• Molecules and Cells
• /
• v.27 no.6
• /
• pp.681-687
• /
• 2009

We examined the effects of synthetic signal peptides, wild-type (WT) and export-defective mutant (MT) of ribose-binding protein, on the conformational changes of signal recognition particle 54 homologue (Ffh) in Escherichia coli. Upon interaction of Ffh with WT peptide, the intrinsic Tyr fluorescence, the transition temperature of thermal unfolding, and the GTPase activity of Ffh decreased in a peptide concentration-dependent manner, while the emission intensity of 8-anilinonaphthalene-1-sulfonic acid increased. In contrast, the secondary structure of the protein was not affected. Additionally, polarization of fluorescein-labeled WT increased upon association with Ffh. These results suggest that WT peptide induces the unfolded states of Ffh. The WT-mediated conformational change of Ffh was also revealed to be important in the interaction between SecA and Ffh. However, MT had marginal effect on these conformational changes suggesting that the in vivo functionality of signal peptide is important in the interaction with Ffh and concomitant structural change of the protein.

• Mass Spectrometry Letters
• /
• v.2 no.3
• /
• pp.69-72
• /
• 2011

Defective synthesis of the steroid hormones by the adrenal cortex has profound effects on human development and homeostasis. Due to the time-consuming chromatography procedure combined with mass spectrometry, the matrix-assisted laser desorption ionization method coupled to the linear ion-trap tandem mass spectrometry (MALDI-LTQ-MS/MS) was developed for quantitative analysis of 10 adrenal steroids in human serum. Although MALDI-MS can be introduced for its applicability as a high-throughput screening method, it has a limitation on reproducibility within and between samples, which renders poor reproducibility for quantification. For quantitative MALDI-MS/MS analysis, the stable-isotope labeled internal standards were used and the conditions of crystallization were tested. The precision and accuracy were 3.1~35.5% and 83.8~138.5%, respectively, when a mixture of 10 mg/mL ${\alpha}$-cyano-4-hydroxycinnamic acid in 0.2% TFA of 70% acetonitrile was used as the MALDI matrix. The limit of quantification ranged from 5 to 340 ng/mL, and the linearity as a correlation coefficient was higher than 0.988 for all analytes in the calibration range. Clinical applications include quantitative analyses of patients with congenital adrenal hyperplasia. The devised MALDI-MS/MS technique could be successfully applied to diagnosis of clinical samples.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
• /
• v.21 no.3
• /
• pp.27-34
• /
• 2007

The rotor faults of induction motors may cause bad effects on the performance of the induction motor. This paper proposes the detecting technique of these faults by analyzing the waveform of the induced current and voltage of search coil using numerical analysis and the experiment. Several defective rotor bars are simulated to analyze the fault conditions-broken bars and high resistance of rotor bars. In order to prove the usefulness of the proposed method, we made an prototype experimental apparatus. The waveform of the induced voltages in search coil has the obvious characteristics and it is easy to differentiate the normal rotor from the abnormal one. The experimental results show that the proposed method is useful to detect the rotor fault conditions.

• Journal of Construction Engineering and Project Management
• /
• v.3 no.3
• /
• pp.35-41
• /
• 2013

All infrastructure projects are said to be inter-dependent, uncertain and labour-intensive in nature. There is no exception for building services sub sector. For a real time project such as 'The construction, extension and refurbishment of Employees' State Insurance Corporation (ESIC) Hospital at Tirupathy, India with total area of 45,000 square feet at an estimated cost of 1100 million rupees, a generic process model is developed to simulate the effect of set of identified variables on construction project. The 'Stocks and Flows' of dynamic model affords relevant insights to project managers, who apply this knowledge when designing better performance through more appropriate project planning. It is concluded from the model-based approach that building services works can be improved through specific better focussed managerial efforts, such as an increasing coordination effectiveness at the planning stage, clarifying prerequisite conditions prior to installations. Otherwise, pending works arising from work clashes can lead to knock-on effects resulting in productivity constraints and pressures, as well as more rework and demolition. Current study reveals that the model enables deep insight into various interdependent processes, their by improving construction performance levels, by addressing the dynamics of design errors and defective works, and recovering delayed schedule.

• The Transactions of the Korean Institute of Electrical Engineers C
• /
• v.55 no.4
• /
• pp.189-198
• /
• 2006

In order to improve the reliability of stator windings for hydro generators, it is necessary to detect the defective point and life assessment of the high voltage windings. Especially the on-line partial discharge (PD) test provides the ability to monitor effects, such as slot discharge, internal discharge, and end-winding discharge without interrupting generators, this method has been proven the major testing technology in high voltage rotating machines nowadays. The purpose of this paper is to describe the method of the on-line PD measurement on stator windings for hydro-generator with 13 [kV] class ceramic coupler (CC), on-line PD measuring system, terminal box, and index parameters. Also, the developed PD measuring system and 13 [kV] class CC were installed Daechung-dam generator #2. It was found that this installed sensor and system had good electrical characteristics to detect PD activity during the operating condition with its detection frequency band is between several and several tens MHz.

