• Journal of Photoscience
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• 제9권2호
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• pp.13-16
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• 2002

A comparison of the functional components underlying photoperiodism and circadian rhythmicity in the same species is an interesting issue in the context of unravelling clock mechanisms. In the present study, covering or surgical removal of the compound eyes was performed to localize photoreceptors for photoperiodism to control reproductive diapause and for entrainment of circadian locomotor rhythms in the blow fly Protophormia terraenovae. Intact flies showed a long-day photoperiodic response. When the compound eyes were covered by silver paint, diapause incidence increased under diapause-averting conditions of a long-day photoperiod and constant light, as if flies were kept under constant darkness. Covering of a medial region of the head capsule or solvent painting of the compound eyes gave no significant effects. When the compound eyes were removed, flies did not distinguish the photoperiod, whereas removal of antennal lobes or ocelli did not affect the photoperiodism. Intact flies showed a freerunning rhythm under constant darkness. The rhythm entrained to light-dark (LD) cycles with light of high and low intensity. When the compound eyes and ocelli were surgically removed, the rhythm entrained to LD cycles with light of high intensity but freeran under LD cycles with light of low intensity. The results suggest the retinal pathways are involved in photoperiodism and that flies use both retinal and extraretinal pathways for rhythm entrainment. Under dim light-LD cycles, the retinal pathways mainly mediate rhythm entrainment. Retinal photoreceptors seem to be used both for photoperiodism and entrainment of the rhythm.

• International Journal of Industrial Entomology and Biomaterials
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• 제10권1호
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• pp.45-49
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• 2005

Respiration in Antheraea mylitta was studied using constant pressure respirometer. Oxygen uptake in different stages of life showed that moth has highest rate of oxygen uptake as compared to larva and pupa. At each stage significant sexual differentiation was observed. Depression in the rate of oxygen uptake in diapausing pupa was found to be higher in male ($65.75\%$) than female ($60.65\%$) as compared to non-diapausing counterparts. During diapause lowest oxygen uptake was recorded in the month of February, and were in the order of February < January < December < March < November < April < May < June in male, and, February < January < December < March < April < November < May < June in female. Significant sexual differentiation in oxygen uptake was recorded throughout the period of diapause (November to June) where female pupae registered lower rate of oxygen uptake than that of male. Oxygen uptake in female pupae declined upto $28.89\%$ in non-diapausing and $18.29\%$ in diapausing broods over male. Highest respiratory quotient value was recorded in the moth of November (0.68 in male and 0.70 in female) and, the lowest in the month of March in male (0.54) and May in female (0.55). Percentage loss in pupa weight always remained at a significantly higher level in male (except in February and March). The lowest oxygen uptake rate and weight loss in the month of February shows that the pupae were at peak of their dormancy during this month.

• 생명과학회지
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• 제6권3호
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• pp.171-177
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• 1996

It was carried out to find the carbohydrate metabolism for the glycogen utilization and the effects of low temperature(1$\circ$C, 5$\circ$C)on embryonic development of the silkworm, Bombyx mori L. The content of sorbitol and glycerol in the chillihg eggs stored at 5$\circ$C or 1$\circ$C on 24 hours after oviposition showed the highest level on the 50th day. After that, the corresponding value in the eggs stored at 5$\circ$C were decreased, and the value in the eggs stored at 1$\circ$C maintained the similar level. The trehalose level was rapidly increased on 50$\sim$60th day or thereabouts, and then, it was accumulated. When the eggs stored at low temperature were tranaferred and developed the embryo at 25$\circ$C, sorbitol and glycerol were respectively changed to glycogen. Futhemore, the content of trehalose and glucose showed specific of patterns with each embryonic stage at low temperature. With the aforementioned results, the intemediary metabolism of carbohydrate was discussed with regard to embryonic development of non-diapause eggs of silkworms.

• 한국응용곤충학회지
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• 제29권4호
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• pp.230-237
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• 1990

경남 진주의 야외에서 1984년부터 1987년까지 고자리파리, Delia antoqia(Meigen)의 발생소장을 트랩을 설치하여 조사한 결과, 고자리파리는 연 3회 발생하였으며 제2화기, 성충의 발생량이 적었다. 성충의 발생최성기는 월동세대 (1화기)가 4월 중순, 제1세대 (2화기)가 6월 상순, 가을세대 (3화기)가 9월 하순-10월 상순이었다. 야외에서 충을 사육하여 우화상황을 조사한 결과, 제 1세대 용의 일부와 제2세대 용의 대부분이 하면하였다. 제1세대의 경우 야외에서 비하염용의 용기간은 22.4일 이하인 반면 하면용의 용기간은 88.5-145일로 늦게 용화한 개체일수록 짧아져서 우화최성기는 제2세대 용과 함께 9월 하순으로 일치하였다. 제1세대의 경우 5월 상순에 용화한 개체들은 43.8%가 하면용인 반면에 5월 하순 이후에 용화한 것은 86.7%이상이 하면상태이어서 용화시기가 늦을수록 그 비율이 증가하였고, 제2세대는 용화시기에 관계없이 93.3% 이상이 하 면용이 었다.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2003년도 International Symposium of Silkworm/Insect Biotechnology and Annual Meeting of Korea Society of Sericultural Science
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• pp.93-94
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• 2003

