• Journal of Microbiology and Biotechnology
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• v.26 no.4
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• pp.775-783
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• 2016

The identification of novel reagents that exert a biological ultraviolet (UV)-protective effect in skin cells represents an important strategy for preventing UV-induced skin aging. To this end, we investigated the potential protective effects of Ettlia sp. YC001 extracts against UV-induced cellular damage in normal human dermal fibroblasts (NHDFs). We generated four different extracts from Ettlia sp. YC001, and found that they exhibit low cytotoxicity in NHDFs. The ethyl acetate extract of Ettlia sp. YC001 markedly decreased UVB-induced cytotoxicity. Additionally, the ethyl acetate extract significantly inhibited the production of hydrogen peroxide-induced reactive oxygen species. Moreover, it inhibited UVB-induced thymine dimers, as confirmed by luciferase assay and thymine dimer dot-blot assay. Thus, the study findings suggest Ettlia sp. YC001 extract as a novel photoprotective reagent on UVB-induced cell dysfunctions in NHDFs.

• Journal of Advanced Navigation Technology
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• v.20 no.1
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• pp.1-7
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• 2016

Since 2014, MOLIT (Ministry of Land, Infrastructure, and Transport) is carrying out a KASS project to develop and construct Korean SBAS. KASS can cause damage of human & properties if it has some problem during operation. Therefore, system safety assessment for KASS development is very important. Principal point of system safety assessment is the allocation of DAL(design assurance level) based on the hazard identification and classification. In this parer, the author conducts the allocation of DAL for KASS & its sub-systems based on the international standard(SAE ARP4761), which suggests a best practice of aviation system safety assessment. The result of this paper are the first step of system safety assessment, and can be used for further system safety assessment of KASS project.

• Proceedings of the Safety Management and Science Conference
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• 2009.04a
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• pp.35-43
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• 2009

The specific feature of trains as a means of transportation is that, on one side, at once they can carry big loads but, at the same time, if an accident occurs, it potentially leads to many human casualties or big material losses. Especially, train accidents caused by bad weather conditions result in many fatal losses of human lives and property. In Korea many railways run either in mountainous areas or along rivers thus making them especially susceptible to natural hazards. The types of damages inflicted by heavy rains resulting from rapidly changing meteorological conditions are diverse; and not only their scope is big but also they repeat regularly. Consequently, this study analyses the reasons why such effects of heavy rains on the railway conditions, damage to the railways caused by heavy rains or cases of stone fall as well as other types of accidents are not avoided. Study also, on the basis of laws related to movement in poor weather conditions and specifics of train braking, identifies systematic and technical problems and suggests and emphasizes new complex measures on their prevention.

• Korean journal of applied entomology
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• v.56 no.1
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• pp.87-92
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• 2017

The Samurai wasp, Trissolcus japonicus (Ashmead) (Hymenoptera: Platygasteridae) is recognized as a solitary parasitoid on eggs of the stink bug, Halyomorpha halys (Stål) (Hemiptera: Pentatomidae), an economic pest causing severe damage to fruits, in South Korea. The wasp appears to be a potential biological agent of the pest. In addition, T. itoi Ryu and T. nigripedius (Nakagawa) were found to parasitize eggs of Eurydema gebleri Kolenati and Dolycoris baccarum Linnaeus, respectively. Diagnostic characters and photographs are provided for identification, and host records of Korean Trissolcus species are also given.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2001.10a
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• pp.29-44
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• 2001

DNA damage by physical insult including UV and g-radiation might provoke genetic alterations in cells, which is followed by either acute cell death or tumorigenesis. The responsiveness to g-radiation depends on cellular context of target cells. To understand the mechanisms of checkpoint control, repair and cell death following genotoxic stimu]i, cDNA microarray can provide the gene expression profile. To make a profile of gene expression in irradiated Jurkat T cells, we hybridized the cDNA microarray using cDNA from g-irradiated Jurkat T cells. Jurkat T cells were exposed to 4Gy to 16Gy, and total RNA were extracted at 4 to 24 hrs after irradiation. The hybridization of the microarray to fluorescence-labeled cDNA from treated and untreated cells was analyzed by bioinformatic analysis to address relative changes in expression levels of the genes present in the array. Responses varied widely in different time points, suggesting acute stress response and chronic restoration or cell death. From these results we could select 384 genes related to radiation response in Tcells, and radiation response might be different in various types of cells. Using Radchip, we could separate "the exposed" from control PBMCs. We propose that Radchip might be useful to check the radiation research as well as radiation carcinogenesis.

• Parasites, Hosts and Diseases
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• v.51 no.5
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• pp.563-567
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• 2013

An ocular Toxocara canis infection is reported for the first time in Vietnam. A 34-year-old man residing in a village of Son La Province, North Vietnam, visited the National Eye Hospital (NEH) in August 2011. He felt a bulge-sticking pain in his left eye and loss of vision occurred over 3 months before visiting the hospital. The eye examination in the hospital showed damage of the left eye, red eye, retinal fibrosis, retinal detachment, inflammation of the eye tissues, retinal granulomas, and a parasitic cyst inside. A larva of Toxocara was collected with the cyst by a medical doctor by surgery. Comparison of 264 nucleotides of internal transcribed spacer 2 (ITS2) of ribosomal DNA was done between our Vietnamese Toxocara canis and other Toxocara geographical isolates, including Chinese T. canis, Japanese T. canis, Sri Lankan T. canis, and Iranian T. canis. The nucleotide homology was 97-99%, when our T. canis was compared with geographical isolates. Identification of a T. canis infection in the eye by a molecular method was performed for the first time in Vietnam.