• Korean Journal of Clinical Laboratory Science
• /
• v.49 no.4
• /
• pp.495-497
• /
• 2017

Thalassemia major is a genetic disorder with a defective synthesis of either the alpha or the beta chain of hemoglobin A. Blood transfusion is crucial for the survival in these patients. Unfortunately, endocrine dysfunction is a very common complication in these patients and is principally due to excessive iron overload as a result of frequent blood transfusions. Although regular blood transfusion may increase life expectancy, disturbances in growth and pubertal development, abnormal gonadal functions, impaired thyroid, parathyroid and adrenal functions, diabetes, and disorderly bone growth are common side effects. We hereby present a case of a 23-year-old, unmarried woman with beta thalassemia major presenting with primary amenorrhea, poor development of secondary sexual character, and short stature. Thorough history, clinical examination, and laboratory investigation, including dynamic function test (insulin tolerance test) were conducted. These tests confirmed that she had multiple endocrinopathies, including hypogonadotropic hypogonadism, growth hormone deficiency, and subclinical adrenal insufficiency, which were caused by iron overload. She required hormone replacement therapy. Early recognition of possible deficiencies in hypothalamo-pituitary-end organ hormones caused by iron overload in thalassemia patients that undergo frequent blood transfusion procedures is essential. Appropriate treatments, including transfusion regimen and chelation therapy, as well as specific treatment of each complication are the crucial for the successful management and improvement of quality of life these patients.

• Korean Journal of Environmental Biology
• /
• v.20 no.1
• /
• pp.35-39
• /
• 2002

The significance of cell ploidy and repair ability for the radioprotective efficiency of cysteamine was studied in DNA repair - proficient and repair - deficient yeast cells irradiated $^{60}C0\;\gamma-rays.$ Results have been obtained for the cell survival of two groups of yeasts-diplont and haplont cells, both in haploid and diploid states. For diploid Saccharomyces cerevisiae yeast cells, the correlation between the radio-protective action of cysteamine and the cell repair capacity was demonstrated. Such a correlation was not clearly expressed for haploid yeast cells. In addition, evidence was obtained indicating that the degree of the radioprotective action was independent of the number of chromosome sets in haplont yeast Pichia guilliermondii cells and in some radiosensitive mutants defective in the diploid-specific recovery. It is concluded on this basis that the radioprotective action may involve the cellular recovery process, which may be mediated by a recombination-like mechanism, for which the diploid state is required. The results obtained clearly show that the radioprotective effect was dependent on DNA repair status and indicate that the mechanism of the radioprotective action may be realized on the level of primary radiation damage production as well as on the level of postradiation recovery from potentially lethal radiation damage.

• The Transactions of the Korean Institute of Electrical Engineers C
• /
• v.55 no.5
• /
• pp.223-230
• /
• 2006

The reliability of imprint patterns molded by stamps for industrial application of nanoimprint lithography (NIL), is an important issue. Usually, defects can be produced by incomplete filling of negative patterns and the shrinkage phenomenon of polymers in conventional NIL. In this paper, the patterns that undergo a varied temperature or varied pressure period during the thermal NIL process have been investigated, with the goal of resolving the shrinkage and defective filling problems of polymers. The effects on the formation of polymer patterns in several profiles of imprint processes are also studied. Consequently, it is observed that more precise patterns are formed by the varied temperature (VT-NIL) or varied pressure (VP-NIL). The NIL (VT-NIL or VP-NIL) process has a free space compensation effect on the polymers in stamp cavities. From the results of the experiments, the polymer's filling capability can be improved. The VT-NIL is merged with the VP-NIL for the better filling property. The patterns that have been imprinted in the merged NIL are compared with the results of conventional NIL. In this study, the improvement in the reliability for results of thermal NIL has been achieved.

• Journal of the Korea Fashion and Costume Design Association
• /
• v.19 no.1
• /
• pp.121-134
• /
• 2017

The purpose of this study is to investigate the effect of educational satisfaction on the school conversion intention according to the quality of university education service. To survey the perception of students majoring in Department of Beauty on both colleges and universities of beauty education, we distributed 600 questionnaires and utilized them as final analysis data except for 60 unreturned or defective papers. As a result of t-test and one-way ANOVA, there were differences among university education service quality, education satisfaction and school transfer intention. In addition, the effect of the quality of education service on the education service, the effect of the quality of education service on the school conversion intention, and the quality of the university education service influenced the school conversion intention. The results of this study suggest that, for the development of the beauty education, priority should be given to the facilities and equipments related to the beauty education as well as the importance of leadership and professional teachers and instructors.

• Restorative Dentistry and Endodontics
• /
• v.36 no.4
• /
• pp.271-279
• /
• 2011

In clinic, esthetic restoration of a defective natural tooth with composite resin is challenging procedure and needs complete understanding of the color of tooth itself and materials used. The optical characteristics of the composites are different because the chemical compositions and microstructures are not same. This review provided basic knowledge of the color and the color measurement devices, and analyze the color of the natural tooth. Further, the accuracy of the shade tab, color of the composite resins before and after curing, effect of the water, food and bleaching agent, and translucency, opalescence, and fluorescence effects were evaluated.