To understand the molecular mechanism of diapause determination and termination during embyogenesis of the silkworm, Bombyx mori, mRNA from maintained or activated eggs of diapause was compared using differential expression. (omitted)

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2003년도 제46회 춘계 학술연구 발표회
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• pp.49-49
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• 2003

DOPA decarboxylase (DDC), which converts DOPA to dopamine, is important for many biological event such as cuticular melanization, sclerotization and neurotransmission in insects. Recently, it has been also shown that DDC activity is correlated with pupal diapause in M. brassicae. The silkworm, Bombyx mori is a typical insect diapausing at early embryonic stage. (omitted)

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2004년도 International Conference of Sericultural and Entomological Sciences
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• pp.64-64
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• 2004

• International Journal of Industrial Entomology and Biomaterials
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• 제26권2호
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• pp.124-130
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• 2013

Bumblebees are widely used to pollinate various greenhouse crops. Among the different bumblebee species, Bombus ignitus is indigenous to Korea, China, Japan and Russia. B. ignitus undergoes one generation per year, and artificial hibernation is essential for year-round rearing of the bumblebee. Keeping the queens under low-temperature conditions for several months is an effective method for terminating their diapause and promoting colony development. In the present study, we investigated how cold temperature affects the artificial hibernation of B. ignitus queens. Under chilling temperatures of $-2.5^{\circ}C$, $0^{\circ}C$, $2.5^{\circ}C$ and $5^{\circ}C$ with constant humidity >80%, the queens stored at $2.5^{\circ}C$ exhibited the highest survival rates, which were 74.0% at one month, 67.0% at two months, 60.0% at three months, 46.0% at 4 months, 33.0% at 5 months and 24.0% at 6 months. Lower survival rates were observed at $0^{\circ}C$, $5^{\circ}C$, $7.5^{\circ}C$ and $12.5^{\circ}C$. At $2.5^{\circ}C$ the colony developmental characteristics after diapause were 1.2- to 1.5-fold greater than those when queens were stored at $5^{\circ}C$. Thus, $2.5^{\circ}C$ and 70% R.H. were the most favorable chilling temperature and humidity conditions for terminating the diapause of B. ignitus queens.

• 한국응용곤충학회지
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• 제35권3호
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• pp.205-208
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• 1996

광 및 온도가 꼬마배나무이의 겨울형성충(월동형) 발생에 미치는 영향을 조사한 결과 수원지역(북위 $37^{\circ}$16') 의 꼬마배나무이 개체군은 광길이가 14시간일 때 부터 겨울형성충이 출현하였으며 광길이가 13시간일 때 9 93% 이상의 겨울형성충이 발생하였다. 또한 $18^{\circ}C$, $25^{\circ}C$의 두 온도조건에서 겨울형성충 발생에 차이가 없었다. 꼬마배나무이의 각 발육단계별로 단일조건에 처리한 결과 1,2령기에 처리된 것은 각각 67.2, 54.4%가 겨 울형으로 되었고, 3령기는 9.3% 였으며 4령기부터는 영향을 받지 않았다. 꼬마배나무이의 평균산란수는 겨울형성충이 486.4개, 여름형성충이 387.2개 이었으며, 산란기간은 각각 34일과 24일 이었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제28권2호
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• pp.51-57
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• 2014

Bumblebees are important pollinators of crops and wildflowers. The Korean native bumblebee, Bombus ignitus, undergoes one generation per year, and induction of artificial hibernation is essential for year-round rearing of the bumblebee. Keeping queens under cold treatment conditions for several mo is an effective method for terminating their diapause and promoting colony development. In the present study, we investigated how the timing and duration of chilling affect the artificial hibernation of B. ignitus queens. In the timing assessment, cold treatment was instituted at 12 d, 40 d, or 100 d after eclosion under a constant temperature of $5^{\circ}C$ and 80% humidity. The queens that entered cold treatment at 12 d after emergence evidenced the highest survival rates: 86.7% at two mo, 73.3% at three mo, and 46.4% at 4 mo. Survival rates were reduced under storage conditions at 12 d, 40 d, and 100 d after emergence. When queens were subjected to chilling at 8 d, 12 d, or 16 d after eclosion with constant 80% humidity, the queens stored at 12 d after eclosion exhibited the highest survival rates, which were 84.6 at one mo, 25.0% at two mo, and 7.9% at three mo. In regards to the duration of the cold period, the queens that hibernated for at least two mo evidenced optimal colony development rates. The rates of oviposition, colony foundation, and progeny-queen production of queens hibernated for two mo were 60.0%, 30.0%, and 13.3%, respectively. These values were 6.0 to 13.3 times higher than those in the queens that hibernated for 15 d. Therefore, a cold period of at least 2 mo applied 12 d after emergence were found to be the most favorable conditions for diapause break in B. ignitus queens.