• Title/Summary/Keyword: detection

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Screening of Deoxynivalenol Producing Fungi from Greenhouse Horticulture by Enzyme Amplification System Immunoassay (Enzyme Amplification System Immunoassay에 의한 시설원예산물의 Deoxynivalenol 생성곰팡이의 검색)

  • Park, Mi-Ja;Park, Jung-Hyun;Chung, Duck-Hwa
    • Korean Journal of Food Science and Technology
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    • v.32 no.2
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    • pp.439-443
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    • 2000
  • In order to evaluate the safety of greenhouse horticulture products in Korea, we carried out this work by screening of Fusarium species, which produce deoxynivalenol (DON) from greenhouse horticulture in Western Gyeongnam and Northern Gyeongbuk, Korea. For this study, high sensitive enzyme-linked immunosorbent assay, ALP/NADP method, was applied to detection of DON by enzyme amplification system. From 192 samples of greenhouse horticulture soil and its products, 103 isolates of Fusarium species were obtained. The isolates were cultured at 28C for 15 days and the cultured mediums were extracted by ethyl acetate. The production of DON was verified by thin layer chromatography (TLC). As the results of TLC, 8 strains were identified as DON producing strain. We screened potential producers of DON by ALP/NADP. The levels of DON production were shown from 0.007 to 1.21 g/ml of YES medium. The maximum DON producing strain No. 32-D-3 was isolated from soil in Namhae, Korea. In conclusion, the above results indicate that DON producing fungi contaminated greenhouse horticulture products in Korea. Therefore, further studies are required to accumulate more detailed data about the contamination of DON in various cereals.

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A Method for Analysis of Styrene Dimer and Trimer in Foods and Containers (식품 및 용기 중 스티렌 다이머 및 트리머의 분석법)

  • Sung, Jun-Hyun;Kwon, Ki-Sung;Lee, Kwang-Ho
    • Korean Journal of Food Science and Technology
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    • v.32 no.6
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    • pp.1234-1243
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    • 2000
  • A method for quantitative determination of styrene dimer and trimer, which are suspected as endocrine disruptors, in foods and containers was studied. For residual contents of styrene dimer and trimer in two-kinds of containers, which contained instant noodle and yogurt, sample pieces were completely dissolved in tetrahydrofuran. The polymer was precipitated with n-hexane, a portion of supernatant was concentrated for analysis. A sensitive method was also optimized for the quantification of styrene dimer and trimer in foods such as instant cup noodle and yogurt by using GC/MS. Limits of Detection were about 3.2-87.0 ppb for styrene dimers and trimers. The highest recovery was obtained by extraction with acetone/hexane(1:1), followed by florisil clean-up. The levels of styrene dimer and trimer migrated by food simulants were much higher in heptane.

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Evaluation of Field Application for the mix properties of the thixotropic grout (가소성그라우트의 배합특성 및 현장 적용성 평가)

  • Kim, Hak-Moon;Jang, Kyung-Jun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.9
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    • pp.4223-4238
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    • 2011
  • The advantages of grouting are the simple instrument, the simple procedure of practice and the simple verification of grouting result. but the more grouting practice there is, the more damages of environment from grouting practice and grouting materials there are. so, the grouting materials and methods with the character of environment friendly are introduced in construction field, recently. This paper is to study of the physical characteristics that has thixotropic character and that consists of inorganic and polycarboxylate co-polymer. In study, various testing methods are performed such as a viscosity, a thixotropy, a compressive strength, a heavy metal detection and pH measurement in lab test and a low pressure injection test and a high pressure injection test in field with different soil type. As a result, a optimum mix ratio is proposed by analyzing the result of lab test. the field applicability is verified by checking a injection pressure, a grout volume and a hardened body of grout by excavating the practice site.

