• Title/Summary/Keyword: damage level

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Nucleus-DNA Damage and Different Response of Plant Cells to Paraquat in Relation to Enzyme Activity of Superoxide Dismutase. (Superoxide dismutase의 활성차이에 따른 식물세포의 paraquat에 대한 반응과 핵 DNA 손상 검정)

  • 권순태;이명현;오세명;정도철;김길웅
    • Journal of Life Science
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    • v.14 no.4
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    • pp.614-619
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    • 2004
  • This study was undertaken to investigate the different responses of cultured plant cells to paraquat treatment and nucleus-DNA damage in relation to enzyme activity of superoxide dismutase (SOD). Furthermore, this study was also carried out to understand the antioxidative mechanism of plant cells to environmental stress. We selected two different species of plant cultured cells, Ipomoea batatas as high-SOD species and Lonicera japonica as low-SOD species. The total activity and specific activity of SOD in a chlorophyllous cell of I. batatas were 3,736 unit/gㆍfresh weight and 547 unit/mgㆍprotein, respectively, and those in L. japonica were 23 unit/gㆍfresh weight and 13 unit/mgㆍprotein, respectively SOD activity in chlorophyllous I. batatas cells reached its maximum level at 10 to 15 days after subculture, whereas that in L. japonica remained at a very low SOD level during the whole period of subculture. In comparison to L. japonica, I. batatas, a high-SOD species, showed high tolerance to paraquat 10 and 50 mg/l treatment in terms of cell viability and electrolyte leakage. Based on the result of comet assay, the nucleus-DNA damage of two species by paraquat 50 mg/l treatment was not significantly different. However, I. batatas cells repaired their damaged DNA more effectively than the cells of the low-SOD species, L. japonica.

Seismic failure analysis and safety assessment of an extremely long-span transmission tower-line system

  • Tian, Li;Pan, Haiyang;Ma, Ruisheng;Dong, Xu
    • Structural Engineering and Mechanics
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    • v.71 no.3
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    • pp.305-315
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    • 2019
  • Extremely long-span transmission tower-line system is an indispensable portion of an electricity transmission system, and its failures or collapse can impact on the entire electricity grid, affect the modern life, and cause great economic losses. It is therefore imperative to investigate the failure and safety of the transmission tower subjected to ground motions. In the present study, a detailed finite element (FE) model of a representative extremely long-span transmission tower-line system is established. A segmental damage indicator (SDI) is proposed to quantitatively assess the damage level of each segment of the transmission tower under earthquakes. Additionally, parametric studies are conducted to investigate the influence of different ground motions and incident angles on the ultimate capacity and weakest segment of the transmission tower. Finally, the collapse fragility curve in terms of the maximum SDI value and PGA is plotted for the exampled transmission tower. The results show that the proposed SDI can quantitatively assess the damage level of the segments, and thus determine the ultimate capacity and weakest segment of the transmission tower. Moreover, the different ground motions and incident angles have a significant influence on the SDI values of the transmission tower, and the collapse fragility curve is utilized to evaluate the collapse resistant capacity of the transmission tower subjected to ground motions.

Rapid retrofit of substandard short RC columns with buckled longitudinal bars using CFRP jacketing

  • Marina L. Moretti
    • Earthquakes and Structures
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    • v.24 no.2
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    • pp.97-109
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    • 2023
  • This experimental study investigates the effectiveness of applying carbon fiber reinforced polymer (CFRP) jackets for the retrofit of short reinforced concrete (RC) columns with inadequate transverse reinforcement and stirrup spacing to longitudinal rebar diameter equal to 12. RC columns scaled at 1/3, with round and square section, were subjected to axial compression up to failure. A damage scale is introduced for the assessment of the damage severity, which focusses on the extent of buckling of the longitudinal rebars. The damaged specimens were subsequently repaired with unidirectional CFRP jackets without any treatment of the buckled reinforcing bars and were finally re-tested to failure. Test results indicate that CFRP jackets may be effectively applied to rehabilitate RC columns (a) with inadequate transverse reinforcement constructed according to older practices so as to meet modern code requirements, and (b) with moderately buckled bars without the need of previously repairing the reinforcement bars, an application technique which may considerably facilitate the retrofit of earthquake damaged RC columns. Factors for the estimation of the reduced mechanical properties of the repaired specimens compared to the respective values for intact CFRP-jacketed specimens, in relation to the level of damage prior to retrofit, are proposed both for the compressive strength and the average modulus of elasticity. It was determined that the compressive strength of the retrofitted CFRP-jacketed columns is reduced by 90% to 65%, while the average modulus of elasticity is lower by 60% to 25% in respect to similar undamaged columns jacketed with the same layers of CFRP.

