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Effect of Planting Density and Fertilizer Application Level on Yield and Agronomic Characters in a Semi-dwarf Soybean Cultivar (재식밀도와 시비량이 단경종 콩의 주요 생육형질과 수량에 미치는 영향)

  • 박춘봉;정진욱;황창주;소재돈;박노풍
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.1
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    • pp.73-82
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    • 1990
  • The experiment was carried out to study the influence of planting space and fertilizer application levels on some agronomic characters and yield in newly released semi-dwarf soybeans, Paldalbong and Dankyungkong. The number of branches per plant was not reduced by planting space from 60${\times}$10cm 30${\times}$15cm. These Semi-dwarf soybeans had good adaptability on high planting density. The number of pods per node on the main stem was different according to the planting space in Dankyungkong, but it was similar in Paldalkong except planting space 20${\times}$10cm. Regardless of planting densities and varieties, the number of grains per pod on upper node of the main stem was large. Coefficient of variation of pods and grains per node in the middle part of the main stem was small. The number of grains per plant was decreased in high planting density compared to low planting density, but the number of harvested plant per unit area was increased. Optimum planting space was 25${\times}$10cm in both varieties. The ability of nodulation was bigger in Dankyungkong than in Paldalkong.

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Evaluation of Pepper Genetic Sources (Capsicum spp.) for Disease Resistance Breeding (병저항성 육종을 위한 고추 유전자원의 저항성 평가)

  • Lee, Sang-Jun;Kim, Byung-Sup
    • Horticultural Science & Technology
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    • v.30 no.2
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    • pp.185-191
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    • 2012
  • For initiation of resistance breeding program of the red pepper, 21 PR ($Phytophthora$ resistance) cultivars, 36 cultivars collected from USA and 'Supermanitta' which is a susceptible cultivar against phytophthora blight were assayed against phytophthora blight, powdery mildew, and anthracnose. For seedling assay of phytophthora blight, three different mating type strains of $Phytophthora$ $capsici$ were used (A1, A2, Sterile). The result showed that most of the pepper of PR cultivars were resistance or moderately resistance at each mating type. 'Yeokganghongjanggun' was resistant to all three $P.$ $capsici$ strains and 'PR-Datta' and 'PR-Manitta' were resistant or moderately resistant at each type. In case of the collected cultivars, 'NuMex J.E.Parker', 'Omni Color', and 'SCM334' were resistant to all the three types and some cultivars including 'Sweet Banana' and 'Tabasco' were moderately resistant to each type fungi. 'Orange Habanero' and 'Black Cuban' were resistant to powdery mildew and 'Supermanitta' and 'PR Keumdong' were moderately resistant, while 'Santa Fe Grande', 'NuMex Pinata' and 'Puya' were very susceptible. In the case of anthracnose, 'Aji Limon' and 'Capsicum baccatum var. pendulum 3-4' were resistant and 'Pobalno', 'Omni Color', 'Negro', 'Mesilla', 'Mulato', 'Bhut Jolokia', 'Big Dipper', 'Black Cuban', 'NuMex Pinata', and 'NuMex Big Jim' were moderately resistant. The most PR cultivars except 'Taesan' were susceptible or very susceptible. These resistant individuals identified through this experiment can be used as sources of resistance to pepper pathogens in the future breeding programs.

Effect of Two Male Cultivars of Asparagus with Low Temperature Treatment on Bud Breaking and Spear Growth (아스파라거스 두 전웅 품종의 저온처리에 따른 휴면 타파와 순의 생장에 미치는 영향)

  • Lee, Jeong Hyun;Bae, Jong Hyang;Ku, Yang Gyu
    • Horticultural Science & Technology
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    • v.31 no.2
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    • pp.141-145
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    • 2013
  • The aim of this study was to investigate the effect of two male cultivars of asparagus with low different chilling periods on bud breaking, relative spear growth rate, shoot number and yield. Four-month-old plants of two male cultivars of asparagus were dried to impose dormancy and placed in a coldroom at $5^{\circ}C$ to satisfy the chilling requirement for 0, 2, 4, and 6 weeks. After the specified chilling time, the pots were placed in greenhouse. The results showed that mean days to bud break of 'Jersey Giant' significantly decreased with an increasing chilling period from 0 to 6 weeks, but 'Jersey Supreme' was not affected. The relative spear growth rate of 'Jersey Supreme' was significantly stimulated by chilling compared to non-chilled plants. Shoot production and total spear weight of 'Jersey Supreme' tended to be greater than 'Jersey Giant' irrespective of chilling treatments. Following low temperature treatment, 'Jersey Supreme' showed shorter dormant period than 'Jersey Giant', faster growth of spear. The present study showed that asparagus male cultivar at duration of chilling treatment had an affirmative effect on bud breaking, spear growth rates, shoot number and yield of asparagus.

