• The Korean Journal of Physiology and Pharmacology
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• 제11권5호
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• pp.175-182
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• 2007

Members of prostaglandin(PG) E-series elicit cellular effects mainly through adenylyl cyclase-cAMP signaling. The role of $PGE_2$-induced increase in cAMP has been shown to be compartmentalized in the cardiac myocytes: $PGE_2$-induced increase of cAMP is not involved in the control of cardiomyocytic contraction. The purpose of the present study was to define the effect of $PGE_1$ on the cGMP levels and the role of $PGE_1$ in the atrial secretory function. Experiments were performed in perfused beating rabbit atria and atrial contractile responses, cGMP and cAMP efflux, and atrial natriuretic peptide(ANP) secretion were measured. $PGE_1$ increased cGMP as well as cAMP efflux concentration in a concentration-dependent manner, however, no significant changes in atrial secretory responses were observed(with $1.0{\mu}M\;PGE_1$; for cGMP, $144.76{\pm}37.5%$, n=11 versus $-16.81{\pm}4.76%$, n=6, control, p<0.01; for cAMP, $187.60{\pm}41.52%$, n=11 versus $7.38{\pm}19.44%$, n=6, control, p<0.01). $PGE_1$ decreased atrial dynamics slightly but transiently, whereas $PGE_2$ showed similar effects but with lower potency. Isoproterenol increased atrial cAMP efflux(with 2.0 nM; $145.71{\pm}41.89$, n=5 versus $7.38{\pm}19.44%$, n=6, control, p<0.05) and mechanical dynamics and decreased ANP secretion. The $PGE_1$-induced increase in cGMP efflux showed a bell-shaped concentration-response curve. $PGE_1$-induced increase of cGMP efflux was not observed in the presence of L-NAME, an inhibitor of nitric oxide(NO) synthase, or ODQ, an inhibitor of NO-sensitive guanylyl cyclase. L-NAME and ODQ showed no significant effect on the $PGE_1$-induced transient decrease of atrial dynamics. These data indicate that $PGE_1$ increases cGMP levels via NO-soluble GC signaling in the cardiac atrium and also show that $PGE_1$-induced increases in cGMP and cAMP levels are not involved in the regulation of atrial secretory and contractile functions.

• 기술혁신학회지
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• 제15권2호
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• pp.242-261
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• 2012

본 고에서는 연구기관에서 개발된 기술의 기술이전 가격결정을 위해 초기단계 기술의 가치평가를 포함하여 연구개발이 진행됨에 따른 단계별 가치평가 방법을 적용하기 위한 개념적 틀을 구상해보고자 한다. 전통적인 기술가치평가는 암묵적으로 기술이 제품에 적용되는 경쟁기술인 상태를 가정하는 경우가 많다. 그러나 초기단계 기술 또는 경쟁환경에 노출되기 이전 단계의 기술에 대한 가치는 단일의 평가방법만으로 평가하는 데 한계가 존재한다. 따라서 이와 같은 초기단계 기술에 대한 가치평가를 수행하기 위한 방법론 적용의 프레임워크를 제시하고자 하였다.

• 유통과학연구
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• 제10권4호
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• pp.7-11
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• 2012

"Great things in business are never done by one person; they're done by a team of people." By Steve Jobs, 2003. As define by Linda Naiam - Creativity is the act of turning new and imaginative ideas into reality. Creativity involves two processes: thinking, then producing. Innovation is the production or implementation of an idea. If you have ideas, but don't act on them, you are imaginative but not creative. So in order to maintain the pace with the changing business scenario and coping with the competition Innovation and Creativity is considered a mandatory tool for a business to exist and grow in market. Whether a company is Employee centric or Business centric Innovation and creativity has to have its space in order to keep a business ahead of others in the Market. Also it's not just the competition which has led to the Innovation and Creativity in Business practices it's also the demanding chunk of consumers and customers who are aware and prefer maximum choices before making a final deal. Another reason as to why there is a change in business practices is the globalization of businesses where you need to rope in the Innovative ideas to launch and sustain in new market. There had been tremendous shift in business practices but to give a room to innovative ideas and implement that creativity need ample to space and vision along with an attitude where in you can resist for getting an immediate results from innovative business practices. Corporate Creativity is characterized by the ability to perceive the world in new ways, to find hidden patterns, to make connections between seemingly unrelated phenomena, and to generate solutions. Generating fresh solutions to problems, and the ability to create new products, processes or services for a changing market, are part of the intellectual capital that give a company its competitive edge. Creativity is a crucial part of the innovation equation. The innovation and creativity is not limited to any area of business, it can start from your waiting lounge to your board room meeting depending upon how the things are perceived and implemented for the betterment of people and business. The purpose of this research is to understand the latest creative business approaches and practices that organizations are following to be different from their competitors. Also this shift from generic business practices to the Innovative and Creative approach seems to take the business into new world. This approaches means starting from the bottom of the Pyramid and finally touching the pinnacles in Innovation and creativity. The paper will discuss on the various areas of business where in innovative approaches can be roped in and sets new bench mark altogether in the business arena.

