• 한국환경농학회지
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• 제37권2호
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• pp.146-149
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• 2018

아셀렌산염(selenite, ${SeO_3}^{2-}$)을 환원시키는 세균 SE4-1을 복합 산업단지 내 위치한 오염된 하천 퇴적토로부터 분리하였고, 계통분석결과 이 균주는 Citrobacter freundii와 가장 유사하였다. 이 균주는 혐기적 환경에서 아셀렌산염을 원소상 셀레늄(elemental selenium, $Se^0$)으로 환원시켰고, 그 형상은 구형으로 확인되었다. 이러한 고체상의 침전은 셀레늄 산소음이온의 이동성을 줄이고 생물가용성을 줄여, 생태계 독성을 줄이는 잠재성을 제시해 줄 수 있을 것이다.

• 한국지반환경공학회 논문집
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• 제7권3호
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• pp.43-54
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• 2006

채취된 초기 간극수를 오염물질로 치환시킨 점토시료를 대상으로 일축압축시험, 삼축압축시험, 압밀시험을 수행하여 오염물질 성상에 따른 오염점토의 역학적 특성변화를 분석하였으며, MT3D 해석모델을 이용하여 매립지 성토제방의 점토 차수층을 통한 오염물질의 거동 특성을 파악하였다. NaCl 수용액으로 치환된 시료의 경우, 농도가 증가할수록 강도, 압축성, 투수성이 증가되었다. 침출수로 포화시킨 시료의 경우, NaCl 수용액으로 포화시킨 시료에 비해 강도, 압축성의 증가현상이 크게 나타난 반면 면모화 정도가 큼에도 불구하고 투수계수는 오히려 작게 나타났다. 오염물질 이동 해석결과, 초기 염소이온 농도가 클수록 측정농도도 커지며, 시간경과에 따라 농도는 비선형적으로 증가되었다. 농도감쇄 관계식을 유도하여 이동거리를 예측한 결과, 초기 농도가 클수록 이동거리도 크게 나타났다.

• 한국실내디자인학회논문집
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• 제20권3호
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• pp.182-189
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• 2011

This research suggests a way of hygienic space planing of the customer space in the university student restaurant of E University in Seoul. For this study. the behavior observation in the restaurant was conducted in order to analyze the sequence of customer behavior related space planing applying HACCP (Hazard Analysis And Critical Control Point). Also the survey was conducted to analyze opinions of customers about hygiene of the space. The results of the analyses of the observation and the survey were similar in terms of hygiene. In conclusion, for hygienic space planing of the customer space in restaurant, the space circulation should be planned according to the hygienic sequence of customer behavior. The spaces in restaurant should be divided into two areas: the clean area and the contaminated area to prevent cross-contamination. The clean area, such as spaces preparing dishes and food including water and table hall where customers eat, should be separated from the contaminated area such as the spaces where waste is handled and where dirty dishes are kept. In order to prevent cross-contamination, hygiene facility to remove contamination should be located before going to the clean area. More specifically, the hygiene facility should be located between a cash counter and a space preparing dishes so that customers can wash their hands before they touch dishes. Space arrangement with feed-back process to check the possible points of cross-contamination and correct space arrangement until the possibility of cross-contamination is removed in the restaurant.

• 농약과학회지
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• 제5권4호
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• pp.62-67
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• 2001

붉은곰팡병에 감염된 곡물의 인축에 대한 안전성평가를 위하여 붉은곰팡이병에 30%감염된 밀, 쌀보리 및 겉보리를 마우스먹이에 섞어 먹이는 식이혼입시험을 수행하였다. 붉은곰팡이병에 30% 감염된 밀, 쌀보리 및 겉보리에 대하여 밀은 밀가루로서, 쌀보리와 겉보리는 도정수율을 각각 68% 및 58%로 도정한 것을 마우스사료 중 10, 30, 및 50% 함유되게 사료와 동일한 형태로 조제하여 5주령의 마우스에 2개월 동안 섭식시키면서 영향을 조사하였다. 곡물을 먹이중 혼입비율별로 투여한 모든 군에서 사료섭취량, 음수섭취량 및 체중 등에 영향은 없었다. 또한 임상관찰, 육안적 부검소견, 장기중량 변화, 혈구분별측정 및 혈액생화적 검사 등에서도 모든 투여군에서 이상이 관찰되지 않았다. 이상의 결과에서 본시험에 사용된 가공곡류 중 곰팡이 독소함량으로는 마우스에 대한 영향이 없는 것으로 판단되었다.

• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.169-178
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• 2010

The microbial community structure in Thailand soils contaminated with low and high levels of arsenic was determined by denaturing gradient gel electrophoresis. Band pattern analysis indicated that the bacterial community was not significantly different in the two soils. Phylogenetic analysis obtained by excising and sequencing six bands indicated that the soils were dominated by Arthrobacter koreensis and $\beta$-Proteobacteria. Two hundred and sixty-two bacterial isolates were obtained from arsenic-contaminated soils. The majority of the As-resistant isolates were Gramnegative bacteria. MIC studies indicated that all of the tested bacteria had greater resistance to arsenate than arsenite. Some strains were capable of growing in medium containing up to 1,500 mg/l arsenite and arsenate. Correlations analysis of resistance patterns of arsenite resistance indicated that the isolated bacteria could be categorized into 13 groups, with a maximum similarity value of 100%. All strains were also evaluated for resistance to eight antibiotics. The antibiotic resistance patterns divided the strains into 100 unique groups, indicating that the strains were very diverse. Isolates from each antibiotic resistance group were characterized in more detail by using the repetitive extragenic palindromic-PCR (rep-PCR) DNA fingerprinting technique with ERIC primers. The PCR products were analyzed by agarose gel electrophoresis. The genetic relatedness of 100 bacterial fingerprints, determined by using the Pearson product-moment similarity coefficient, showed that the isolates could be divided into four clusters, with similarity values ranging from 5-99%. Although many isolates were genetically diverse, others were clonal in nature. Additionally, the arsenic-resistant isolates were examined for the presence of arsenic resistance (ars) genes by using PCR, and 30% of the isolates were found to carry an arsenate reductase encoded by the arsC gene.

• 한국수산과학회지
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• 제42권3호
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• pp.238-242
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• 2009

Recently coastal seawater has been contaminated by enteric viruses such as the norovirus via untreated groundwater globally. Accordingly, the consumption of molluscan shellfish from seawater that has been contaminated with fecal material has become an important issues. The levels of enteric viruses in seawater are low and recovery and concentration of the virus from large volumes of water is difficult. We compared the effectiveness of two representative method of concentrating virus using negatively and positively charged filters. The mean retention of seeded FCV by HAMF and NCCF was 48% and 78%, respectively. Overall, the recovery of NCCF was 43.3$\pm$11% better than that of HAMF. However, the eluate obtained by using beef extract solution in the NCCF procedure caused an inhibitory effect on the RT-PCR; therefore, it was necessary to employ a PCR inhibitor removal procedure. The HAMF eluate contained no PCR inhibitors, but HAMF was not an effective method of concentrating the virus from large volumes of natural seawater due to clogging.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권3호
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• pp.205-210
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• 2006

The widespread use and distribution of chloroethylene organic compounds is of serious concern owing to their carcinogenicity and toxicity to humans and wildlife. In an effort to develop active bacterial consortia that could be useful for bioremediation of chloroethylene-contaminated sites in Africa, 16 combinations of 5 dichloroethylene (DCE)-utilizing bacteria, isolated from South Africa and Nigeria, were assessed for their ability to degrade cis- and trans- DCEs as the sole carbon source. Three combinations of these isolates were able to remove up to 72% of the compounds within 7 days. Specific growth rate constants of the bacterial consortia ranged between 0.465 and $0.716\;d^{-1}$ while the degradation rate constants ranged between 0.184 and $0.205\;d^{-1}$ with $86.36{\sim}93.53\;and\;87.47{\sim}97.12%$ of the stoichiometric-expected chloride released during growth of the bacterial consortia in cis- and trans-DCE, respectively. Succession studies of the individual isolates present in the consortium revealed that the biodegradation process was initially dominated by Achromobacter xylosoxidans and subsequently by Acinetobacter sp. and Bacillus sp., respectively. The results of this study suggest that consortia of bacteria are more efficient than monocultures in the aerobic biodegradation of DCEs, degrading the compounds to levels that are up to 60% below the maximum allowable limits in drinking water.