Bioactive Component Analysis and Antioxidant Activity in Fresh Sprout of New Kalopanax septemlobus Cultivar (음나무 신품종 새순의 유용물질 함량분석과 항산화활성)

  • Song, Jeong-Ho;Kim, Hyeusoo;Kim, Moon-Sup;Kim, Sea-Hyun;Lee, Jin-Tae
    • Journal of Korean Society of Forest Science
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    • v.104 no.3
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    • pp.397-402
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    • 2015
  • This study was carried out to analyze the nutritional composition, bioactive compound and antioxidant activity in fresh sprout of 3 new Kalopanax septemlobus cultivars. Cheong-San, Cheong-Song and Cheong Sun 1 had largest carbohydrate (52.90, 53.12, 55.28%), crude protein (34.23, 31.33, 33.58%) in the proximate composition and had largest K (2.29, 2.22, 2.23%) followed by Ca (0.44, 0.34, 0.37%), Mg (0.24, 0.19, 0.19%) in the mineral contents. Three new cultivars contained a total of 26, 25 and 28 different kinds of amino acids, respectively and the number of essential amino acid is 6, 6 and 7 species, respectively. Also, Cheong-San had higher total polyphenol contents and Cheong-Song had higher total flavonoid contents. DPPH free radical scavenging activity of 70% ethanol, water extracts of Cheong-San and Cheong-Song is higher than others at 100 ppm. In the ABTS radical cation decolorization activity, 70% ethanol extract of Cheong Sun 1, water extract of Cheong-San and Cheong-Song are higher activity than others and all samples have more than 85% ABTS racial cation decolorization activity at 500 ppm. Our results suggest that new K. septemlobus cultivars possess good antioxidant capacities with a high nutritional value and might have potential applications in the food and medical industries.

A simple screening method using lignoceullulose biodegradation for selecting effective breeding strains in Agaricus bisporus (리그노셀룰로오스 생물학적 분해를 이용한 간단한 양송이 육종효율 우수 균주 선발)

  • Oh, Youn-Lee;Nam, Youn-Keol;Jang, Kab-Yeul;Kong, Won-Sik;Oh, Min ji;Im, Ji-Hoon
    • Journal of Mushroom
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    • v.15 no.3
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    • pp.134-138
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    • 2017
  • The white button mushroom, Agaricus bisporus, is commercially the fifth most important edible mushroom, accounting for the production of 9,732 tons of mushrooms in Korea in 2015. The genus Agaricus has been known for its potential to degrade lignocellulosic materials. Chemical analyses carried out during the cultivation of A. bisporus indicated that the cellulose, hemicellulose, and lignin fractions were changed preferentially for both vegetative growth and sexual reproduction. We screened A. bisporus strains for effective biodegradation through extracellular enzyme activity using cellulase, xylanase, and ligninolytic enzymes. The enzyme biodegradations were conducted as follows: mycelia of collected strains were incubated in 0.5% CMC-MMP (malt-mops-peptone), 0.5 Xylan-MMP, and 0.5% lignin-MMP media for 14 days. Incubated mycelia were stained with 0.2% trypan blue. Eighteen strains were divided into 8 groups based on different extracellular enzyme activity in MMP media. These strains were then incubated in sterilized compost and compost media for 20 days to identify correlations between mycelial growth in compost media and extracellular enzyme activity. In this study, the coefficient of determination was the highest between mycelial growth in compost media and ligninolytic enzyme activity. It is suggested that comparison with ligninolytic enzyme activity of the tested strains is a simple method of screening for rapid mycelial growth in compost to select good mother strains for the breeding of A. bisporus.