• Fisheries and Aquatic Sciences
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• 제8권3호
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• pp.151-160
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• 2005

Genomic DNA samples isolated from Fenneropenaeus chinensis (fleshy prawn; FP) and Palaemon gravieri (Chinese ditch prawn; CDP) collected in the West Sea, off the Korean Peninsula, at Buan, were PCR-amplified repeatedly. The sizes of the DNA fragments generated by seven different primers varied from 50 bp to 1,600 bp. We identified 358 fragments for the FP species and 301 fragments for the CDP species. There were 18 polymorphic fragments (5.03$\%$) for the FP species and 12 (3.99$\%$) for the CDP species. In total, 66 common fragments (average of 9.4 fragments per primer) were observed for the FP species and 44 fragments (average of 6.3 fragments per primer) were observed for the CDP species. The numbers of specific fragments seen for the FP species and CDP species were 38 and 47, respectively. The complexity of the banding patterns varied dramatically between the primers and the two species. In the FP species, a specific fragment of approximately 1,200 bp generated by primer OPB-04 exhibited inter-individual-specific characteristics that were indicative of DNA polymorphisms. Moreover, in the CDP species, a major fragment of approximately 550 bp generated by primer OPB-20 was found to be specific for the CDP. The average bandsharing value between the two prawn species was 0.421$\pm$0.006, and ranged from 0.230 to 0.611. The dendrogram obtained using the data from the seven primers indicated seven genetic clusters: cluster 1, FLESHY 01, 02, 03, and 04; cluster 2, FLESHY 05, 06, and 07; cluster 3, FLESHY 08, 09, 10, and 11; cluster 4, DITCH 13, 14, 16, and 18; cluster 5, DITCH 12, 15, and 17; cluster 6, DITCH 19, 20, and 21; and cluster 7, DITCH 22. The genetic distance between the two prawn species ranged from 0.071 to 0.642. Thus, RAPD-PCR analysis revealed a significant genetic distance between the two prawn species. Using various arbitrary primers, RAPD-PCR may be applied to identify specific/polymorphic markers that are particular to a species and geographic population, and to define genetic diversity, polymorphisms, and similarities among shrimp species.

• 한국정보과학회논문지:소프트웨어및응용
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• 제33권8호
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• pp.718-729
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• 2006

일정 분야의 문서들에서 그 분야 특정을 반영하는 전문용어를 자동으로 인식하는 연구에 대한 관심이 증가하고 있다. '전문용어 인식'은 문서에서 전문용어가 될 수 있는 언어적 단위를 파악하는 '용어 추출' 과정과 '용어추출' 과정에서 얻어진 용어목록 중 해당분야의 전문용어를 고르는 '전문용어 선택' 과정으로 구성된다. '전문용어 선택' 과정은 용어목록을 전문용어의 특정에 따라 순위화한 후 타당한 전문용어를 파악하는 작업으로 정의된다. 따라서 전문용어 선택 문제는 용어목록의 순위화 작업과 순위화된 목록에서 전문용어와 비전문용어 간의 경계를 인식하는 작업으로 정의된다. 기존의 전문용어 선택 기법은 주로 용어의 빈도수 등과 같은 통계적 특정만을 이용하였다. 하지만 통계적 특정만으로는 효과적으로 전문용어를 선택하기 어렵다. 본 논문의 논제는 전문용어 선택에서 다양한 전문용어의 특정을 고려하고 이들 중 전문용어 선택에서 효과적인 특정을 찾으려는 것이다. 순위화 문제는 다양한 전문용어 특정을 도출하고 이들을 기계학습방법으로 통합하여 해결한다. 경계인식 문제는 전문용어와 비전문용어의 이진 분류 문제로 정의하고 기계학습방법으로 해결한다. 본 논문의 기법은 경계인식측면에서 78-86%의 정확률과 87% -90%의 재현율을 나타내었으며, 순위화 측면에서 89%-92%의 11포인트 평균정확률을 나타내었다. 또한 기존 연구보다 최고 26% 의 성능향상을 보였다.

