Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Nucleus-DNA Damage and Different Response of Plant Cells to Paraquat in Relation to Enzyme Activity of Superoxide Dismutase.

Superoxide dismutase의 활성차이에 따른 식물세포의 paraquat에 대한 반응과 핵 DNA 손상 검정

  • 권순태 (안동대학교 생명자원과학부) ;
  • 이명현 (안동대학교 생명자원과학) ;
  • 오세명 (안동대학교 생명자원과학) ;
  • 정도철 (상주대학교 자원식물학) ;
  • 김길웅 (경북대학교 농학과)
  • Published : 2004.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was undertaken to investigate the different responses of cultured plant cells to paraquat treatment and nucleus-DNA damage in relation to enzyme activity of superoxide dismutase (SOD). Furthermore, this study was also carried out to understand the antioxidative mechanism of plant cells to environmental stress. We selected two different species of plant cultured cells, Ipomoea batatas as high-SOD species and Lonicera japonica as low-SOD species. The total activity and specific activity of SOD in a chlorophyllous cell of I. batatas were 3,736 unit/gㆍfresh weight and 547 unit/mgㆍprotein, respectively, and those in L. japonica were 23 unit/gㆍfresh weight and 13 unit/mgㆍprotein, respectively SOD activity in chlorophyllous I. batatas cells reached its maximum level at 10 to 15 days after subculture, whereas that in L. japonica remained at a very low SOD level during the whole period of subculture. In comparison to L. japonica, I. batatas, a high-SOD species, showed high tolerance to paraquat 10 and 50 mg/l treatment in terms of cell viability and electrolyte leakage. Based on the result of comet assay, the nucleus-DNA damage of two species by paraquat 50 mg/l treatment was not significantly different. However, I. batatas cells repaired their damaged DNA more effectively than the cells of the low-SOD species, L. japonica.

Keywords

References

  1. Alscher, R. G. and J. L. Hess. 1993. Antioxidants in Higher Plants. pp. 1-174. CRC Press, Raton
  2. Bowler, C., W.Van-Comp, M.Van-Montagu and D. Inze. 1994. Superoxide dismutase in plants. CRC Critical Reviews in Plant Science 10, 199-218 https://doi.org/10.1080/713608062
  3. Bradford, M. M. 1976. A rapid sensitive method for the quantitation microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72, 265- 270 https://doi.org/10.1016/0003-2697(76)90527-3
  4. Fairbairn,D.W., P.L.Olivie and K.O′Neill. 1995. The comet assay : a comprehensive review. Mutation Research 339, 37-44 https://doi.org/10.1016/0165-1110(94)00013-3
  5. Furusawa, I., K. Tanaka, P. Thanutong, A. Mizuguchi, M. Yazaki and K. Asada. 1984. Paraquat resistant tobacco calluses with enhanced superoxide dismutase activity. Plant Cell Physiololgy 25, 1247-1254
  6. Hewitt, N., G. Kok and R. Fall. 1990. Hydrogen peroxide in plants exposed to ozone mediates air pollution damage to alkene emitters. Nature 344, 56-58 https://doi.org/10.1038/344056a0
  7. Kim, K. U., D. U. Kim and S. T. Kwon. 1986. Development of herbicide (paraquat) tolerant plant through tissue culture. Korean J. Weed Science 6, 191-200
  8. Kwak, S. S., S. K. Kim, S. H. Kim and J. R. Liu. 1995. , Korean J. Plant Research 23, 103-106
  9. Kwon, S. Y., Y. J. Jeong, H. S. Lee, J. S. Kim, K. Y. Cho, R. D. Allen and S. S. Kwak. 2002. Enhanced tolerances of transgenic tobacco plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against methyl viologene-mediated oxidative stress. Plant Cell Environment 25, 873-882 https://doi.org/10.1046/j.1365-3040.2002.00870.x
  10. McCord, J. M. and I. Fridovich. 1969. Superoxide dismutase. An enzymic function for erythrocuprein. J. Biol. Chemistry 244, 6049-6055
  11. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant 15, 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  12. Noroozi, M., W. J. Angerson and M. E. J. Lean. 1998. Effects of flavonoids and vitamin C on oxidative DNA damage to human lymphocytes. American J. Clinical Nutrients 64, 1210-1218
  13. Price, A. and A. Hendry. 1991. Iron-catalyzed oxygen radical formation and its possible contribution to drought damage in nine native grasses and three cereals. Plant Cell Environment 14, 477-484 https://doi.org/10.1111/j.1365-3040.1991.tb01517.x
  14. Rojas, E., M. C. Lopez and M. Valverde. 1999. Single cell gel electrophoresis assay; methodology and application. J. Chromatography B 772, 225-254 https://doi.org/10.1016/S0378-4347(98)00313-2
  15. Towill, L. E. and P. Mazur. 1975. Studies on reduction of 2,3,5-triphenylterazolium chloride as a viability assay for plant tissue culture. Canadian J. Botany 53, 1097-1102 https://doi.org/10.1139/b75-129
  16. Yun, B. W., G. H. Huh, H. S. Lee, S. Y. Kwon, J. K. Jo, J. S. Kim, K. Y. Cho and S. S. Kwak. 2000. Differential resistance to methyl-viologen in transgenic tobacco plants express sweetpotato peroxidase. J. Plant Physiology 156, 504-509 https://doi.org/10.1016/S0176-1617(00)80165-0