• 한국해양공학회지
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• 제23권6호
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• pp.61-66
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• 2009

For a spread-moored FPSO (Floating Production, Storage, & Off-loading) subjected to environmental excitation from waves, current, and wind, a procedure to determine optimum length and stiffness of mooring lines is suggested using quasi-static frequency domain response analyses. Coupled relations between design parameters are closely examined. In consideration of this, optimized design parameters are proposed based on minimum weight condition. The initial design parameters for numerical analyses are calculated using the static catenary equation of mooring lines. It is demonstrated the line tension and vessel's offset are influenced by the mooring line length and stiffness. Accordingly it is suggested the optimum vessel's offset range should be determined considering line fatigue damage. The availability and limitation of the application of quasi-static analysis method for spread mooring system are explained by comparing the result of time domain analysis with one of frequency domain analysis.

• 한국작물학회지
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• 제62권1호
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• pp.40-50
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• 2017

Seed storage proteins are used as carbon and nitrogen sources for the nutritional improvement of seeds. Since the composition of proteins from the Korean cultivars of proso millet is unknown, this study was conducted to obtain a reference map of millet seed proteins and identify the functional characteristics of the identified proteins. Proteins extracted from proso millet seeds of various cultivars were investigated using proteomic techniques such as 2-D electrophoresis coupled with mass fingerprinting; 1152 (differentially expressed) protein spots were detected on the 2-D gels. Among them, 26 reproducible protein spots were analyzed using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry. Out of the 26 proteins, 2 proteins were upregulated in all the millet cultivars, while 13 proteins were upregulated and 11 proteins were downregulated in 2 cultivars. Abundance of most of the identified protein species associated with polysaccharide and starch metabolism, transcription, and pathogenesis was significantly enhanced, while that of other protein species involved in glycolysis, stress response, and transduction was severely reduced. Taken together, the results suggest that the differential expression of the proteins from the four millet cultivars may be cultivar-specific. By conducting a proteomic investigation of millet seeds from different cultivars, we sought to better understand the functional categorization of individual proteins on the basis of their molecular functions. We believe that the identified proteins may help in investigating genetic variations in millet cultivars.

• 한국항공운항학회지
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• 제9권1호
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• pp.59-69
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• 2001

복합재로 된 회전날개깃을 상자보로 모델링하고 수동/능동 감쇠를 주기 위해 ACL(Active Constrained Damping Layer)을 상하양면에 부착하고 복합변위이론에 기초한 유한요소방법을 이용하여 구조해석을 수행하였다. 이 이론은 ACL내의 복합재와 점탄성층 그리고 압전층의 전단변형효과를 정확하게 모델링하는데 효과적이다. Hankel 의 특이값을 이용해 축차모델을 유도하였으며 축차모델과 측정된 출력에 기초한 LQG 제어기를 설계하였다. 그러나 LQG 제어기는 공칭 운전속도에서는 좋은 성능을 보여주었으나 운전속도가 변하는 상황에 대해서는 강인안정성을 보여주지 못했다. 이 LQG제어기의 강인안정성을 개선하기 위하여 루프전달회복을 통한 강인한 제어기를 설계하였다. 수치 예를 통해 제시된 제어기가 회전날개깃의 공기역학적인 안정성을 개선하는데 효과적이며 동체모드와 연계된 리드-래그 모드감쇠를 증가시켜 회전날개깃의 진동을 효과적으로 억제하는 것을 보였다.

• Coupled systems mechanics
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• 제1권4호
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• pp.381-395
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• 2012

Dynamic response of Pile Supported Structures is highly depended on Soil Pile Structure Interaction. In this paper, by comparison of experimental and numerical dynamic responses of a prototype jacket offshore platform for both hinge based and pile supported boundary conditions, effect of soil-pile-structure interaction on dynamic characteristics of this platform is studied. Jacket and deck of a prototype platform is installed on a hinge-based case first and then platform is installed on eight skirt piles embedded on continuum monolayer sand. Dynamic characteristics of platform in term of natural frequencies, mode shapes and modal damping are compared for both cases. Effects of adding and removing vertical bracing members in top bay of jacket on dynamic characteristics of platform for both boundary conditions are also studied. Numerical simulation of responses for the studied platform is also performed for both mentioned cases using capability of ABAQUS and SACS software. The 3D model using ABAQUS software is created using solid elements for soil and beam elements for jacket, deck and pile members. Mohr-Coulomb failure criterion and pile-soil interface element are used for considering nonlinear pile soil structure interaction. Simplified modeling of soil-pile-structure interaction effect is also studied using SACS software. It is observed that dynamic characteristics of the system changes significantly due to soil-pile-structure interaction. Meanwhile, both of complex and simplified (ABAQUS and SACS, respectively) models can predict this effect accurately for such platforms subjected to dynamic loading in small range of deformation.