Identification of fungal races that cause powdery mildew in melon (Cucumis melo L.) and selection of resistant commercial melon cultivars against the identified races in Korea (국내 멜론 흰가루병균의 race 동정 및 시판품종의 흰가루병 저항성 판별)

  • Kim, Hoy-taek;Park, Jong-in;Nou, Ill-sup
    • Journal of Plant Biotechnology
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    • v.43 no.1
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    • pp.58-65
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    • 2016
  • Powdery mildew is an important disease of the melon (Cucumis melo L.). Seven isolates of powdery mildew fungi were collected from five locations in Korea; Anseong (DH487), Icheon (BN103, BN625, BN968), Yeongam (YA141), Changnyeong (CN582), and Suncheon (SN102). All 7 fungi had a similar trend of conidial chain and conidiophore development as Podosphaera xanthii with fibrosin bodies in mature conidia. Among them, 2 isolates of powdery mildew fungi; CN582 and SN102 showed similar responses to resistance against powdery mildew as the previously reported race 1 and race N2. The isolates YA141 and BN103 showed similar responses as like as race A. However, three isolates of powdery mildew fungi (BN625, BN968, and DH487) showed different responses compared to the previously reported races (1, N1, N2, A, S, and 5). Therefore, these three isolates could be designated as new races in melon. Nine out of 15 commercial melon cultivars in Korea showed resistance to race 1 (CN582). However, the new race BN968 invaded all 15 cultivars. Results of the two molecular markers were consistent in response to disease development by race 1 of Podosphaera xanthii in case of the above mentioned cultivars. This study confirmed the presence of new melon powdery mildew fungi in Korea which are similarly notorious as like as the previously reported race 1. Therefore, breeders can use these two molecular markers for breeding melon in Korea that is resistant to race 1 and as well as to some other races.

Cloning and Phylogenetic Characterization of Coat Protein Genes of Two Isolates of Apple mosaic virus from ¡?Fuji¡? Apple

  • Lee, Gung-Pyo;Ryu, Ki-Hyun;Kim, Hyun-Ran;Kim, Chung-Sun;Lee, Dong-Woo;Kim, Jeong-Soo;Park, Min-Hye;Noh, Young-Mi;Choi, Sun-Hee;Han, Dong-Hyun;Lee, Chang-Hoo
    • The Plant Pathology Journal
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    • v.18 no.5
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    • pp.259-265
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    • 2002
  • Apple mosaic virus (ApMV), a member of the genus Ilarvirus, was detected and isolated from diseased 'Fuji' apple (Malus domestica) in Korea. The coat protein (CP) genes of two ApMV strains, denoted as ApMV-Kl and ApMV-K2, were amplified by using the reverse transcription and polymerase chain reaction (RT-PCR) and were analyzed thereafter. The objectives were to define the molecular variability of genomic information of ApMV found in Korea and to develop virus-derived resistant gene source for making virus-resistant trans-genic apple. RT-PCR amplicons for the APMVS were cloned and their nucleotide sequences were determined. The CPs of ApMV-Kl and ApMV-K2 consisted of 222 and 232 amino acid residues, respectively. The identities of the CPs of the two Korean APMVS were 93.1% and 85.6% at the nucleotide and amino acid sequences, respectively. The CP of ApMV-Kl showed 46.1-100% and 43.2-100% identities to eight different ApMV strains at the nucleotide and amino acid levels, respectively. When ApMV-PV32 strain was not included in the analysis, ApMV strains shared over 83.0% and 78.6% homologies at the nucleotide and amino acid levels, respectively. ApMV strains showed heterogeneity in CP size and sequence variability. Most of the amino acid residue differences were located at the N-termini of the strains of ApMV, whereas, the middle regions and C-termini were remarkably conserved. The APMVS were 17.(1-54.5% identical with three other species of the genus Ilarviyus. ApMV strains can be classified into three subgroups (subgroups I, II, and III) based on the phylogenetic analysis of CP gene in both nucleotide and amino acid levels. Interestingly, all the strains of subgroup I were isolated from apple plants, while the strains of subgroups II and III were originated from peach, hop, or pear, The results suggest that ApMV strains co-evolved with their host plants, which may have resulted in the CP heterogeneity.

