• Title/Summary/Keyword: de-construction

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Overexpression and Characterization of Eukaryotic Peptide Hormone Precursors in E. Coli. (대장균에서 진핵세포 펩타이드 호르몬 전구물질의 대량생산과 특성규명)

  • 홍승환
    • The Korean Journal of Zoology
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    • v.33 no.3
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    • pp.303-309
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    • 1990
  • In order to have a handle on the availability of eukarvotic peptide hormone precursors, a cDNA encoding angler fish prepro-SRIF I was manipulated so that it can be produced in large quantity from heterologous E. coli cells. Using T7 overexpression system, fusion constructs between the T7 phage coat protein Sl0 and the prepro-SRIF were made and modified as desired. From the host E. coli strain, BL21 DE3, harboring these plasmid constructs, three different SRIF related polypeptides were expressed in large amount and characterized. The results confirm the exact construction and authenticity of the overexpressed proteins from E. coli cells. The importance of this heterologous overexpression in hard to get peptide hormone precursors as well as the suitability of the target peptide hormone SRIF for this approach are discussed.

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A Study on Theo van Doesburg's Plastic Experiments with Colored Planes (반 두즈버르그의 색면 조형 실험에 관한 연구)

  • Park, Young-Kyung
    • Korean Institute of Interior Design Journal
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    • v.16 no.3 s.62
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    • pp.30-37
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    • 2007
  • This research is to find out the characteristics and significance of the plastic experiments with colored planes in Theo van Doesburg's works. Based on the analysis, the following results have been obtained. First, Thea van Doesburg's plastic experiments with colored planes started from the paintings in 1916-1918. series and series were made of the ion of still life or human motion. These paintings were intended to make effects of rhythm, acceleration and simultaneity. Second, he colored the outer and inner architectural surface with primary colors. The expression of visual. rhythm on the color design for De Vonk, Districts Housing Projects VIII & IX in Spangen, Multi-Housing in Oosterstraat, Friesian Housing, Landbouw Winter School, and University Hall in Amsterdam embodied the concept of painting-in-architecture. Third, the field of these experiments were extended into the architectural space. As a results, $H\hat{o}tel$ Particulier and Maison d'Artiste was shown on the Architectural Exhibition in Paris in 1923. The colored planes were used as a construction material. They generated the dynamic space in architecture. Fourth, through the pictorial works like Architectural Analysis, Contra-Composition, Simultaneous Composition or Simultaneous Contra-Composition, Tesseract and Aubette Cinema-Dancing Hall, he created the concepts of simultaneous integration including nature and the environments as well as space-time in the architecture.

A Study on the Transition of Spatial Structure in Libraries with Special Reference to Rhizome and Hypertext (리좀과 하이퍼텍스트 관점에서 본 도서관 공간구조의 이해)

  • Choi, Yoon-Kyung;Kim, Min-Jung
    • Korean Institute of Interior Design Journal
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    • v.15 no.6 s.59
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    • pp.111-119
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    • 2006
  • The spatial property of contemporary library is now rapidly changing through the spatial expansion of knowledge and information, the reduction of information storing facility, the variation of approaching methods by digital shift and the transition of social recognition as a cultural facility. Also the spatial characteristics with referring characters have developed such as, decentralization, de-construction, de-boundary, individual space, erasing of boundary, flow of space which extends infinitely. The main process of library origination, the systematic classification, and the storage system concluding with the demand and value of the information by changing social demands and the role of the widest ranged facility. And 5 themes, such as, hierarchy, center, storage, boundary, and symbol, as a changed spatial concept and analyzed in the case of library plans and libraries which are actually built. The significant purpose of this research is to propose that rhizomatous intellectuality and hypertext could be a theoretical background of the contemporary architecture and could be a viewpoint of the transition of spatial structure in libraries. A future library should have spatial property embracing various social changes and needs and for this respect, it is necessary to approach and analyze through the architectural explication from diverging points of view.

