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Experiment on the effect of Artemisia sieversiana extract on hair loss prevention and cell growth

  • Yang, Seungbo (Department of Korean Internal Medicine, College of Korean Medicine, Gachon University) ;
  • Jin, Chul (Department of Cardiology and Neurology, College of Korean Medicine, Kyung Hee University) ;
  • Kwon, Seungwon (Department of Cardiology and Neurology, College of Korean Medicine, Kyung Hee University) ;
  • Cho, Seung-Yeon (Department of Cardiology and Neurology, College of Korean Medicine, Kyung Hee University) ;
  • Park, Seong-Uk (Department of Cardiology and Neurology, College of Korean Medicine, Kyung Hee University) ;
  • Jung, Woo-Sang (Department of Cardiology and Neurology, College of Korean Medicine, Kyung Hee University) ;
  • Moon, Sang-Kwan (Department of Cardiology and Neurology, College of Korean Medicine, Kyung Hee University) ;
  • Park, Jung-Mi (Department of Cardiology and Neurology, College of Korean Medicine, Kyung Hee University) ;
  • Cho, Ki-Ho (Department of Cardiology and Neurology, College of Korean Medicine, Kyung Hee University) ;
  • Ko, Chang-Nam (Department of Cardiology and Neurology, College of Korean Medicine, Kyung Hee University)
  • Received : 2021.10.05
  • Accepted : 2022.02.22
  • Published : 2022.03.01

Abstract

Objectives: This study aimed to examine the safety, effects on proliferation of hair papilla cells, and anti-inflammatory and antioxidant mechanisms of Artemisia sieversiana Ehrh. ex Willd. (AS) extract. Methods: Safety tests through purity testing, acute toxicity tests, and repeated toxicity tests were performed using AS extract (ASE) which had been dried for over two years. Cell culture and proliferation tests were conducted; VEGF (vascular endothelial growth factor), bFGF (basic fibroblast growth factor), and EGF (epidermal growth factor) and protein expression analyses were performed for mechanistic evaluation; and inhibitory effects of ASE on the RNA expression of testosterone, 5𝛼-reductase, and aromatase was assessed. The anti-inflammatory and antioxidant efficacy of ASE was confirmed by measuring the levels of nitric oxide, inflammatory mediators (TNF-𝛼 and PGE2), inflammatory cytokines (IL-1𝛽, IL-6, and IL-8), and chemokine MCP-1. Results: The safety of ASE was confirmed. The mechanism of cell proliferation in human hair follicle dermal papilla cells involved the promotion of VEGF, bFGF, and EGF expression. ASE decreased mRNA expression of testosterone, 5𝛼-reductase, and aromatase-1 in a concentration-dependent manner. PGE2 and TNF-𝛼 production by inflammatory mediators was also significantly decreased in a concentration-dependent manner, and inflammatory cytokine and chemokine expression was inhibited. Conclusions: ASE is suggested to promote papillary cell growth at the cellular level, to suppress expression of various enzymes involved in hair cycle and cell death, and to inhibit hair loss through anti-androgen, anti-inflammatory, and antioxidant effects.

