• Proceedings of the Korean Society for Agricultural Machinery Conference
• /
• 1996.06c
• /
• pp.1045-1054
• /
• 1996

Tissue culture, or micropropagation , is being used for the vegetative multiplication of several hundred millions of superior plants annually for horticulture and forestry. It is often more expensive than other forms of propagation using cuttings or seeds, because it is labor intensive and more specialized . The aim of automation is to reduce the cost per plantlet by reducing labor input, and finally, to yield profit, as business activity . Labor usually account for 70-80% of th ein vitro and ex vitro cost. This paper aspects of tissue culture automization , such as technical and economical approaches in view of automization.

• Korean Journal of Plant Resources
• /
• v.15 no.1
• /
• pp.43-49
• /
• 2002

Effects of BA treatment and cut planting methods on shooting and seedling characteristics were investigated to increase seedling products by cuttings of lacquer tree. Dipping on 1,000mg/L BA solution was better than dripping with same solution at 5 weeks after cut planting. As shooting rate was increased suddenly, it was 85％ at 10 weeks after cut planting. Among 3 dripping treatments, interval was not effective on shooting rate. Dripping per 2 days with 100 ㎕ of 1,000 mg/L BA solution was most effective on seedling height and leaf number and than dipping was more effective than dripping per 5 and 7 days. Branch developed on near part from dripped scion. Shooting of horizontal planting was earlier about a week and rate was increased suddenly from 3 to 6 weeks, it was highest with 96％ than others at 10 weeks after planting. Seedling of horizontal cut planting was tallest about 40cm and branch developed lower than vertical and oblique cut planting. In case of horizontal cut planting, 2-3 shoots were formed on different part of scion and than those were could divided to seedling, so horizontal cut planting was most effective to propagation of seedling than any others.

• Journal of Bio-Environment Control
• /
• v.6 no.3
• /
• pp.225-234
• /
• 1997

This study was carried out to investigate the native distribution of wax myrtle (Myrica rubra) in Cheju Province and its propagation method. Wax myrtle is heavily distributed at 100 to 400m above sea level of Donhong-chun and Hyodon-chun which is bordered by Youngchon-dong of Seogwipo City and Haryeri of Namcheju county. BA, IBA, Kinetin, IAA, and GA3 were applied to promote rooting of cutting but were ineffective in promoting rooting. Treating cuttings with both AgNO,1 which removes rooting inhibitor, tannin and growth regulators such as BA, IBA and Kinetin were also ineffective in promoting rooting. Incubating seeds at 4$0^{\circ}C$ for 30 days resulted in 39％ germination. There was distinct difference in leaf shape between seedlings and mature trees.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2000.10a
• /
• pp.114-115
• /
• 2000

Aralia elata is found in mountain areas all over Korean peninsula. Aralia elata is the scientific name for Japanese angelica tree. The tree belongs to the family Araliaceae, commonly known as ginseng family. Bud sprouts from apical shoot tip of the plants are rich in flavor and thus mainly used for both folk medicine and vegetable. The stalks with apical buds are gathered in the early spring and planted in sandy soil or water in the greenhouse. The sprouting buds are then collected and sold as fresh vegetable. Although the plants have been used for food, they have been cultivated in a very small scale. In spring, local farmers just go around mountain areas to search the trees and gather the stalks as much as they get and sell them to the market. No conservation efforts have been made to stop the exploitation or to save the dwindling population. We tried to provide local farmers with the plants that may be used as an alternative to stalks from wild populations. This will bel! p conserve the wild populations. However, it is hard to propagate them either by conventional cuttings or by seed germination in a short period of time. Mass propagation using tissue culture systems have shown a great promise with several woody plants. Recently we developed a mass propagation technique via somatic embryogenesis system using mature and/or juvenile explants for Aralia elata. Several factors affecting somatic embryogenesis system including SE(somatic embryo) induction, embryogenic callus proliferation, SE germination, plant regeneration and transplanting to field frill be presented. And some problems arising for the somatic embryogenesis system will be also discussed.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2000.10b
• /
• pp.16-17
• /
• 2000

