• Microbiology and Biotechnology Letters
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• v.10 no.3
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• pp.205-209
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• 1982

The enzyme-linked immunosorbent assay using the so-called "double-sandwich"technique was applied to determine Clostridium botulinum type F toxin. Polystyrene tubes were coated with horse anti-type F toxin serum and then toxin sample was added. The tubes were subsequently treated with rabbit anti-type F toxin IgG and sheep anti-rabbit serum IgG-horseradish peroxidase conjugate. By this technique, about 10 mouse intraperitoneal 50% lethal doses (ip LD/50/) of type F toxin could be detected. Low back-ground reading was achieved with the use of phosphate-buffered saline containing 0.05% Tween 20 and 1% bovine serum albumin as diluents of rabbit IgG and conjugate. Addition of EDTA in the diluents of toxin increased ELISA extinction value significantly. No cross-reaction was observed with botulinum type A and B toxin, but type E toxin gave sleight cross-reaction.

• Journal of information and communication convergence engineering
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• v.10 no.4
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• pp.378-383
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• 2012

This paper presents the implementation of an automatic road sign recognizer for an intelligent transport system. In this system, lists of road signs are processed with actions such as line segmentation, single sign segmentation, and storing an artificial sign in the database. The process of taking the video stream and extracting the road sign and storing in the database is called the road sign recognition. This paper presents a study on recognizing traffic sign patterns using a segmentation technique for the efficiency and the speed of the system. The image is converted from one scale to another scale such as RGB to grayscale or grayscale to binary. The images are pre-processed with several image processing techniques, such as threshold techniques, Gaussian filters, Canny edge detection, and the contour technique.

• Journal of Korea Multimedia Society
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• v.16 no.12
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• pp.1439-1445
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• 2013

We present a Taekwondo training system using a hybrid sensing technique of a body sensor and a visual sensor. Using a body sensor (accelerometer), rotational and inertial motion data are captured which are important for Taekwondo motion detection and evaluation. A visual sensor (camera) captures and records the sequential images of the performance. Motion chunk is proposed to structuralize Taekwondo motions and design HMM (Hidden Markov Model) for motion recognition. Trainees can evaluates their trial motions numerically by computing the distance to the standard motion performed by a trainer. For motion training video, the real-time video images captured by a camera is overlayed with a visualized body sensor data so that users can see how the rotational and inertial motion data flow.

• Proceedings of the KIEE Conference
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• 2006.10c
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• pp.300-302
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• 2006

The structured lighting technique is a very accurate depth measurement method. One problem in this technique is that the laser light is invisible very often partly or as a whole caused from occlusion, light absorbtion, and reflection on the surface. If there is no laser light detected on a row of the image frame, the depth could not be computed at the point on the row. In this paper, a Dynamic Programing-based efficient laser line tracking algorithm is proposed to find the most likely line of the laser light utilizing all the possible information of the laser light trace. The performance of the algorithm is shown.

• Proceedings of the KSR Conference
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• 2004.06a
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• pp.1422-1424
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• 2004

Various deterioration diagnostic techniques and devices are suggested, and most of which measure leakage current components as an indicator of arrester ageing. However, the techniques based on the magnitude of leakage current measure simply RMS or peak value of leakage current components and do not provide detailed information needed in the diagnosis. In this study, we found that the phase vs. wave height of total leakage current is changed or a new wave height is produced with arrester ageing. To complete the new technique, we designed an arrester diagnostic device which is composed of a current detection circuit, an optical transmission circuit, and an analysis program. After measurement of the total leakage current, magnitudes, phase vs. wave height, and harmonics of the leakage current components are analyzed by the microprocessor based device. From the experimental results, we confirmed that the device can measure most parameters needed for the arrester diagnostics and analyze an initial deterioration state.

