• Journal of Microbiology and Biotechnology
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• v.27 no.11
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• pp.2019-2027
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• 2017

Recently, cabbage kimchi has occasionally been associated with the foodborne diseases of enteric viruses such as human norovirus (HuNoV). This study aimed to evaluate the correlation between microbial/physicochemical properties and persistence of HuNoV in experimentally contaminated cabbage kimchi fermented and stored at $4^{\circ}C$ or $10^{\circ}C$ for 28 days. Changes in organic acid content, lactic acid bacteria (LAB), acidity, pH, and salinity were analyzed. The recovery of structurally intact HuNoV was examined for up to 28 days post-inoculation, using a NoV GII.4 monoclonal antibody-conjugated immuno-magnetic separation method combined with quantitative real-time reverse transcription polymerase chain reaction. On day 0, LAB loads were $4.70log_{10}$ colony forming units/g and HuNoV GII.4 titers were $2.57log_{10}\;genomic\;copies/{\mu}l$, at both temperatures. After 28 days, intact HuNoV titers decreased to 1.58 ($4^{\circ}C$) and $1.04(10^{\circ}C)log_{10}\;genomic\;copies/{\mu}l$, whereas the LAB density increased. This correlated with a gradual increase in lactic acid and acetic acid at both temperatures. Our findings support a statistical correlation between changes in physicochemical properties and the recovery of structurally intact HuNoV GII.4. Moreover, we determined that the production of organic acid and low pH could affect HuNoV GII.4 titers in cabbage kimchi during fermentation. However, HuNoV GII.4 was not completely eliminated by microbial/physicochemical factors during fermentation, although HuNoV GII.4 was reduced. Based on this, we speculate that the persistence of HuNoV GII.4 may be affected by the continually changing conditions during kimchi fermentation.

• Journal of Life Science
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• v.20 no.12
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• pp.1896-1901
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• 2010

This study was conducted using quantitative real-time PCR using Lactobacilli as probiotics. Quantitative real-time PCR (RT PCR) was conducted via a method involving SYBR Green 1 and a probe. Plasmid DNA was cloned using the 16S-23S rRNA intergenic species region. Gene clones were diluted from $10^2$ to $10^{10}$. Standard curves were constructed via Ct values obtained from the results of Real-time PCR via the aforementioned SYBR Green 1 and probe method. Plasmid DNA was also cloned using the 16S-23S rRNA intergenic species region and the gene clones were diluted from $10^2$ to $10^{10}$ copy numbers via the probe method. Using RT PCR, a standard curve of plasmid DNA copy numbers was also determined. The slope value for the Y-axis intercept and $R^2$ value were measured as -3.346, 33.18, and 0.993, respectively, via the first method. For the second method, the slope value for the Y-axis intercept and $R^2$ were -3.321, 31.10 and 0.995, respectively. The PCR inhibitor could not express the detection curve at a copy number over $10^{10}$ via either method, owing to high DNA density. The DNA extract from probiotics was diluted without pre-culturing, and 16 products were amplified via both methods. The Ct value was 11.06~18.12 in the first method and 16.74~22.11 in the second method. Measured probiotics and log copy values were largely similar among the methods used. It was concluded that both methods are effective for analysis, but further research will be required to verify the optimal method.

• The Journal of the Korean dental association
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• v.54 no.12
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• pp.971-984
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• 2016

