Journal of Forest and Environmental Science

Institute of Forest Science, kangwon National University (강원대학교 산림과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Propagation by In Vitro Zygotic Embryos Cultures of the Quercus myrsinifolia

  • Choi, Eun ji (Plant Conservation Division, Korea National Arboretum of the Korea Forest Service) ;
  • Yong, Seong Hyeon (Department of Forest Environmet Resource & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Seol, Yu Won (Department of Forest Environmet Resource & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Dong Jin (Department of Seed and Seedling Management, National Forest Seed and Cultivar Center) ;
  • Park, Kwan Been (Department of Forest Environmet Resource & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Do Hyun (Department of Forest Environmet Resource & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jin, Eon Ju (Forest Biomaterials Research Center, National Institute of Forest Science) ;
  • Choi, Myung Suk (Department of Forest Environmet Resource & Institute of Agriculture and Life Science, Gyeongsang National University)
  • Received : 2021.08.02
  • Accepted : 2021.10.27
  • Published : 2021.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Zygotic embryo culture was performed to propagate evergreen oak, Quercus myrsinifolia, which has recalcitrant seeds and is difficult to propagate by cuttings. Zygotic embryos appeared in WPM medium after 14 days, and after 56 days, they developed into complete plants with cotyledons and roots. The medium suitable for zygotic embryo culture was 1/4 WPM medium, showing a shoot growth of 2.43 cm and root growth of 8.7 cm after 8 weeks of culture. As a result of investigating the effect of GA3 on the growth of plants germinated from zygotic embryos through GA3 treatment, the best growth was shown in 0.5 mg/l GA3 treatment. The in vitro rooting and growth of IBA-treated zygotic embryo-derived plants were good in the 0.5 mg/l IBA treatment and rooting and shoot growth were not observed at higher concentrations. And the callus induction rate also increased as the concentration of IBA increased. Plants grown in vitro were transferred to a plastic pot containing artificial soil and acclimatized in a greenhouse for about 4 weeks, resulting in more than 90% survival. As a result of this study, the zygotic embryo culture method was confirmed to be effective for mass propagation of Q. myrsinifolia. The results of this study are expected to contribute significantly to the mass propagation of elite Q. myrsinifolia.

Keywords

Acknowledgement

This study was carried out with the support of "Forest BioResource Collection, Conservation and Characteristic Evaluation" of the National Forest Seed and Variety Center.

