• 셀메드
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• 제4권4호
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• pp.26.1-26.9
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• 2014

Reactive oxygen species are known to mediate various pathological conditions associated with malaria. In this study, the antioxidant potential of Clerodendrum violaceum leaf extracts, an indigenous antimalarial remedy, was evaluated. Total phenol, flavonoid, selenium, vitamins C and E contents of Clerodendrum violaceum leaf extracts were determined. The free radical scavenging activities of the extracts against DPPH, superoxide anion and hydrogen peroxide coupled with their reducing power were also evaluated in vitro. Moreover, responses of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) in a rodent malaria model to a 4-day administration of Clerodendrum violaceum leaf extracts were also evaluated. The methanolic extract was found to contain the highest amounts of antioxidant compounds/element and also demonstrated the highest free radical scavenging activity in vitro. The results showed a significant decrease (p < 0.05) in SOD and CAT activities with a concurrent significant (p < 0.05) increase in GPx and GR activities in both erythrocytes and liver of untreated Plasmodium berghei NK65-infected animals compared to the uninfected animals. The extracts were able to significantly increase (p < 0.05) SOD and CAT activities and significantly reduce (p < 0.05) GPx and GR activities in both the liver and erythrocytes compared to those observed in the untreated infected animals. The results suggest the augmentation of the antioxidant system as one of the possible mechanisms by which Clerodendrum violaceum extract ameliorates secondary effects of malaria infection, alongside its antiplasmodial effect in subjects.

• Steel and Composite Structures
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• 제19권3호
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• pp.661-678
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• 2015

Buckling Restrained Braces (BRB) have been widely used in the construction industry as they utilize the most desirable properties of both constituent materials, i.e., steel and concrete. They present excellent structural qualities such as high load bearing capacity, ductility, energy-absorption capability and good structural fire behaviour. The effects of size and type of filler material in the existed gap at the steel core-concrete interface as well as the element's cross sectional shape, on BRB's fire resistance capacity was investigated in this paper. A nonlinear sequentially-coupled thermal-stress three-dimensional model was presented and validated by experimental results. Variation of the samples was described by three groups containing, the steel cores with the same cross section areas and equal yield strength but different materials (metal and concrete) and sizes for the gap. Responses in terms of temperature distribution, critical temperature, heating elapsed time and contraction level of BRB element were examined. The study showed that the superior fire performance of BRB was obtained by altering the filler material in the gap from metal to concrete as well as by increasing the size of the gap. Also, cylindrical BRB performed better under fire conditions compared to the rectangular cross section.

• Journal of Microbiology
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• 제44권2호
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• pp.145-154
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• 2006

Heterotrimeric G proteins (G proteins) are conserved in all eukaryotes and are crucial components sensing and relaying external cues into the cells to elicit appropriate physiological and biochemical responses. Basic units of the heterotrimeric G protein signaling system include a G protein-coupled receptor (GPCR), a G protein composed of ${\alpha},\;{\beta},\;and\;{\gamma}$ subunits, and variety of effectors. Sequential sensitization and activation of these G protein elements translates external signals into gene expression changes, resulting in appropriate cellular behaviors. Regulators of G protein signaling (RGSs) constitute a crucial element of appropriate control of the intensity and duration of G protein signaling. For the past decade, G protein signaling and its regulation have been intensively studied in a number of model and/or pathogenic fungi and outcomes of the studies provided better understanding on the upstream regulation of vegetative growth, mating, development, virulence/pathogenicity establishment, and biosynthesis of secondary metabolites in fungi. This review focuses on the characteristics of the basic upstream G protein components and RGS proteins, and their roles controlling various aspects of biological processes in the model filamentous ascomycete fungus Aspergillus nidulans. In particular, their functions in controlling hyphal proliferation, asexual spore formation, sexual fruiting, and the mycotoxin sterigmatocystin production are discussed.

• Journal of Microbiology and Biotechnology
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• 제24권4호
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• pp.568-576
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• 2014

TIM-1 (also known as KIM-1 and HAVcr-1) is a type I transmembrane glycoprotein member of the TIM family that may play important roles in innate and adaptive immune responses. The overexpression of proteins associated with membrane proteins is a major obstacle to overcome in studies of membrane protein structures and functions. In this study, we successfully coupled the overexpression of the TIM-1 protein with a C-terminal enhanced green fluorescent protein (GFP) tag in Escherichia coli. To the best of our knowledge, this report is the first to describe the overexpression of human TIM-1 in E. coli. The purified TIM-1-EGFP fusion protein recognized and bound directly to apoptotic cells and did not to bind to viable cells. Furthermore, we confirmed that the interactions of TIM-1-EGFP with apoptotic cells were blocked by TIM-1-Fc fusion proteins. This fusion protein represents a readily obtainable source of biologically active TIM-1 that may prove useful in future studies of human TIM-1.

• 소음진동
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• 제5권2호
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• pp.225-234
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• 1995

In this study, first, transformation process of damping ratios, whose are evaluated in active control analysis, into damping matrix resulting from installed viscous dampers is illustrated. Then, a method is followed to maximize the effect of response reduction, which leads to optimum locations and size of viscous dampers using sensitivity analysis. Highly coupled nonlinearity between damping ratios and dampers makes it hard to find the optimal size of dampers. Therefore, the nonlinearity is transformed to linear problem with small increments of damping ratios and the size of dampers can be found. However, there are many cases for the size of dampers satisfying the small increment of damping ratios, so it is necessary to select minimum size using optimization technique. To determine optimum locations of dampers, dampers are assumed to be installed between the different stories and their locations are selected corresponding corresponding to the degree of damping size. Numerical examples for the frame structure and the shear wall structure show that optimum locations and size of dampers are different form each other depending on the characteristics of modal responses of the structures. The proposed method in this study can be applied to get optimum locations of active controller in the active control.

