• KSBB Journal
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• 제13권4호
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• pp.456-460
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• 1998

Fed-batch fermentations were carried out in order to improve the efficiency of hirudin production by recombinant Saccharomyces cerevisiae. A fed-batch fermentation done with the optimized semi-synthetic medium resulted in a maximum hirudin concentration of 342mg/$\ell$ by keeping a galactose concentrations between 10 and 30g/$\ell$ which corresponded to a 11.4-fold increase in hirudin concentration compared with the simple bach fermentation done with the same medium. Comparison of the chromatographic pattern of proteins in the growth medium clearly showed that the use of the semi-synthetic medium is more advantageous for separation of hirudin than the case o fusing the complex medium. Continuous cell recycle fermentation done at dilution rate of 0.1h-1 and an inlet galactose concentration of 100g/$\ell$ yielded a maximum hirudin productivity of 19.1mg hirudin/$\ell$$.$h.

• 대한안전경영과학회지
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• 제24권3호
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• pp.93-103
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• 2022

This study investigated the method of continuous improvement of small-medium company production processes through POSCO's QSS(Quick Six Sigma) activities. QSS is a field operation technique that encompasses the advantages of Six Sigma, TPS(Toyota Production System), TQM (Total Quality Management), and IE(Industrial Engineering). Through this, POSCO not only encourages activities centered on related small and medium-sized partners, etc., but is also expected to contribute to the continuous improvement of the company's own production process through QSS activities. In this study, rather than unconditionally carrying out activities according to the needs of large companies, the research is to help the continuous improvement of the actual production process of small and medium-sized enterprises by effectively applying and spreading QSS activities in consideration of the characteristics and environment of the company. For this purpose, empirical research is conducted on the process improvement activities and QSS activities of company Y, which has less than 100 assembly and production quality and inspection processes among SMEs. The changes in the production process improvement of SMEs through the application of the final QSS were investigated through empirical studies.

• KSBB Journal
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• 제8권1호
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• pp.42-48
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• 1993

Continuous degradation of formaldehyde by using a rotating disc contactor was investigated in this study. Peudomonas putida H-5K was selected as a mutant using N-methyl N'-nitro N-nitrosoguandine (250$\mu\textrm{g}$/$m\ell$), which showed 1.5 times higher ability of formaldehyde degradation than that of the parent strain. Enzyme activity for formaldehyde degradation released form Peudomonas putida H-5K showed the highest level of 6.2mo1/min/mg protein in the 2% glucose mineral medium containing 0.02% formaldehyde. Degradation of formaldehyde from the first stage in rotating disc contactor was 95% and 5% from the 4th stage when the reactor was fed with 0.02% or 0.04% formaldehyde solution at a rate of 20$m\ell$ per hour. Continuous degradation of formaldehyde using rotating disc contactor was above 95%o in the medium containing 0.04% formalchyde, at the medium feed ratc of 20$m\ell$ per hour.

• 한국미생물·생명공학회지
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• 제27권1호
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• pp.46-53
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• 1999

The purpose of this study was to optimize the conditions of continuous mixed culture of C.butyricum and R. spaeroides K-7, which were able to produce hydrogen using biomass-dreived substrate. To investigate the possibility of continuous culture, semi-continuous culture was carried out for 20 days. In semi-continuous culture using the reactor system, the replacement rate of fresh medium was 30% of total medium volume for the highest hydrogen evolution. In continuous culture, the optimum dilution rate was determined to be 0.05$h^{-1}$. The continuous culture produced 3.1 times as compared with the hydrogen on batch culture. On the other hand, the continuous mixed culture produced 1.3~2.1 times as much as hydrogen of the continuous monoculture of C. butyricum. When 10g of glucose in the media (1l) was supplied as a carbon source on continuous culture, mixed culture of C. butyricum and R. sphaeroides K-7 increased hydrogen evolution rate. Because considerable amount of glutamate was contained in waste water of glutamate fermentation, utilization of glutamate was examined in mixed culture. As a result of examination, production of hydorgen was slightly inhibited by high concentration of glutamate, more than 20mM, on continuous monoculture of R. sphaeroides K-7. On the other hand, both on continuous monoculture of C. butyricum and on mixed culture of C. butyricum and R. sphaeroides K-7, production of hydrogen was not inhibited by high concentration of glutamate such as 100mM. Hence this suggests that high concentration of waste water can be used as good substrate for hydrogen production on monoculture of C. butyricum and mixed culture of C. butyricum and R. sphaeroides K-7.

