DOI QR코드

DOI QR Code

Influence of Mineral Salts on Shoot Growth and Metabolite Biosynthesis in Tea Tree (Camellia sinensis L.)

  • Kim, Yong Duck (Institute of Hadong Green Tea) ;
  • Yun, Jae Gill (Department of Horticulture, Gyeongnam National University of Science and Technology) ;
  • Seo, Yeong Rong (Division of Environmental Forest Secience, Gyeongsang National University) ;
  • Karigar, Chandrakant S. (Department of Biochemistry, Tumkur University) ;
  • Choi, Myung Suk (Division of Environmental Forest Secience, Gyeongsang National University)
  • Received : 2014.04.02
  • Accepted : 2014.10.23
  • Published : 2015.02.28

Abstract

Effects of mineral salts (N, P, K, $Ca^{2+}$, $Mg^{2+}$, and $Fe^{3+}$) on the shoot growth and metabolite production of tea tree were studied using in vitro culture techniques. Among mineral s alts, ${H_2PO_4}^-$ was the most important for enhanced growth rate of tea tree, while $Mg^{2+}$ and $Ca^{2+}$ did not affect plant growth. Removal of ${NH_4}^+$ and $NO_3$ from the culture medium enhanced shoot multiplication compared to other treatments. Metabolite production was variable depending on mineral types and concentration. Removal of $Ca^{2+}$ decreased the production of caffeine; however, other treatments did not influence its production. $Ca^{2+}$, ${NH_4}^+$ and $Fe^{3+}$ were important factors for catechin production in tea tree. These results can be used as the basis for development of technical soil controls suitable for tea tree cultivation in the future.

