• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.3
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• pp.343-348
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• 2015

Soybean is one of the important food crop around the world. Especially in East Asia, it is the main ingredient for traditional food like soy sauce and soy paste. The double cropping system including soybean following onion, Chinese cabbage, and potato is widely adopted in Southern region of Korea. In this system, sowing date of second crop (soybean) can be delayed depending on first crops' growth period and weather condition. When planting date is delayed it is known that soybean yield is declined because of shorter vegetative growth period and earlier flowering induced by warm temperature and changes in photoperiod. The objective of this study was to determine soybean growth and yield responses as plant populations at late planting date. Field experiment was conducted at Department of Functional Crop, National Institute of Crop Science, RDA located in Miryang, Gyeongsangnam-Do for two years ('13-'14) in upland field with mid-late maturity cultivar Daewon. A split-plot block design was used with three replications. Main plots were three sowing dates from June 20 to July 20 with 15 days intervals, and subplots were 4 levels of planting densities. Data of maturity (R8) was recorded, yield components and yield were examined after harvesting. Experimental data were analyzed by using PROC GLM, and DMRT were used for mean comparison. Optimum planting population for maximizing soybean yield in late planting which compared with standard population. In mid-June planting, higher planting density causes increased plant height and decreased diameter which lead to higher risk of lodging, however, reduced growth period due to late planting alleviated this problem. Therefore higher seeding rates can provide protection against low seedling emergence caused by late planting in this region.

• Horticultural Science & Technology
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• v.16 no.1
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• pp.27-29
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• 1998

Artificial pollination (AP) and gibberellin A4+7 plus benzyladenine (promalin) were applied alone and together. AP was applied at 10% flowering time with 'Senshu' pollen (Malus domestica cv. Senshu). 12mg/L promalin was applied at 0, 10 and 20 days after falling of central flowers, respectively. In promalin treatment with or without AP application methods, fruit length, weight and length/diameter (L/D) were higher than those of control and AP. However, in AP and AP+ promalin application, the number of seeds and seed weight were higher than those of control and promalin. In AP+ promalin treatment, 78.6% fruits showed their uniform fruit shape and so significantly enhanced fruit uniformity compared to other treatments. Also cortex and core thickness of fruits were greater at the apex than that of other treatment. Fruit with L/D ratio over 0.87 were highly produced by applications of AP+promalin and promalin than control and AP only. 'Fuji' apples in good shape can be produced by using of AP + promalin together.

• Journal of The Korean Society of Grassland and Forage Science
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• v.5 no.2
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• pp.106-110
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• 1985

This trial was carried out to find out the optimum ridge and seeding rate for the seed production of Festuca arundinacea S. (variety Alta). It was composed of three ridge level (12, 18 and 24cm) and four seeding rates (10, 15, 20 and 25kg/ha) in a split-plot design with 4 replications. The experiment lasted from September 1979 to October 1981. 1. The beginning of heading state was May 12 and the date of full flowering stage was June 2. The optimum stage for the seed harvesting of Festuca arundinacea was about 43 days after beginning of heading stage. 2. The number of panicles were 157 per square meter from the best treatment 18cm ridge with 15kg seed/ha. And the 1000 grain weight was 2.79g for the treatment 12cm ridge with 10kg seed/ha. 3. The average seed yield for two years was 681kg/ha and the largest 831kg/ha for the treatment 18cm ridge with 25kg seed/ha. 4. the average germination rate of the harvested seed was 90.7 percent, and it showed a little higher germination rate from the lower level of seeding rate. 5. The average total DM production with two cuttings of aftermath was about 6715kg/ha. According to the increasing seeding rate and narrowing the ridge, DM yield was higher.