The increase rate of dentists' competition is very fast at metropolitan areas in South Korea. We compare metropolitan and rural parameters to investigate the relation between competition and revenue variation. The competition and revenue variables of 73 metropolitan and 75 rural areas were calculated from 2010 Census of Service Industry microdata which include non-insurance revenues of dental clinics. Independent sample t-test results showed that the level of competition among dental clinics in metropolitan areas is higher. The lowest and the low ranked revenues are higher in rural areas. The highest and the average revenues are higher in metropolitan areas. But, 25 percentile and median revenues has no significant difference between two areas. Simple log linear regression results showed that the number of clinics could explain the distribution of revenues in both areas better than the density of active dentists and Herfindahl-Hirschman index. In the areas with many clinics have high maximum and average revenues and low minimum revenues. The increasing rate of maximum revenues is higher in metropolitan areas though the decreasing rate of minimum revenues is higher in rural areas. Metropolitan areas have higher Gini coefficients than rural areas, but the increasing rate of Gini coefficients is lower than rural areas. Findings from this study are useful reference when the dentists select the opening areas. One is that the median revenues between metropolitan and rural areas have no significant difference. The other is that the rural areas ensure the more stable and uniform revenues. The results would help to relieve the consumptive competition among dentists and to achieve the distributional efficiency of dental human resources.

• Parasites, Hosts and Diseases
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• v.49 no.1
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• pp.9-15
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• 2011

To understand the distribution of Giardia cysts in drinking water supplies in Seoul, Korea, we collected water samples quarterly at 6 intakes in the Han River, its largest stream and 6 conventional water treatment plants (WTPs) serving drinking water, from 2000 to 2009. Giardia cysts in each of 10 L water were confirmed in 35.0% of intake water samples and the arithmetic mean was 1.65 cysts/10 L (range 0-35 cysts/10 L). The lowest cyst density was observed at Paldang and Kangbuk intakes, and the pollution level was higher at 4 intakes downstream. It seemed that these 4 intakes were under influence of Wangsuk stream at the end of which cysts were found in all samples with the mean of 140 cysts/10 L. The annual mean number of cysts was 0.21-4.21 cysts/10 L, and the cyst level at the second half of the 10 years was about 1/5 of that at first half on average. The cysts were more frequently found in winter, and their mean density was 3.74 cysts/10 L in winter and 0.80-1.08 cysts/10 L in other seasons. All finished water samples collected at 6 WTPs were negative for Giardia in each of 100 L sample for 10 years and cyst removal by physical process was average 2.9-log. It was concluded that conventional water treatment at 6 WTPs of Seoul appears to remove the cysts effectively under the present level of their source water. Domestic wastewater from the urban region could be an important source of Giardia pollution in the river.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.22 no.3
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• pp.171-180
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• 2010

Analysis on the temporal variation of the stratification is carried out by using the continuous water temperature and salinity data observed in the coastal monitoring buoy in Mikawa Bay, Japan. The main analysis results are as follows. The stratification pattern have an obvious and dominant 1-year period variation and the occurrence frequency (days) are exponentially decreased as the stratification intensity (SI) defined as the density difference between surface and bottom layers linearly increases. The frequency distribution function of the SI is presumably close to the log-normal function type or exponential function type. From the water temperature and salinity scatter diagram analysis, the line and loop type patterns are shown in the bottom and surface layers, respectively. In addition, the analysis of the SI estimation show that the error bound in case of using the weekly-monitoring data is about 4.45 times greater than that in case of using the continuous (daily) monitoring data.

• Journal of Korea Water Resources Association
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• v.35 no.1
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• pp.25-36
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• 2002

Window Probability Matching Method(WPMM) is achieved by matching identical probability density of rain intensities and radar reflectivities taken only from small window centered about the gage. The equation of $Z_{e}-R$ relationship is obtained and compared with data between a DWSR-88C radar and high density rain gage networks within 150km from radar site in summer season, 1998. The probability density of radar effective reflectivity is distributed with high frequency near 15dBZ. The frequency distribution of rain intensities shows that rain intensity is lower than 10mm/hr in most part of radar coverage area. As the result of $Z_{e}-R$ relationship using WPMM, curved line has shown to the log scale spatially and it can be explained more flexible than any straight-line power laws at the transformation to the rainfall amount from $Z_e$ value. During 3 months, total radar cumulative rainfall amount estimated by $Z=200R^{1.6}$ and WPMM relationships are 44 and 80 percentages of total raingage amount, respectively. Therefore, $Z_{e}-R$ relationships by WPMM may be widely needed a statistical method for the computation of accumulated precipitation.

