• Biotechnology and Bioprocess Engineering:BBE
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• 제8권5호
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• pp.269-278
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• 2003

Gibberellic acid (GA$_3$) is a commercially important plant growth hormone, which is gaining much more attention all over the world due to its effective use in agriculture and brewing industry. Industrially it is produced by submerged fermentation technique using Ascomycetous fungus Gibberella fujikuroi. Solid state and immobilized cell fermentation techniques had also been developed as an alternative to obtain higher yield of GA$_3$. This review summarizes the problems of GA$_3$ fermentation such as production of co-secondary metabolites along with GA$_3$, substrate inhibition and degradation of GA$_3$ to biologically inert compound gibberellenic acid, which limits the yield of GA$_3$ in the fermentation medium. These problems can be overcome by various bioprocessing strategies e.g. two - stage and fed batch cultivation processes. Further research on bioreactor operation strategies such as continuous and / or extractive fermentation with or without cell recycle / retention system need to be investigated for improvement in yield and productivity. Down stream processing for GA$_3$ isolation is also a challenge and procedures available for the same have been critically evaluated.

• Plant Resources
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• 제6권3호
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• pp.205-210
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• 2003

Ginseng(Panax ginseng C.A. Meyer) is a perennial herbaceous plant which grows very slowly. It takes about 3 to 4 years from seeding to collecting the ripe seeds and the ginseng propagation is very difficult. and so, it is very difficult to breed ginseng plant. Ginseng tissue culture was started from at 1960, and ginseng commercial product by in vitro callus culture was saled, however upto now, regenerants were not planted to soil normally. Recently, plant genetic engineering to produce transgenic plants by introducing useful genes has been advanced greatly. In a present paper, transformation of ginseng plants was achieved by co-cultivation with Agrobacterium harboring the binary vector coding Proteinase-II gene, which confer resistant or tolerant to insect pests, The binary vector for transformation was constructed with disarmed Ti-plasmid and with double 35S promoter. The NPT II gene and introduced genes of the transgenic ginseng plants were successfully identified by the PCR. Especially the transgenic ginseng plants were regenerated using new techniques such as repetitive single somatic embryogenesis.

• Journal of Microbiology and Biotechnology
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• 제26권2호
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• pp.364-372
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• 2016

Sand tubules, made up of sand grains cemented by microbe-induced calcium carbonate precipitation, have been found in China's Ningxia Province. Sand tubules grow like a tree's roots about 40-60 cm below the surface. The properties of sand tubules and their bacterial community were examined. X-Ray diffraction analysis revealed that the sand tubules were associated with crystalline calcite. Scanning electron microscopy showed that the crystalline solid had a lamellar structure and lacked the presence of cells, suggesting that no bacteria acted as nucleation sites, nor that the crystalline solid was formed by the aggregation of bacteria. Denaturing gradient gel electrophoresis analysis showed 11 of the 12 detectable bands were uncultured bacteria by BLAST analysis in the GenBank database, and the rest were closely related to Paenibacillus sp. (100% identity). By cultivation techniques, the only strain isolated from the sand tubule was suggested to be related to Paenibacillus sp.; no archaea were found. Furthermore, Paenibacillus sp. was demonstrated to induce calcium carbonate precipitation in vitro.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2000년도 하계학술대회 논문집 D
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• pp.2772-2774
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• 2000

According to the development of marine transportation business and the modernization and over sizing of vessel transportation equipment. shipping equipment used in the terminal are getting more atomized and speedier. thus the efficiency of shipping and discharging depends upon the degree of practice of terminal operating manpower. It means that cultivation of experts in this field is essentially needed and it is becoming a serious problem to guarantee the training programs to provide high-class. high-skilled manpower. The best result can be expected when we use the real equipment for training purposes. but it will cause many difficulties such as budget problem. To overcome this situation. we developed the Virtual Container Crane Simulator(VCCS System) which enables the operator to learn the operation of the equipment as well as safety problems within a short time. VCCS system begins with establishing the direction of operation by setting the appropriate motions for each step. and then it instructs the basic operation. It enhances the driver's ability to cope with accidents during operation and completes and completes the mastery of operating techniques with The repetition of practice.

• KSBB Journal
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• 제5권2호
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• pp.101-106
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• 1990

Aureobasidium pullulans에 의한 플루란 생산이 다양한 pH,탄소원, 질소원 조건하에서 수행되었다. 발효 배양액과 배출기체 조성은 GC,LC,GPC를 이용하여 분석되었다. 초기 pH 6.0이고 sucrose 50g/l를 사용했을 때 27g/l 의 플루란이 생산되었다. pH가 3이하로 내려가면 균체량은 증가하지만 플루란 생산은 거의 정지되었다. 질소원으로$(NH_4)_2SO_4$만 사용한 경우의 발효에서는 pH가 3이하로 감소하였기 때문에 플루란 생산량이 감소하였기 때문에 플루란 생산량이 적었으며 탄소원으로 포도당을 사용했을 때도 비슷한 현상이 관찰되었다. sucrose같은 2당류를 기질로 사용했을 때 2당은 포도당과 3당류로 분해되고 포도당은 세포내에서 플루란으로 중합된 후 세포 밖으로 배출되는 것으로 추론되었다.

