• Animal cells and systems
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• 제12권1호
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• pp.1-9
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• 2008

In the developing limb, chondrogenesis is an important prerequisite for the formation of cartilage whose template is required for bone formation. Chondrogenesis is a tightly regulated multi-step process, including mesenchymal cell recruitment/migration, prechondrogenic condensation of the mesenchymal cells, commitment to the chondrogenic lineage, and differentiation into chondrocytes. This process is controlled exquisitely by cellular interactions with the surrounding matrix and regulating factors that initiate or suppress cellular signaling pathways and transcription of specific genes in a temporal-spatial manner. Understanding the cellular and molecular mechanisms of chondrogenesis is important not only in the context of establishing basic principle of developmental biology but also in providing research direction toward preventive and/or regenerative medicine. Here, I will overview the current understanding of cellular and molecular mechanisms contributing to prechondrogenic condensation processes, the crucial steps for chondrogenesis, focusing on cell-cell and cell-matrix interactions.

• International Journal of Stem Cells
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• 제15권1호
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• pp.60-69
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• 2022

Organoids show great potential in clinical translational research owing to their intriguing properties to represent a near physiological model for native tissues. However, the dependency of organoid generation on the use of poorly defined matrices has hampered their clinical application. Current organoid culture systems mostly reply on biochemical signals provided by medium compositions and cell-cell interactions to control growth. Recent studies have highlighted the importance of the extracellular matrix (ECM) composition, cell-ECM interactions, and mechanical signals for organoid expansion and differentiation. Thus, several hydrogel systems prepared using natural or synthetic-based materials have been designed to recreate the stem cell niche in vitro, providing biochemical, biophysical, and mechanical signals. In this review, we discuss how recapitulating multiple aspects of the tissue-specific environment through designing and applying matrices could contribute to accelerating the translation of organoid technology from the laboratory to therapeutic and pharmaceutical applications.

• 한국세라믹학회지
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• 제31권4호
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• pp.375-380
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• 1994

Matrices retaining electrolyte in phosphoric acid fuel cell were prepared with SiC to SiC whisker mixing ratios of 1:0.5, 1:1, 1:1.5, 1:2, 1:3 by tape casting method. When viscosity of the slurry was 5.9 poise and the SiC to SiC whisker mixing ratios were 1:1, 1:1.5, 1:2, the ranges of porosity, acid absorbency and bubble pressure were 80~90%, 2.5~6 and 700~2200 mmH2O, respectively. Those ranges are acceptable for a practical electrolyte-retaining matrix. With increasing the mixing ratio of SiC whisker to SiC, the porosity and the vol.% of large pores in the main pore size distribution which is between 1 and 10 ${\mu}{\textrm}{m}$, increased rapidly. Impedance spectroscopy was measured to know characteristics of matrix inside and contact region of matrix to catalyst layer. When the SiC to SiC whisker mixing ratio was 1:2, hydrogen ions were transported in the matrix most effectively because of high ionic conductivity and low activation energy due to high acid absorbency in spite of high interfacial resistance. The cell current density of the cell made using the matrix was 220 mA/$\textrm{cm}^2$ at 0.7 V.

• 대한치과보철학회지
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• 제44권1호
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• pp.73-84
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• 2006

The importance of soft tissue response to implant abutments has become one of the major issues in current implant dentistry. To date, numerous studies have emphasized on maintaining connective tissue barriers in quantity, as well as in quality fir the long term success of dental implants. The cells mainly consisting the soft tissue around dental implants are fibroblasts and epithelial cells. The mechanism of the fibroblasts adhesions to certain substrata can be explained by the 'focal adhesion' theory. On the other hand, epithelial cells adhere tn the substratum via hemidesmosomes. The typical integrin-mediated adhesions of cells to certain matrix are called 'cell-matrix adhsions'. The focal adhesion complex of fibroblasts, in relation to the cell-matrix adhsions, consists of the extracellular matrix(ECM) such as fibronectin, the transmembrane proteins such as integrins, the intracellular cytoplasmic proteins such as vinculin, talin, and more, and the cytoskeletal structures such as filamentous actin and microtubules. The mechanosensory function of integrins and focal adhesion complexes are considered to play a major role in the cells adhesion, migration, proliferation, differentiation, division, and even apoptosis. The '3-D matrix adhesions' defined by Cukierman et al. makes a promising future for the verification of the actual process of the cell-matrix adhesions in vivo and can be applied to the field of implant dentistry in relation to obtaining strong soft tissue attachment to the implant abutments.

• 전자공학회논문지C
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• 제36C권8호
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• pp.10-21
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• 1999

본 논문에서는 12비트의 해상도와 65MHz의 변환속도를 가지면서 단일 3.3V의 공급전압으로 동작하는 전류 셀 매트릭스 구조의 CMOS D/A 변환기를 제안하였다. 설계된 CMOS D/A 변환기는 우수한 단조증가성과 빠른 정착시간을 가지는 전류 셀 매트릭스 구조의 장점을 이용하면서 기존의 D/A 변환기의 전류셀 간의 문턱전압의 부정합과 접지선의 전압 강하에 의한 오차를 감소시키기 위해 트리 구조 바이어스 회로, 대칭적 접지선 연결, 캐스코드 전류 스위치를 사용하여 구현되었다. 설계된 전류 셀 매트릭스 12비트 D/A 변환기를 $0.6{\mu}m$ CMOS n-well 공정을 이용하여 제작하였다. 제작된 DAC칩을 +3.3V 단일 공급전원을 이용하여 측정한 결과, 정착시간이 20nsec로써 50MHz의 변환속도와 35.6mW의 전력소모를 나타내었다. 또한 측정된 SNR, DNL은 각각 55 dB, ${\pm}0.5LSB$,${\pm}2LSB$를 나타내었다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회A
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• pp.1455-1457
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• 2008

