• Title/Summary/Keyword: damage/damage identification

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Isolation, Identification and Use of Bacterial Strain Ochrobactrum intermedium PDB-3 for Degradation of the Pesticide Chlorpyrifos

  • Diyorbek Kosimov;Lyudmila Zaynitdinova;Aziza Mavjudova;Muzaffar Muminov;Oybek Shukurov
    • Microbiology and Biotechnology Letters
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    • v.52 no.1
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    • pp.44-54
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    • 2024
  • One of the serious modern environmental problems is pollution caused by highly toxic pesticides. Only small amounts of applied pesticides reach their target, and the rest ends up in soil and water. Chlorpyrifos is a toxic, broad-spectrum organophosphate insecticide. In humans, chlorpyrifos inhibits acetylcholinesterase (AChE) in the peripheral and central nervous system, and particularly in children, small amounts of this pesticide cause neurotoxic damage. As the toxic effects of chlorpyrifos and its persistence in the environment require its removal from contaminated sites, it is essential to study the biological diversity of chlorpyrifos-degrading microorganisms. In this study, we sought to determine the chlorpyrifos-degrading ability of the bacterial strain Ochrobactrum intermedium PDB-3. This strain was isolated from soil contaminated with various pesticides and identified as PDB-3 based on morpho-cultural characteristics, MALDI-TOF MS, and 16S rRNA. Studies were conducted for 30 days in sterile soils containing initial concentrations of 50, 75, 100, and 125 mg/kg of chlorpyrifos. To determine the degradation of chlorpyrifos, a liquid culture of the strain was added to the soil at three optical densities: 0, and after 24 and 48 h (OD = 0.03, 0.2 and 0.32). Using GX-MS, we determined that chlorpyrifos was converted to 3,5,6-trichloro-2-pyridinol (TCP). We also found that with increasing optical density, rapid degradation of the initial concentration of chlorpyrifos occurred. Sterile soil without strain PDB-3 was used as a control sample.

Nonlinear intelligent control systems subjected to earthquakes by fuzzy tracking theory

  • Z.Y. Chen;Y.M. Meng;Ruei-Yuan Wang;Timothy Chen
    • Smart Structures and Systems
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    • v.33 no.4
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    • pp.291-300
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    • 2024
  • Uncertainty of the model, system delay and drive dynamics can be considered as normal uncertainties, and the main source of uncertainty in the seismic control system is related to the nature of the simulated seismic error. In this case, optimizing the management strategy for one particular seismic record will not yield the best results for another. In this article, we propose a framework for online management of active structural management systems with seismic uncertainty. For this purpose, the concept of reinforcement learning is used for online optimization of active crowd management software. The controller consists of a differential controller, an unplanned gain ratio, the gain of which is enhanced using an online reinforcement learning algorithm. In addition, the proposed controller includes a dynamic status forecaster to solve the delay problem. To evaluate the performance of the proposed controllers, thousands of ground motion data sets were processed and grouped according to their spectrum using fuzzy clustering techniques with spatial hazard estimation. Finally, the controller is implemented in a laboratory scale configuration and its operation is simulated on a vibration table using cluster location and some actual seismic data. The test results show that the proposed controller effectively withstands strong seismic interference with delay. The goals of this paper are towards access to adequate, safe and affordable housing and basic services, promotion of inclusive and sustainable urbanization and participation, implementation of sustainable and disaster-resilient buildings, sustainable human settlement planning and manage. Simulation results is believed to achieved in the near future by the ongoing development of AI and control theory.

Identification, Larval Host Plant Range, and Damage of the Fruit Sucking Moths to the Major Fruit in Cheonnam Province (주요(主要) 과수(果樹)에 대(對)한 흡수(吸收)나방류(類)의 종류(種類), 유충(幼蟲)의 기주범위(寄主範圍) 및 피해상(被害相)에 관(關)하여(전남(全南) 과수지대(果樹地帶) 중심(中心)))

