• Journal of Microbiology and Biotechnology
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• 제33권11호
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• pp.1467-1474
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• 2023

Due to the continuous increase in patients with androgenetic alopecia (AGA) and psychological disorders such as depression and anxiety, the demand for hair loss treatment and effective hair growth materials has increased. Terminalia bellirica (Gaertn.) Roxb. (TBE) reportedly exerts anti-inflammatory, hepatoprotective, and antidiabetic effects, among others, but its effects on testosterone (TS)-inhibited hair growth remains unclear. In this study, we evaluated the effects of TBE on TS-induced hair growth regression in human follicle dermal papilla cells (HFDPCs) and C57BL/6 mice. Oral administration of TBE increased TS-induced hair growth retardation. Interestingly, effects were greater when compared with finasteride, a commercial hair loss treatment product. Histological analyses revealed that oral TBE administration increased hair follicles in the dorsal skin of C57BL/6 mice. Additionally, western blotting and immunofluorescence showed that oral TBE administration recovered the TS-induced inhibition of cyclin D1, proliferating cell nuclear antigen (PCNA), and Ki67 expression in vivo. Using in vitro proliferation assays, TBE promoted HFDPC growth, which was suppressed by TS treatment. Thus, TBE may be a promising nutraceutical for hair health as it promoted hair growth in AGA-like in vitro and in vivo models.

• 생약학회지
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• 제52권4호
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• pp.234-241
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• 2021

Dermal papilla cells (DPCs) are present throughout the hair cycle and play an essential role in hair cycle and hair growth. In this study, we investigated the effect of Carex dispalata on the activation of anagen pathway in DPCs. C. dispalata extract increased the proliferation of DPCs and induced changes in the levels of cell cycle-related proteins. To elucidate the mechanism by which C. dispalata extract stimulates the anagen pathway related to the proliferation of DPCs, we evaluated the effect of C. dispalata extract on the activation of Akt signaling. The increase in the level of phospho-Akt by C. dispalata extract was inhibited by PI3K inhibitor (wortmannin). Wortmannin reduced the effects of C. dispalata extract on the levels of cell cycle-related proteins and proliferation of DPCs. C. dispalata extract increased the levels of Wnt/β-catenin proteins. Wnt/β-catenin inhibitor (XAV939) inhibited changes in cell cycle, cell cycle-related proteins, Wnt/β-catenin proteins, and proliferation induced by C. dispalata extract. C. dispalata extract increased the level of autophagy protein (LC3I/II), and this change was inhibited by XAV939. These results suggest that C. dispalata extract can activate PI3K/Akt, Wnt/β-catenin, and autophagy pathways in DPCs to induce cell proliferation, and thereby promote hair growth phase.

• 대한화장품학회지
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• 제49권1호
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• pp.75-85
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• 2023

본 연구에서는 7 개의 아미노산으로 이루어진 헵타펩타이드의 Lgr5 binding에 따른 인체 모낭 구성 세포의 활성에 대한 영향을 확인하였다. 표면 플라즈몬 공명(surface plasmon resonance, SPR) 시스템을 이용하여 헵타펩타이드가 Lgr5에 결합하는 것을 확인하였다. 인체 모유두세포(human hair follicle dermal papilla cell, HHFDPC)에 헵타펩타이드를 처리한 결과, 농도 의존적인 세포 증식이 나타났으며 β-catenin의 세포 내핵 이동 및 하위 유전자인 LEF1, Cyclin-D1, c-Myc의 발현 증가가 관찰되었다. 그리고 세포 증식 기전 관련 인자인 Akt와 ERK의 인산화 수준이 증가되었으며, 성장인자인 hepatocyte growth factor (HGF), keratinocyte growth factor (KGF), vascular endothelial growth factor (VEGF) 발현이 유도되었다. 또한 인체 모모세포(human hair germinal matrix cell, HHGMC)의 분화 관련 전사 인자와 인체 외모근초세포(human hair outer root sheath cell, HHORSC)의 분화 표지 인자들도 헵타펩타이드 처리 시 높은 발현율을 보였다. 추가적으로 우리는 헵타펩타이드의 인체 모낭줄기세포(human hair follicle stem cell, HHFSC) 분화에 대한 영향을 조사하였다. 그 결과, HHFSC 표지인자들의 mRNA와 단백질 수준이 감소하였고 반면에 분화 표지인자들은 증가하였다. 상기의 결과들은 헵타펩타이드가 인체 모낭 구성 세포에서 Wnt/β-catenin 경로를 촉진시켜 증식 또는 분화를 유도할 수 있음을 보여준다. 이를 토대로 종합해 볼 때, 본 연구의 헵타펩타이드는 모발 성장을 유도하고 탈모 개선에 도움을 줄 수 있는 기능성 원료로 사용될 수 있을 것으로 보인다.

