• Title/Summary/Keyword: development factor

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Salt-induced Differential Gene Expression in Italian Ryegrass (Lolium multiflorum Lam.) Revealed by Annealing Control Primer Based GeneFishing approach

  • Lee, Ki-Won;Lee, Sang-Hoon;Choi, Gi Jun;Ji, Hee Jung;Hwang, Tae Young;Kim, Won Ho;Rahman, Md. Atikur
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.3
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    • pp.231-236
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    • 2017
  • Salt stress is one of the most limiting factors that reduce plant growth, development and yield. However, identification of salt-inducible genes is an initial step for understanding the adaptive response of plants to salt stress. In this study, we used an annealing control primer (ACP) based GeneFishing technique to identify differentially expressed genes (DEGs) in Italian ryegrass seedlings under salt stress. Ten-day-old seedlings were exposed to 100 mM NaCl for 6 h. Using 60 ACPs, a total 8 up-regulated genes were identified and sequenced. We identified several promising genes encoding alpha-glactosidase b, light harvesting chlorophyll a/b binding protein, metallothionein-like protein 3B-like, translation factor SUI, translation initiation factor eIF1, glyceraldehyde-3-phosphate dehydrogenase 2 and elongation factor 1-alpha. These genes were mostly involved in plant development, signaling, ROS detoxification and salt acclimation. However, this study provides new molecular information of several genes to understand the salt stress response. These genes would be useful for the enhancement of salt stress tolerance in plants.

The relationship between sport talent development environment and achievement goal in sport of college athletes (대학운동선수의 스포츠영재육성환경과 스포츠성취목표의 관계)

  • Lee, Mi-Sook;Kim, Ye-Sung;Choi, Young-Jun
    • Journal of the Korean Data and Information Science Society
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    • v.23 no.3
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    • pp.475-485
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    • 2012
  • The purpose of this study was to examine the impact of the Sport Talent Development Environment on the Achievement Goal in Sport of college athletes. The Sport Talent Development Environment (Martindale et al., 2010) had seven factors, but only four factors (long-term development, lack of quality preparation, communication, lack of understanding) were used after exploratory factor analysis. The Achievement Goals in Sport (Wang et al., 2007) was composed of four factors (mastery approach, mastery avoidance, performance mastery, performance avoidance). The results showed that 1)long-term development factor and communication factor of male athletes were significantly higher than female athletes while lack of understanding factor of female athletes were significantly higher than male athletes, 2)mastery avoidance factor of female athletes was significantly higher than male athletes, and 3)factors of development environment accounted for 27.8% to 34.5%-male athletes and for 13.2% to 22.7%-female athletes variance in the four achievement goals. Therefore, it is important for coaches and sport administrators to take these results into consideration when designing an effective talent development program.

Effects of Overexpression of Brassica rapa GROWTH-REGULATING FACTOR Genes on B. napus Organ Size (배추 GROWTH-REGULATING FACTOR 유전자 발현이 유채 기관크기에 미치는 영향)

  • Hong, Joon Ki;Suh, Eun Jung;Lee, Seung-Bum;Yoon, Hye-Jin;Lee, Yeon-Hee
    • Korean Journal of Breeding Science
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    • v.50 no.4
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    • pp.378-386
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    • 2018
  • GROWTH-REGULATING FACTOR (GRF) genes encode plant-specific transcription factors and play critical roles in regulating the growth and development of lateral organs. In order to explore the agricultural potential of Brassica rapa GRF genes (BrGRFs), we constructed two BrGRF-overexpressing B. napus plants (BrGRF3-1OX and -9OX). BrGRF3-1OX and -9OX developed larger cotyledons, leaves, and seeds than the wild type. The increased organs' sizes were due to increases in cell number, but not due to cell size alterations. RT-PCR analysis revealed that BrGRFs regulated the expression of a wide range of genes that are involved in gibberellin-, auxin-, cell division-related growth processes. Taken together, our data indicate that BrGRFs act as positive regulators of B. napus growth, thus raising the possibility that they may serve as a useful genetic source for crop improvement with respect to organ size and seed production.

APPLICATION OF WHOLE BODY COUNTER TO NEUTRON DOSE ASSESSMENT IN CRITICALITY ACCIDENTS

  • Kurihara, O.;Tsujimura, N.;Takasaki, K.;Momose, T.;Maruo, Y.
    • Journal of Radiation Protection and Research
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    • v.26 no.3
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    • pp.249-253
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    • 2001
  • Neutron dose assessment in criticality accidents using Whole Body Counter (WBC) was proved to be an effective method as rapid neutron dose estimation at the JCO criticality accident in Tokai-mura. The 1.36MeV gamma-ray of $^{24}Na$ in a body can be detected easily by a germanium detector. The Minimum Detectable Activity (MDA) of $^{24}Na$ is approximately 50Bq for 10miniute measurement by the germanium-type whole body counter at JNC Tokai Works. Neutron energy spectra at the typical shielding conditions in criticality accidents were calculated and the conversion factor, whole body activity-to-organ mass weighted neutron absorbed dose, corresponding to each condition were determined. The conversion factor for uncollied fission spectrum is 7.7 $[(Bq^{24}Na/g^{23}Na)/mGy]$.