Segmentation and Volume Calculation through the Analysis of Blurred Gray Value from the Brain MRI (뇌의 MR 영상에서 번짐 현상의 명암 값 분석을 통한 백질과 회백질의 추출 및 체적 산출)

  • Sung, Yun-Chang;Yoo, Seung-Wha;Song, Chang-Jun;Park, Jong-Won
    • Journal of KIISE:Software and Applications
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    • v.27 no.8
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    • pp.815-826
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    • 2000
  • This study is for the segmentation and volume calculation of the white matter and gray matter from brain MRI. In general, the volume of white and gray matter is reduced by contraction of each components in the case of mental retardation which are Alzheimer's disease and Down's syndrome. As results, it is useful for diagnostic and early detection for various mental retardation through the tracing of variation for its volume from the brain MRI. But, until now, it was very difficult to calculate the partial volume of each components existing in some thickness, because MR image was represented by single gray value after scanning by MR scanner. Accordingly, new segmentation algorithm proposed in this paper is to calculate the partial volume of the white and gray matter existing in some thickness through the analysis of the blurred gray value, and is to determine the threshold for segmentation of white and gray matter, and is to calculate the volume of each segmented component. And finally, proposed algorithm was applied the models which was created manually, and then acquired results was compared with that of original model.

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Effect of maternal gene expression on porcine oocytes in vitro maturation (돼지 미성숙 난자 모계 유전자 발현이 체외성숙에 미치는 영향)

  • Lee, Jae-Dal
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.8
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    • pp.3532-3536
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    • 2012
  • Understanding of the maternal transcriptome increased to elucidate the underlying molecular mechanism of normal oocyte maturation, which depends on a precise sequence of changes in maternal genes expression. Previous reports that the translational potential of a maternal mRNA is generally determined by the length of the poly(A) tail, and deadenylation is usually the first sign of mRNA degradation. However, in vitro cultured system has the underlying molecular mechanisms remain unclear. We determined whether the role of molecular basis, four important maternal genes, C-mos, cyclin-B1 (regulatory subunit of MPF), BMP15 and GDF9, were selected for detection of their precise mRNA expression patterns by real-time PCR and for determination of their polyadenylation status by poly(A) tail PCR during oocyte maturation. In the present study. the abnormal expression of maternal mRNAs prior to zygotic genome activation, which results in suppression of the corresponding protein level, may be responsible for, at least in part, a profound defect in further embryonic development. Reasonable expression of maternal gene is crucial for proper oocyte maturation and further embryonic development.

Evaluation of Depth Image of IR Range Sensor with Face Recognition Algorithms (적외선 거리 센서 깊이이미지를 이용한 얼굴 인식 알고리즘 평가)

  • Kwon, Ki-Hyeon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.8
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    • pp.3666-3671
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    • 2012
  • We evaluate the face detection and recognition of depth image that is obtained by infrared range sensor. and Face recognition was usually focused on accuracy aspect but it is not enough to evaluate the performance in testing for real world application. In this paper, we evaluate the overall performance like accuracy, training, test speed and memory use for the well known face recognition algorithm like PCA, LDA, ICA and SVM. This experiment evaluate the good results of depth and colored depth image compatible with the colored image although the file size of depth and colored depth image is 30%~40% less than the colored image. Whereas, LDA got the good accuracy performance next to the SVM and also shows the good performance in speed and the amount of memory.

Developing of Text Plagiarism Detection Model using Korean Corpus Data (한글 말뭉치를 이용한 한글 표절 탐색 모델 개발)

  • Ryu, Chang-Keon;Kim, Hyong-Jun;Cho, Hwan-Gue
    • Journal of KIISE:Computing Practices and Letters
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    • v.14 no.2
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    • pp.231-235
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    • 2008
  • Recently we witnessed a few scandals on plagiarism among academic paper and novels. Plagiarism on documents is getting worse more frequently. Although plagiarism on English had been studied so long time, we hardly find the systematic and complete studies on plagiarisms in Korean documents. Since the linguistic features of Korean are quite different from those of English, we cannot apply the English-based method to Korean documents directly. In this paper, we propose a new plagiarism detecting method for Korean, and we throughly tested our algorithm with one benchmark Korean text corpus. The proposed method is based on "k-mer" and "local alignment" which locates the region of plagiarized document pairs fast and accurately. Using a Korean corpus which contains more than 10 million words, we establish a probability model (or local alignment score (random similarity by chance). The experiment has shown that our system was quite successful to detect the plagiarized documents.

