Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
권호 표지
DOI QR코드

DOI QR Code

Bioactive Component Analysis and Antioxidant Activity in Fresh Sprout of New Kalopanax septemlobus Cultivar

음나무 신품종 새순의 유용물질 함량분석과 항산화활성

  • Song, Jeong-Ho (Division of Special Purpose Trees, Korea Forest Research Institute) ;
  • Kim, Hyeusoo (Division of Special Purpose Trees, Korea Forest Research Institute) ;
  • Kim, Moon-Sup (Division of Special Purpose Trees, Korea Forest Research Institute) ;
  • Kim, Sea-Hyun (Division of Special Purpose Trees, Korea Forest Research Institute) ;
  • Lee, Jin-Tae (Department of Cosmeceutical Science, Daegu Haany University)
  • 송정호 (국립산림과학원 특용자원연구과) ;
  • 김혜수 (국립산림과학원 특용자원연구과) ;
  • 김문섭 (국립산림과학원 특용자원연구과) ;
  • 김세현 (국립산림과학원 특용자원연구과) ;
  • 이진태 (대구한의대학교 화장품약리학과)
  • Received : 2015.05.13
  • Accepted : 2015.06.24
  • Published : 2015.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to analyze the nutritional composition, bioactive compound and antioxidant activity in fresh sprout of 3 new Kalopanax septemlobus cultivars. Cheong-San, Cheong-Song and Cheong Sun 1 had largest carbohydrate (52.90, 53.12, 55.28%), crude protein (34.23, 31.33, 33.58%) in the proximate composition and had largest K (2.29, 2.22, 2.23%) followed by Ca (0.44, 0.34, 0.37%), Mg (0.24, 0.19, 0.19%) in the mineral contents. Three new cultivars contained a total of 26, 25 and 28 different kinds of amino acids, respectively and the number of essential amino acid is 6, 6 and 7 species, respectively. Also, Cheong-San had higher total polyphenol contents and Cheong-Song had higher total flavonoid contents. DPPH free radical scavenging activity of 70% ethanol, water extracts of Cheong-San and Cheong-Song is higher than others at 100 ppm. In the ABTS radical cation decolorization activity, 70% ethanol extract of Cheong Sun 1, water extract of Cheong-San and Cheong-Song are higher activity than others and all samples have more than 85% ABTS racial cation decolorization activity at 500 ppm. Our results suggest that new K. septemlobus cultivars possess good antioxidant capacities with a high nutritional value and might have potential applications in the food and medical industries.

본 연구는 국립산림과학원에서 음나무 신품종으로 개발한 청산, 청송, 청순1 등 3개 신품종을 대상으로 새순의 영양성분 분석, 생리활성 물질분석 및 항산화활성 분석을 통하여 식품학적 가치의 확인과 기능성 소재로서의 개발 가능성을 모색하고자 하였다. 3개의 품종에 대하여 일반성분은 탄수화물과 단백질 함량이 높게 나타났으며 무기성분에서는 칼륨, 칼슘, 마그네슘 순으로 높게 나타났으나 그 차이는 미비했다. 유리아미노산 분석에서는 청산, 청송, 청순1에서 각각 26, 25, 28개의 아미노산을 분석하였으며, 각각 6, 6, 7개의 필수 아미노산을 분석하였다. 기능성 분석을 위한 총폴리페놀 함량에서는 청산 품종이, 총플라보노이드 함량에서는 청송 품종이 높게 나타났다. DPPH 자유라디컬을 이용한 항산화활성 분석에서는 100 ppm에서 청산과 청송의 70% 에탄올과 물추출물의 활성이 높게 나타났다. ABTS 라디컬을 이용한 항산화활성 분석에서는 500 ppm에서 청순1의 70% 에탄올 추출물과 청산, 청송의 물 추출물의 활성이 높게 나타났다. 본 결과로 청산, 청송의 경우 항산화활성이 우수한 기능성과 동시에 가시가 없거나 적은 형태적 특성을 활용할 수 있을 것으로 판단되어지며, 청순1의 경우 단맛을 나타내는 아미노산의 함량이 높아 음나무 고유의 쓴맛과 동시에 단맛이 나는 맛 특성을 활용할 수 있을 것으로 판단되어진다.