• 대한소아치과학회지
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• 제23권2호
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• pp.429-449
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• 1996

An objective definition of the anomaly is not avaliable and most investigators define the term differently or fail to describe their criterion. Because dental anomaly may lead to many complications, early detection and diagnosis of dental anomalies are essential steps in the evaluation of the child patient and in treatment planning. These procedures require detailed medical and dental histories, through clinical examination and the use of radiographs. So, this study was designed to find out the prevalence of dental anomalies. The clinical and roentgenographic examination was undertaken for 8,054 children at age from 0 to 15 years and statistical analysis was done. The results were as follows : 1. Among the examined subjects, 2,134 subjects(26.5%) showed dental anomalies. The prevalence of individual dental anomalies were as follows : supernumerary teeth 15.6%, congenitally missing teeth 6.6%, fusion 2.2%, odontoma 0.35%, microdontia 1.2%, macrodontia 0.05%, gemination 0.22%, talon cusp 0.36%, dens evaginatus 0.24%, dens invaginatus 0.15%, dilaceration 0.27%, taurodontism 0.09%, abnormal tooth position 1.7%, natal & neonatal teeth 0.92%, amelogenesis imperfecta 0.01%. 2. Significant correlations between the groups with individual dental anomalies were as follows : between group I and other groups, there was negative correlation, especially group I and group II. And the correlation coefficient between male and female showed differences. 3. For the supernumerary teeth group, the prevalence of male was higher than female(p<0.001). While for the congenitally missing teeth, macrodontia, microdontia, abnormal tooth position group, the prevalence of female was greater than male(p<0.001). 4. For the congenitally missing teeth group, the mandibular primary lateral incisor showed the highest incidence in primary dentiton, while mandibular lateral incisor in permanent dentition. In the mandible(72.5%), this site showed higher prevalence than in maxilla. In the case with deciduous congenitally missing teeth, the prevalence of successive permanent congenitally missing teeth was about 33.9%, the incidence was highest in mandibular lateral incisors. 5. Most supernumerary teeth existed on middle area and showed inverted position and unerupted state. In addition, supernumerary teeth showed higher incidence on maxilla (99.3%). 6. In the case with deciduous fused teeth, the prevalence of successive permanent congenitally missing teeth was 39.9%, while that of successive permanent fused teeth was 2.7%. And the highest rate of the prevalence (40.3%) appeared in fusion of mandibular deciduous lateral incisor and canine. 7. In the case of odontoma, the prevalence was higher in maxilla(78.6%) and anterior region(82.7%) than mandible and posterior region.

• 한국식품과학회지
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• 제22권1호
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• pp.26-32
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• 1990

김치발효 지표로서 미생물군집의 특성을 밝히고자 하였다. 군집크기는 Gram염색법에 의한 현미경직접계수에 의하여 측정하였다. 실온$(15^{\circ}C)$에서 김치발효는 Gram양성균군집, 효모군집 그리고 Gram음성균군집이 순서적으로 천이되었다. Gram양성균군집은 김치를 발효시키는 유산균으로 구성되어 있다. 효모군집의 발달은 김치의 악취를 내는 원인이 되며, Gram음성균군집은 악취와 더불어 김치의 연화작용에 관계된다는 사실이 특정적이었다. 그리고, 외관상의 Gram음성균군은 Gram양성균군집 내의 개체군의 노화와 사멸을 평가하는데 이용될 수 있었다. 특히 저온$(5^{\circ}C)$ 김치발효에서는 효모군집과 Gram 음성균군집이 발달되지 않고 Gram양성균군집만이 발달됨을 알았다. 이 사실로부터 숙성된 김치는 Gram양성균군집에 의하여 만들어짐을 의미한다. 따라서 미생물군집의 발달과 크기는 김치발효의 지표로 이용될 수 있고, 또한 김치숙성도를 수십분 내에 예측하는데 중요한 방법이 될 수 있다.

• 지구물리
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• 제6권1호
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• pp.1-11
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• 2003

측지망 조정에는 여러 가지 기법이 있으나 가장 보편적인 것이 최소자승법이다. 지각 변위 연구 등 지구역학적 연구를 위해 GPS 자료를 처리할 때 일반적으로 두 가지 망조정 기법이 사용되는데 그 중 하나는 자유망 조정법이고 나머지 하나는 제한적망 조정법이다. 이 두가지 기법 중에서 어느 것이 더 지구역학적 목적에 적절한지 결정하기 위해 두 개의 다른 측지망 즉, "이집트의 시나이 측지망"과 "한국의 HGN 측지망"의 데이터를 사용하여 조사하였다. 사용된 두 개의 망은 서로 모양이 다를 뿐만 아니라 지진학적 활동이 다른 지역에 위치해 있다. 이 연구의 결과는 두 기법이 모두 RMS 입장에서 보면 고정밀성을 가지고 있고 큰 차이가 없을을 보인다. 그러나 좌표값 결과를 볼 때 제한적망 고정법이 자유망 조정법에 비해 더 많은 왜곡을 일으켰을 분만 아니라 측지망을 정의하는 고정점이 변하면 좌표값도 다르게 결정되었다. 이러한 왜곡은 측정점의 위치 이동량은 물론 자료처리 결과의 지구역학적 해석에 중요한 역할을 하는 변위요소들의 추정에도 영향을 미친다. 이 연구의 대상 지역에 대해 널리 알려진 지구역학 모델과 두 기법으로부터 얻어진 결과를 비교해 보면, 자유망 조정법의 결과가 이들 모델에 확실히 잘 맞는 반면 제한적망 조정법의 결과는 전혀 맞지 않는것으로 나타났다. 이런 결과를 종합해 볼 때 자유망 조정법이 지구역학적 목적에 더 부합하는 측지망 조정기법이라고 판단된다.