• 산업경영시스템학회지
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• 제30권4호
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• pp.155-163
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• 2007

Future war needs a lot of changes for military organization. Specially, concept of the "Speed" is raised importantly after Iraq war of U.S. Armed Forces. So, He is a lot of studying supply chain for logistical support speed improvement. Korea Marine Corps has made an efforts to improve supply chain. However it is real that is putting various techniques by prescription rather than fundamental change. These simplicity prescriptions are not only systematic but also insufficient for Marine Corps' survival. In addition, Korea marine corps in actuality is depending on many members in marine and navy in feed chain. Thus it is not establishing even tactical, decision-making plan of operation for support ability cultivation. Therefore, in this study, with Sense and Response concept that is United States Marine Corps' support strategy, presented theoretical background and basis frame that serve that Korea Marine Corps's supply chain changes to perception reaction formation.

• Plant Resources
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• 제3권3호
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• pp.194-199
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• 2000

The result of this experiment which are conducted, to improve the cultivation technology of Alisma plantago, to increase its quantity and to contribute for stable production with Yongiun local group by examining the optimal planting density and transplanting period of double cropping of Alisma plantago in the southern region. The characters of plant height, leaf width and length tend to be reduced as the seeding period is later by the order of the 10th, 20th and 30th of July. The period required for flowering is reduced as the transplanting period is later and dense planting is applied. Plant height, the number of leaves and yield of dry root have much quantity at the dense planting density of 20$\times$ 15cm as they are transplanted later in the 30th of August or the 10th of September, but they are rather less in sparse planting density of 20$\times$25cm or 20$\times$35cm.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1998년도 21세기 한반도 농업전망과 대책(한국작물학회.한국육종학회 공동주관 심포지움 회보)
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• pp.189-209
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• 1998

Food resources have been supplied more and more by crop land expansion, technological Improvement for higher crop yield, establishment of irrigation system, and input of fertilizers, chemicals and others, to sustain a world population increase currently about 6 billion. Food demand will be significantly increased in the 21st century due to population increases of 90 million per year and more consumption of meat with per capital income increase. But food production increase will be limited by difficulty of crop/irrigation land expansion and small or decreasing effectiveness of fertilizer use. Development of new techniques for higher yield per ha is only one way to meet future food demand increase. Optimistic prospect for food demand/supply balance was reported by FAO until 2010, and IFPRI until 2020. However, Worldwatch Institute warned world food supply will be less than expected demand by 500 million tons of cereal grains in 2030. It is necessary to establish a national plan to meet expected worldwide shortage of food resources in 21 century. What planning should be under taken to meet the upcoming century of food shortage in Korea whose food self-sufficiency rate is only $30{\%}$. It is recommended that (1) keep paddy field area as much as 1,100 thousand ha, (2) expansion of barley and wheat cultivation on all paddy area in winter season, (3) continue development of new technology to get international superiority of food resources produced in Korea, (4) expand nationwide the importance of food security under the current financial crisis encountered In Korea, and for food security in the future unified Korean peninsula.

• 한국식품과학회지
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• 제49권4호
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• pp.415-420
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• 2017

Soybean sprouts are the third-most consumed vegetable in Korea. Several studies on cultivation techniques, including use of medicinal plants extracts, have been performed to enhance the quality and yield of soybean sprouts. The objective of the present study was to investigate the effect of lacquer, a medicinal plant extract, on the yield and nutritional value of soybean sprouts. Linolenic acid content was significantly (p<0.05) increased in the sprouts produced by soaking seeds in lacquer extract diluted with equal volume of water. Lacquer extract significantly increased the flavonoid and phenolic contents (p<0.05). The content of total free amino acids, including ${\gamma}$-aminobutyric acid, was higher in lacquer extract-treated sprouts than in the untreated control. Results of this study suggest that lacquer extract could be used for enhancing the yield and nutritional values of soybean sprouts.

• 원예과학기술지
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• 제33권1호
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• pp.106-113
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• 2015

Effects of mineral salts (N, P, K, $Ca^{2+}$, $Mg^{2+}$, and $Fe^{3+}$) on the shoot growth and metabolite production of tea tree were studied using in vitro culture techniques. Among mineral s alts, ${H_2PO_4}^-$ was the most important for enhanced growth rate of tea tree, while $Mg^{2+}$ and $Ca^{2+}$ did not affect plant growth. Removal of ${NH_4}^+$ and $NO_3$ from the culture medium enhanced shoot multiplication compared to other treatments. Metabolite production was variable depending on mineral types and concentration. Removal of $Ca^{2+}$ decreased the production of caffeine; however, other treatments did not influence its production. $Ca^{2+}$, ${NH_4}^+$ and $Fe^{3+}$ were important factors for catechin production in tea tree. These results can be used as the basis for development of technical soil controls suitable for tea tree cultivation in the future.