• 대한한의학회지
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• 제43권1호
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• pp.18-32
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• 2022

Objectives: This study aimed to examine the safety, effects on proliferation of hair papilla cells, and anti-inflammatory and antioxidant mechanisms of Artemisia sieversiana Ehrh. ex Willd. (AS) extract. Methods: Safety tests through purity testing, acute toxicity tests, and repeated toxicity tests were performed using AS extract (ASE) which had been dried for over two years. Cell culture and proliferation tests were conducted; VEGF (vascular endothelial growth factor), bFGF (basic fibroblast growth factor), and EGF (epidermal growth factor) and protein expression analyses were performed for mechanistic evaluation; and inhibitory effects of ASE on the RNA expression of testosterone, 5𝛼-reductase, and aromatase was assessed. The anti-inflammatory and antioxidant efficacy of ASE was confirmed by measuring the levels of nitric oxide, inflammatory mediators (TNF-𝛼 and PGE2), inflammatory cytokines (IL-1𝛽, IL-6, and IL-8), and chemokine MCP-1. Results: The safety of ASE was confirmed. The mechanism of cell proliferation in human hair follicle dermal papilla cells involved the promotion of VEGF, bFGF, and EGF expression. ASE decreased mRNA expression of testosterone, 5𝛼-reductase, and aromatase-1 in a concentration-dependent manner. PGE2 and TNF-𝛼 production by inflammatory mediators was also significantly decreased in a concentration-dependent manner, and inflammatory cytokine and chemokine expression was inhibited. Conclusions: ASE is suggested to promote papillary cell growth at the cellular level, to suppress expression of various enzymes involved in hair cycle and cell death, and to inhibit hair loss through anti-androgen, anti-inflammatory, and antioxidant effects.

• Journal of Applied Biological Chemistry
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• 제65권4호
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• pp.269-275
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• 2022

자작나무(Betula platyphylla) 수피 추출물에는 생리활성을 보일 수 있는 다수의 terpenoids 물질이 포함되어 있고 염증반응을 완화시키는 치료제로 사용되어 왔다. 본 연구는 탈모예방 효과를 보일 수 있는 천연 물질을 확보하기 위해 자작나무를 여러 용매에서 추출 및 분리 정제하여 몇몇 분획을 획득하였다. 이를 사용하여 사람 모낭 모유두세포(HDP)에 대한 세포 독성 및 성장촉진 효과 그리고 β-catenin, VEGF, IGF1, Cyclin D의 발현변화와 5-α-reductase activity에 미치는 영향을 평가하였다. Ethyl acetate를 이용하여 분리한 4개의 분획 중 H3-2 분획은 알려진 유효물질인 ETB, LPO와 동등수준의 모낭 모유두세포(HDP)에 대한 성장 촉진 효과를 보였고 성장에 관여하는 주요인자들의 발현을 높이는 효과를 보였다. 또한, 5-α-reductase activity에 ETB, LPO가 큰 영향을 보이지 못한 반면 최종분획물인 H3은 5-α-reductase activity 감소활성이 있음을 확인할 수 있었다.

