• Applied Biological Chemistry
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• v.39 no.3
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• pp.177-182
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• 1996

Effects of 14 carbohydrates supplied as carbon sources on cell growth and sporulation of, and the production of insecticidal crystal proteins by Bacillus thuringiensis strains were investigated in liquid cultures. Strains grew well in media containing any one of the 14 carbohydrates supplied, reaching maximum cell densities of $10^7{\sim}10^8\;cells/ml$ in 16.7 to 22 hours after inoculation depending on the strain. Spores first appeared in 16.7 to 24.7 hours after inoculation, and 80% sporulation was reached in 28 to 51.3 hours after inoculation depending on the strain. No change in pH of media was observed after cell multiplication. The production of total protein was highest when supplied with sucrose and was lowest with starch. More insecticidal crystal proteins were produced when supplied with glucose, lactose, maltose, or sucrose. The amount of insecticidal crystal proteins produced by the strains was proportional to that of the total protein. The relative amount of individual insecticidal crystal protein species produced by B.t. kurstaki and B.t. israelensis was not influenced by the carbohydrates supplied.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.25 no.12
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• pp.996-999
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• 2012

From UV irradiation, we achieved homeotropic liquid crystal alignment on blended photo-polymer layer which is composed of polyvinyl-cinnamate (PVCi) and homeotropic polyimide (PI). From vertical alignment (VA) mode, we measured threshold voltages by various PVCi doping concentration. Also, the rise time and fall time of VA cells were measured to verify the best doping concentration. Transmittance curves showed about 70% value between 380 nm and 780 nm wavelength which mean visible region.

• Interaction and multiscale mechanics
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• v.1 no.4
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• pp.467-476
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• 2008

In this work, a nano-size rocksalt crystal (magnesium oxide) is considered as a continuous collection of unit cells, while each unit cell consists of discrete atoms; and modeled by a multiscale concurrent atomic/continuum field theory. The response of the crystal to an electromagnetic (EM) wave is studied. Finite element analysis is performed by solving the governing equations of the multiscale theory. Due to the applied EM field, the inhomogeneous motions of discrete atoms in the polarizable crystal give rise to the change of microstructure and the polarization wave. The relation between the natural frequency of this system and the driving frequency of the applied EM field is found and discussed.

• Korean Journal of Optics and Photonics
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• v.14 no.5
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• pp.498-503
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• 2003

We analyze the electro-optic polarization transmission characteristics of liquid crystal cells in the Poincare sphere representation. We determine fundamental conditions on maximizing of brightness and contrast ratio of liquid crystal display devices for polychromatic light by use of retardation films. For optimizing two colors, at least two properly designed retardation films are needed, and for three wavelengths, either it can be approximated to the two-color case or three retardation films are needed.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1996.11a
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• pp.180-182
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• 1996

In this study, we have investigated the characteristics of the response time and the viewing angle on twisted nematic (TN)-liquid crystal display (LCD) and amorphous (a) TN-LCD without rubbing. To measure the transmission-voltage, response time, and viewing angle characteristics, we prepared three kinds of LC cells and then studied the surface liquid crystal alignment effect. We studied that the response time on a-TN-LCD is fast compared to the TN-LCD. We suggest that the weak anchoring strength is attributed to the fast response time on a-TN-LCD. Also, we obtained the uniform viewing angle characteristics on a-TN-LCD. From these results, we consider that the liquid crystal alignment on LCD device are very important factor for electro-optical characteristics.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.15 no.6
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• pp.252-259
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• 2005

Single crystal $CuGaSe_2$ layers were grown on thoroughly etched semi-insulating CaAs(100) substrate at $450^{\circ}C$ with hot wall epitaxy (HWE) system by evaporating $CuGaSe_2$ source at $610^{\circ}C$. The crystalline structure of the single crystal thin films was investigated by the photoluminescence (PL) and double crystal X-ray diffraction (DCXD). The carrier density and mobility of single crystal $CuGaSe_2$ thin films measured with Hall effect by Van der Pauw method are $4.87{\times}10^{17}cm^{-3}$ and $129cm^2/V{\cdot}s$ at 293 K, respectively. The temperature dependence of the energy band gap of the $CuGaSe_2$ obtained from the absorption spectra was well described by the Varshni's relation, $E_g(T)=1.7998eV-(8.7489{\times}10^{-4}eV/K)T^2/(T+335K)$. The voltage, current density of maxiumun power, fill factor, and conversion, efficiency of $n-CdS/p-CuGaSe_2$, heterojunction solar cells under $80mW/cm^2$ illumination were found to be 0.41 V, $21.8mA/cm^2$, 0.75 and 11.17%, respectively.

