• 제목/요약/키워드: defined medium

검색결과 487건 처리시간 0.034초

Enantioselective N-Acetylation of 3-Amino-3-phenylpropionic Acid by Cell-free Extracts of Streptomyces neyagawaensis

  • Chung, Myung-Chul;Lee, Ho-Jae;Lee, Choong-Hwan;Chun, Hyo-Kon;Kho, Yung-Hee
    • Journal of Microbiology and Biotechnology
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    • 제7권5호
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    • pp.329-332
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    • 1997
  • Cell-free extracts of Streptomyces neyagawaensis SL-387 grown on a chemically defined medium supplemented with DL-3-amino-3-phenylpropionic acid (APP) produced N-acetyl-APP (Ac-APP) in the presence of APP and acetyl coenzyme A. The APP obtained by acid hydrolysis of the Ac-APP was D-configuration: $[\alpha]_D+6.5^{\circ}(H_2O)\;at\;20^{\circ}C$, optical purity 92% enantiomeric excesses (ee). These results suggest that an N-acetyltransferase exists in the cell-free extract as a novel enzyme with specificity for D-APP.

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조도효과를 고려한 다공질 공기베어링의 정강성 특성에 관한 수치해석 연구 (NUMERICAL INVESTIGATION ON STATIC STIFFNESS CHARACTERISTICS OF POROUS AIR BEARING CONSIDERING ROUGHNESS EFFECTS)

  • 권혁록;이성혁;이재응
    • 한국전산유체공학회지
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    • 제13권2호
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    • pp.62-67
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    • 2008
  • This study aims to investigate numerically the static stiffness characteristics of porous air bearing and to estimate appropriate permeability values of porous medium. In particular, a new roughness model is proposed and implemented into the commercial CFD code (FLUENT Ver. 6.2) by using C language based user subroutine. The predicted results are extensively compared with experimental data. The roughness model is also validated through comparison with the results from open literature. It is found that the predictions for static stiffness are in good agreement with experimental data. Therefore, the suggested model based on the roughness Reynolds number can be used in studying the stiffness characteristics of porous air bearing effectively. In addition, numerical simulations of various diameter size and conditions are conducted. According the results, it is expected that the static stiffness of porous air bearing has the non-linear characteristics.

열린 수학 학습을 위한 게임의 교육적 활용 가능성 탐색 (An exploratory study of the Educational Simulation Games for open mathematic learning)

  • 김나영
    • 대한수학교육학회지:수학교육학연구
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    • 제8권1호
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    • pp.327-350
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    • 1998
  • The purpose of this study is to examine the applicability of educational games to open mathematic learning and consideration to the position of educational games in mathmatical education as powerful technology. For these purposes, previous literatures about games are reviewed. The concept of simulation games are defined and explore the characteristics of games includings game structure and process. And some typical educational games - Israel games, ORDA - are introduced. The main focus of the deliberation and survey of previous literatures is educational games as meaningful learning medium of mathematics and other subjuct matters. Especially educational games take a meaningful role for an implication of applicability of games especially for higher order thinking skills like problem solving, decision making, and creativity. To realize this alternative learning method of mathematics, first of all the attituce of teachers are have to change and accumulate field studies.

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Time harmonic analysis of dam-foundation systems by perfectly matched layers

  • Khazaee, Adib;Lotfi, Vahid
    • Structural Engineering and Mechanics
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    • 제50권3호
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    • pp.349-364
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    • 2014
  • Perfectly matched layers are employed in time harmonic analysis of dam-foundation systems. The Lysmer boundary condition at the truncation boundary of the PML region has been incorporated in the formulation of the dam-foundation FE model (including PML). The PML medium is defined in a way that the formulation of the system can be transformed into time domain. Numerical experiments show that applying Lysmer boundary conditions at the truncation boundary of the PML area reduces the computational cost and make the PML approach a more efficient technique for the analysis of dam-foundation systems.

A Selected Multicast Polling Scheme for IEEE 802.11 Point Coordination Function

  • Lee Sang Don;Cho Chi Woo;Kim Kyung Jun;Kim Hyun Sook;Han Ki Jun
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2004년도 학술대회지
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    • pp.536-539
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    • 2004
  • IEEE 802.11 Point Coordination Function (PCF) mode is defined to support time bounded traffic, such as voice in wireless LANs. The poll scheduling plays an important role in IEEE 802.11 PCF mode operation. This paper proposed a Multicast Polling Scheme (MPC) to increase the performance of wireless LANs. Moreover, we proposed a polling schedule scheme for our proposed multi-poll to serve real-time traffic. The results show that the proposed mechanism is more efficient than the original IEEE 802.11 PCF.