References

  1. Bonner FT. 1996. Responses to Drying of Recalcitrant Seeds of Quercus nigra L. Ann Bot 78: 181-187. https://doi.org/10.1006/anbo.1996.0111
  2. Chalupa V. 1984. In vitro propagation of oak (Quereus robur L.) and linden (Tilia cordata Mill.). Biol Plant 26: 374-377. https://doi.org/10.1007/BF02898577
  3. Cornea-Cipcigan M, Pamfil D, Sisea CR, Margaoan R. 2020. Gibberellic Acid Can Improve Seed Germination and Ornamental Quality of Selected Cyclamen Species Grown Under Short and Long Days. Agronomy 10: 516. https://doi.org/10.3390/agronomy10040516
  4. Dohling S, Kumaria S, Tandon P. 2008. Optimization of nutrient requirements for asymbiotic seed germination of Dendrobium longicornu and D. formosum Roxb. Proc Indian Natl Sci Acad 74: 167-171.
  5. Frick EM, Strader LC. 2018. Roles for IBA-derived auxin in plant development. J Exp Bot 69: 169-177. https://doi.org/10.1093/jxb/erx298
  6. Hanyan X, Hongmei Du, Danfeng H. 2006. Effects of GA_3 on the Seed Germination of Cyclamen Persicum. Seed 4: 62.
  7. Koene FM, Amano E, Ribas LLF. 2019. Asymbiotic seed germination and in vitro seedling development of Acianthera prolifera (Orchidaceae). S Afr J Bot 121: 83-91. https://doi.org/10.1016/j.sajb.2018.07.019
  8. Korea Forest Service. 2018. Forest Resources. https://www.forest.go.kr/kfsweb/. Accessed 3 Jan 2018.
  9. Kumar R, Ram M, Gaur GS. 2010. Effect of GA3 and ethrel on growth and flowering of African marigold cv. Pusa Narangi Gainda. Indian J Hortic 67: 362-366.
  10. Lewis S, Phillips O, Baker T, Malhi Y, Lloyd J. 2006. Tropical forests and atmospheric carbon dioxide: current knowledge and potential future scenarios. In: Avoiding Dangerous Climate Change (Schellnhuber HJ, Cramer W, Nakicenovic N, Wigley T, Yohe G, eds). Cambridge University Press, Cambridge, pp 147-153.
  11. Lloyd G, McCown B. 1981. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Proc Int Plant Propag Soc 30: 421-427.
  12. Metz B, Davison O, Bosch P, Dave R, Meyer L. 2007. Climate Change 2007: Mitigation of Climate Change. Cambridge University Press, Cambridge.
  13. Murashige T, Skoog F. 1962. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiol Plant 15: 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  14. Ostrolucka MG, Bezo M. 1994. Utilization of meristem cultures in propagation of oak (Quercus sp.). Genet Pol 35: 161-169.
  15. Park BB, Byun JK, Kim WS, Sung JH. 2010. Growth and Tissue Nutrient Responses of Fraxinus rhynchophylla, Fraxinus mandshurica, Pinus koraiensis, and Abies holophylla Seedlings Fertilized with Nitrogen, Phosphorus, and Potassium at a Nursery Culture. J Korean For Soc 99: 85-95.
  16. Park DJ, Yong SH, Choi MS. 2019. Establishment of Selection Method for Cold-Tolerant Individuals through Evaluating Tolerance of Evergreen Quercus spp. against Cold Stress. J For Environ Sci 35: 232-239.
  17. Park JC. 2007. Studies on the Growth of Seedlings and Transplanting in Quercus myrsinaefolia Blume and Q. glauca Thunb. MS thesis. Jinju National University, Jinju, Korea. (in Korean)
  18. Sanchez MC, San-Jose MC, Ballester A, Vieitez AM. 1996. Requirements for in vitro rooting of Quercus robur and Q. rubra shoots derived from mature trees. Tree Physiol 16: 673-680. https://doi.org/10.1093/treephys/16.8.673
  19. Son SG, Kim HJ, Kang YJ, Oh CJ, Kim CS, Byun KO. 2011. Establishment of Breeding Population for Quercus glauca and Climatic Factors. Korean J Agric For Meteorol 13: 109-114. https://doi.org/10.5532/KJAFM.2011.13.3.109
  20. Song KS, Choi KS, Sung HI, Jeon KS, An KJ, Kim JJ. 2015. Characteristics of Seedling Quality of Daphniphyllum macropodum 2-year-old Container Seedlings by Shading Level. J Korean For Soc 104: 390-396. https://doi.org/10.14578/jkfs.2015.104.3.390
  21. Stanys V, Mazeikiene I, Staniene G, Siksnianas T. 2007. Effect of phytohormones and stratification on morphogenesis of Paeonia lactiflora Pall. isolated embryos. Biologija 18: 27-30.
  22. Urbanova T, Leubner-Metzger G. 2016. Gibberellins and seed germination. Annu Plant Rev 49: 253-284. https://doi.org/10.1002/9781119210436.ch9
  23. Van Waes JM, Debergh PC. 1986. In vitro germination of some Western European orchids. Physiol Plant 67: 253-261. https://doi.org/10.1111/j.1399-3054.1986.tb02452.x
  24. Vidal N, Arellano G, San-Jose MC, Vieitez AM, Ballester A. 2003. Developmental stages during the rooting of in-vitro-cultured Quercus robur shoots from material of juvenile and mature origin. Tree Physiol 23: 1247-1254. https://doi.org/10.1093/treephys/23.18.1247
  25. Vudala SM, Ribas LLF. 2017. Seed storage and asymbiotic germination of Hadrolaelia grandis (Orchidaceae). S Afr J Bot 108: 1-7. https://doi.org/10.1016/j.sajb.2016.09.008
  26. Wen F, Zhang Z, Bai T, Xu Q, Pan Y. 2010. Proteomics reveals the effects of gibberellic acid (GA3) on salt-stressed rice (Oryza sativa L.) shoots. Plant Sci 178: 170-175. https://doi.org/10.1016/j.plantsci.2009.11.006
  27. Zeiger E, Grivet C, Assmann SM, Deitzer GF, Hannegan MW. 1985. Stomatal Limitation to Carbon Gain in Paphiopedilum sp. (Orchidaceae) and its Reversal by Blue Light. Plant Physiol 77: 456-460. https://doi.org/10.1104/pp.77.2.456