• Research in Plant Disease
• /
• v.25 no.4
• /
• pp.220-225
• /
• 2019

During the year 2018, the symptoms of bunch rot on Shine Muscat (Vitis vinifera L.) were observed in Kimcheon-si, Gyeongbuk province in Korea. The disease appears on the Shine Muscat as a black rot due to prolific fungal sporulation after it has invaded into the Shine Muscat which look completely empty and dryness. Colonies of these fungi are present on the Shine Muscat skin from fruit setting and increase in amount from early season to harvest, while become peak at ripening stage. To isolate the causal agent, small fragments (2 to 3 mm) of decayed tissue from the lesion margin were placed onto potato dextrose agar (PDA) plates. Fungal colonies on PDA produced dense white aerial mycelium and then covered with dark black conidial heads. These heads were large and radiate, and vesicles were globose (2.12-32.0×2.0-3.1 ㎛). Based on morphological and cultural characteristics, this fungus was identified as Aspergillus tubingensis. To confirm its identity, the internal transcribed spacer, β-tubulin, and RNA polymerase II was sequenced for molecular identification. BLAST search indicated 99% identity with A. tubingensis. The pathogenicity test on healthy grape of Shine Muscat produced bunch rot, as the original symptoms. To select effective fungicides for the control of brunch rot, an in vitro antifungal activity of seven fungicides were evaluated against the growth of A. tubingensis. Five fungicides (dipenoconazole, tebuconazole, metconazole, iminoctadine, and captan) exhibited significantly strong suppression of the mycelial growth of A. tubingensis.

• Korean Journal of Plant Tissue Culture
• /
• v.24 no.1
• /
• pp.57-61
• /
• 1997

We investigated changes in the superoxide dismutase (SOD) activity and SOD isoenzyme pattern in suspension cultures of tomato (Lycopersicon esculentum), which were compared with those of intact tomato plants. two grams (fr wt) of cells subcultured at 15-day intervals were inoculated into 50 mL MS medium containing l mg/L 2,4-D and 30 g/L sucrose in a 300 mL flask and maintained at $25^{\circ}C$ in the dark (100 rpm). The cell growth reached a maximum at 20 days after subculture (DAS), followed by a rapid decrease with further cultures. The cell colour changed from white to black from 23 DAS. The intracellular SOD activity (units/g cell dry wt) was significantly increased from 23 DAS and reached a maximum at 28 DAS (52,400 units), followed by a decrease with further cultures, whereas the extracellular SOD activity showed a maximum at 25 DAS (27,800 units/50 mL medium). The total SOD activity per flask showed a maximum at 25 DAS (35,700 units), in which the extracellular SOD activity occupied about 75%. The tomato cultured cells had four SOD isoenzymes and their patterns were well correlated with SOD activity without a qualitative change during the cell cultures. The intact tomato plants had an additional CuZnSOD isoenzyme, showing the different isoenzyme patterns from cultured cells.

• Korean Journal of Medicinal Crop Science
• /
• v.7 no.3
• /
• pp.155-161
• /
• 1999

This study was carried out to develop the propagation system using in vitro induced- microtubers of yams (Dioscorea alata L.). Effects of kinds of media, mineral composition, sucrose concentration (0, 2, 4, 6, 8, 10%), photoperiod (0, 8, 12, 16, 24h), and growth regulators (NAA, IAA, ZR, JA-Me, ABA) on the development of microtubers, roots, and shoots in nodal stem segment cultures of D. alata L. were evaluated. Microtuberization in nodal stem segment occurred on all the media supplemented with growth regulator and sucrose. Among basic media, 1/2MS medium was the best in microtuber induction. NAA was shown to be the most effective among the growth regulators. Optimal NAA concentration was 1mg/l. The microtuberization was the highest at the concentration of 6% sucrose. When the nodal stem segment were cultured under darkness, the tuberization was increased markedly compared to those cultured under light condition. It was also noticeable that the culture in medium with NAA produced only microtubers and roots, but no shoots, in nodal segments. In this study, the optimal medium composition for microtuberization in nodal stem segment was found to be 1/2MS medium supplemented with 1mg/l NAA and 6% sucrose under dark condition at $25^{\circ}C$.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.34 no.8
• /
• pp.1364-1377
• /
• 2010