Keywords

References

  1. Abeysinghe, D.C., Li, X., Sun, C., Zhang, W., Zhou, C., and Chen, K. 2007. Bioactive compounds and antioxidant capacities in different edible tissues of citrus fruit of four species. Food Chemistry 104: 1338-1344. https://doi.org/10.1016/j.foodchem.2007.01.047
  2. AOAC (Association of Official Analytical Chemists). 1980. Official methods of analysis. 14h ed. Association of official analytical chemists, Washington DC.
  3. Boo, H.O., Lee, H.H., Lee, J.W., Nwang, S.J., and Park, S.U. 2009. Different of total phenolics and flavonoids, radical scavenging activities and nitrite scavenging effects of Momordica charantia L. according to cultivars. Korean Journal of Medicinal Crop Science 17: 15-20.
  4. Brand-Williams, W., Cuvelier, M., and Berset, C. 1995. Use of free radical method to evaluate antioxidant activity. LWT-Food Science and Technology 28(1): 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
  5. Choi, H.-J., Kim, D.-H., Ching, H.-S., and Moon, K.-D. 2012. Food nutritional composition of Castor Aralia (Kalopanax pictus N) sprouts. Korean Journal of Food Preservation 19(5): 720-726. https://doi.org/10.11002/kjfp.2012.19.5.720
  6. Gulcin, I., Sat, I.G., Beydemir, S., Elmastas, M., and Kufre-vioglu, O.I. 2004. Comparison of antioxidant activity of clove (Eugenia caryophylata Thunb.) buds and lavender (Lavandula stoechas L.). Food Chemistry 87: 393-400. https://doi.org/10.1016/j.foodchem.2003.12.008
  7. Hu, W., Huang, C., and Wang, M.-H. 2012. Chemical composition, nutritional value, and antioxidant constituents of Kalopanax pictus leaves. Food Chmistry 131: 229-255.
  8. Im, D.Y. and Lee, K.I. 2013. Antioxidative activity and tyrosinase inhibition effect of ethanol extract and its fractions from the branch of Rhododendrom schlippenbachii. Korean Journal of Medicinal Crop Science 21(6): 439-443. https://doi.org/10.7783/KJMCS.2013.21.6.439
  9. Im, H.-J., Jang, H.-L., Jeong, Y.-J., and Yoon, K.-Y. 2013. Chemical properties and antioxidant activities of the sprouts of Kalopanax pictus, Cedrela sinenesis, Acanthopanax cortex at different plucking times. Korean Journal of Food Preservation 20(3): 356-364. https://doi.org/10.11002/kjfp.2013.20.3.356
  10. Joo, S.Y. 2013. Antioxidant activity of medicinal plant extracts. Journal of the Korean society of food science and nutrition 42(4): 512-519. https://doi.org/10.3746/jkfn.2013.42.4.512
  11. Jun, D.-H., Lee, J.-T., Cheon, S.-J., Lee, C.-E., Kim, T.H., Lee, D.-H., Han, J., and Kim, S.-H. 2009. Polyphenol and antioxidant effects of Kalopanax septemlobus Koidz. leaf extracts. Korean Journal of Plant Research 22(4): 343-348.
  12. Jung, Y.-H. 2012. Industrialization research of Jeju medicinal plants. Food Industry and Nutrition 17(1): 13-16.
  13. Kato, H., Rhue, M.R., and Nishimura, T. 1989. Role of free amino acids and peptides in food taste. American Chemical Society 388: 158-174.
  14. Kim, M.Y., Yoo, Y.M., Nam, J.H., Choi, J.W., and Park, H.J. 2007. Quantitative determination on the constituents of the stem bark and the leaf shoot of Kalopanax pictus by HPLC analysis. Korean Journal of Pharmacognosy 38: 270-276.