Aralia elata is found in mountain areas all over Korean peninsula. Aralia elata is the scientific name for Japanese angelica tree. The tree belongs to the family Araliaceae, commonly known as ginseng family. Bud sprouts from apical shoot tip of the plants are rich in flavor and thus mainly used for both folk medicine and vegetable. The stalks with apical buds are gathered in the early spring and planted in sandy soil or water in the greenhouse. The sprouting buds are then collected and sold as fresh vegetable. Although the plants have been used for food, they have been cultivated in a very small scale. In spring, local farmers just go around mountain areas to search the trees and gather the stalks as much as they get and sell them to the market. No conservation efforts have been made to stop the exploitation or to save the dwindling population. We tried to provide local farmers with the plants that may be used as an alternative to stalks from wild populations. This will hel! p conserve the wild populations. However, it is hard to propagate them either by conventional cuttings or by seed germination in a short period of time. Mass propagation using tissue culture systems have shown a great promise with several woody plants. Recently we developed a mass propagation technique via somatic embryogenesis system using mature and/ or juvenile explants for Aralia elata. Several factors affecting somatic embryogenesis system including SE(somatic embryo) induction, embryogenic callus proliferation, SE germination, plant regeneration and transplanting to field will be presented. And some problems arising for the somatic embryogenesis system will be also discussed.lso discussed.

• Journal of Forest and Environmental Science
• /
• v.37 no.4
• /
• pp.323-330
• /
• 2021

Zygotic embryo culture was performed to propagate evergreen oak, Quercus myrsinifolia, which has recalcitrant seeds and is difficult to propagate by cuttings. Zygotic embryos appeared in WPM medium after 14 days, and after 56 days, they developed into complete plants with cotyledons and roots. The medium suitable for zygotic embryo culture was 1/4 WPM medium, showing a shoot growth of 2.43 cm and root growth of 8.7 cm after 8 weeks of culture. As a result of investigating the effect of GA₃ on the growth of plants germinated from zygotic embryos through GA₃ treatment, the best growth was shown in 0.5 mg/l GA₃ treatment. The in vitro rooting and growth of IBA-treated zygotic embryo-derived plants were good in the 0.5 mg/l IBA treatment and rooting and shoot growth were not observed at higher concentrations. And the callus induction rate also increased as the concentration of IBA increased. Plants grown in vitro were transferred to a plastic pot containing artificial soil and acclimatized in a greenhouse for about 4 weeks, resulting in more than 90% survival. As a result of this study, the zygotic embryo culture method was confirmed to be effective for mass propagation of Q. myrsinifolia. The results of this study are expected to contribute significantly to the mass propagation of elite Q. myrsinifolia.

• Journal of Korean Society of Forest Science
• /
• v.40 no.1
• /
• pp.43-50
• /
• 1978

The elder berry was known to the rich in natural food colour resources and used in as for making wine, confectionary, perfumes, natural food colour, making elder berry juice, jelly, jam and medicinal properties or oils. In the present study, wish was to find the effect of various factors on the success of the vegetative propagation of elder berry by means cutting in the exposed field and green house was carried out and those obtained results can be summarized as follows. 1. Cuttings with dormant cutting stocs in the polyethylen house with heating and water mist spray resulting 100 percent survival. And temperature and the relative humidity in which fraim during the cutting season were around $20{\sim}25^{\circ}C$ and 70-90% respectively and in case as more significant other of the 1% Level. 2. With five varieties tested, resulting 93.8 percent survival, The F. value is not significant. 3. With four organs of cutting stock tested resulting 57.5 percent survival on the cuttings with two knodes of dormant cutting stock served as better cutting stock than others. The F. value is more significantly 1% Level. 4. Dormand bud served as possible cutting stock was found to be 17.66 survival percentage. 5. Both earlier and later stage of germinated Leaves with soft wood cutting stock poor cuttings, and the degree of development of 15th June cutting stock was optimum stage on the principal factor governing the success of cutting in the soft wood cutting showing 54% survival. The F. value is more 1% Level significant.