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An Analysis of the Regional Characteristics in Agropolitan Cities for Sustainable Development (도농통합시의 지속가능한 개발을 위한 지역특성 분석)

  • Park, Kyung-Hun;Jung, Sung-Kwan;Choi, Won-Myeung
    • Journal of the Korean Association of Geographic Information Studies
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    • v.3 no.2
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    • pp.37-47
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    • 2000
  • Since 1995, agropolitan cities have been created, in order to pursue the balanced development between urban cities and its surrounding rural counties. However, the inequality of regional level that was caused by indiscreet development has become the ever-serious problems recently. Therefore, this study aims to analyze regional characteristics and patterns for setting up the sustainable spatial planning. Firstly, the regional characteristics were summarized by five factors; development-oriented factor, agricultural factor, living environmental factor, rice growing, fruit gardening factor. The regional patterns were classified with five patterns using cluster analysis; orchard farming, farming of medium and small size, small stagnation, under urbanization, mixed urban-rural properties, and industry of southeastern seashore. Accuracy of the results by discrimination analysis showed that pattern II, V, and VI were confidence level of 100%, but the others had nearly 90% confidence level.

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Assessment of N2O Emission Factor of Autumn Chinese Cabbage Fields at Three Different Geographical Location in South Korea

  • Kim, Gun-Yeob;Park, Woo-Kyun;Jeong, Hyun-Cheol;Lee, Sun-il;Kim, Pil-Joo;Seo, Young-Ho;Na, Un-sung
    • Korean Journal of Soil Science and Fertilizer
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    • v.48 no.3
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    • pp.163-169
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    • 2015
  • The level of nitrous oxide ($N_2O$), a long-lived greenhouse gas, in atmosphere has increased mainly due to anthropogenic sources, especially application of nitrogen fertilizers. Quantifying $N_2O$ emission in the agricultural field is essential to develop national inventories of greenhouse gases (GHGs) emission. The objective of this study was to develop an emission factor to estimate the direct $N_2O$ emission from an agricultural field cultivated with the Chinese cabbage during autumn season in 2010-2012. Emission factor of $N_2O$ calculated over three years experiment using accumulated $N_2O$ emission, nitrogen fertilization rate, and background $N_2O$ emission was $0.0058{\pm}0.00254kg\;N_2O-N\;kg^{-1}\;N$. More extensive studies need to be conducted to develop $N_2O$ emission factors for other upland crops in the various regions of Korea because $N_2O$ emission is influenced by many factors including climate characteristics, soil properties, and agricultural practices as well as crop species.

AKT-targeted anti-inflammatory activity of Panax ginseng calyx ethanolic extract

  • Han, Sang Yun;Kim, Juewon;Kim, Eunji;Kim, Su Hwan;Seo, Dae Bang;Kim, Jong-Hoon;Shin, Song Seok;Cho, Jae Youl
    • Journal of Ginseng Research
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    • v.42 no.4
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    • pp.496-503
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    • 2018
  • Background: Korean ginseng (Panax ginseng) plays an anti-inflammatory role in a variety of inflammatory diseases such as gastritis, hepatitis, and colitis. However, inflammation-regulatory activity of the calyx of the P. ginseng berry has not been thoroughly evaluated. To understand whether the calyx portion of the P. ginseng berry is able to ameliorate inflammatory processes, an ethanolic extract of P. ginseng berry calyx (Pg-C-EE) was prepared, and lipopolysaccharide-activated macrophages and HEK293 cells transfected with inflammation-regulatory proteins were used to test the anti-inflammatory action of Pg-C-EE. Methods: The ginsenoside contents of Pg-C-EE were analyzed by HPLC. Suppressive activity of Pg-C-EE on NO production, inflammatory gene expression, transcriptional activation, and inflammation signaling events were examined using the Griess assay, reverse transcription-polymerization chain reaction, luciferase activity reporter gene assay, and immunoblotting analysis. Results: Pg-C-EE reduced NO production and diminished mRNA expression of inflammatory genes such as cyclooxygenase-2, inducible NO synthase, and tumor necrosis factor-${\alpha}$ in a dose-dependent manner. This extract suppressed luciferase activity induced only by nuclear factor-${\kappa}B$. Interestingly, immunoblotting analysis results demonstrated that Pg-C-EE reduced the activities of protein kinase B (AKT)1 and AKT2. Conclusion: These results suggest that Pg-C-EE may have nuclear-factor-${\kappa}B$-targeted anti-inflammatory properties through suppression of AKT. The calyx of the P. ginseng berry is an underused part of the ginseng plant, and development of calyx-derived extracts may be useful for treatment of inflammatory diseases.