• Tuberculosis and Respiratory Diseases
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• v.39 no.1
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• pp.1-6
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• 1992

The diagnosis of tuberculosis is usually established using staining and culturing techniques. Fluorescent stains have improved the sensitivity of direct microscopy. Improved culture media coupled with radiometric means of detecting early mycobacterial growth have shortened the time needed for cultural diagnosis. Rapid immunodiagnostic techniques based on the detection of mycobacterial antigen or of antibodies to theses antigens have not, however, come into widespread clinical use. The DNA or RNA hybridization tests with labeled specific probes which have been described so far are not sensitive enough to be used for clinical speicimens without prior culturing. The advent of the polymerase chain reaction (PCR) has opened new possibilities for diagnosis of microbial infections. This technique has already been applied to a number of microorganisms. In the field of mycobacteria the PCR has been used to identify and to detect DNAs extracted from various mycobacteria. However, despite the extraordinary enthusiasm surrounding this technique and the considerable investiment, PCR has not emerged from the developmental "trenches" in the passed several years. It may be a considerable lenth of time before clinical microbiology laboratories become PCR playgrounds because many details remain to be worked out.

• Journal of Ocean Engineering and Technology
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• v.19 no.4 s.65
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• pp.21-27
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• 2005

Large welded structures, including ships and offshore structures, are normally in operation under cyclic fatigue loadings. These structures include many geometric discontinuities, as well as material discontinuities due to weld joints. The fatigue strength at these hot spots is very important for the structural performance. In the past, various Non Destructive Evaluation (NDE) techniques have been developed to detect fatigue cracks and to estimate their location and size. However, an important limitation of most of the existing NDE methods is that they are off line; the normal operation of the structure has to be interrupted, and the device often has to be disassembled. This study explores the development of a structural health monitoring system, with a special interest in applying the technique to welded structural members in ship and offshore structures. In particular, the impedance based structural health monitoring technique that employs the coupling effect of piezoceramic (PZT) materials and structures is investigated.

• Journal of the Korean Institute of Intelligent Systems
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• v.23 no.1
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• pp.18-23
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• 2013

In this paper we propose an image enhancement technique based on histogram specification method over local overlapping regions referred as Local Histogram Specification. First, both reference and original images are splitted into local regions that each overlaps half of its adjacent regions and general histogram specification method is used between corresponding local regions of reference and original image. However it produces noticeable boundary effects. Linear weighted image blending method is used to reduce this effect in order to make seamless image and we also proposed new technique dealing with over-enhanced contrast areas. We satisfied with our experimental results that showed better enhancement accuracy and less noise amplifications compared to other well-known image enhancement methods. We conclude that the proposed method is well suited for motion detection systems as a responsible part to overcome sudden illumination changes.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.6 no.8
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• pp.1319-1324
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• 2002

In this paper, we present a new technique for extraction of moving objects in moving image sequence. The detection method of change regions is based on spatial gradient difference and temporal difference. Moving objects are extracted by motion information and prediction error of each region. In the simulation results, the proposed technique shows good performance.

• Journal of information and communication convergence engineering
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• v.13 no.3
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• pp.189-196
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• 2015

In this paper, we present a rotating auto-zeroing offset cancellation technique, which can improve the performance of touch screen sensing circuits. Our target touch screen detection method employs multiple continuous sine waves to achieve a high speed for large touch screens. While conventional auto-zeroing schemes cannot handle such continuous signals properly, the proposed scheme does not suffer from switching noise and provides effective offset cancellation for continuous signals. Experimental results show that the proposed technique improves the signal-to-noise ratio by 14 dB compared to a conventional offset cancellation scheme. For the realistic simulation results, we used Cadence SPECTRE with an accurate TSP model and noise source. We also applied an asymmetric device size (10% MOS size mismatch) to the OP Amp design in order to measure the effectiveness of offset cancellation. We implemented the proposed circuit as part of a touch screen controller system-on-chip by using a Magnachip/SK Hynix 0.18-µm complementary metal-oxide semiconductor (CMOS) process.