Although the human body is a biological subject with definite and distinctive physical features, its actualization and perception differs among societies. The aesthetics of the human body are based on diverse cultural perceptions that must be considered prior to design development. This study establishes the foundations of newly adopted concepts of beauty that are presumed to have been established in the first half of the twentieth century that continue to affect our mindset even now. The research includes human figures in the articles of women's magazines and cosmetic advertisements in the early $20^{th}$ century. The results are as follows: First, the change of perception in the human body: Instead of being a subject of preservation, the body has become a subject of sculpture with emphasis on health in the 1920's and on beauty in the 1930's. The recognition of the importance of the body has created intensive attention on physical training and an increased sense of hygiene. The body exposed to the public perceives itself through the eyes of others that alter one's own perception of oneself as well as become a target of evaluation. There is an additional emphasis on the exotic eroticism of a passive subordinate. Western culture became the standard for modernization along with the dissociation of traditional standards and values. Through the effect of education and western thinking, the awareness of women's rights and self-appreciation was developed. Second, ideal beauty can be summarized as follows: Unprocessed natural beauty was extolled as ideal in the 1920's, but the 1930's, it highlighted big eyes and an aquiline nose that are the characteristics of western women. Taking care of one's appearance was recognized as an important value for every social class. Cosmetics and skin care treatments promised soft and white skin. In contrast to western cosmetics, dark and shiny hair was highly favored. Exercising and traveling, differing seasonal and regional skin treatments were also widely accepted. In its initial stages, the research had originally assumed that the beginning of the twentieth century would be a time in which traditional concepts of beauty and new, westernized aesthetics coexisted. However, as the research progressed, it was clear that the idea of beauty had already adopted occidental ideals by that time. Thus, it seems necessary to continue the study on the shifting paradigms of beauty that must have occurred in the nineteenth and late twentieth century.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.34 no.5
• /
• pp.430-434
• /
• 2001

The algicidal activity of Shewanella (formerly Alteromonas) sp. SR-14 against diatom, Chaetoceros calcitrans was reported in our previous papers. In this study, the effect of Vibrio alginolyticus on the algicidal activity of Shewanelia sp, SR-14 was examined under the optimum algicidal conditions, i.e., temperature ($21\pm1^{\circ}C$), light intensity (4,000 lux), and light: dark cycle (12 hour: 12 hour). Shewanella sp. SR-14 grew well in the presence or the absence of V. alginolyticus in Conwy medium. Algal growth was only inhibited by Shewanella sp. SR-14. V. alginolyticus did not show the algicidal activity, Growth of C. calcitrans increased synergistically with growth of V. alginolyticus. When the initial inoculum of V. alginolyticus was only 1 log cycle higher than that of Shewanella sp. SR-14, the effect of V. alginolyticus on the algicidal activity of Shewanella sp. SR-14 was insignificant during incubation of mixed culture, i.e., two bacterial species and the alga. However, when V. alginolyticus dominated Shewanella sp. SR-14 by 3 log cycles of bacterial counts, it was found that the strain SR-14 could not inhibited growth of C. calcitrans up to 5 days of incubation.

• Journal of Mushroom
• /
• v.4 no.3
• /
• pp.111-115
• /
• 2006

Jinbudlsongyi mushroom (Agrocybe aegerita) was bred in Mushroom Research Institute, Gyonggi Province A.R.E.S in 2005. It was bred with mating between moookarytic strains isolated from Budlsongyi#1 and KME45202. The temperature of optimal mycelial growth was $24{\sim}26^{\circ}C$ on PDA and $18{\sim}20^{\circ}C$ in sawdust medium. To harvest fruiting body of Jiobudlsoogyi the period required for colonization after inoculation was 37days and the period of cultivation was 13days after scratching of inoculum. The major characteristics of the mushroom was shown a lot of primordia, light brown colored stipe and convex-shaped pileus. Compared with Budlsongyi#1, it was shown dark brown-colored pileus and straight stipe. The fruiting body yield was $115g{\pm}6/850cc$ bottle. It was demanded proper circulation at the time of primordia and put it around with cloth that is prevented to be bend the stipes.

• Korean Journal of Soil Science and Fertilizer
• /
• v.23 no.2
• /
• pp.152-159
• /
• 1990

Four active strains, producing siderophore and antagonizing to soil plant disease fungi, were isolated and identified from the wheat rhizosphere to reduce the injurious effects of continuous cropping and study on the biological control. The obtained results were summerized as follow. 1. Four strains of fluorescent Pseudomonas were isolated from the wheat rhizosphere and identified as Pseudomonas fluorescens Biotype A(Ps-1,2,5) and Biotype B(Ps-3) That strains inhibitied growth of R. solani, F.oxysperum and F. solani in vitro 2. Optical density of pigment was maximum at 410nm. 3. Siderophore production by identified strains was decreased with addition of $Fe^{+3}$, although not decreased with addition of $Fe^{+2}$ 4. Pigment of Ps-1, 2 and 3 strain inhibitied growth of R. slani, F. oxysperum and F. solani but pigment of Ps-5 strain did not inhibitie growth of R. solani 5. Effect of inoculation was in order of Ps-2, 1, 5 and 3 strain through the dark culture method, and effect of Ps-1 and Ps-2 was greater than that of treatment of captan 50ppm.