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글라스하우스의 파빌리온, 폴리, 인스톨레이션의 특성 (The Features of the Pavilions, Follies, and Installations of the Glass House)

  • 김란수
    • 건축역사연구
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    • 제26권1호
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    • pp.71-82
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    • 2017
  • Pavilions, follies, and installations provide a place with a new experience beyond that of simple garden architecture. From this point of view, this study tried to analyze the constructs in the Glass House site, which Philip Johnson has built for 50 years. After Chapter 1 Introduction, which summarized the background of the study, Chapter 2 investigated the design background of the landscape and the types of the constructs there. It also, studying literature on pavilions, follies, and installations, defined the basic meanings of them. Chapter 3 identified the features of each construct through the case studies of it, analyzing Johnson's intentions on it. These features are such as the applications of classical follies, the quotations of architectural history, fusion with art, architectural experiments, and the monuments of personal history. In conclusion, this study, finding the site specificity as a common feature of pavilions, follies, and installations, referred to two aspects of this, which are not only physical placeness but also cultural media.

Possible Association of Indole-3-Acetic Acid Production by Xanthomonas axonopodis pv. glycines with Development of Pustule Disease in Soybean

  • Kim, Hong-Suk;Park, Hyoung-Joon;Heu, Sunggi;Jung, Jin
    • Journal of Applied Biological Chemistry
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    • 제44권4호
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    • pp.173-176
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    • 2001
  • This report concerns the role of indole-3-acetic acid (IAA) in bacterial pustule disease of soybean. Pustule production in soybean leaves caused by Xanthomonas axonopodis pv. glycines was accompanied by a drastic increase in IAA content of host tissues. The phytopathogenic bacterium synthesized IAA in a tryptophan concentration-dependent manner when grown in a defined minimal medium. In complex media, however, the pathogen showed no response to tryptophan feeding, implying that the bacterial biosynthetic machinery of IAA is strictly regulated by nutrient availability of its growth environments. The results may suggest that IAA of bacterial origin and tryptophan of plant origin be involved in the process of pustule symptom development in soybean.

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Ultrasonic Measurement of Interfacial Layer Thickness of Sub-Quarter-Wavelength

  • Kim, No-Hyu;Lee, Sang-Soon
    • 비파괴검사학회지
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    • 제23권6호
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    • pp.577-582
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    • 2003
  • This paper describes a new technique for thickness measurement of a very thin layer less than one-quarter of the wavelength of ultrasonic wave used in the ultrasonic pulse-echo measurements. The technique determines the thickness of a thin layer in a tapered medium from constructive interference of multiple reflection waves. The interference characteristics are derived and investigated in theoretical and experimental approaches. Modified total reflection wave g(t) defined as difference between total and first reflection waves increases in amplitude as the interfacial layer thickness decreases down to zero. A layer thickness less than one-tenth of the ultrasonic wavelength is measured using the maximum amplitude of g(t) with a good accuracy and sensitivity. The method also requires no inversion process to extract the thickness information from the waveforms of reflected waves, so that it makes possible to have the on-line thickness measurement of a thin layer such as a lubricating oil film in thrust bearings and journal bearings during manufacturing process.

An Outlook on Rotordynamic Pump Theory Development

  • Ni, Yongyan;Pan, Zhongyong
    • International Journal of Fluid Machinery and Systems
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    • 제10권2호
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    • pp.99-118
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    • 2017
  • ECHO progress was defined to depict the rotordynamic pump theory development. Experience (E) era for pumps lasted nearly one and a half hundred years before the Industrial Revolution due to the low rotation speed of motor and undeveloped manufacture ability. Classic (C) theory referring to quasi-static performance as well as the items those were not able to be steadily resolved under the level were briefly and sophisticated outlined. Since 1962, flow instabilities and the dynamic responses had come into main attention with the development of the modern technologies such as ballistic missile, rocket and space shuttle main engine, and were finally heuristically (H) elucidated by talented scholars and researchers. Recently, new applications for the pumps open (O) to the surrounding fluid and diversity of the medium such as multiphase flow need more studies and some examples were briefly introduced to display the potential problems lastly.

Enterococcus faecalis RKY1 이 생산하는 Fumarate Reductase의 특성 (Characteristics of Fumarate Reductase from Enterococcus faecalis RKY1)

  • 박미란;김도만;류화원;이진하
    • KSBB Journal
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    • 제15권3호
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    • pp.318-322
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    • 2000
  • An oxygen-sensitive fumarate reductase has been purified from the cytosol fraction of the Enterococcus faecalis RKY1 grown anaerobically on a defined medium containing glycerol and fumarate. A major portion of the purification was performed with employing Triton X-100 and reducing agents by Phenyl-sepharose CL-4B DEAE-sepharose and Dephadex G-150 The final activity was 0.42 unit/mg. The deduced molecular mass of active band was 66 kDa. The optimal pH and temperature for the activity were 7.0 and 38$^{\circ}C$ respectively. The enzyme activity was not affected by 1mM metal ions such as bacl2 $.$2H2O HgCl2 MnCl2$.$4H2O ZnCl2 CuCl2$.$2H2O Mgcl2$.$6H2O FeSo4$.$7H2O and by EDTA. Partially purified enzyme ws yellow in color ; spectroscopic study indicated the presence of flavins as a cofactor.

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