• Coupled systems mechanics
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• 제2권4호
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• pp.329-348
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• 2013

This study aims at observing the coupling behaviours between suspended ceilings and partition walls in terms of their global seismic performance using full-scale shake table tests. The suspended ceilings with planar dimensions of $6.0m{\times}3.6m$ were tested with two types of panels: acoustic lay-in and metal clip-on panels. They were further categorized as seismic-braced, seismic-unbraced, and non-seismic installations. Also, two configurations of 2.7 m high partition wall specimens, with C-shape and I-shape in the plane layouts, were tested. In total, seven ceiling-partition-coupling (CPC) specimens were tested utilizing a unidirectional seismic simulator. The test results indicate that the damage patterns of the tested CPC systems included failure of the ceiling grids, shearing-off of the wall top railing, and, most destructively, numerous partial detachments and falling of the ceiling panels. The loss of panels was mostly concentrated near the center of the tested partition wall. The testing results also confirmed that the failure mode of the non-seismic CPC systems was brittle: The whole system would collapse suddenly all at once when the magnitude of the inputs hit the capacity threshold, rather than displaying progressive damage. Overall, the seismic capacity of the unbraced and braced CPC systems could be up to 1.23 g and 2.67 g, respectively; these accelerations were both achieved at the base of the partition wall. Nonetheless, for practical applications, it is noteworthy that the three-dimensional nature of seismic excitations and the size effect of the ceiling area are parameters that exacerbate the CPC's seismic response so that their actual capacity may be dramatically decreased, leading to important losses even in moderate seismic events.

• 한국산학기술학회논문지
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• 제19권7호
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• pp.16-23
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• 2018

카오스는 비선형 과학 분야에서 매우 중요한 주제 중의 하나이며, Lorenz가 처음으로 소개한 이후 집중적으로 연구되어지고 있다. 카오스 시스템의 한 특성은 카오스 시스템에 의해 생성된 신호는 다른 어떤 시스템과 동기화되지 않는다는 것이다. 따라서 두 카오스 시스템은 서로 동기화되는 것이 불가능한 것처럼 보이지만, 만약 두 시스템이 적절한 방법으로 정보를 교환한다면 이 두 시스템은 동기화가 가능하다. 본 논문에서는 능동 제어와 슬라이딩 모드 제어, 그리고 리아프노프 안정도 이론을 기반으로 하는 변형된 Lorenz 카오스 시스템의 동기화 문제에 대해 다룬다. 동기화를 위해 고려한 기법은 선형상태 오차 변수에 의해 짝을 이룬 구동시스템과 응답시스템으로 구성된다. 이를 위해 우선 대상 카오스 시스템에 대해 간단히 살펴본다. 다음으로 능동제어, 슬라이딩 모드 제어 기법을 이용한 카오스 시스템의 동기화와 채터링 문제를 해결하기 위한 제어 방법을 도출한다. 전체 폐루프 시스템의 점근적 안정도는 리아프노프 안정도 이론에 의해 증명한다. 컴퓨터 시뮬레이션은 제안한 방법의 타당성을 확인하기 위해 그래픽으로 제시한다.

• Earthquakes and Structures
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• 제6권1호
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• pp.71-87
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• 2014

Interaction between closely-spaced buildings subject to earthquake induced strong ground motions, termed in the literature as "seismic pounding", occurs commonly during major seismic events in contemporary congested urban environments. Seismic pounding is not taken into account by current codes of practice and is rarely considered in practice at the design stage of new buildings constructed "in contact" with existing ones. Thus far, limited research work has been devoted to quantify the influence of slab-to-slab pounding on the inelastic seismic demands at critical locations of structural members in adjacent structures that are not aligned in series. In this respect, this paper considers a typical case study of a "new" reinforced concrete (R/C) EC8-compliant, torsionally sensitive, 7-story corner building constructed within a block, in bi-lateral contact with two existing R/C 5-story structures with same height floors. A non-linear local plasticity numerical model is developed and a series of non-linear time-history analyses is undertaken considering the corner building "in isolation" from the existing ones (no-pounding case), and in combination with the existing ones (pounding case). Numerical results are reported in terms of averages of ratios of peak inelastic rotation demands at all structural elements (beams, columns, shear walls) at each storey. It is shown that seismic pounding reduces on average the inelastic demands of the structural members at the lower floors of the 7-story building. However, the discrepancy in structural response of the entire block due to torsion-induced, bi-directionally seismic pounding is substantial as a result of the complex nonlinear dynamics of the coupled building block system.