Damage-based stress-strain model of RC cylinders wrapped with CFRP composites

  • Mesbah, Habib-Abdelhak;Benzaid, Riad
    • Advances in concrete construction
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    • v.5 no.5
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    • pp.539-561
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    • 2017
  • In this study, the effects of initial damage of concrete columns on the post-repair performance of reinforced concrete (RC) columns strengthened with carbon-fiber-reinforced polymer (CFRP) composite are investigated experimentally. Four kinds of compression-damaged RC cylinders were reinforced using external CFRP composite wraps, and the stress-strain behavior of the composite/concrete system was investigated. These concrete cylinders were compressed to four pre-damaged states including low -level, medium -level, high -level and total damage states. The percentages of the stress levels of pre-damage were, respectively, 40, 60, 80, and 100% of that of the control RC cylinder. These damaged concrete cylinders simulate bridge piers or building columns subjected to different magnitudes of stress, or at various stages in long-term behavior. Experimental data, as well as a stress-strain model proposed for the behavior of damaged and undamaged concrete strengthened by external CFRP composite sheets are presented. The experimental data shows that external confinement of concrete by CFRP composite wrap significantly improves both compressive strength and ductility of concrete, though the improvement is inversely proportional to the initial degree of damage to the concrete. The failure modes of the composite/damaged concrete systems were examined to evaluate the benefit of this reinforcing methodology. Results predicted by the model showed very good agreement with those of the current experimental program.

Numerical simulation of the constructive steps of a cable-stayed bridge using ANSYS

  • Lazzari, Paula M.;Filho, Americo Campos;Lazzari, Bruna M.;Pacheco, Alexandre R.;Gomes, Renan R.S.
    • Structural Engineering and Mechanics
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    • v.69 no.3
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    • pp.269-281
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    • 2019
  • This work addresses a three-dimensional nonlinear structural analysis of the constructive phases of a cable-stayed segmental concrete bridge using The Finite Element Method through ANSYS, version 14.5. New subroutines have been added to ANSYS via its UPF customization tool to implement viscoelastoplastic constitutive equations with cracking capability to model concrete's structural behavior. This numerical implementation allowed the use of three-dimensional twenty-node quadratic elements (SOLID186) with the Element-Embedded Rebar model option (REINF264), conducting to a fast and efficient solution. These advantages are of fundamental importance when large structures, such as bridges, are modeled, since an increasing number of finite elements is demanded. After validating the subroutines, the bridge located in Rio de Janeiro, Brazil, and known as "Ponte do Saber" (Bridge of Knowledge, in Portuguese), has been numerically modeled, simulating each of the constructive phases of the bridge. Additionally, the data obtained numerically is compared with the field data collected from monitoring conducted during the construction of the bridge, showing good agreement.

The Social Identity Dynamics of Soft Power Narrative Influence: Great Power Diplomatic Bargaining Leverage Amidst Complex Interdependence

  • DeDominicis, Benedict E.
    • International Journal of Advanced Culture Technology
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    • v.10 no.3
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    • pp.127-145
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    • 2022
  • Vaccine diplomacy is a manifestation of competition for political influence among great powers amidst the Covid-19 pandemic's blatant illustration of ineluctable interdependency across the global community. The reinforcement of trends bolstering global polity construction intensify concomitantly with nationalist populist value and attitude expressions increasing political polarization. The interdependency graphically illustrated in the Cold War-era's mutual assured destruction incentivized competition into indirect competitive intervention in the internal politics of third actors. Indirect international influence contestations included extended, de facto challenge competitions to generate soft power on behalf of the victor, e.g., the space race. The Covid-19 pandemic has intensified this competition to offer alternative development models while intense domestic political polarization undermines the mobilizational capacities for achieving sustainable development. In contrast to multinational and multiethnic states, nation states have an inherent mobilizational advantage because of the enhanced control capabilities available to the authorities without emphasizing coercion. Control through Gramscian hegemonic mechanisms is more readily feasible in nation states through the greater feasibility of commodification of social relations by states authorities regulating and channeling social competition to encourage social mobility and creativity. The regulation of the so-called private sector serves to manage and contain social competition while channeling it to develop the institutional capacities for control and allocation of developing societal human resources. It enhances developed state control mechanisms and international influence capacities. The appeal of offers of aid and assistance to the so-called developing world becomes ever more urgent amidst Anthropocene crises including its most recent, current Covid-19 pandemic disaster.