  • Kim, Kyu-Chin;Lee, Tae-Sik
    • Korean journal of applied entomology
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    • v.24 no.4 s.65
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    • pp.183-190
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    • 1986
  • Identification of fruit sucking moths, their larval host plant, and degree of damages to fruits were investigated in $1982{\sim}'83$ at two orchards in Cheonnam province. In this study, 4 families 30 species were collected as fruit sucking moths and among them 10 species are newly recorded in Korea as fruit sucking moth: Catocala electa(B.), Spirama helicina(H.), Erebus ephesperis(H.), Parallelia stuposa(F.), Simplicia niphona(B.), Marumba gaschkewitschii(B. et G.), Agrius convolvuli(L.), Thyatira batis (L.), and Spilosoma niveum(M.). Larval host plants of fruit sucking moths in the vicinity of the fruit orchard include Cocculus trilobus D., Albizzia julibrissin D., Pterocarya rhoifolia S., Jaglans mandshurica M., Solanum melongena L., Brassia campestris S., Rubus idaeus C., Thalictrum aquilegifol L., and Rhus succedanea L. As the damage on different varieties of the main fruit trees, plum was orderly Santa Rosa>Beauty, Apple was Chook>Hong Og, Peach was Choseng Baeg Do>Hwang Do>Baeg Do, and Grape was Campbell Early>Neo Mascat>Golden Queen. Seasonal occurrence of main fruit sucking moth Spirama retorta(C.) showed peak 3 times in early-July, late-July, early-September. Oraesia emerginata(F.) showed peak 3 times in late-July, early-August, early- September and Lagoptera juno(D.) showed peak only in late-July and Adrias tyrannus amurensis(S.) showed peak only in mid-July. Fruit sucking moths were immigrated from p.m. 6 to a.m. 2, with peak in p.m. 10 to p.m. 12.

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DNA barcoding of Raptor carcass collected in the Paju city, Korea (파주시에서 수집한 폐사체 맹금류의 DNA 바코드 연구)

  • Jin, Seon-Deok;Paik, In-Hwan;Lee, Soo-Young;Han, Gap-Soo;Yu, Jae-Pyoung;Paek, Woon-Kee
    • Korean Journal of Environment and Ecology
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    • v.28 no.5
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    • pp.523-530
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    • 2014
  • One juvenile raptor which was not able to be identified due to its head damage was discovered on a roadside in Janggok-ri, Jori-eup, Paju on 28th June, 2011. The species was identified by DNA barcoding. After polymerase chain reaction (PCR) of the mitochondrial cytochrome c oxidase subunit I gene (COI), we obtained 695 bp sequences. We analyzed the obtained COI sequence with similar sequences from the BOLD systems and BLAST of the NCBI Genbank, and discovered that its sequence showed 100 % similarity values with the one of the five gray-faced buzzards which were previously researched. In addition, it was confirmed to be a female through sex determination using DNA. Such results are important information as it confirms the breeding of the gray-faced buzzards for the first time in 43 years since its breeding was last recorded in 1968, in Paju. Wildlife rescue center needs to work with adjacent consigned registration and preservation institutions when carcass of wild animals is collected or DNA samples are obtained for more accurate both species and sex identification through a systematic management system in the future. Furthermore, the obtained DNA sample of the gray-faced buzzard and COI gene, DNA barcode, could be used as reference standards for similar researches in the future.

Methodology for Selection and Sensitivity Index of Socio-economic Resources for Marine Oil Spill Incidents (해양 유류유출 오염으로 인한 사회·경제적 민감자원 선정 및 지수화 방안)

  • Roh, Young-Hee;Kim, Choong-Ki
    • Journal of Environmental Impact Assessment
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    • v.25 no.6
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    • pp.402-413
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    • 2016
  • Marine oil spill accidents are occurring continuously due to the marine transportation of the oil. While building a preventive system for oil spill is uttermost necessary, we also need to have a systematic response system to handle the oil spills that inevitably occur. So far, studies have focused on the environmentally sensitive resources affected by oil spills. However, there is a need to conduct research to evaluate the damage to the socially and economically sensitive resources that make up the life of local residents. This study represents the process of building an analytical framework for the assessment of socioeconomic resources affected by marine oil spills. While it is important to provide a scheme for identification and indexation of socially and economically sensitive resources that is compatible with Korea's situations, using existing data for identifying socio-economically sensitive resources might also be meaningful. However, to allow accurate analysis for better evaluation, we need to select more applicable data among the various indicators. In this research, we have reviewed many existing case studies of sensitive resources, studies of the variables that have been used for indexing sensitive resources, and various factors considered in SIA (Social Impact Assessment). Based on the findings, we classify socio-economically sensitive resources into marine products acquisition, population, land usage, administrative area, and cultural heritage and tourist region.