• 한방안이비인후피부과학회지
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• 제22권1호
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• pp.62-75
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• 2009

Objective : The purpose of this study is to examine the effects of Gamisipjeontang-gamibang(GT) on an experimental bum elicitation. Method : The absorbance of the photo cell mixed with GT at the Abs 560 nm was measured after irradiation for 1 min. In order to know the antioxidant effect on skin cell of mice after burn elicitation, superoxide dismutase(SOD) activity was measured. Also, in order to know effects on the skin regeneration in the burned mice, we counted the eosinophil in blood from animals via cardiac puncture and observed the histological structure in the epidermal basal layer and the dermal section on the 3rd, 7th and 14th day after burn elicitation. We also studied changes in angiogenesis in the capillary surrounding the basal layer and dermal papilla. The changes of HSP70 distribution and changes of p53 positive reaction were observed to investigate the changes of the stress in the skin as well. Result : The results indicated that GT has a significant impact on the antioxidant effect on skin cells of mice after bum elicitation by increasing SOD activity in vitro test. GT seems to decrease MIP-2 which induces neutrophil infiltration and promotes the angiogenesis dose-dependently. Furthermore, GT decreased HSP70, the expression of which was induced by elevated temperatures, and p53 which induce a apoptosis after stress. Conclusion : GT can be applied to burned skin through its antioxidant effect and skin regeneration property.

• 생명과학회지
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• 제29권4호
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• pp.455-460
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• 2019

이전 연구에서 우리는 Lactobacillus plantarum ilchiwhangchil 1785와 Lactobacillus plantarum ilchiwhangchil 2020 균주로 발효시킨 황칠나무 잎 가지 추출물의 효과적인 항균 활성을 확인하였다. 본 연구에서는 황칠나무 잎 가지 추출물을 L. plantarum ilchiwhangchil 1785와 L. plantarum ilchiwhangchil 2020 균주로 발효시킨 발효액이 인간모유두세포에 미치는 육모효과를 알아보았다. 황칠나무 잎 가지 발효 추출물의 농도가 1, $10{\mu}g/ml$ 범위에서 인간모유두세포는 세포사멸 증가 없이 유의적으로 세포 성장이 농도의존적으로 증가하였다. 또한 황칠나무 잎 가지 발효물로 처리한 세포가 대조군보다 세포성장이 활발함을 이중염색법으로 확인하였다. 더욱이 황칠나무 잎 가지 발효 추출물 처리한 세포는 처리하지 않은 세포에 비해 각각 18.84%, 23.31% 세포 이동성이 증가하였다. 고성능액체크로마토그래피(HPLC)를 이용하여 육모효과의 활성 물질을 분석하였다. 모발성장효과가 알려진 ${\beta}$-sitosterol 함량이 황칠나무 잎 가지 추출물에 비해 황칠나무 잎 가지 발효 과정에서 약 10배 증가되는 것을 확인하였다. 결과를 종합하면, 우리는 본 연구에서 황칠나무 잎 가지 추출물 발효액이 모발 성장을 촉진시킴을 확인하였다.

• 생약학회지
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• 제44권3호
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• pp.275-280
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• 2013

This study was carried out to investigate the effect of Origanum vulgare extracts on cell proliferation of human hair dermal papilla cell (HHDPC) using sulforhodamine B (SRB) assay, antioxidant activity by 1,1-diphenyl-2-picryl hydrazyl (DPPH) method, expression of insulin-like growth factor-1 (IGF-1) by analyzing reverse transcriptase polymerase chain reaction (RT-PCR) and hair growth in a shaving animal model of C57BL/6 mice topically applying with an amount of 0.1 mL once a day for 3 weeks. The mice were divided into 4 groups including normal group (saline, N), negative control group (dimethyl sulfoxide, NC), positive control group (5 mg/mL minoxidil, PC), and experimental group (Origanum vulgare extracts, OV). Treatment of OV didn't show cytotoxicity in HHDPC up to 10 ${\mu}g/mL$ and exhibited antioxidant activity with $IC_{50}$ of 31.0 ${\mu}g/mL$. IGF-1 expression in the skin was significantly (p<0.05) increased in the PC and OV compared to the N or NC. PC and OV also showed a prominently promoted hair regrowth compared to the N or NC in hair growth observation. The hair regrowth of OV was significantly higher than that of PC (p<0.05). Therefore, these results indicate that O. vulgare extracts effectively stimulated hair growth in an animal model.