Keywords

References

  1. Albert, R. and H. Kinzel. 1973. Distinction between physiotypes of halophytic colonizers of sea-area (Eastreach). Plant Physiol. 70:138-157.
  2. Bondarev, N.O., O. Reshetnyak, and A. Nosov. 2003. Effects of nutrient medium composition on development of Stevia rebaudiana shoots cultivated in the roller bioreactor and their production of steviol glycosides. Plant Sci. 165:845-850. https://doi.org/10.1016/S0168-9452(03)00283-8
  3. Chae, H.J., S.N. Yu, and D.G. Kim, 2006. Soil microflora and microfauna in 29 years of N-P-K fertilizer omission plot. Res. Plant Dis. 12:108-114. https://doi.org/10.5423/RPD.2006.12.2.108
  4. Chatuavedi, I. 2006. Effects of phosphorus levels alone or in combination with phosphate-solubilizing bacteria and farmyard manure on growth, yield and nutrient up-take of wheat (Triticum aestivum). J. Agric. Social Sci. 2:96-100.
  5. Eden, T. 1958. The development of tea culture, p. 1-4. In: T. Eden (ed.). Tea, Longman, London.
  6. Fageria, N.K., V.C. Baligar, C.A. Jones. 1997. Common bean and cowpea, p. 441-490. In: N.K. Fageria, V.C. Baligar, and C.A. Jone (eds.). Growth and mineral nutrition of field crops. Marcel Dekker, New York.
  7. Hagendoorn, M., A.M. Wagner, G. Segers, L. Van Der Plas, A. Oostdam, and H.S. Van Walraven. 1994. Cytoplasmic acidification and secondary metabolite production in different plant cell suspensions (a comparative study). Plant Physiol. 106:723-730.
  8. Harrington, H.M., S.L. Berry, and R.R. Henke. 1981. Amino acid transport into cultured Tobacco cells: II. Effect of calcium. Plant Physiol. 67:379-384. https://doi.org/10.1104/pp.67.2.379
  9. Kato, M., Y. Gyoten, K. Sakai-Kato, and T. Toyo'oka. 2003. Rapid analysis of amino acids in Japanese green tea by microchip electrophoresis using plastic microchip and fluorescence detection. J. Chromatogr. A 1013:183-189. https://doi.org/10.1016/S0021-9673(03)01037-9
  10. Klimaszewska, K. and W.A. Keller. 1985. High frequency plant regeneration from thin cell layer explants of Brassica napus. Plant Cell Tiss. Org. Cult. 4:183-197. https://doi.org/10.1007/BF00040193
  11. Kim, Y.D., J.Y. Min, M.J. Jeong, H.J. Song, J.G. Hwang, C.S. Karigar, G.W. Cheong, and M.S. Choi. 2010. Rapid selection of catechins-rich tea-trees (C. sinensis L.) by a colorimetric method. J. Wood Sci. 56:411-417. https://doi.org/10.1007/s10086-010-1112-7
  12. Marschner, H. 1995. Mineral nutrition of higher plants. 2nd ed. Academic Press, London.
  13. Mengel, K. and E.A. Kirkby. 1987. Principles of plant nutrition, p. 347-384. In: K. Mengel and E.A. Kirby (eds.). Interational Potash Institute, Bern, Switzerland.
  14. Mittal, K.N., B.N. Lal, and C.K. Dwivedi. 1978. Effect of fertilizer on the productivity and quality of wheat. J. Agric. Res. 27:19-3.
  15. Mohamed, A. A., I. H. El Sokkary, and T. C. Tucker. 1987. Growth and chlorophyll, mineral, and total amino acid composition of tomato and wheat plants in relation to nitrogen and iron nutrition II. Chlorophyll content and total amino acid composition. J. Plant Nutr. 10:713-731. https://doi.org/10.1080/01904168709363602
  16. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15:473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  17. Orians, C.M., S. Lower, R.S. Fritz, and B.M. Roche. 2003. The effects of plant genetic variation and soil nutrients on secondary chemistry and growth in a shrubby willow, Salix sericea: Patterns and constraints on the evolution of resistance traits. Biochem. Syst. Ecol. 31:233-247. https://doi.org/10.1016/S0305-1978(02)00144-8
  18. Park, T.G. and S.D. Song. 1995. Growth responses and regulations of nitrogen fixation activities of three leguminous plant treated with nitrogen and phosphorus. J. Environ. Sci. 9:127-136.
  19. Poothong, S. and B.M. Reed. 2014. Modeling the effects of mineral nutrition for improving growth and development of micropropagated red raspberries. Sci. Hortic. 165:132-141. https://doi.org/10.1016/j.scienta.2013.10.040
  20. Possingham, J.V. 1957. The effect of mineral nutrition on the content of free amino acids and amides in Tomato plants II. A study of the effect of molybdenum nutrition. Australian J. Biological Sci. 10:40-49.
  21. Sawwan, J., R.A. Shibi, I. Swaidat, and M. Tahat. 2000. Phosphorus regulates osmotic potential and growth of African violet under in vitro induced water deficit. J. Plant. Nutr. 23:759-771.
  22. Seetharam, Y.N., L.N. Rajanna, G. Jyothishwaran, B. Aravind, G. Sharanabasappa, and P.B. Mallikharjun. 2007. In vitro multiple shoot regeneration from nodal explants of Vernonia amygdalina-an important medicinal plant. Afr. Crop Sci. Conf. Proc. 8:747-752.
  23. Shabala, S. and Y. Hariadi. 2005. Effects of magnesium availability on the activity of plasma membrane ion trans-porters and light-induced responses from broad bean leaf mesophyll. Planta 221:56-65. https://doi.org/10.1007/s00425-004-1425-0
  24. Shear, C.B. and M. Faust. 1980. Nutritional ranges in deciduous tree fruits and nuts. Hort. Rev. 2:142-163.
  25. Shaul, O. 2002. Magnesium transport and function in plants: The tip of the iceberg. Biometals 15:309-323.
  26. Sheldon, V.L., W.M.G. Blue, and W.M.A. Albrecht. 1951. Biosynthesis of amino acids according to soil fertility. Plant Soil 3:361-365. https://doi.org/10.1007/BF01394033
  27. Shibli, R.A., M.M. Mohammad, A. Abu-Ein, and M. Shatnawi. 2000. Growth and micronutrient acquisition of some apple varieties in response to gradual in vitro induced salinity. J. Plant Nutr. 23:1209-1215. https://doi.org/10.1080/01904160009382094
  28. Sudha, G. and G.A. Ravishankar. 2002. Involvement and interaction of various signaling compounds on the plant metabolic events during defense response, resistance to stress factors, formation of secondary metabolites and their molecular aspects. Plant Cell Tiss. Org. Cult. 71:181-212. https://doi.org/10.1023/A:1020336626361
  29. Zhao, J., K. Fujita, and K. Sakai. 2005. Oxidative stress in plant cell culture: A role in production of h-thujaplicin by Cupresssus lusitanica suspension culture. Biotechnol. Bioeng. 90:621-631. https://doi.org/10.1002/bit.20465
  30. Zhong, J.J. and S.J. Wang. 1998. Effects of nitrogen source on the production of ginseng saponin and polysaccharide by cell cultures of Panax quinquefolium. Process Biochem. 33:671-675. https://doi.org/10.1016/S0032-9592(98)00030-2