Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Synergistic Utilization of Dichloroethylene as Sole Carbon Source by Bacterial Consortia Isolated from Contaminated Sites in Africa

  • Olaniran, Ademola O. (Department of Microbiology, Faculty of Science and Agriculture, University of KwaZulu-Natal(Westville Campus)) ;
  • Mfumo, Nokukhanya H. (Department of Microbiology, Faculty of Science and Agriculture, University of KwaZulu-Natal(Westville Campus)) ;
  • Pillay, Dorsamy (Department of Microbiology, Faculty of Science and Agriculture, University of KwaZulu-Natal(Westville Campus)) ;
  • Pillay, Balakrishna (Department of Microbiology, Faculty of Science and Agriculture, University of KwaZulu-Natal(Westville Campus))
  • Published : 2006.06.30
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Abstract

The widespread use and distribution of chloroethylene organic compounds is of serious concern owing to their carcinogenicity and toxicity to humans and wildlife. In an effort to develop active bacterial consortia that could be useful for bioremediation of chloroethylene-contaminated sites in Africa, 16 combinations of 5 dichloroethylene (DCE)-utilizing bacteria, isolated from South Africa and Nigeria, were assessed for their ability to degrade cis- and trans- DCEs as the sole carbon source. Three combinations of these isolates were able to remove up to 72% of the compounds within 7 days. Specific growth rate constants of the bacterial consortia ranged between 0.465 and $0.716\;d^{-1}$ while the degradation rate constants ranged between 0.184 and $0.205\;d^{-1}$ with $86.36{\sim}93.53\;and\;87.47{\sim}97.12%$ of the stoichiometric-expected chloride released during growth of the bacterial consortia in cis- and trans-DCE, respectively. Succession studies of the individual isolates present in the consortium revealed that the biodegradation process was initially dominated by Achromobacter xylosoxidans and subsequently by Acinetobacter sp. and Bacillus sp., respectively. The results of this study suggest that consortia of bacteria are more efficient than monocultures in the aerobic biodegradation of DCEs, degrading the compounds to levels that are up to 60% below the maximum allowable limits in drinking water.

Keywords

References

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