• Journal of Ginseng Research
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• 제39권2호
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• pp.169-177
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• 2015

Background: Ginsenoside Rd (GSRd), one of the most abundant ingredients of Panax ginseng, protects the heart via multiple mechanisms including the inhibition of $Ca^{2+}$ influx.We intended to explore the effects of GSRd on L-type $Ca^{2+}$ current ($I_{Ca,L}$) and define the mechanism of the suppression of $I_{Ca,L}$ by GSRd. Methods: Perforated-patch recording and whole-cell voltage clamp techniques were applied in isolated rat ventricular myocytes. Results: (1) GSRd reduced $I_{Ca,L}$ peak amplitude in a concentration-dependent manner [half-maximal inhibitory concentration $(IC_{50})=32.4{\pm}7.1{\mu}mol/L$] and up-shifted the current-voltage (I-V) curve. (2) GSRd ($30{\mu}mol/L$) significantly changed the steady-state activation curve of $I_{Ca,L}$ ($V_{0.5}:-19.12{\pm}0.68$ vs. $-6.26{\pm}0.38mV$; n = 5, p < 0.05) and slowed down the recovery of $I_{Ca,L}$ from inactivation [the time content (${\zeta}$) from 91 ms to 136 ms, n = 5, p < 0.01]. (3) A more significant inhibitive effect of GSRd ($100{\mu}mol/L$) was identified in perforated-patch recording when compared with whole-cell recording [$65.7{\pm}3.2%$ (n = 10) vs. $31.4{\pm}5.2%$ (n = 5), p < 0.01]. (4) Pertussis toxin ($G_i$ protein inhibitor) completely abolished the $I_{Ca,L}$ inhibition induced by GSRd. There was a significant difference in inhibition potency between the two cyclic adenosine monophosphate elevating agents (isoprenaline and forskolin) prestimulation [$55{\pm}7.8%$ (n = 5) vs. $17.2{\pm}3.5%$ (n = 5), p < 0.01]. (5) 1H-[1,2,4]Oxadiazolo[4,3-a]-quinoxalin-1-one (a guanylate cyclase inhibitor) and N-acetyl-$\small{L}$-cysteine (a nitric oxide scavenger) partly recovered the $I_{Ca,L}$ inhibition induced by GSRd. (6) Phorbol-12-myristate-13-acetate (a protein kinase C activator) and GF109203X (a protein kinase C inhibitor) did not contribute to the inhibition of GSRd. Conclusion: These findings suggest that GSRd could inhibit $I_{Ca,L}$ through pertussis toxin-sensitive G protein ($G_i$) and a nitric oxide-cyclic guanosine monophosphate-dependent mechanism.

• 융합정보논문지
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• 제10권2호
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• pp.184-192
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• 2020

본 연구는 척추측만증 수술에 대한 객관적이고 정량적인 효과 평가 수단으로서 스마트 깔창을 이용한 보행분석 방법(시계열 분석 포함)을 제시한다. 실험 참가자는 척추측만증 환자이며 스마트 깔창을 착용하고 3분 보행검사를 4번(수술전, 수술 후 8일, 16일, 204일), 6분 보행검사를 1번(수술 후 204일) 받았다. 깔창에는 8개의 압력센서, 가속도 및 각속도 센서가 있고, 각각의 측정값을 저장하여 환자의 수술 전후 보행특성(운동역학 및 시공간 변수)을 비교하였다. 분석결과 수술 후 환자의 모든 보행변수가 개선된 것을 알 수 있었고, 6개월 후 추적검사에서 환자의 보행이 더욱 안정된 것을 확인할 수 있었다. 하지만 환자가 오래 걸으면 한쪽 다리의 swing 시간이 다른 쪽에 비해 미세하게 짧은 현상이 다시 나타났는데, 이는 검사를 수행하는 의사의 육안으로는 발견할 수 없는 preclinical한 문제였다. 우리는 이러한 분석 방법을 통해 환자의 개선 정도를 정량적이고 객관적으로 평가할 수 있었고, preclinical한 문제도 찾을 수 있었다. 향후 이러한 분석 방법은 특정 질병의 보행 패턴을 정의하고 감별하여 적절한 치료방법을 결정하는 연구로 이어질 것이다.