어 전달 기구의 성능을 확인하였다. 이 기구는 현재 1차적으로는 remote controller의 ch 3(ON/OFF제어 방식)에 연결하여 특정한 양의 토크를 전륜 쪽으로 보낼 수 있도록 구현이 가능하며, ch 2(PID제어 방식)에 연결하여 연속 가변 조절이 가능하도록 구현이 가능하다. 부가적으로 Arduino board를 내장하여 전후륜의 휠센서에서 입력되는 신호를 감지하여 자동적으로 전후륜에 배분되는 토크를 제어할 수 있도록 설계 중에 있다.

• Journal of the Korea Concrete Institute
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• v.21 no.5
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• pp.611-617
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• 2009

This paper reports an experimental study on the damage mechanism and resistance of Type I portland cement mortar and paste samples exposed to 5% sodium sulfate solution with different solution temperatures; namely, $4^{\circ}C$, $10^{\circ}C$ and $20^{\circ}C$. The resistance of mortar samples was evaluated using expansion, compressive strength and flexural strength measurements. Some microstructural observations such as x-ray diffraction, differential scanning calorimetry and scanning electron microscopy were also introduced to elucidate reactants formed by sulfate attack, especially in a low temperature condition. From the results, it was found that the degree of damage in the mortar samples was significantly associated with the temperature of sulfate solution. Low temperature of the sulfate solution led to the formation of thaumasite in mortar and paste samples, and subsequently a poor resistance to sulfate attack. Thus, it is noted that when concrete structures are exposed to sulfate media in the condition of a cold region or whether, special care should be taken.

• Clinical and Experimental Pediatrics
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• v.51 no.11
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• pp.1140-1146
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• 2008

Nocturnal enuresis is a heterogeneous disorder with various underlying pathophysiological mechanisms and causes a mismatch between the nocturnal bladder capacity and the amount of urine produced during sleep at night. It is associated with a simultaneous failure of conscious arousal in response to the sensation of bladder fullness. Generally, a complete history and physical examination, with a specific focus on the genitourinary, gastrointestinal, and neurologic systems, is sufficient to evaluate a patient with enuresis. The therapeutic focus is directed toward a differential approach based on the underlying mechanism and toward combination therapies such as alarm devices and desmopressin as well as anticholinergic agents and desmopressin. Children with increased nocturnal urine production usually have a good response to desmopressin therapy. Patients with a small bladder generally show a poor response to desmopressin treatment, but they would benefit more from combination therapy with enuretic alarm, urotherapy, and antimuscarinic agents in addition to desmopressin. Different types of bladder dysfunction, which result in a small nocturnal bladder capacity, probably contribute significantly to the pathogenesis of nocturnal enuresis, particularly in those with treatment failure and refractory symptoms. Because different clinical subgroups may show different responses to treatment, it is necessary to distinguish these subgroups before a decision on the specific treatment protocol can be made.

• The Korean Journal of Physiology and Pharmacology
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• v.7 no.3
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• pp.125-130
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• 2003

The aim of this study was to provide a basis for studying the molecular mechanism of pharmacological action of chlorhexidine digluconate. Fluorescence polarization of n-(9-anthroyloxy)stearic acid was used to examine the effect of chlorhexidine digluconate on differential rotational mobility of different positions of the number of membrane bilayer phospholipid carbon atoms. The six membrane components differed with respect to 2, 3, 6, 9, 12, and 16-(9-anthroyloxy)stearic acid (2-AS, 3-AS, 6-AS, 9-AS, 12-AS and 16-AP) probes, indicating different membrane fluidity. Chlorhexidine digluconate increased the rate of rotational mobility of hydrocarbon interior of the cultured Porphyromonas gingivalis outer membranes (OPG) in a dose-dependent manner, but decreased the mobility of surface region (membrane interface) of the OPG. Disordering or ordering effects of chlorhexidine digluconate on membrane lipids may be responsible for some, but not all of its bacteriostatic and bactericidal actions.

• The Korean Journal of Physiology and Pharmacology
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• v.7 no.3
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• pp.119-124
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• 2003

To elucidate the molecular mechanism of pharmacological action of local anesthetics, we studied membrane actions of tetracaine, bupivacaine, lidocaine, prilocaine and procaine. Fluorescence polarization of n-(9-anthroyloxy)stearic acid (n-AS) was used to examine the effects of these local anesthetics on differential rotational mobility of different positions of the number of synaptosomal plasma membrane vesicle (SPMV) phospholipid carbon atoms. The four membrane components differed with respect to 3, 6, 9 and 16-(9-anthroyloxy)stearic acid (3-AS, 6-AS, 9-AS and 16-AP) probes, indicating that differences in the membrane fluidity might be present. Degrees of the rotational mobility of 3-AS, 6-AS, 9-AS and 16-AP were different depending on depth of hydrocarbon interior. In a dose-dependentmanner, tetracaine, bupivacaine, lidocaine, prilocaine and procaine decreased anisotropy of 3-AS, 6-AS, 9-AS and 16-AP in the hydrocarbon interior of the SPMV. These results indicate that local anesthetics have significant disordering effects on hydrocarbon interior of the SPMV, thus affecting the transport of $Na^+$ and $K^+$ in nerve membranes and leading to anesthetic action.