• BMB Reports
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• v.39 no.3
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• pp.297-303
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• 2006

As lipolytic enzymes, GDSL lipases play an important role in plant growth and development. In order to identify their functions and roles, the full-length cDNA of a GDSL lipase gene, designated BnLIP2, was isolated from Brassica napus L. BnLIP2 was 1,300 bp long, with 1,122 bp open reading frame (ORF) encoding 373 amino acid residues. Sequence analysis indicated that BnLIP2 belonged to GDSL family. Southern blot analysis indicated that BnLIP2 belonged to a small gene family in rapeseed genome. RT-PCR analysis revealed that BnLIP2 was a tissue-specific expressing gene during reproductive growth and strongly expressed during seed germination. BnLIP2 expression could not be detected until three days after germination, and it subsequently became stronger. The transcript of this gene was deficient in root of seedlings growing at different stages. When juvenile seedlings were treated by methyl jasmonate (MeJ), salicylic acid (SA) and naphthalene acetic acid (NAA), BnLIP2 expression could not be induced in root. Our study implicates that BnLIP2 probably plays an important role in rapeseed germination, morphogenesis, flowering, but independent of root growth and development.

• Journal of Applied Biological Chemistry
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• v.55 no.4
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• pp.227-234
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• 2012

We observed that oil began to accumulate at 25 seed days after flowering (DAF) and reached the maximum potential at 35 seed DAF of oilseed rape, and the greatest weight of 100 seeds was obtained at 35 seed DAF. To survey a broad analysis of gene expression in developing embryos of Brassica napus, the Bn 300k microarray have been constructed. The Bn 300k Microarrary was designed from 80,696 unigenes clustered from 543,448 ESTs and 780 cDNA at NCBI. These arrays have been hybridized in a series of experiments with probes derived from seeds and leaf of B. napus. Approximately 8.5% of the 7,000 genes were expressed as ratios 2-fold higher in seed (25 DAF) than leaves and 0.4% at ratios 10. Also we observed that storage and cell differentiation-related genes were highly expressed at 10 DAF, whereas energy-related genes including fatty acid metabolism were increased up depending on seed maturation using Microarray, which was confirmed by reverse transcriptase polymerase chain reaction. These results suggest that B. napus arrays provide a very useful data set of seed-specific expression that can be further analyzed by examination of the promoter regions of these genes and help our understanding of the complex regulatory network in developing seeds.

• Journal of Bio-Environment Control
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• v.14 no.1
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• pp.15-21
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• 2005

This experiment was carried out to investigate the effect of ridge direction (south-north) on temperature and light intensity on early growth of oriental melon under east-west oriented vinyl house cultivation. The air-temperature of minimum between north and south-ridge in the tunnel of vinyl house was $12.5^[\circ}C\;and\;11.3^{\circ}C$ and that of maximum between north and south-ridge was $36.7^{\circ}C\;and\;34.7^{\circ}C$, respectively. The minimum and maximum air-temperature of north-ridge in the tunnel of vinyl house on Feb. 15 were $12^{\circ}C\;and\;2.0^{\circ}C$ higher than those of south-ridge, respectively. The intensity of daylight between south and north-ridge was similar during 9:00-10:30 a.m., that of south-ridge was higher than north-ridge during 10:30-11:30 a.m. and that of north-ridge was higher than south-ridge during 11:30-17:30. The plant growth after 55 days of planting on the north-ridge was prominent cultivation compared to south-ridge. The female flowering and first harvesting day were earlier in north-ridge than in south ridge. The marketable fruits rate and yields (kg/10a) were $6.7\%$ and 218kg higher in north-ridge cultivation than south-ridge, respectively. Differences of marketable fruit rate and yield (kg/10a) in each ridge were significant.

• Applied Biological Chemistry
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• v.34 no.2
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• pp.134-141
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• 1991