Development for the function of Wind wave Damage Estimation at the Western Coastal Zone based on Disaster Statistics (재해통계기반 서해 연안지역의 풍랑피해예측함수 개발)

  • Choo, Tai Ho;Kwak, Kil Sin;Ahn, Si Hyung;Yang, Da Un;Son, Jong Keun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.2
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    • pp.14-22
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    • 2017
  • The frequency and scale of natural disasters due to the abnormal climate phenomena caused by global warming have being increasing all over the world. Various natural disasters, such as typhoons, earthquakes, floods, heavy rain, drought, sweltering heat, wind waves, tsunamis and so on, can cause damage to human life. Especially, the damage caused by natural disasters such as the Earthquake of Japan, hurricane Katrina in the United States, typhoon Maemi and so on, have been enormous. At this stage, it is difficult to estimate the scale of damage due to (future) natural disasters and cope with them. However, if we could predict the scale of damage at the disaster response level, the damage could be reduced by responding to them promptly. In the present study, therefore, among the many types of natural disaster, we developed a function to estimate the damage due to wind waves caused by sea winds and waves. We collected the damage records from the Disaster Report ('91~'14) published by the Ministry of Public Safety and Security about wind waves and typhoons in the western coastal zone and, in order to reflect the inflation rate, we converted the amount of damage each year into the equivalent amount in 2014. Finally, the meteorological data, such as the wave height, wind speed, tide level, wave direction, wave period and so on, were collected from the KMA (Korea Meteorological Administration) and KHOA (Korea Hydrographic and Oceanographic Agency)'s web sites, for the periods when wind wave and typhoon damage occurred. After that, the function used to estimate the wind wave damage was developed by reflecting the regional characteristics for the 9 areas of the western coastal zone.

Permeability Characteristics related with Damage Process in Granites (화강암의 손상과정에 따른 투수계수 특성 연구)

  • 정교철;채병곤;김만일;서용석
    • The Journal of Engineering Geology
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    • v.11 no.3
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    • pp.315-325
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    • 2001
  • A series of laboratory tests was conducted to observe damage process by stress and to understand characteristics of permeability related with rock damage. Rock specimens which were composed of the Cretaceous medium grained granites were experienced of damage stress between 65% and 95% of the compressive strength. Rock deformation by damage process was identified with the elastic wave velocity test. Relationship between rock damage and permeability change was also analyzed by water injection test in the laboratory. According to the results of the tests, damage tends to be occurred from stress level of 80% of the compressive strength and it reduces elastic wave velocity. The damaged specimens with stress more than 80% of the compressive strength showed crack density more than 0.6 and persistent length with good connectivity of cracks. They also have higher permeability than that of specimens with crack density less than 0.6. Considered with the above results, the rock specimens used in this study were fully damaged from stress level of 80% of the compressive strength. Crack initiation and propagation by damage caused good connectivity of cracks through rock specimen. These damage process, therefore, brought high permeability coefficient through water flow conduit in the rock specimen.

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Adaptive Responses of Escherichia coli for Oxidative and Protein Damage Using Bioluminescence Reporters

  • Min, Ji-Ho;Gu, Man-Bock
    • Journal of Microbiology and Biotechnology
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    • v.14 no.3
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    • pp.466-469
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    • 2004
  • The recombinant bioluminescent Escherichia coli strains, DPD2511 and TV 1061 containing the katG and grpE promoters, respectively, from Vibrio fischeri fused to luxCDABE, were used to detect the adaptive and repair responses to oxidative damage caused by hydrogen peroxide $(H_2O_2)$, and protein damage due to phenol. The response ratio, represented as the bioluminescence induced in subsequent inductions of DPD2511 and TV1061 with the mother cells previously induced by each chemical, i.e., $H_2O_2$ and phenol during the previous induction stage, decreased suddenly compared with the ratio of the control culture of each strain, meaning there is a possible adaptive response to stress caused by chemicals. Protein damage due to phenol was completely repaired by the second culturing after the initial induction, as was oxidative damage caused by $H_2O_2$ which was also rapidly repaired, as detected by the recovery of bioluminescence level. This result suggests that E. coli promptly adapt and repair oxidative and protein damage by $H_2O_2$ and phenol completely.