Novel green composite material manufactured by extrusion process from recycled polypropylene matrix reinforced with eucalyptus fibres and granite powder

  • Romulo Maziero;Washington M. Cavalcanti;Bruno D. Castro;Claudia V. Campo, Rubio;Luciano M.G. Vieira;Tulio H. Panzera;Juan C. Campos Rubio
    • Advances in materials Research
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    • v.12 no.2
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    • pp.119-131
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    • 2023
  • The development of sustainable composites materials, from recycled polymeric materials and waste from the wood industry and stone processing, allows reducing the volume of these by-products, minimizing impacts on health and the environment. Nowadays, Polypropylene (PP) is the most recycled polymer in industry, while the furniture industry has increasingly used timber felled from sustainable forest plantations as a eucalypt. The powder tailing from the ornamental stone extraction and processing industry is commonly disposed of in the environment without previous treatment. Thus, the technological option for the development of composite materials presents itself as a sustainable alternative for processing and manufacturing industries, enabling the development of new materials with special technical features. The results showed that powder granite particles may be incorporated into the polypropylene matrix associated with short eucalyptus fibres forming green hybrid composites with potential application in structural engineering, such as transport and civil construction industries.

Dissipative Replaceable Bracing Connections (DRBrC) for earthquake protection of steel and composite structures

  • Jorge M. Proenca;Luis Calado;Alper Kanyilmaz
    • Steel and Composite Structures
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    • v.46 no.2
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    • pp.237-252
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    • 2023
  • The article describes the development of a novel dissipative bracing connection device (identified by the acronym DRBrC) for concentrically braced frames in steel and composite structures. The origins of the device trace back to the seminal work of Kelly, Skinner and Heine (1972), and, more directly related, to the PIN-INERD device, overcoming some of its limitations and greatly improving the replaceability characteristics. The connection device is composed of a rigid housing, connected to both the brace and the beam-column connection (or just the column), in which the axial force transfer is achieved by four-point bending of a dissipative pin. The experimental validation stages, presented in detail, consisted of a preliminary testing campaign, resulting in successive improvements of the original device design, followed by a systematic parametric testing campaign. That final campaign was devised to study the influence of the constituent materials (S235 and Stainless Steel, for the pin, and S355 and High Strength Steel, for the housing), of the geometry (four-point bending intermediate spans) and of the loading history (constant amplitude or increasing cyclic alternate). The main conclusions point to the most promising DRBrC device configurations, also presenting some suggestions in terms of the replaceability requirements.

Durability Characteristics of Limestone Powder added Concrete for Environment-Friendly Concrete (석회석미분말을 첨가한 친환경 시멘트콘크리트의 내구 특성)

  • Choi, Woo Hyeon;Park, Cheol Woo;Jung, Won Kyung;Jeon, Beom Joon;Kim, Gyu Seon
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.16 no.5
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    • pp.59-67
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    • 2012
  • During the manufacturing of Portland cement, CO2 gas is also necessarily produced through both decarbonation of calcium carbonate and kiln burning. By partially replacing the Portland cement with limestone powder, which is an inert filler in a concrete mixture, CO2 consumption can be reduced in a construction field. This study is to investigate the fundamental durability characteristics of limestone powder added concrete. Experimental variable was the replacement ratio of limestone powder from 0% to 25% with 5% increment. Durability characteristics were investigated by resistance to freeze-thaw, alkali-silica reaction and de-icing chemical in addition to the properties of fresh concrete. From test results, it was observed that the addition of limestone powder did not significantly affect the resistance to freeze-thaw reaction and de-icing chemical. The addition of limestone powder reduced the occurrence potential of alkali-silica reaction by reducing an alkali content in Portland cement.

Change of Ammonia Consumption with Gas Turbine Output in DeNOx System for a 580 MW Combined Cycle Power Plant (580 MW급 복합발전소 탈질설비에서 가스터빈 출력에 따른 암모니아 소모량 변화)

  • Jang, Yong-Woo;Yoo, Ho-Seon
    • Plant Journal
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    • v.15 no.3
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    • pp.23-28
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    • 2019
  • In this study, ammonia consumption by gas turbine output was adjusted to find out the amount of ammonia consumption that complies with the enhanced Air Quality Preservation Act and internal regulation emission standards in SCR type DeNOx System for a 580 MW Sejong Combined Cycle Power Plant. For measurements, the gas turbine output was varied to 50, 99, 149, 198 MW and ammonia consumption was adjusted with the combustion gas and ammonia supply conditions fixed at each stage. When the emission limit were change from 10 ppm to 8 ppm, ammonia consumption was increased from 78, 93, 105, 133 kg/h to 89, 113, 132, 176 kg/h. The increase rate of ammonia consumption was 14, 22, 26, 32% per output category compared to the 10 ppm emission limit, which was shown to increase as output increased.