• Journal of Korean Society of Forest Science
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• v.109 no.4
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• pp.468-476
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• 2020

we developed a stand density management diagram for Quercus variabilis in order to predict the final cutting ages of coppice forests based on management objectives. The sample data were classified into two groups: 603 data points for analysis and 113 data points for verification. Using these data, a stand density management diagram was constructed and a goodness-of-fit test was performed. The explanatory power (R2) of the constituent models for the stand density management diagram was 0.732 for the equivalent height curve and 0.990 for the equivalent diameter curve. According to the analysis of the final cutting age rangeof the highest production, the final cutting ages for 900 buds remaining per hectare was 42-44 years, and that for the 1,800 buds remaining per hectare was 38-42 years. With the third-grade log set as the production target, the final cutting age range with site index 16 and 14 was 25-28 years and 29-33 years, respectively. The results of this study provide baseline data for establishing a management plan for Q. variabilis coppice stands.

• Journal of the Korean Data and Information Science Society
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• v.25 no.1
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• pp.177-185
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• 2014

Under bivariate normal distribution assumptions, the interaction and quadratic terms are needed in the logistic regression model with two predictors. However, depending on the correlation coefficient and the variances of two conditional distributions, the interaction and quadratic terms may not be necessary. Although the need for these terms can be determined by comparing the two scatter plots, it is not as useful for interaction terms. We explore the structure and usefulness of the 3-D residual plot as a tool for dealing with interaction in logistic regression models. If predictors have an interaction effect, a 3-D residual plot can show the effect. This is illustrated by simulated and real data.

• Steel and Composite Structures
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• v.19 no.1
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• pp.173-190
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• 2015

This study presents an analytical investigation on the seismic response of a medium-rise buckling-restrained braced frame (BRBF) under the near-fault ground motions. A seven-story BRBF is designed as per the current code provisions for five different combinations of brace configurations and beam-column connections. Two types of brace configurations (i.e., Chevron and Double-X) are considered along with a combination of the moment-resisting and the non-moment-resisting beam-to-column connections for the study frame. Nonlinear dynamic analyses are carried out for all study frames for an ensemble of forty SAC near-fault ground motions. The main parameters evaluated are the interstory and residual drift response, brace displacement ductility, and plastic hinge mechanisms. Fragility curves are developed using log-normal probability density functions for all study frames considering the interstory drift ratio and residual drift ratio as the damage parameters. The average interstory drift response of BRBFs with Double-X brace configurations significantly exceeded the allowable drift limit of 2%. The maximum displacement ductility characteristics of BRBs is efficiently utilized under the seismic loading if these braces are arranged in the Double-X configurations instead of Chevron configurations in BRBFs located in the near-fault regions. However, BRBFs with the Double-X brace configurations exhibit the higher interstory drift and residual drift response under near-fault ground motions due to the formation of plastic hinges in the columns and beams at the intermediate story levels.

• Korea-Australia Rheology Journal
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• v.15 no.1
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• pp.35-41
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• 2003

It is expected that particle size distribution of any portion obtained through screening, is of more uniform than that of the original mixture, typically following such as log-normal, Rosin-Rammler distributions and so on. In this study, therefore, a new relation between parameters of the uniform distribution and flow characteristics of the coarse particle suspensions is derived based on the continuous polydisperse model (Ookawara and Ogawa, 2002b), which is derived from the discrete polydisperse model (Ookawara and Ogawa,2002a). The derived model equation predicts a linear increase of viscosity with shear rate, viz., dilutant flow characteristics. Further, the increase of viscosity is expected to be proportional to the square of volume fraction of particles, and to show the linear dependency on density and average diameter of particles. It is also shown that the uniform distribution model includes additional term that expresses the effect of distribution width. For verification of the model, the experimental results of Clarke (1967) are cited as well as in our previous work for the monodisperse model (Ookawara and Ogawa,2000) since most parameters were varied independently in his work. It is suggested that the newly introduced term expands the applicable range compared with the monodisperse model.