Cell adhesion is a coordinated process involving initial binding of integrin receptors to extracellular matrix (ECM), recruitment of adhesion proteins, and focal adhesion assembly. The formation of mechanically stable focal adhesion assembly of cells within surrounding ECM is a key parameter to direct numerous cellular functions including cell migration, differentiation, and apotosis. With current cell adhesion assays, it is difficult to understand contributions of each coordinated event on evolution of cell adhesion strengthening since cells spontaneously spread upon their adhesion to the substrate, thus remodeling their cytoskeletal structure. In this presentation, novel approaches for analysis of cell adhesion strengthening process based on the combination of mechanical device, micro-patterned substrates, and molecular biological techniques will be discussed.

• JSTS:Journal of Semiconductor Technology and Science
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• 제12권3호
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• pp.270-277
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• 2012

This paper presents a new DAC design strategy to achieve a wideband dynamic linearity by increasing the bandwidth of the output impedance. In order to reduce the dominant parasitic capacitance of the conventional matrix structure, all the cells associated with a unit current source and its control are stacked in a single column very closely (stacked unit cell structure). To further reduce the parasitic capacitance, the size of the unit current source is considerably reduced at the sacrifice of matching yield. The degraded matching of the current sources is compensated for by a self-calibration. A prototype 6-bit 3.3-GS/s current-steering full binary DAC was fabricated in a 1P9M 90 nm CMOS process. The DAC shows an SFDR of 36.4 dB at 3.3 GS/s Nyquist input signal. The active area of the DAC occupies only $0.0546mm^2$ (0.21 mm ${\times}$ 0.26 mm).

• IMMUNE NETWORK
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• 제20권6호
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• pp.46.1-46.13
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• 2020

Neutrophils are innate immune cells that constitute the first line of defense against invading pathogens. Due to this characteristic, they are exposed to diverse immunological environments wherein sources for nutrients are often limited. Recent advances in the field of immunometabolism revealed that neutrophils utilize diverse metabolic pathways in response to immunological challenges. In particular, neutrophils adopt specific metabolic pathways for modulating their effector functions in contrast to other immune cells, which undergo metabolic reprogramming to ensure differentiation into distinct cell subtypes. Therefore, neutrophils utilize different metabolic pathways not only to fulfill their energy requirements, but also to support specialized effector functions, such as neutrophil extracellular trap formation, ROS generation, chemotaxis, and degranulation. In this review, we discuss the basic metabolic pathways used by neutrophils and how these metabolic alterations play a critical role in their effector functions.

• 대한전자공학회논문지SD
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• 제43권12호
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• pp.15-22
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• 2006

본 논문에서는 UWB(Ultra Wide Band)통신시스템을 위한 1.8V 8-bit 500MSPS의 D/A 변환기를 제안한다. 전체적인 D/A 변환기의 구조는 높은 선형성과 낮은 글리치 특성을 갖는 상위 6-MSB(Most Significant Bit) 전류원 매트릭스(Current Cell Matrix)와 하위 2-LSB(Least Significant Bit) 전류원 매트릭스로 구성된 2단 매트릭스 구조로 설계하였다. 또한 동일한 지연시간을 갖는 Thermometer Decoder와 고속 동작에서 전력을 최소화하기 위한 저 전력 스위칭 디코더(Current Switching Decoder Cell)를 제안함으로서 D/A 변환기의 고속 동작에서 성능을 향상시켰다 설계된 DAC는 1.8V의 공급전압을 가지는 TSMC $0.18{\mu}m$ 1-poly 6-metal N-well CMOS 공정으로 제작되었으며, 제작된 D/A 변환기의 측정결과, 매우 우수한 동적성능을 확인하였다. 500MHz 샘플링 클럭 주파수와 50MHz의 출력신호에서 SFDR은 약 49dB, INL과 DNL은 각각 0.9LSB, 0.3LSB 이하로 나타났으며, 이 때의 전력소비는 약 20mW로 기존의 8-bit D/A변환기에 비해 매우 낮음을 확인 할 수 있었다 D/A 변환기의 유효 칩 면적은 $0.63mm^2(900um{\times}700um)$이다.

• Bulletin of the Korean Chemical Society
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• 제32권2호
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• pp.605-608
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• 2011

Hybrid composite membranes were prepared by coating poly(ethylene oxide) and $SiO_2$ particles onto the porous polypropylene nonwoven matrix. Gel polymer electrolytes prepared by soaking the hybrid composite membranes in an organic electrolyte solution exhibited ionic conductivities higher than $1.1{\times}10^{-3}Scm^{-1}$ at room temperature. Dyesensitized solar cell (DSSC) employing the hybrid composite membrane with PEO and 10 wt % $SiO_2$ exhibited an open circuit voltage of 0.77 V and a short circuit current of 10.78 $mAcm^{-2}$ at an incident light intensity of 100 $mWcm^{-2}$, yielding a conversion efficiency of 5.2%. DSSC employing the hybrid composite membrane showed more stable photovoltaic performance than that of the DSSC assembled with liquid electrolyte.