Experimental study on identification of stiffness change in a concrete frame experiencing damage and retrofit

  • Zhou, X.T.;Ko, J.M.;Ni, Y.Q.
    • Structural Engineering and Mechanics
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    • v.25 no.1
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    • pp.39-52
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    • 2007
  • This paper describes an experimental study on structural health monitoring of a 1:3-scaled one-story concrete frame subjected to seismic damage and retrofit. The structure is tested on a shaking table by exerting successively enhanced earthquake excitations until severe damage, and then retrofitted using fiber-reinforced polymers (FRP). The modal properties of the tested structure at trifling, moderate, severe damage and strengthening stages are measured by subjecting it to a small-amplitude white-noise excitation after each earthquake attack. Making use of the measured global modal frequencies and a validated finite element model of the tested structure, a neural network method is developed to quantitatively identify the stiffness reduction due to damage and the stiffness enhancement due to strengthening. The identification results are compared with 'true' damage severities that are defined and determined based on visual inspection and local impact testing. It is shown that by the use of FRP retrofit, the stiffness of the severely damaged structure can be recovered to the level as in the trifling damage stage.

Improvement of MFL sensing-based damage detection and quantification for steel bar NDE

  • Kim, Ju-Won;Park, Minsu;Kim, Junkyeong;Park, Seunghee
    • Smart Structures and Systems
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    • v.22 no.2
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    • pp.239-247
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    • 2018
  • A magnetic flux leakage (MFL) method was applied to detect and quantify defects in a steel bar. A multi-channel MFL sensor head was fabricated using Hall sensors and magnetization yokes with permanent magnets. The MFL sensor head scanned a damaged specimen with five levels of defects to measure the magnetic flux density. A series of signal processing procedures, including an enveloping process based on the Hilbert transform, was performed to clarify the flux leakage signal. The objective damage detection of the enveloped signals was then analyzed by comparing them to a threshold value. To quantitatively analyze the MFL signal according to the damage level, five kinds of damage indices based on the relationship between the enveloped MFL signal and the threshold value were applied. Using the proposed damage indices and the general damage index for the MFL method, the detected MFL signals were quantified and analyzed relative to the magnitude of the damage increase.

Identification of Damage Characteristics Due to Cracking of Concrete Structures Using Acoustic Emission (음향방출 특성을 이용한 콘크리트 부재종류 및 하중상태에 따른 균열손상 연구)

  • 오병환;김응재;김광수;유성원
    • Journal of the Korea Concrete Institute
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    • v.11 no.4
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    • pp.107-116
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    • 1999
  • The damage in concrete structures generally starts with microcracking and thus it is important to find and explore these microcracks in concrete in order to ensure appropriate safety and serviceability. The purpose of the present study is to identify the damage characteristics of concrete structures due to cracking by employing the acoustic emission techniques. A comprehensive experimental study has been done. The cracking damage under tensile and flexural loadings have been identified and the bond damage between steel and concrete have been also characterized. It is seen that the amplitudes and energy level of Acoustic Emission(AE) events are found to be relatively small for bond cracking damages and large for tensile cracking damages. The characteristic equations of the AE events for various cracking damages have been proposed based on the present test data. The internal microcracks are progressively developed ahead of a visible actual crack and the present study clearly exhibits these damage mechanism for various types of cracking in concrete. The present study provides useful data which can be used to identify the various types of cracking damages in concrete structures. This will allow efficient maintenance of concrete structures through monitoring of internal cracking based on acoustic emission.

Establishing Policies towards Integrated Management of Soil Pollution and Damage (토양오염 및 훼손 통합관리를 위한 정책방향 설정)

  • Kim, Jong Sung;Park, Yoon-Sik;Lee, Gi-Ha;Hwang, Sang-Il;Yang, Jae E.
    • Journal of Soil and Groundwater Environment
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    • v.22 no.6
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    • pp.85-93
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    • 2017
  • In this study, the concepts of soil degradation, soil pollution and soil damage are defined, and the domestic and foreign administrative systems related to soil pollution and soil damage management are analyzed. In case of foreign countries, laws and regulations on the soil conservation and soil damage management were analyzed. In case of Korea, the present state of the legal system governing soil pollution and damage management was analyzed in each aspect. Through this study, suggestions for amendments of relevant laws were proposed by establishing policy direction for integrated management of soil pollution and soil damage. The results of this study will provide a basis for integrated management of soil pollution and damage, and it can be utilized in establishing integrated management strategy of long term soil conservation and sustaninable soil development at national level.