• The Korean Journal of Mycology
• /
• v.22 no.2
• /
• pp.190-195
• /
• 1994

Studies were conducted to determine the potential of sodium hypochlorite(SHC) on the control of bacterial yellow blotch in cultivated oyster mushroom, Pleurotus ostreatus. SHC at the concentration of 80 ppm was effective on the control of Pseudomonas agarici causing yellow blotch in oyster mushroom except number 916 isolate. In vitro the mycelial growth was slightly inhibited at the concentration higher than 100 ppm of sodium hypochlorite, but retardation of the mycelial growth was soon recovered. Spray of SHC solution at the concentration of 40-50 ppm per day significantly reduced the incidence of the yellow blotch without impairing the growth of oyster mushroom in field culture. However, the higher concentration of SHC(67 ppm) induced yellow brown or dark gray in color and deformed cap and elongated stripe in morphology of fruiting body. Results indicate that periodical spray of sodium hypochlorite seems to be the recommendable method for protection against bacterial yellow blotch disease in oyster mushroom without reducing food quality.

• The Korean Journal of Mycology
• /
• v.46 no.3
• /
• pp.271-280
• /
• 2018

The ectomycorrhizal basidiomycete Tricholoma is one of mushroom groups that cannot be cultivated artificially. To use this mushroom as applicable resource for food production, it is necessary to obtain information about their mycelial growth properties in various environmental conditions. This study investigated the mycelial growth of four domestic isolates of Tricholoma species (T. bakamatsutake, T. fulvocastaneum, T. matsutake, T. terreum) at different physical and chemical conditions. The optimal physical conditions for their mycelia growth were found to be a temperature range of $20{\sim}25^{\circ}C$ and a pH range of 4.0~7.0 in dark condition. The growth of T. matsutake was retarded at high temperature ($30^{\circ}C$). Tests to determine the chemical factors that affected mycelial growth showed that the four Tricholoma spp. grew 1% saline. T. matsutake grew in up to 2% saline. In the presence of various heavy metals (50 ppm) and pesticides (suppliers' recommended concentration), mycelial growth was inhibited the most by cadmium and emamectin benzoate, respectively. However, all the four Tricholoma spp. grew with $Cu^+$. The growth of T. matsutake was not inhibited by abamectin, acetamiprid, and thiacloprid. Extracellular enzyme activities of amylase and ${\beta}$-glucosidase were detected only in T. bakamatsutake and T. fulvocastaneum. The results of the present study allowed us to determine suitable or harmful environmental conditions for the mycelial cultivation of the Tricholoma spp.

• Korean journal of applied entomology
• /
• v.4
• /
• pp.19-24
• /
• 1965

In an attempt to find a satisfactory environmental factors which facilitate abundant conidial production of Piriculariaoryzae Cav. on tomato juice media, various environmental factors were studied for their effect on sporulation and mycelial growth of the fungus. Those factors were conditions of irradiation, color of light, age of culture and pH of the media. l) Continuous exposure to fluorescent light (Mitsubish FL-20-35 W) produced more conidia and much mycelial growth than did intermittent photoperiods and darkness. 2) Of 3 cellophane filters and direct exposure to fluorescent light used, conidia were produced best under the direct exposure to the light. Conidial production in color filter conditions sequently decreased with red, yellow and blue. Growth of mycelium was not significantly different within colors. 3) Periodic irradiation of 12-hour unit brought about zones on mycelial growth no matter what the color filter was used. 4) Older cultures responding to the light were more stimulated by light than were the younger one in the conidia production, but maximum production of conidia was 48 hours of age in this case. 5) Color of the mycelial mat and the aerial mycelium seemed to have a close relation to the production of conidia. The more darkness of the mycelial mat was produced the more conidia and the much aerial mycelium was produced the least conidia. The color of mycelium was more dark under the continuous irradiation than continuous darkness, while the periodic irradiation showed intermediate effect. 6) The concentration of hydrogen ion for growth and sporulation of the fungus was investigated the ranges between 5 and 9. The best pH for the fungus was also noted at 7. whereas the below of pH 4 was not occurred any mycelial growth and sporulation.