Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
권호 표지
DOI QR코드

DOI QR Code

Molecular Diagnosis of an Ocular Toxocariasis Patient in Vietnam

  • De, Nguyen Van (Department of Parasitology, Hanoi Medical University) ;
  • Trung, Nguyen Vu (National Hospital of Tropical Diseases) ;
  • Duyet, Le Van (National Hospital of Tropical Diseases) ;
  • Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine)
  • Received : 2013.07.06
  • Accepted : 2013.09.04
  • Published : 2013.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

An ocular Toxocara canis infection is reported for the first time in Vietnam. A 34-year-old man residing in a village of Son La Province, North Vietnam, visited the National Eye Hospital (NEH) in August 2011. He felt a bulge-sticking pain in his left eye and loss of vision occurred over 3 months before visiting the hospital. The eye examination in the hospital showed damage of the left eye, red eye, retinal fibrosis, retinal detachment, inflammation of the eye tissues, retinal granulomas, and a parasitic cyst inside. A larva of Toxocara was collected with the cyst by a medical doctor by surgery. Comparison of 264 nucleotides of internal transcribed spacer 2 (ITS2) of ribosomal DNA was done between our Vietnamese Toxocara canis and other Toxocara geographical isolates, including Chinese T. canis, Japanese T. canis, Sri Lankan T. canis, and Iranian T. canis. The nucleotide homology was 97-99%, when our T. canis was compared with geographical isolates. Identification of a T. canis infection in the eye by a molecular method was performed for the first time in Vietnam.

Keywords

References

  1. Holland C, Smith HV. Toxocara: The Enigmatic Parasite. Wallingford, UK and Cambridge, Massachusetts, USA. CABI Publishing. 2009.
  2. Choi D, Lim JH, Choi DC, Lee KS, Paik SW, Kim SH, Choi YH, Huh S. Transmission of T-oxocara canis via ingestion of raw cow liver: a cross-sectional study in healthy adults. Korean J Parasitol 2012; 50: 23-27. https://doi.org/10.3347/kjp.2012.50.1.23
  3. Finsterer J, Auer H. Neurotoxocarosis. Rev Inst Med Trop Sao Paulo. 2007; 49: 279-287. https://doi.org/10.1590/S0036-46652007000500002
  4. Noh Y, Hong ST, Yun JY, Park HK, Oh JH, Kim YE, Jeon BS. Meningitis by Toxocara canis after ingestion of raw Ostrich liver. J Korean Med Sci 2012; 27: 1105-1108. https://doi.org/10.3346/jkms.2012.27.9.1105
  5. Shields CL, Schoenberg E, Kocher K, Shukla SY, Kaliki S, Shields JA. Lesions simulating retinoblastoma (pseudoretinoblastoma) in 604 cases: results based on age at presentation. Ophthalmology 2013; 120: 311-316. https://doi.org/10.1016/j.ophtha.2012.07.067
  6. Woodhall D, Starr MC, Montgomery SP, Jones JL, Lum F, Read RW, Moorthy RS. Ocular toxocariasis: epidemiologic, anatomic, and therapeutic variations based on a survey of ophthalmic subspecialists. Ophthalmology 2012; 119: 1211-1217. https://doi.org/10.1016/j.ophtha.2011.12.013
  7. Kwon SI, Lee JP, Park SP, Lee EK, Huh S, Park IW. Ocular toxocariasis in Korea. Jpn J Ophthalmol 2011; 55: 143-147. https://doi.org/10.1007/s10384-010-0909-7
  8. Morais FB, Maciel AL, Arantes TE, Muccioli C, Allemann N. Ultrasonographic findings in ocular toxocariasis. Arq Bras Oftalmol 2012; 75: 43-47. https://doi.org/10.1590/S0004-27492012000100009
  9. John DT, Petri WA. Markell and Voge's Medical Parasitology. 9th ed. St. Louis, Missouri, USA. Saunders Elsevier. 2006.
  10. Zhou M, Chang Q, Gonzales JA, Chen Q, Zhang Y, Huang X, Xu G, Wang W, Jiang R. Clinical characteristics of ocular toxocariasis in Eastern China. Graefes Arch Clin Exp Ophthalmol 2012; 250: 1373-1378. https://doi.org/10.1007/s00417-012-1971-2
  11. Chen J, Zhou DH, Nisbet AJ, Xu MJ, Huang SY, Li MW, Wang CR, Zhu XQ. Advances in molecular identification, taxonomy, genetic variation and diagnosis of Toxocara spp. Infect Genet Evol 2012; 12: 1344-1348. https://doi.org/10.1016/j.meegid.2012.04.019
  12. Wang C. Toxocara canis internal transcribed spacer 1, partial sequence; 8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence. GenBank no. JN617989.1 (http://www.ncbi.nlm.nih.gov/nuccore/JN617989.1). 2011.
  13. Wickramasinghe S, Yatawara L, Rajapakse RP, Agatsuma T. Toxocara vitulorum (Ascaridida: Nematoda): mitochondrial gene content, arrangement and composition compared with other Toxocara species. Mol Biochem Parasitol 2009; 166: 89-92. https://doi.org/10.1016/j.molbiopara.2009.02.012
  14. Ishiwata K, Shinohara A, Yagi K, Horii Y, Tsuchiya K, Nawa Y. Toxocara canis genes for internal transcribed spacer 2, 5.8S ribosomal RNA, partial sequence, isolate: Tnj1. GenBank: AB110032.1 (http://www.ncbi.nlm.nih.gov/nuccore/AB110032.1). 2009.
  15. Jacobs DE, Gasser RB, Chilton NB. Toxocara canis rRNA gene, partial. 2003. GenBank no. Y09489.1 (http://www.ncbi.nlm.nih.gov/nuccore/Y09489.1). 2003.
  16. Khademvatan S, Rahim F, Tavalla M, Abdizadeh R, Hashemitabar M. PCR-based molecular characterization of Toxocara spp. using feces of stray cats: a study from Southwest Iran. PLoS One 2013; 8: e65293. https://doi.org/10.1371/journal.pone.0065293
  17. Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 2004; 101: 11030-11035. https://doi.org/10.1073/pnas.0404206101
  18. Stewart JM, Cubillan LD, Cunningham ET. Prevalence, clinical features and causes of vision loss among patients with ocular toxocariasis. Retina 2005; 25: 1005-1013. https://doi.org/10.1097/00006982-200512000-00009
  19. Arevalo JF, Espinoza JV, Arevalo FA. Ocular toxocariasis. J Pediatr Ophthalmol Strabismus 2013; 50: 76-86. https://doi.org/10.3928/01913913-20120821-01

Cited by

  1. Levels of Toxocara infections in dogs and cats from urban Vietnam together with associated risk factors for transmission vol.90, pp.4, 2016, https://doi.org/10.1017/s0022149x15000619
  2. Seroprevalence of fascioliasis, toxocariasis, strongyloidiasis and cysticercosis in blood samples diagnosed in Medic Medical Center Laboratory, Ho Chi Minh City, Vietnam in 2012 vol.9, pp.1, 2016, https://doi.org/10.1186/s13071-016-1780-2
  3. Toxocariasis: a silent threat with a progressive public health impact vol.7, pp.None, 2013, https://doi.org/10.1186/s40249-018-0437-0