• Horticultural Science & Technology
• /
• v.18 no.6
• /
• pp.815-818
• /
• 2000

This study was conducted to determine the effect of rooting promoters and light intensity on rooting and root growth of rose cuttings. The cuttings, both 'Noblesse' and 'Red Velvet', were rooted up to 100% with Rootone treatment, while less than 100% with other growth promoter treatments. In 'Red Velvet', percentage of rooting increased with increasing IAA, NAA, and IBA concentrations. In 'Noblesse', however, the rate increased up to $500mg{\cdot}L^{-1}$ in NAA and IBA, and $1000mg{\cdot}L^{-1}$ in IAA, and then decreased above the concentrations. In 'Noblesse', root number, length, and weight increased by Rootone treatment. In 'Red Velvet', root number, and length increased by IBA treatment, while root weight was highest in Rootone treatment. In both cultivars, rooting was accelerated and the rate increased under high light intensity and root growth increased also. Between both cultivars, propagation and root growth of 'Red Velvet' decreased dramatically with decreased light intensity.

• Journal of Bio-Environment Control
• /
• v.31 no.3
• /
• pp.212-220
• /
• 2022

When it comes to single node leafy stem cuttings of rose (Rosa hybrida), environmental management such as air temperature, relative humidity, and light affect productivity. In order to investigate the effect of air temperature and relative humidity on the cutting success rate and rooted cuttings quality, a transparent airtight box was used to implement a closed system. We have also tried to find out the most effective photoperiod and the number of leaflets in closed system using artificial light (white LED, 104.0 µmol·m^-2·s^-1 photosynthetic photon flux density). The first experiment was conducted for a total of 6 weeks under 4 airtight period conditions. The number of roots and longest root length decreased as the airtight period increased. But there were no significant differences in the survival rate, shooting rate, and rooting rate according to airtight periods. In the second experiment the results indicated that survival and shooting rate were significantly affected by the photoperiod (0/24, 2/22, 4/20, 8/16, and 16/8 h), the number of leaflets (0, 2, and 4 leaflets) of the cuttings and their interaction. The survival rate was the highest in the 16-h day length and 4 leaflets. By considering survival rate and shooting rate with energy efficiency, the 8-h day length and 2 or 4 leaflets were judged to be the most effective.

• Journal of Bio-Environment Control
• /
• v.23 no.4
• /
• pp.288-292
• /
• 2014

This study was performed to investigate the possibility of water cutting by vegetative propagation method from July to August of Clematis. To find out proper cutting length and rooting growth regulator for water cuttings production, cutting lengths of C. 'Honora' made 1 node and 2 nodes length cutting, which were soaked in NAA $0.1mg{\cdot}L^{-1}$. Rooting rate of cuttings treated by NAA $0.1mg{\cdot}L^{-1}$ was 100% irrespective of root lengths, but that of 1 node and 2 nodes untreated by NAA $0.1mg{\cdot}L^{-1}$ were very low about 30% and 19% respectively. Number of roots and roots length were also highest in NAA $0.1mg{\cdot}L^{-1}$, effects of NAA $0.1mg{\cdot}L^{-1}$ treatment were greater at 1 node cutting. Based on the results, rooting rate for I node cutting of C. 'Pairu' according to NAA (0, 0.1, 0.5, $1.0mg{\cdot}L^{-1}$) concentrations was investigated. Days to rooting were 70-74 days (about 10 weeks), which was not significantly different among the NAA concentrations. Rooting rate increased by NAA concentration. it was about 90% usually and was improved with more than NAA $0.5mg{\cdot}L^{-1}$.