  15. Kim, H.-J., Kim, M.-J., Oh, S.-I., Hwangbo, M.-H., Jang, S.-J., Kim, H.-I., and Lee, I.-S. 2012. Antioxidant activity of Kalopanax pictus leaf extract and its effects on the quality characteristics of fried pork skin. Korean Journal of Food Science and Technology 44(2): 185-190. https://doi.org/10.9721/KJFST.2012.44.2.185
  16. Kim, H.S., Kim, T.W., Kim, D.J., Lee, J.S., Kim, K.K., and Choe, M. 2013. Antioxidant activities and $\alpha$-glucosidase inhibitory effect of water extracts from medicinal plants. Korean Journal of Medicinal Crop Science 21(3): 197-203. https://doi.org/10.7783/KJMCS.2013.21.3.197
  17. Kim, M.J., Kim, J.S., Kang, W.H., and Yeon, K.D. 2002. Antimutagenic and cytotoxic effects of extract of Kalopanax pictus Nakai endodermis. Korean Journal of Crop Science 10(2): 132-138.
  18. Kim, S.-H., Park, Y., Jang, Y.-S., Han, J., and Chung, H.-G. 2007. Oxidative stress in the cell and antioxidant activity of Kalopanax pictus extracts. Mokchae Konghak 35(6): 126-134.
  19. Korean Forest Research Institute. 2010. Culture and distribution of Mountain Medical Resources (II). Korea.
  20. Nijveldt, R.J., Van Nood, E., Van Hoom, D.E., Boelens, P.G., Van Norren, K., and Van Leeuwen, P.A. 2001. Flavonoids: a review of probable mechanism of action and potential applications. The American Journal of Clinical Nutrition 74(4): 418-425.
  21. Park, H.-J., Kang, S.-A., Lee, J.-Y., and Cho, Y.-J. 2012. Antioxidant activities of extracts from medicinal plants. Korean Journal of Food Preservation 19(5): 744-750. https://doi.org/10.11002/kjfp.2012.19.5.744
  22. Park, H.-J., Nam, J.-H., Jung, H.-J., Kim, W.-B., Park, K.-K., Chung, W.Y., and Choi, J. 2005. In vivo antinociceptive antiinflamatory and antioxidative effects of the leaf and stem bark of Kalopanax pictus in Rats. Korean Journal of Phamacognosy 36(4): 318-323.
  23. Roterta, R., Nicoletta, P., Anna, P., Ananth, P., Min, Y., and Catherine, R.E. 1999. Antioxidant activity applying an improved ABTS radicalcation decolorization assay. Free radical Biology & Medicine 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  24. Rural Resources Development Institute. 2011. Standard food composition table 8th ed. Rural Resources Development Institute RDA, Suwon, Korea.
  25. Seo, K.H., Seo, J.S., Lee, B.H., Lee, S.K., and Choi, M.S. 2011. New advanced nutrition, Gigu publishing Co, Paju, Korea.
  26. Shin, E.H. 2010. Component analysis and antioxidant activity of Kalopanax pictus leaf. Journal of Korean Society of Food Science and Nutrition 39(11): 1634-1639. https://doi.org/10.3746/jkfn.2010.39.11.1634
  27. Shou, H. 1969. Food component and taste. Journal of Food Industry of Japan 16: 83-87.
  28. Yun, S.I., Choi, W.-J., Choi, Y.-D., Lee, S.-H., Yoo, S.-H., Lee, E.-J., and Ro, H.-M. 2003. Distribution of heavy metals in soils of Shihwa tidal freshwater marshes. Korean Journal of Ecology 26(2): 65-70. https://doi.org/10.5141/JEFB.2003.26.2.065