Keywords

Acknowledgement

We would like to thank Editage (www.editage.co.kr) for English language editing

References

  1. Yong SH, Park DJ, Yang WH, Seol YW, Choi EJ, Jeong MJ, et al. (2019). Cryopreservation of sievers wormwood (Artemisia sieversiana Ehrh. Ex Willd.) seeds by vitrification and encapsulation. Forest Science and Technology, 15(4), 180-186. https://doi.org/10.1080/21580103.2019.1658647
  2. Madani, S., & Shapiro, J. (2000). Alopecia areata update. J Am Acad Dermatol, 42(4), 549-566; quiz 567-570. https://doi.org/10.1067/mjd.2000.103909
  3. Randall, V. A., Hibberts, N. A., Thornton, M. J., Hamada, K., Merrick, A. E., Kato, S., et al. (2000). The hair follicle: a paradoxical androgen target organ. Horm Res, 54(5-6), 243-250. https://doi.org/10.1159/000053266
  4. Jeong IK, Jo HY, Kim TH, Kim NS, Jeong HS, & Lee CH. (2009). Experimental Studies on the Hair Growth Activity of Extracts of Pinelliae Rhizoma in Spontaneous Alopecia Model and Normal C57BL/ 6N Mice. Korean J Orient Physiol Pathol, 23(1), 84-92.
  5. Kwon KS, Lee MW, Jeong IK, Jeong HS, Song BY, Song JM, et al. (2009). Experimental studies on the hair growth activity of fractions and extract of arisaematis rhizoma in C57B/6N mice. Korean J Orient Physiol Pathol, 23(3), 619-630.
  6. Roh HC, & Roh SS. (2002). Studies on the effect of Sophora flavescens extract on the hair growth stimulation and acne inhibition. J Korean Med Ophthalmol Otolaryngol Dermatol, 15(1), 96-126.
  7. Kim PS, Kim HT, Roh SS, & Hwang CY. (2004). Effect of DanGuiBoHyulTangGami -Bang on the Alopecia and Hair Growth Stimulation. J Korean Med Ophthalmol Otolaryngol Dermatol, 17(3), 38-60.
  8. Yun JH, Kim NK, Lim KS, Roh SS, & Hwang CY. (2004). Study on the effect of Gamissanghwa-tang and each medicinal plant extract for the hair growth of the mice using in vivo and in vitro test. Korean J. Orient. Physiol. Pathol, 18(2), 561-570.
  9. Liu, S.-J., Liao, Z.-X., Tang, Z.-S., Cui, C.-L., Liu, H.-B., Liang, Y.-N., et al. (2017). Phytochemicals and biological activities of Artemisia sieversiana. Phytochemistry Reviews, 16(3), 441-460. https://doi.org/10.1007/s11101-016-9475-z
  10. Chen, H., Miao, Q., Geng, M., Liu, J., Hu, Y., Tian, L., et al. (2013). Anti-Tumor Effect of Rutin on Human Neuroblastoma Cell Lines through Inducing G2/M Cell Cycle Arrest and Promoting Apoptosis. ScientificWorldJournal, 269165. https://doi.org/10.1155/2013/269165
  11. Guo, N., Xu, Y., & Cao, Z. (2015). Absinthin attenuates LPS-induced ALI through MIP-1α-mediated inflammatory cell infiltration. Exp Lung Res, 41(9), 514-524. https://doi.org/10.3109/01902148.2015.1093566
  12. Kiprono, P.C., Kaberia, F., Keriko, J.M., & Karanja, J.N. (2000). The in vitro anti-fungal and anti-bacterial activities of beta-sitosterol from Senecio lyratus (Asteraceae). Z Naturforsch C J Biosci, 55(5-6), 485-488. https://doi.org/10.1515/znc-2000-5-629
  13. Kumar J, Mishra GP, Naik PK, Murkute AA, & Srivastava RB. (2011). Genomic DNA isolation from Artemisia species grown in cold desert high altitude of India. Afr J Biotechnol, 10(37), 7303-7307. https://doi.org/10.5897/AJB10.2031
  14. Serra MF, Diaz BL, Barreto EO, Pereira AP, Lima MC, Barbosa-Filho JM, et al. (1997). Anti-allergic properties of the natural PAF antagonist yangambin. Planta Med, 63(3), 207-212. https://doi.org/10.1055/s-2006-957654.