• Journal of Microbiology and Biotechnology
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• v.14 no.4
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• pp.772-776
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• 2004

Physiological and molecular characteristics of Bacillus thuringiensis SR6 and SR8 were investigated, and phase contrast and electron microscopies revealed that a large rhomboidal crystal protein was present in the sporulating cells. SDS-PAGE and Western blot analyses showed that B. thuringiensis SR8 produced 70 kDa protein much more than other proteins, and that the 70 kDa protein could bind to the antibody of B. thuringiensis subsp. tenebrionis-crystal toxin protein, indicating that the crystal 70 kDa protein has an immunological homology with B. thuringiensis subsp. tenebrionis-crystal toxin protein. The DNA fragment of B. thuringiensis subsp. tenebrionis-toxin gene was detected in B. thuringiensis SR6 and SR8 by using PCR amplification analysis. Furthermore, the insect bioassay showed the insecticidal activity against Colorado potato beetle larvae. Based on the physiological and molecular similarities to B. thuringiensis subsp. tenebrionis, it is suggested that the B. thuringiensis SR6 and SR8 may be mutants of the B. thuringiensis subsp. tenebrionis strain overexpressing the crystal of 70 kDa toxin protein.

• Molecules and Cells
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• v.45 no.7
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• pp.495-501
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• 2022

Leucine dehydrogenase (LDH, EC 1.4.1.9) catalyzes the reversible deamination of branched-chain L-amino acids to their corresponding keto acids using NAD⁺ as a cofactor. LDH generally adopts an octameric structure with D4 symmetry, generating a molecular mass of approximately 400 kDa. Here, the crystal structure of the LDH from Pseudomonas aeruginosa (Pa-LDH) was determined at 2.5 Å resolution. Interestingly, the crystal structure shows that the enzyme exists as a dimer with C2 symmetry in a crystal lattice. The dimeric structure was also observed in solution using multiangle light scattering coupled with size-exclusion chromatography. The enzyme assay revealed that the specific activity was maximal at 60℃ and pH 8.5. The kinetic parameters for three different amino acid and the cofactor (NAD⁺) were determined. The crystal structure represents that the subunit has more compact structure than homologs' structure. In addition, the crystal structure along with sequence alignments indicates a set of non-conserved arginine residues which are important in stability. Subsequent mutation analysis for those residues revealed that the enzyme activity reduced to one third of the wild type. These results provide structural and biochemical insights for its future studies on its application for industrial purposes.

• The Korea Journal of Herbology
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• v.20 no.4
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• pp.77-82
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• 2005

Objectives : In order to distinguish Akebiae Caulis and Aristoiochiae Manshuriensis Caulis, their external and internal states were examined by microscope. Methods : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Results : 1. Akebiae Caulis has stone cells and fiber bundles in its pericycle part, which include square crystal of calcium oxalate. Outside of neogenesis has a lot of cells with square crystal of calcium oxalate. 2. Aristoiochiae Manshuriensis Caulis has a little lignified fiber bundles in its pericycle part, and parenchymatous cell has delicate starch grains and druse of calcium oxalate. Conclusions : In conclusion, internal forms of Akebiae Caulis and of Aristoiochiae Manshuriensis Caulis are different in that each includes square crystal of calcium oxalate and druse of calcium oxalate respectively, so that it may be possible to distinguish them with optical microscope.

• Molecules and Cells
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• v.27 no.6
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• pp.667-671
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• 2009

Visfatin (Nampt/PBEF) plays a pivotal role in the salvage pathway for $NAD^+$ biosynthesis. Its potent inhibitor, FK866, causes cellular $NAD^+$ levels to decline, thereby inducing apoptosis in tumor cells. In an effort to improve the solubility and binding interactions of FK866, we designed and synthesized IS001, in which a ribose group is attached to the FK866 pyridyl ring. Here, we report the crystal structure of rat visfatin in complex with IS001. Like FK866, IS001 is positioned at the dimer interface, and all of the residues that interact with IS001 are involved in hydrophobic or ${\pi}-{\pi}$-stacking interactions. However, we were unable to detect any strong interactions between the added ribose ring of IS001 and visfatin, which implies that a bulkier modifying group is necessary for a tight interaction. This study provides additional structure-based information needed to optimize the design of visfatin inhibitors.