LAMINAR FLOW IN THE ENTRANCE REGION OF HELICAL TUBES FOR UNIFORM INLET VELOCITY CONDITIONS (균일입구유속 조건의 나선관 입구영역의 층류 유동)

  • Kim, Y.I.;Park, J.H.
    • Journal of computational fluids engineering
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    • v.13 no.1
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    • pp.21-27
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    • 2008
  • A numerical study for laminar flow in the entrance region of helical tubes for uniform inlet velocity conditions is carried out by means of the finite volume method to investigate the effects of Reynolds number, pitch and curvature ratio on the flow development. This results cover a curvature ratio range of 1/10$\sim$1/320, a pitch range of 0.0$\sim$3.2, and a Reynolds number range of 125$\sim$2000. It has been found that the curvature ratio does significantly effect on the angle of flow development, but the pitch and Reynolds number do not. The characteristic angle $\phi_c(=\phi/\sqrt{\delta})$, or the non-dimensional length $\overline{l}(=l\sqrt{\delta}cos(atan\lambda)/d)$ can be used to represent the flow development for uniform inlet velocity conditions. In uniform inlet velocity conditions, the growth of boundary layer delays the flow development attributed to centrifugal force, and in which conditions the amplitude of flow oscillations is smaller than that in parabolic inlet velocity conditions. If the pitch increases or if the curvature ratio or Reynolds number decreases, the minimum friction factor and the fully developed average friction factor normalized with the friction factor of a straight tube and the flow oscillations decrease.

A Study on Evaluation of Culture and Leisure Resources of Rural Areas (농촌의 문화여가자원 평가를 위한 척도 개발 연구)

  • Hwang, Jeong-Im;Choi, Yoon-Ji;Youn, Min-Hye
    • Journal of Agricultural Extension & Community Development
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    • v.22 no.3
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    • pp.355-363
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    • 2015
  • The purpose of this study is to develop a culture and leisure resources scale and examine the validity of the scale. The culture and Leisure resources scale was developed and identified its validity by exploratory factor analysis, confirmatory factor analysis, and correlation analysis. The culture and leisure resources scale consisted of four factors, which were 'personal resources', 'support staff resources', 'facility resources', and 'program resources'. Those four factors indicated the reasonable fit indices from confirmatory factor analysis. In addition, this scale showed convergent validity with the satisfaction of cultural and leisure environment and the satisfaction of one's cultural and leisure life. The result of scale score comparison between rural and urban areas showed that culture and leisure resources in rural areas were less abundant than those in urban areas. Furthermore, while the variables which affected the satisfaction of urban residents' were mainly 'personal resources', the variables in case of rural residents' were mainly 'program resources' and 'facility Resources'. The culture and leisure resources scale can be used for policymakers practicably to evaluate regional level of culture and leisure resources, to compare between regions and to find policy priorities for improving the quality of leisure, especially for disadvantaged areas with less resources.

A Study on the Impact of Management's Strategic Leadership and Management Strategy on Organizational Performance: Focusing on Small and Medium Venture Companies

  • Kim, Moon Jun
    • International journal of advanced smart convergence
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    • v.9 no.1
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    • pp.121-131
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    • 2020
  • We study empirically analyzes the relationship between the leadership styles and management strategies of executives perceived by members of small and medium venture companies through organizational performance through SPSS 24.0. The empirical results are as follows. First, the hypothesis that the strategic leadership of the one-level management team had a significant influence on the management strategy showed that strategic leadership (strategic direction, strategic control, maintaining effective organizational culture, ethical management, human resource development, competency development) The relationship between positive cost, strategy of differentiation, and strategy of concentration was positive. Second, the hypothesis 2 management strategy (cost advantage strategy, differentiation strategy, centralization strategy) was statistically significant for both organizational performance (financial performance and non-financial performance). Therefore, management strategy implemented by management acts as a factor to improve organizational performance. Therefore, the execution ability of management strategy should be strengthened. Third, hypothesis 3 (Strategic Direction, Strategic Control, Maintaining Effective Organizational Culture, Ethical Management, Human Resource Development, Competency Development) could be identified as an important role factor for financial and non-financial performance. The organizational performance of SMEs has been a key factor in the strategic leadership and management strategy implemented by management. Therefore, the establishment and implementation of various practical measures to upgrade this were continuously required.