Identification and Molecular Characterization of Methionine Sulfoxide Reductase B Gene in Rice Blast Fungus, Magnaporthe oryzae (벼도열병균에서의 methionine sulfoxide reductase B 유전자의 분자적 특성)

  • Kim, Jeong-Hwan;Kim, Jin-Soo;Jeong, Mi-Yeon;Choi, Woo-Bong
    • Journal of Life Science
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    • v.19 no.3
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    • pp.343-348
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    • 2009
  • Magnaporthe oryzae, a major cause of rice blast, is one of the most destructive plant fungal pathogens. Secretion of reactive oxygen species (ROS) during the infection phase of plant pathogenic fungus plays a key role in the defense mechanism of a plant. ROS causes oxidative damage and functional modification to the proteins in a pathogenic fungus. Methionine, especially, is a major target of ROS, which oxidizes it to methionine sulfoxide. To survive from the attack of ROS, plant pathogenic fungus has antioxidative systems - one example would be methionine sulfoxide reductase B (MSRB), which reverses the oxidative alteration of methionine to methionine sulfoxide. In the present study, identification and molecular characterization of the MSRB gene in M. oryzae KJ201 were investigated. The MSRB gene was amplified by PCR from the M. oryzae KJ201 genomic DNA. The copy number of MSRB in the genome of M. oryzae KJ201 was identified by Southern blot analysis, which revealed that the gene exists as a single copy. To study the molecular function of an MSRB gene, the expression level of the MSRB gene was assayed with hydrogen peroxide treatment by Northern blot analysis and RT-PCR. The expression of the MSRB gene was increased by treatment of hydrogen peroxide, without significant correlation to hydrogen peroxide concentrations. These results indicate that the MSRB gene in M. oryzae KJ201 could contribute to protection against plant defense compounds such as ROS and offer a novel strategy for the control of rice blast.

Design of Automatic Classification System of Black Plastics Based on Support Vector Machine Using Raman Spectroscopy (라만분광법을 이용한 SVM 기반 흑색 플라스틱 자동 분류 시스템의 설계)

  • Bae, Jong-Soo;Oh, Sung-Kwun;Kim, Hyun-Ki
    • Journal of the Korean Institute of Intelligent Systems
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    • v.26 no.5
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    • pp.416-422
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    • 2016
  • Lots of plastics are widely used in a variety of industrial field. And the amount of plastic waste is massively produced. In the study of waste recycling, it is emerged as an important issue to prevent the waste of potentially useful resource materials as well as to reduce ecological damage. So, the recycling of plastic waste has been currently paid attention to from the view point of reuse. Existing automatic sorting system consist of near infrared ray (NIR) sensors to classify the types of plastics. But the classification of black plastics still remains a challenge. Black plastics which contains carbon black are not almost classified by NIR because of the characteristic of the light absorption of black plastics. This study is focused on handling how to identify black plastics instead of NIR. Raman spectroscopy is used to get qualitative as well as quantitative analysis of black plastics. In order to improve the performance of identification, Support Vector Machine(SVM) classifier and Principal Component Analysis(PCA) are exploited to more preferably classify some kinds of the black plastics, and to analyze the characteristic of each data.

A Study on the Factors Affecting the Intention of Chinese Users to Discriminate Against Fake News on Social Media - Focusing on attitude, social capital, and risk detection - (중국 이용자 소셜미디어 가짜뉴스 판별의도에 미치는 요인에 관한 연구 -태도, 사회자본, 위험감지를 중심으로-)

  • Tan, KeHong;Lee, Hwa Haeng
    • The Journal of the Korea Contents Association
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    • v.22 no.4
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    • pp.337-351
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    • 2022
  • With the full spread and rapid development of social media, the trend of decentralization of social media information propagation is becoming clearer day by day, and the segmentation of time by audiences using social media information is clearly progressing. Therefore, this study aims to study the influence relationship between social media attitudes toward fake news, social capital, risk perception, and discriminant intentions based on existing studies. Accordingly, the research model presented related research questions and organized a questionnaire to collect a total of 500 valid surveys. The SPSS 26.0 program and the AMOS 24.0 program were used to analyze the data. The research results are as follows. First, the more positive the user's attitude towards the fake news identification intention of social media, the more they want to use various methods or tools to identify the authenticity of online information. Second, the more positive the user's attitude towards social media fake news, the more aware of the potential threats social media fake news poses to their own physical, psychological, financial and so on. At the same time, by raising one's own awareness of the dangers, counterintelligence intentions against fake news on social media will also increase. Third, the richer the social capital the user has, the stronger the information literacy, and therefore the stronger the identification intention of social media fake news. Fourth, the higher the value of social capital Chinese users have, the greater the damage they have suffered from fake news, and the higher the risk awareness of fake news to protect their interests. Fifth, it means that Chinese users recognized information suspected of social media and took corresponding measures.