• Childhood Kidney Diseases
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• v.24 no.1
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• pp.27-35
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• 2020

Purpose: Urinary tract infections (UTIs) are the most common and serious bacterial infections in children. Therefore, early diagnosis of vesicoureteral reflux (VUR) for treatment planning and the identification of noninvasive markers that can predict renal injury are important in patients with UTIs. We analyzed the clinical features of pediatric UTIs commonly encountered by general practitioners and reinterpreted the blood tests and imaging findings to identify the important clinical predictive markers of VUR in order to selectively perform VCUG. Methods: This retrospective study was performed among 183 children diagnosed with a UTI or acute pyelonephritis. Results: The most significant predictor of high grade and bilateral VUR identified using area under the curve analyses was hydronephrosis on kidney ultrasound images with renal cortical defects on dimercaptosuccinic acid (DMSA) kidney scan simultaneously, followed by hydronephrosis only on kidney ultrasound. Conclusion: The presence of hydronephrosis on kidney ultrasound images or cortical defects or asymmetric kidneys on the DMSA kidney scans can be predictive markers of VUR, reducing the need for VCUG. Our study can thus help minimize the exposure to radiation among patients through selective VCUG.

• Structural Engineering and Mechanics
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• v.54 no.2
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• pp.199-219
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• 2015

This paper presents the non-destructive evaluation of a high-speed railway bridge using train-induced strain responses. Based on the train-track-bridge interaction analysis, the strain responses of a high-speed railway bridge under moving trains with different operation status could be calculated. The train induced strain responses could be divided into two parts: the force vibration stage and the free vibration stage. The strain-displacement relationship is analysed and used for deriving critical displacements from theoretical stain measurements at a forced vibration stage. The derived displacements would be suitable for the condition assessment of the bridge through design specifications defined indexes and would show certain limits to the practical application. Thus, the damage identification of high-speed railways, such as the stiffness degradation location, needs to be done by comparing the measured strain response under moving trains in different states because the vehicle types of high-speed railway are relatively clear and definite. The monitored strain responses at the free vibration stage, after trains pass through the bridge, would be used for identifying the strain modes. The relationship between and the degradation degree and the strain mode shapes shows certain rules for the widely used simply supported beam bridges. The numerical simulation proves simple and effective for the proposed method to locate and quantify the stiffness degradation.

• Smart Structures and Systems
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• v.14 no.4
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• pp.679-697
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• 2014

The strain data acquired from structural health monitoring (SHM) systems play an important role in the state monitoring and damage identification of bridges. Due to the environmental complexity of civil structures, a better understanding of the actual strain data will help filling the gap between theoretical/laboratorial results and practical application. In the study, the multi-scale features of strain response are first revealed after abundant investigations on the actual data from two typical long-span bridges. Results show that, strain types at the three typical temporal scales of $10^5$, $10^2$ and $10^0$ sec are caused by temperature change, trains and heavy trucks, and have their respective cut-off frequency in the order of $10^{-2}$, $10^{-1}$ and $10^0$ Hz. Multi-resolution analysis and wavelet shrinkage are applied for separating and extracting these strain types. During the above process, two methods for determining thresholds are introduced. The excellent ability of wavelet transform on simultaneously time-frequency analysis leads to an effective information extraction. After extraction, the strain data will be compressed at an attractive ratio. This research may contribute to a further understanding of actual strain data of long-span bridges; also, the proposed extracting methodology is applicable on actual SHM systems.

• Plant Resources
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• v.5 no.3
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• pp.169-175
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• 2002

The soybean cyst nematode (Heterodera glycines Inchinoe; SCN) is a devastating pest of soybean and is responsible for significant losses in yield. The use of resistant cultivars is the effective method to reduce or eliminate SCN damage. The objective of this research is to identify AFLP markers linked to the SCN resistant genes. Bulked genomic DNA was made from resistant and susceptible genotypes to SCN and a total of 19 primer combinations were used. About 31 fragments were detected per primer combination. The banding patterns were readily distinguished in resistant and susceptible bulked genotypes. Polymorphic fragments were detected between resistant and susceptible bulked genotypes in the primer combination of CGT/GGC, CAG/GTG and CTC/GAG. In primer combinations of CGT/GGC and CAG/GTG, bulked resistant genotype produced a polymorphic bands. However, in primer of CTC/GAG, bulked susceptible genotype produced a polymorphic fragments. Three AFLP markers identified as a polymorphic fragments between bulked genomic DNA were mapped in 85 F2 population. Among them, only two markers, CGT/GGC and CTC/GAG, was linked and was mapped. Broad application of AFLP marker would be possible for improving resistant cultivars to SCN.