  15. Nakai, M., Harada, M., Nakahara, K., Akimoto, K., Shibata, H., Miki, W., et al. (2003). Novel antioxidative metabolites in rat liver with ingested sesamin. J Agric Food Chem, 51(6), 1666-1670. https://doi.org/10.1021/jf0258961.
  16. Kang, J., Xie, C., Li, Z., Nagarajan, S., Schauss, A.G., Wu, T., et al. (2011). Flavonoids from acai (Euterpe oleracea Mart.) pulp and their antioxidant and anti-inflammatory activities. Food Chem, 128(1), 152-157. https://doi.org/10.1016/j.foodchem.2011.03.011.
  17. Mounsey, A.L., & Reed, S.W. (2009). Diagnosing and treating hair loss. Am Fam Physician, 80(4), 356-362.
  18. Hagemann, T., Schlutter-Bohmer, B., Allam, J.P., Bieber, T., & Novak, N. (2005). Positive lymphocyte transformation test in a patient with allergic contact dermatitis of the scalp after short-term use of topical minoxidil solution. Contact Dermatitis, 53(1), 53-55. https://doi.org/10.1111/j.0105-1873.2005.00456b.x.
  19. Diani, A.R., Mulholland, M.J., Shull, K.L., Kubicek, M.F., Johnson, G.A., Schostarez, H.J., et al. (1992). Hair growth effects of oral administration of finasteride, a steroid 5 alpha-reductase inhibitor, alone and in combination with topical minoxidil in the balding stumptail macaque. J Clin Endocrinol Metab, 74(2), 345-350. https://doi.org/10.1210/jcem.74.2.1309834.
  20. Rathnayake, D., & Sinclair, R. (2010). Male androgenetic alopecia. Expert Opin Pharmacother, 11(8), 1295-1304. https://doi.org/10.1517/14656561003752730.
  21. Korean Society of Pathologists. (1998). Textbook of Pathology (3rd ed.). Seoul: Komoonsa.
  22. Vinay Kumar, Abul K. Abbas, Nelson Fausto, & Jon Aster. (2011). Robbins & Cotran Pathologic Basis of Disease (8th ed.). Philadelphia: Saunders.
  23. Southan, G.J., & Szabo, C. (1996). Selective pharmacological inhibition of distinct nitric oxide synthase isoforms. Biochem Pharmacol, 51(4), 383-394. https://doi.org/10.1016/0006-2952(95)02099-3.
  24. Chawla, A., Nguyen, K.D., & Goh, Y.P. (2011). Macrophage-mediated inflammation in metabolic disease. Nat Rev Immunol, 11(11), 738-749. https://doi.org/10.1038/nri3071.
  25. Aktan, F. (2004). iNOS-mediated nitric oxide production and its regulation. Life Sci, 75(6), 639-653. https://doi.org/10.1016/j.lfs.2003.10.042.
  26. Laskin, D.L., & Pendino, K.J. (1995). Macrophages and inflammatory mediators in tissue injury. Annu Rev Pharmacol Toxicol, 35, 655-677. https://doi.org/10.1146/annurev.pa.35.040195.003255.
  27. Takeda, K., & Akira, S. (2015). Toll-like receptors. Curr Protoc Immunol, 109, 14.12.11-14.12.10. https://doi.org/10.1002/0471142735.im1412s109.
  28. Barton, G.M., & Medzhitov, R. (2003). Toll-like receptor signaling pathways. Science, 300(5625), 1524-1525. https://doi.org/10.1126/science.1085536.
  29. Kim, Y.W., Zhao, R.J., Park, S.J., Lee, J.R., Cho, I.J., Yang, C.H., et al. (2008). Anti-inflammatory effects of liquiritigenin as a consequence of the inhibition of NF-kappaB -dependent iNOS and proinflammatory cytokines production. Br J Pharmacol, 154(1), 165-173. https://doi.org/10.1038/bjp.2008.79.
  30. Wink, D.A., and Mitchell, J.B. (1998). Chemical biology of nitric oxide: Insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide. Free Radic Biol Med, 25(4-5), 434-456. https://doi.org/10.1016/s0891-5849(98)00092-6.
  31. Janeway CA Jr, Travers P, Walport M, & Shlomchik MJ. (2001). Immunobiology: The Immune System in Health and Disease. (5th ed). New York: Garland Science.