• 대한화장품학회지
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• 제50권1호
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• pp.37-48
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• 2024

송라(Usnea diffracta Vain.)는 송라속(Usnea)에 속하는 지의류 중 하나이며, 이전 연구에서 항산화, 항균, 항염, 항종양 및 심혈관 보호 등의 약리학적 활성이 보고되어 있으나 피부 및 모발에서의 효능은 잘 알려져 있지 않다. 따라서 본 연구에서는 세포 수준에서 송라 추출물(UDE)의 항노화 및 모유두세포 증식에 대한 효과를 검증하였다. 실험 결과, 송라 추출물은 인간 섬유아세포에서 UVA에 의해 증가된 MMP-1의 발현 및 상위기전인 MAPKs (ERK, p38, JNK)와 AP-1 (c-Fos, c-Jun)의 활성을 유의적으로 감소하는 것을 확인하였다. 또한, 송라 추출물은 인간 모유두세포의 증식을 유의하게 증가시켰으며, 모발 성장인자인 VEGF 및 KGF의 mRNA 발현을 유의하게 증가시켰다. 이로 인하여, 모발 증식 및 성장인자의 발현에 관여하는 ERK/CREB의 인산화를 농도 의존적으로 증가시켰다. 송라 추출물의 주성분 확인을 위해 송라 추출물을 농축 후 Prep-LC를 이용하여 main peak로 나타난 분획을 분리 정제하였고, NMR 및 Mess 분석을 통해 diffractaic acid로 동정하였다. Diffractaic acid는 인간 섬유아세포에서 UVA에 의해 증가된 MMP-1의 발현을 유의적으로 감소시켰으며, 인간 모유두세포의 증식을 농도 의존적으로 증가시켰다. 이를 통해 송라 추출물은 항노화 및 모유두세포 활성 증가 효능을 갖는 화장품 천연소재로서의 활용 가능성을 입증하였다.

• 대한한의학회지
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• 제32권2호
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• pp.1-13
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• 2011

Background and Objective: Coptidis Rhizoma is a medicinal herb known for its antioxidant and anti-inflammatory effect. The purpose of this study was to examine the effects of CR on the experimental burn elicitation in vitro and in vivo. Material and Methods: In order to know the antioxidant effect on skin cell of mice after burn elicitation, superoxide dismutase (SOD) activity was measured. In vitro, the RAW 264.7 macrophage cells were treated with lipopolysaccharides for experimental inflammation. iNOS mRNA expression was observed after CR-treatment. In order to know effects on the skin regeneration in the burned mice, we counted the nitric oxide (NO) in blood. We also observed the histological structure in the epidermal basal layer and the dermal section, and we studied changes of angiogenesis in the capillaries surrounding the basal layer and dermal papilla. The changes of transcription of iNOS mRNA (inducible nitric oxide synthase mRNA) and changes of NF-${\kappa}$B (nuclear factor ${\kappa}$B) p65 positive reaction were also observed to investigate the changes of the stress in the skin. Results: The results indicated that CR has significant effects on the antioxidant effect on skin cells of mice after burn elicitation by increasing SOD activity in the in vitro test. It seemed that CR decreased the amount of NF-${\kappa}$B which induced the iNOS mRNA dose-dependently and suppress activating NO and angiogenesis. Furthermore, CR facilitated the process of skin recovery after experimental burn. Conclusion: CR can be applied for burned skin via antioxidant effect and skin regeneration.