Dynamic Rectangle Zone-based Collaboration Mechanism for Continuous Object Tracking in Wireless Sensor Networks (센서 네트워크에서 연속적인 개체 추적을 위한 동적 직사각형 영역 기반 협동 메커니즘)

  • Park, Bo-Mi;Lee, Eui-Sin;Kim, Tae-Hee;Park, Ho-Sung;Lee, Jeong-Cheol;Kim, Sang-Ha
    • Journal of KIISE:Computing Practices and Letters
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    • v.15 no.8
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    • pp.591-595
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    • 2009
  • Most existing routing protocols for object detection and tracking in wireless sensor networks concentrate on finding ways to detect and track one and more individual objects, e.g. people, animals, and vehicles, but they do not be interested in detecting and tracking of continuous objects, e.g., poison gas and biochemical. Such continuous objects have quite different properties from the individual objects since the continuous objects are continuously distributed across a region and usually occupy a large area, Thus, the continuous objects could be detected by a number of sensor nodes so that sensing data are redundant and highly correlated. Therefore, an efficient data collection and report scheme for collecting and locally aggregating sensing data is needed, In this paper, we propose the Continuous Object Tracking Mechanism based on Dynamic Rectangle Zone for detecting, tracking, and monitoring the continuous objects taking into account their properties.

Improment of Diesel Combustion using multiple injection under Cold Start Condition (냉시동 조건에서 디젤 연소 특성 및 연소 개선에 대한 연구)

  • Lee, Haeng-Soo;Lee, Jin-Woo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.4
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    • pp.711-717
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    • 2017
  • Startability and harmful emissions are the main issues in diesel engine development under cold conditions. The characteristics of combustion with multiple injection were investigated under cold start conditions. For quantitative analysis, the in-chamber pressure profile was measured and combustion visualization using direct imaging was accomplished. With multiple injection, the peak in-chamber pressure and heat release rate were increased compared to single injection. In addition, the period of flame luminosity detection was shortened using multiple injection. Combustion by main injection was improved with an increase in heat released by pilot combustion when the pilot injection quantity was increased. Finally, an increase in injection pressure also showed the possibility of combustion improvement. On the other hand, an increase of in the pilot injection quantity and injection pressure can cause an increase in harmful emissions, such as HC and CO due to wall wetting. Therefore, more sensitive calibration will be needed when applying a multiple injection strategy under cold start conditions.

Video Indexing and Retrieval of MPEG Video using Motion and DCT Coefficients in Compressed Domain (움직임과 DCT 계수를 이용한 압축영역에서 MPEG 비디오의 인덱싱과 검색)

  • 박한엽;최연성;김무영;강진석;장경훈;송왕철;김장형
    • Journal of Korea Multimedia Society
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    • v.3 no.2
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    • pp.121-132
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    • 2000
  • Most of video indexing applications depend on fast and efficient archiving, browsing, retrieval techniques. A number of techniques have been approached about only pixel domain analysis until now. Those approaches brought about the costly overhead of decompressing because the most of multimedia data is typically stored in compressed format. But with a compressed video data, if we can analyze the compressed data directly. then we avoid the costly overhead such as in pixel domain. In this paper, we analyze the information of compressed video stream directly, and then extract the available features for video indexing. We have derived the technique for cut detection using these features, and the stream is divided into shots. Also we propose a new brief key frame selection technique and an efficient video indexing method using the spatial informations(DT coefficients) and also the temporal informations(motion vectors).

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