• 한국환경농학회:학술대회논문집
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• 한국환경농학회 2009년도 정기총회 및 국제심포지엄
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• pp.93-115
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• 2009

There are growing public concerns over crop and food safeties due to the elevated levels of heavy metals grown in contaminated soil. Heavy metals are classified as the chemical harmful risks for crop and food safety. With implementation of GAP, crop safety is controlled by many regulatory options for soil, irrigation water and fertilizers. Any attempt to retard the metal uptake by crops may be the best protocol to secure crop and food safety. This article reviews the management strategies for heavy metals in view of crop safety in Korea and demonstrates results from the field experiments to retard metal translocation from soil to crops by using chemical amendments and soil layer management methods. Major source of soil pollution by heavy metals has been related with mining activities. Risk assessment revealed that rice consumption and groundwater ingestion in the abandoned mining areas were the major exposure pathways for metals to human and the heavy metal showed the toxic effects on human health. Chemical amendments such as lime and slag retarded Cd uptake by rice (Oryza sativa L.) by increasing soil pH, lowering the phytoavailable Cd concentration in soil solution, immobilizing Cd in soil and converting the available Cd fractions into non-available fractions. The soil layer management methods decreased the Cd uptake by 76% and Pb by 60%. Either reversing the surface layer with subsurface layer or immobilization of metals with layer mixing with lime was considered to be the practical option for the in-situ remediation of the contaminated paddy soils. Combination of chemical soil amendments and layer management methods was efficient to retard the metal bioavailability and thus to secure crop safety for heavy metals. This protocol seems to be cheap, relatively easy to practice and practical in the agricultural fields. However, a long term monitoring work should be followed to verify the efficiency of this protocol.

• 한국환경과학회지
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• 제19권3호
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• pp.379-387
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• 2010

Sediments of Little Scioto (LS) River in Ohio was contaminated by poor disposal of creosote from Baker Wood Creosoting Facility. Among the primary compounds of creosote, Polycyclic Aromatic Hydrocarbons (PAHs) are the most common ingredient PAHs are known for toxic, carcinogenic and mutagenic compounds. There are many difficulties to remove the PAHs in nature environment because their characteristics are having a less water-solubility, volatile and low mobility properties as increasing the molecular weight. The generation of hydroxyl radicals (${\cdot}OH$) and hydrogen peroxide ($H_2O_2$) forms as well as high temperature (5000 K) and pressure (1000 atm) by a physico-chemical effects of ultrasound during a cavitation collapse can promote the degradation and desorption of PAHs in sediment And it can also produces shock wave and microjets which are able to change the size and surface of particle in solid-liquid system as one of physical effects. Therefore, we explored to understand the role of particle size, the effect of elimination for PAHs concentration by ultrasound and optimize the conditions for ultrasonic treatment. The condition of various size of particles (> $150{\mu}m$, < $150{\mu}m$) and solid-liquid ratio (12.5g/L, 25g/L) for the treatment was considered and ultrasonic power (430 W/L) with liquid - hexane extraction and microwave extraction method were applied after ultrasound treatment.

• 대한환경공학회지
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• 제27권8호
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• pp.892-899
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• 2005

지하수는 지표수에 비하여 수질이 양호하며 외부로부터의 오염원이 적은 편이다. 하지만 현재 우리나라의 지하수도 산업화, 도시화에 의하여 많은 오염이 발생되고 있다. 그 중에서도 PCE와 TCE는 난분해성 염소계 유기화합물로서 그 처리에 신중을 기해야 한다. 본 연구에서는 PAC-막분리 공정을 적용하여 이들 물질을 보다 효과적이면서 컴팩트하게 처리할 수 있었다. 막의 재질에 따른 효율의 차이는 별로 없었으며, PAC가 투과 플럭스의 저하를 유발하지는 않았다. 따라서 PAC의 주입이 효과적으로 이루어진다면 막에서 발생하는 오염을 줄여줄 수가 있으며 보다 효율적인 운전이 이루어 질 수가 있다.