Growth, Floral Morphology, and Phytohormone Levels of Flowering Shoots with Bent Peduncle in Greenhouse-grown Cut Rose 'Beast' ('Beast' 장미 꽃목굽음지의 생장, 화기 및 내생 호르몬 변화)

  • Seo, Ji Hyeon;Kim, Wan Soon
    • Horticultural Science & Technology
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    • v.31 no.6
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    • pp.714-719
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    • 2013
  • The bent peduncle phenomenon (BPP) is known as a kind of physiological disorders found in cut rose plants, which causes the reduction of cut flower yields. As yet unknown mechanisms, however, it has not been easy to find solutions for BPP. To address this challenge, this study was conducted to investigate the characteristics of floral morphology, growth, and endogenous phytohormone level of BPP shoots in cut rose plants (Rosa hybrida L. 'Beast'). Morphological observation confirmed the fact that BPP was accompanied by the early formation and being phyllody of a specific sepal among five sepals, which gave rise to peduncle bending in the upper part of the phyllody with fasciation. Year-round BPP frequency in 'Beast' cultivar was in the range of 5 to 20% with seasonal change, increasing rapidly with an average temperature rise in summer. Except bent peduncle, the BPP shoots appeared to grow normally in terms of speed and size of floral development in comparison with normal. However, carbohydrate distribution to the floral part in BPP shoots was significantly reduced. The level of endogenous IAA (3-Indoleacetic acid) within the floral part in BPP shoots was highly maintained during floral development, in contrast to normal shoots. $GA_3$ contents were not significantly different between normal and BPP shoots. This study indicates that BPP would be induced by a series of courses: abnormally early formation of a specific sepal relative to high temperature, being phyllody of the sepal with fasciation, and continuous supply of endogenous IAA by phyllody.

Development of Sequence Characterized Amplified Region Markers for Cultivar Identification in Persimmon (감 품종 판별용 SCAR 마커 개발)

  • Cho, Kang Hee;Cho, Kwang-Sik;Han, Jeom Hwa;Kim, Hyun Ran;Shin, Il Sheob;Kim, Se Hee;Chun, Jae An;Hwang, Hae-Sung
    • Horticultural Science & Technology
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    • v.31 no.6
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    • pp.798-806
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    • 2013
  • Precise, fast, and cost-effective identification of crop cultivars is essential for plant breeder's rights. Traditional methods for identification of persimmon cultivars are based on the evaluation of sets of morphological characteristics. However, it is difficult to distinguish closely related cultivars using only morphological traits. This study was conducted to develop DNA markers for identification of the 32 persimmon cultivars in Korea and Japan. A total of 309 randomly amplified polymorphic DNA (RAPD) markers were identified using 40 different random primers. Various number of polymorphic bands ranged from 4 (OPP-08) to 14 (UBC159) were detected with an average of 7.7. Resulting 57 RAPD fragments were selected, and their sequences were determined for developing sequence characterized amplified region (SCAR) markers. As a result, 15 of 57 RAPD fragments were successfully converted to SCAR markers. Single polymorphic bands of the same size as or smaller than the RAPD fragments were amplified depending on SCAR markers. Among these markers, a combination of eight SCAR markers (PS225_200, PSN05_420, PSF13_523, PSN11_540, PS372_567, PS485_569, PSP08_635, and PS631_735) provided sufficient polymorphisms to identify 32 persimmon cultivars. These newly developed markers will be a fast and reliable tool to identify persimmon cultivars.

Changes in Some Characteristics of Brined Chinese Cabbage of Fall Cultivars During Storage (품종별 가을배추로 제조한 절임배추의 저장중 특성변화)

  • Lee, In-Seon;Park, Wan-Soo;Koo, Young-Jo;Kang, Kook-Hee
    • Korean Journal of Food Science and Technology
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    • v.26 no.3
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    • pp.239-245
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    • 1994
  • Fermentation characteristics and microbial changes during storage of brined Chinese cabbage were investigated. Ten Chinese cabbage cultivars harvested in fall were brined and stored at $0^{\circ}C$ and $10^{\circ}C$. The decrease in pH and increase in total acidity during storage were different in their tendency among the varieties. Microbial growth including lactic acid bacteria was more active at $10^{\circ}C$ and most active in `Noranmat'. The reduction of their reducing sugar contents during storage resulted in slow fermentation of kimchi prepared with those cabbage stored. From the results of sensory evaluation on kimchi prepared with each brined Chinese cabbage cultivar in storage, `Keunyureum' among the cultivats stored at $0^{\circ}C$ received the highest points on preference. But, at $10^{\circ}C$, `Sinkiwon' received the highest points and 'Noktab' received the lowest points.

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