• Journal of Microbiology and Biotechnology
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• 제18권2호
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• pp.248-254
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• 2008

Candida magnoliae, an osmotolerant and erythritol producing yeast, prefers D-fructose to D-glucose as carbon sources. For the investigation of the fructophilic characteristics with respect to sugar transportation, a sequential extraction method using various detergents and ultracentrifugation was developed to isolate cellular membrane proteins in C. magnoliae. Immunoblot analysis with the Pma1 antibody and two-dimensional electrophoresis analysis coupled with MS showed that the fraction II was enriched with membrane proteins. Eighteen proteins out of 36 spots were identified as membrane or membrane-associated proteins involved in sugar uptake, stress response, carbon metabolism, and so on. Among them, three proteins were significantly upregulated under the fructose supplying conditions. The hexose transporter was highly homologous to Ght6p in Schizosaccharomyces pombe, which was known as a predominant transporter for the fructose uptake of S. pombe because it exhibited higher affinity to D-fructose than D-glucose. The physicochemical properties of the ATP-binding cassette transporter and inorganic transporter explained their direct or indirect associations with the fructophilic behavior of C. magnoliae. The identification and characterization of membrane proteins involved in sugar uptake might contribute to the elucidation of the selective utilization of fructose to glucose by C. magnoliae at a molecular level.

• The Korean Journal of Physiology and Pharmacology
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• 제17권2호
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• pp.139-147
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• 2013

Lysolipids such as LPA, S1P and SPC have diverse biological activities including cell proliferation, differentiation, and migration. We investigated signaling pathways of LPA-induced contraction in feline esophageal smooth muscle cells. We used freshly isolated smooth muscle cells and permeabilized cells from cat esophagus to measure the length of cells. Maximal contraction occurred at $10^{-6}M$ and the response peaked at 30s. To identify LPA receptor subtypes in cells, western blot analysis was performed with antibodies to LPA receptor subtypes. LPA1 and LPA3 receptor were detected at 50 kDa and 44 kDa. LPA-induced contraction was almost completely blocked by LPA receptor (1/3) antagonist KI16425. Pertussis toxin (PTX) inhibited the contraction induced by LPA, suggesting that the contraction is mediated by a PTX-sensitive G protein. Phospholipase C (PLC) inhibitors U73122 and neomycin, and protein kinase C (PKC) inhibitor GF109203X also reduced the contraction. The PKC-mediated contraction may be isozyme-specific since only $PKC{\varepsilon}$ antibody inhibited the contraction. MEK inhibitor PD98059 and JNK inhibitor SP600125 blocked the contraction. However, there is no synergistic effect of PKC and MAPK on the LPA-induced contraction. In addition, RhoA inhibitor C3 exoenzyme and ROCK inhibitor Y27632 significantly, but not completely, reduced the contraction. The present study demonstrated that LPA-induced contraction seems to be mediated by LPA receptors (1/3), coupled to PTX-sensitive G protein, resulting in activation of PLC, PKC-${\varepsilon}$ pathway, which subsequently mediates activation of ERK and JNK. The data also suggest that RhoA/ROCK are involved in the LPA-induced contraction.

• Smart Structures and Systems
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• 제17권3호
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• pp.471-489
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• 2016

Modal identification based on ambient vibration data has attracted extensive attention in the past few decades. Since the excitation for ambient vibration tests is mainly from the environmental effects such as wind and traffic loading and no artificial excitation is applied, the signal to noise (s/n) ratio of the data acquired plays an important role in mode identifiability. Under ambient vibration conditions, certain modes may not be identifiable due to a low s/n ratio. This paper presents a study on the mode identifiability of an instrumented cable-stayed bridge with the use of acceleration response data measured by a long-term structural health monitoring system. A recently developed fast Bayesian FFT method is utilized to perform output-only modal identification. In addition to identifying the most probable values (MPVs) of modal parameters, the associated posterior uncertainties can be obtained by this method. Likewise, the power spectral density of modal force can be identified, and thus it is possible to obtain the modal s/n ratio. This provides an efficient way to investigate the mode identifiability. Three groups of data are utilized in this study: the first one is 10 data sets including six collected under normal wind conditions and four collected during typhoons; the second one is three data sets with wind speeds of about 7.5 m/s; and the third one is some blind data. The first two groups of data are used to perform ambient modal identification and help to estimate a critical value of the s/n ratio above which the deficient mode is identifiable, while the third group of data is used to perform verification. A couple of fundamental modes are identified, including the ones in the vertical and transverse directions respectively and coupled in both directions. The uncertainty and s/n ratio of the deficient mode are investigated and discussed. A critical value of the modal s/n ratio is suggested to evaluate the mode identifiability of the deficient mode. The work presented in this paper could provide a base for the vibration-based condition assessment in future.