In order to investigate the changes and characteristics of biochemical metabolic substances of soybean tissue culture during the cultural period, immature cotyledons were detached form the plant on 15th days after flowering and cultured in vitro for 3 weeks. The cultures were classified into embryogenic(EC) and non-embryogenic callus(NEC). A part of the EC lines were subcultured for another 3 weeks and classified into root forming(RFC), and shoot forming cultures(SFC). Another part of the EC lines were used for isolation of protoplasts, which were subsequently cultured in vitro for 4 weeks. The cultures were classified into embryogenic(PEC) and non-embryogenic callus(PNEC) derived from the protoplasts. The cultures of EC and PEC lines showed higher phenylalanine content and lower methionine content than those of NEC and PNEC. At organ differentiation stage, both cultures showed the content of aspartic acid decreased, while the other amino acids increased as a whole. The protein pattern analysis of the cultures revealed that EC and NEC lines contained distinctive polypeptides, with mass of ca. 18KD for EC and ca. 22KD for NEC respectively. The EC and PEC lines also showed high activity of peroxidase isozyme A(piA), while the RFC and SFC lines showed that of peroxidase isozyme B(piB).

• Korean Journal of Environmental Agriculture
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• v.34 no.4
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• pp.288-293
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• 2015

BACKGROUND: This study was conducted to investigate the effect of the unexpected early loss of leaves on a newly-bred kiwifruit on the regrowth of axillary buds the current season and the early growth and development the following year.METHODS AND RESULTS: The vines were defoliated on Jul. 18, Aug. 16, and Sep. 17 in 2012 and on Jul. 16, Aug. 13, and Sep. 12 in 2013. The vines were defoliated 0 (control), 50, and 100% of the total number of leaves on a vine. The regrowth of axillary buds at 30 days after defoliation increased in proportion to defoliation degrees regardless of the defoliated time. Defoliation the previous season did not influence percent budbreak the next season. Percentage of floral shoots of the control vines was 27.4%, each bearing 2-3 flowers. In those vines defoliated 100% in August and September, however, percent floral shoots and number of flowers significantly reduced.CONCLUSION(S): Defoliation in July, August, and September didnot affect percent budbreak the following year regardless of degrees of defoliation. A 100% defoliation in August and September significantly reduced flowering the following year compared to the control; that in August resulted in no floral buds at all.

• Journal of Plant Biology
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• v.35 no.3
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• pp.185-190
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• 1992

Changes in activity of gibberellin $3{\beta}-hydroxylase$ which converts $[^3H]GA_{20}\;to\;GA_1$ were studied during seed maturation using partially purified enzyme preparations of two cultivars, Kentucky Wonder (normal) and Masterpiece (dwarf) of Phaseolus vulgaris. The specific activity of $3{\beta}-hydroxylase$ per seed reached maximum at 21 days after flowering and subsequently decreased during seed maturation in both cultivars. The ratios of conversion of $[17-^{13}C,\;^3H_2]GA_{20}\;to\;GA_1.\;GA_5,\;and\;GA_6$ by the same amount of $3{\beta}-hydroxylase$ were almost identical. Epoxidation of $GA_5\;to\;GA_6$ is also catalyzed by the partially purified $3{\beta}-hydroxylase$ preparation(Kobayashi et aI., 1991) and the conversion was inhibited by the substrates of $3{\beta}-hydroxylase$. These results suggest that the same enzyme might catalyze $3{\beta}-hydroxylase{\;}of{\;}GA_{20}\;to\;GA_1$ and epoxidation of $GA_5\;to\;GA_6$..

• Journal of Bio-Environment Control
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• v.24 no.2
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• pp.106-112
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• 2015

The objective of this study was to examine the growth reaction of strawberry plants to the mixed red and blue LED sources and their installation method. The artificial light sources were : LED PAR(PPFD $2{\sim}4{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$), LED BAR(PPFD $100{\sim}120{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and incandescent(PPFD $2{\sim}4{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) lamp. The lighting treatment was started at the first cluster flowering period as a night breaking lighting and was applied during 3 hours, between 22:00 and 01:00 every day. Plant height and leafstalk length were longer in plants treated with incandescent lamp, where as fresh and dry weight of shoot were heavier in LED PAR compared to incandescent lamp treatment. LED PAR treatment also resulted in the largest leaf area, chlorophyll content was increased by $0.36mg{\cdot}g^{-1}$ after 60 days from the starting of the artificial lighting. According to the experimental results application of 16W LED PAR lamps and W-type installation method can improve light environment in strawberry lighting culture.