Conservation Treatment and Scientific Investigation of Daehye Bogakseonsaseo (Letters of Master Bogak) in the Goryeo Dynasty (고려시대 대혜보각선사서의 보존처리 및 과학적 조사)

  • Jang Yeonhee
    • Conservation Science in Museum
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    • v.29
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    • pp.47-64
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    • 2023
  • Daehye Bogakseonsaseo (Letters of Master Bogak) in the possession of the National Museum of Korea is a Goryeo-era book produced in 1387, which was acquired in 2005 in a state of severe general damage. Subsequently, its production techniques and materials were examined using bibliographical characteristics and scientific analysis, based on which an overall conservation treatment was carried out. Janghwang(mounting) in bibliographical characteristics is a five-hole-stitch binding (五針眼線裝本), a single-lined rectangular box (四周單邊), and no column lines. The conservation treatment was examined in two aspects: the basic form and the conservation treatment process. The book is composed of the outer cover, original cover, end paper, and inner paper of 0.04-0.07mm thick, which is significantly thinner than general book paper. The conservation treatment was applied after the entire book was disassembled, based on the assessment that both the cover and the inner sheets of paper were in a dire condition due to deterioration. The conservation treatment comprised of the process of disassembly, cleaning, reinforcement of defective parts, scanning, and binding, with a paulownia box made for its storage. At the time of disassembly, samples were made for the inner sheets and the book string in order to conduct fiber identification. The fibers were identified using Safranin and C-stains. Safranin staining identified a thick fiber wall, in addition to cross-marking, dislocation, and transparent membranes. C-stain staining identified the fiber as mulberry, given the dark red residue. The book strap has a flat, circular cross-section, and was identified as cotton fiber, since a lumen was observed in the center.

Characterization of Entomopathogenic Fungus from Trialeurodes vaporariorum and Evaluation as Insecticide (온실가루이 병원성 곰팡이의 특성 및 살충제 개발을 위한 평가)

  • Yoon, Hwi Gun;Shin, Tae Young;Yu, Mi Ra;Lee, Won Woo;Ko, Seung Hyun;Bae, Sung Min;Choi, Jae Bang;Woo, Soo Dong
    • Korean Journal of Microbiology
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    • v.49 no.1
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    • pp.64-70
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    • 2013
  • The greenhouse whitefly, Trialeurodes vaporariorum, is an economically important pest for greenhouse crops because they cause direct damage by feeding on plant nutrients and indirect damage as transmits many virus vectors. It has recently become a serious problem because of the continuous use of insecticide resulting in resistance among greenhouse whitefly population. To overcome these problems, in this study, the biological characteristics and virulence of an entomopathogenic fungus isolated from the cadaver of nymph greenhouse whitefly were investigated. Isolated fungus was identified as Isaria fumosorosea by morphological examinations and genetic identification using sequences of the ITS, ${\beta}$-tubulin, and EF1-${\alpha}$ regions. This fungus was named as I. fumosorosea SDTv and tested for the virulence against nymphs T. vaporariorum and the cold activity, the thermotolerance and the stability of UV-B irradiation on conidia. Mortality rate of greenhouse whitefly showed from 84 to 100% and the virulence increased with increasing conidial concentrations, $1{\times}10^5$ to $10^8$ conidia/ml. Conidia were stable at $35^{\circ}C$, 0.1 $J/cm^2$ of UV irradiation and germinated after 8 days at $4^{\circ}C$. Additionally, the activities of chitinases and proteases produced by I. fumosorosea SDTv were varied according to the medium. In conclusion, I. fumosorosea SDTv which showed high mortality rate against greenhouse whitefly will be used effectively in the integrated pest management programs against the greenhouse whitefly.