• 미생물학회지
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• 제15권2호
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• pp.63-76
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• 1977

Irradiation with high doses of gamma rays induced the reduction of mycelial weight and anaylase activity, and increased relative amylase activity in surface cultures. Biphase in growth curves was shown in aeration-agitation cultures but the behavior of the first phase of growth could be eliminated by replacing the amylasehydrolysed starch substrates, so that enzyme production was shortened ca. 40 hours and relative amylase activity was increased about 3 times higher before onset of autolysis. In the effect of gibberellin on amylase production, the positive stimulation was appeared to only surface culturs of the liquid medium and the negative effect to shake-cultures in a mutant. Trials of various continuous culture were resulted not only the approalch to the value of amylase activity in surface cultures of liquid medium, but also higher productivity than in batch cultures. The culture-degeneration was observed in two-stage continuous culture, but did not appear in continuous elevation culture.

• Journal of Microbiology and Biotechnology
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• 제2권4호
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• pp.284-287
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• 1992

Repeated-batch and continuous cultures of Lactococcus sp. 1112-1 were carried out for bacteriocin production using a glucose-casein medium. Repeated-batch culture did not efficiently enhanced the bacteriocin production. Continuous production was possible at the dilution rate of 0.4 $h^{-1}$. Maximum specific production rate ($Q^p$), bacteriocin production and biomass at the dilution rate were 347, 136 IU/g/h, 2, 121 IU/ml and 2.45 g/L, respectively.

• Smart Structures and Systems
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• 제20권5호
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• pp.525-537
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• 2017

This paper explores different vibration control strategies for the cancellation of human-induced vibration on a structure with time-varying modal parameters. The main motivation of this study is a lively urban stress-ribbon footbridge (Pedro $G\acute{o}mez$ Bosque, Valladolid, Spain) that, after a whole-year monitoring, several natural frequencies within the band of interest (normal paring frequency range) have been tracked. The most perceptible vibration mode of the structure at approximately 1.8 Hz changes up to 20%. In order to find a solution for this real case, this paper takes the annual modal parameter estimates (approx. 14000 estimations) of this mode and designs three control strategies: a) a tuned mass damper (TMD) tuned to the most-repeated modal properties of the aforementioned mode, b) two semi-active TMD strategies, one with an on-off control law for the TMD damping, and other with frequency and damping tuned by updating the damper force. All strategies have been carefully compared considering two structure models: a) only the aforementioned mode and b) all the other tracked modes. The results have been compared considering human-induced vibrations and have helped the authors on making a decision of the most advisable strategy to be practically implemented.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권2호
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• pp.115-121
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• 2005

Using a general Saccharomyces cerevisiae as a model strain, continuous ethanol fermentation was carried out in a stirred tank bioreactor with a working volume of 1,500 mL. Three different gravity media containing glucose of 120, 200 and 280 g/L, respectively, supplemented with 5 g/L yeast extract and 3 g/L peptone, were fed into the fermentor at different dilution rates. Although complete steady states developed for low gravity medium containing 120 g/L glucose, quasi-steady states and oscillations of the fermented parameters, including residual glucose, ethanol and biomass were observed when high gravity medium containing 200 g/L glucose and very high gravity medium containing 280 g/L glucose were fed at the designated dilution rate of $0.027\;h^{-1}$. The observed quasi-steady states that incorporated these steady states, quasi-steady states and oscillations were proposed as these oscillations were of relatively short periods of time and their averages fluctuated up and down almost symmetrically. The continuous kinetic models that combined both the substrate and product inhibitions were developed and correlated for these observed quasi-steady states.

• 한국미생물·생명공학회지
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• 제22권6호
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• pp.565-570
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• 1994

The pullulan production and morphological change of Aureobasidium pullulans ATCC 9348 were investigated both in batch fermentation and in continuous fermentation. The best carbon source for pullulan production was sucrose among seven different carbon sources. The pullulan production of A. pullulans was increased with increasing the carbon to nitrogen ratio of the medium using sucrose as a carbon source. In batch fermentation, production of pullullan occurred following exhaustion of the nitrogen source from the medium. The continuous fermentation showed that the pullulan production was closely parallelled with cell growth and was most effective at a dilution rate of 0.06~0.07 hr$^{-1}$-. The ratio of yeast-like cells(blastospores) of A. pullulans increased with the increase of growth rate, and reached 100% over the growth rate of 0.07 hr$^{-1}$. The growth rate, within a certain range, affected not only on the cell morphology, but on the specific pullulan productivity of A. pullulans.

• 유체기계공업학회:학술대회논문집
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• 유체기계공업학회 2003년도 유체기계 연구개발 발표회 논문집
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• pp.115-120
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• 2003

In this work, a continuous-flow micro-PCR system is systematically designed. From the numerical simulation based on the finite volume method, adapting oneself to a new environmental temperature without an external temperature controller is shown to be possible and a cooler as well as a heater is shown to be necessary to obtain three individual temperature zones for polymerase chain reaction. In addition, appropriate geometry of a heat sink for the cooler is determined by using a compact modeling method, the porous medium approach.