• Wind and Structures
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• 제6권1호
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• pp.53-70
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• 2003

Tsing Ma Bridge in Hong Kong is the longest suspension bridge in the world carrying both highway and railway. It has two H-shape concrete towers, each of which is composed of two reinforced concrete legs and four deep transverse prestressed concrete beams. A series of wind tunnel tests have been performed to measure the aerodynamic coefficients of the tower legs and transverse beams in various arrangements. A 1:100 scaled 3D rigid model of the full bridge tower assembled from various tower components has been constructed for different test cases. The aerodynamic coefficients of the lower and upper segments of the windward and leeward tower legs and those of the transverse beams at different levels, with and without the dummy bridge deck model, were measured as a function of yaw wind angle. The effects of wind interference among the tower components and the influence of the bridge deck on the tower aerodynamic coefficients were also investigated. The results achieved can be used as the pertinent data for the comparison of the computed and field-measured fully coupled buffeting responses of the entire bridge under yaw winds.

• Earthquakes and Structures
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• 제3권5호
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• pp.767-781
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• 2012

The residual capacity against collapse of a main shock-damaged bridge can be coupled with the aftershock ground motion hazard to make an objective decision on its probability of collapse in aftershocks. In this paper, a steel tower suspension bridge with a main span of 2000 m is adopted for a case-study. Seismic responses of the bridge in longitudinal and transversal directions are analyzed using dynamic elasto-plastic finite displacement theory. The analysis is conducted in two stages: main shock and aftershocks. The ability of the main shock-damaged bridge to resist aftershocks is discussed. Results show that the damage caused by accumulated plastic strain can be ignored in the long-span suspension bridge. And under longitudinal and transversal seismic excitations, the damage is prone to occur at higher positions of the tower and the shaft-beam junctions. When aftershocks are not large enough to cause plastic strain in the structure, the aftershock excitation can be ignored in the seismic damage analysis of the bridge. It is also found that the assessment of seismic damage can be determined by superposition of damage under independent action of seismic excitations.

• Molecules and Cells
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• 제41권2호
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• pp.150-159
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• 2018

Animals use their odorant receptors to receive chemical information from the environment. Insect odorant receptors differ from the G protein-coupled odorant receptors in vertebrates and nematodes, and very little is known about their protein-protein interactions. Here, we introduce a mass spectrometric platform designed for the large-scale analysis of insect odorant receptor protein-protein interactions. Using this platform, we obtained the first Orco interactome from Drosophila melanogaster. From a total of 1,186 identified proteins, we narrowed the interaction candidates to 226, of which only two-thirds have been named. These candidates include the known olfactory proteins Or92a and Obp51a. Around 90% of the proteins having published names likely function inside the cell, and nearly half of these intracellular proteins are associated with the endomembrane system. In a basic loss-of-function electrophysiological screen, we found that the disruption of eight (i.e., Rab5, CG32795, Mpcp, Tom70, Vir-1, CG30427, Eaat1, and CG2781) of 28 randomly selected candidates affects olfactory responses in vivo. Thus, because this Orco interactome includes physiologically meaningful candidates, we anticipate that our platform will help guide further research on the molecular mechanisms of the insect odorant receptor family.

• Coupled systems mechanics
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• 제3권2호
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• pp.195-211
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• 2014

When strong seismic forces act on reinforced concrete structures, their beam-column connections are very susceptible to damage during the earthquake event. The aim of this numerical work is to evaluate the influence of the internal steel reinforcement array on the nonlinear response of a RC beam-column connection when it is subjected to strong cyclic loading -as a seismic load. For this, two specimens (extracted from an experimental test of 12 RC beam-column connections reported in literature) were modeled in the Finite Element code FEAP considering different stirrup's arrays. In order to evaluate the nonlinear response of the RC beam-column connection, the 2D model takes into account the nonlinear thermodynamic behavior of each component: for concrete, a damage model is used; for steel reinforcement, it is adopted a classical plasticity model; in the case of the steel-concrete bonding, this one is considered perfect without degradation. At the end, we show a comparison between the experimental test's responses and the numerical results, which includes the distribution of shear stresses and damage inside the concrete core of the beam-column connection; in the other hand, the effects on the connection of a low and high state of confinement are analyzed for all cases.

• Journal of Microbiology and Biotechnology
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• 제26권6호
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• pp.1124-1131
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• 2016

In this study, the global proteome of the IPEC-J2 cell line was evaluated using ultra-high performance liquid chromatography coupled to a quadrupole Q Exactive^™ Orbitrap mass spectrometer. Proteins were isolated from highly confluent IPEC-J2 cells in biological replicates and analyzed by label-free mass spectrometry prior to matching against a porcine genomic dataset. The results identified 1,517 proteins, accounting for 7.35% of all genes in the porcine genome. The highly abundant proteins detected, such as actin, annexin A2, and AHNAK nucleoprotein, are involved in structural integrity, signaling mechanisms, and cellular homeostasis. The high abundance of heat shock proteins indicated their significance in cellular defenses, barrier function, and gut homeostasis. Pathway analysis and annotation using the Kyoto Encyclopedia of Genes and Genomes database resulted in a putative protein network map of the regulation of immunological responses and structural integrity in the cell line. The comprehensive proteome analysis of IPEC-J2 cells provides fundamental insights into overall protein expression and pathway dynamics that might be useful in cell adhesion studies and immunological applications.