• Title/Summary/Keyword: construct

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Growth Performance of Transgenic Mud Loach Misgurnus mizolepis Carrying a GH Transgene Driven by Mud Loach C-Type Lectin Regulator

  • Song, Ha-Yeon;Kim, Dong-Soo
    • Fisheries and Aquatic Sciences
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    • v.15 no.1
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    • pp.43-47
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    • 2012
  • Growth hormone (GH) transgenesis in fish has the potential to improve aquaculture efficiency and capacity. However, many fast-growing transgenic fish have experienced side effects caused by excess GH expression. To overcome this unwanted issue associated with several GH transgenic mud loach Misgurnus mizolepis lines carrying GH construct driven by a strong ${\beta}$-actin regulator ($pml{\beta}$-actGH), we performed an alternative version of GH autotransgenesis using a weaker but more stable regulator, the mud loach lectin promoter. GH transgenesis with a pmlectGH construct consisting of the mud loach GH gene driven by the 2.3-kb lectin promoter exhibited significant growth stimulation. However, the extent of the growth acceleration in pmlectGH transgenics (six times maximum when assessed 2 months post hatching) was much less than that in transgenic individuals carrying the $pml{\beta}$-actGH construct. Additionally, the extraordinary gigantism that was common in $pml{\beta}$-actGH-transgenic mud loaches was diminished in transgenic loaches harboring the pmlectGH construct. Transgenic founders (pmlectGH) successfully transmitted their transgene into the next generation with up to 41% frequency. Growth stimulation also persisted in the transgenic F1 strains, with a seven-fold increase in maximum body weight at 6 months of age.

Variables Affecting Brand Relationship Quality of Fashion Product Consumers (패션상품 소비자들의 상표관계본질에 영향을 미치는 변인 연구)

  • Chae, Jin-Mie
    • Journal of the Korean Home Economics Association
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    • v.46 no.8
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    • pp.121-133
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    • 2008
  • The purpose of this study was to decipher the BRQ (Brand Relationship Quality) Construct of fashion product consumers and to identify influencing variables. Consumption values of consumers, fashion product attributes and marketing communication instruments were investigated as affecting variables. A questionnaire was distributed to 350 women between the age of 20 and 50 living in Seoul and other metropolitan areas from Mar. 5 to Mar. 18, 2008. Collected data were analyzed by factor analysis, reliability, multiple regression, frequency and percentage using SPSS (Version 15,0) statistical Package. The results were as follows: Firstly, the BRQ Construct of fashion product consumers was proved to be composed of five factors; 'self-connective love', 'pursuing symbol', 'trust', 'intimacy', and 'knowledge'. Secondly, consumption values had a significant effect on the BRQ Construct and their explanatory power was 32%, 'Epistemic value' was the most significant variable affecting BRQ. 'Functional value' had a significant influence on some BRQ factors. Thirdly, explanatory power of fashion product attributes affecting BRQ was 45%. 'Aesthetic attribute' had significant effect on all BRQ factors, while 'functional attribute' showed significant effect on only 'pursuing symbol', 'trust', and 'intimacy'. Finally, marketing communication mixes showed 21% explanatory power and 'word of mouth' and 'display' among marketing communication mixes were found as the most influential variables affecting the BRQ Construct.

Scalarization of HPF FORALL Construct (HPF FORALL 구조의 스칼라화(Scalarization))

  • Koo, Mi-Soon
    • Journal of the Korea Society of Computer and Information
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    • v.12 no.5
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    • pp.121-129
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    • 2007
  • Scalarization is a process that a parallel construct like an array statement of Fortran 90 or FORALL of HPF is converted into sequential loops that maintain the correct semantics. Most compilers of HPF, recognized as a standard data parallel language, convert a HPF program into a Fortran 77 program inserted message passing primitives. During scalariztion, a parallel construct FORALL should be translated into Fortran 77 DO loops maintaining the semantics of FORALL. In this paper, we propose a scalarization algorithm which converts a FORALL construct into a DO loop with improved performance. For this, we define and use a relation distance vector to keep necessary dependence informations. Then we evaluate execution times of the codes generated by our method and by PARADIGM compiler method for various array sizes.

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Active Network Performance Improvement for Security Application (보안 응용을 위한 능동 네트워크 성능 향상 방안)

  • 채철주;이명선;김상국;임정목;이성현;이재광
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2004.05b
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    • pp.416-419
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    • 2004
  • Computer simulation has used to a area of military training in recent years. Computer simulation endow a military nan with field raining such as combat experience without operating combat strength or capabilities. To samely construct simulation environment against actual combat environment is to well construct ㏈ to operate war game model, associate among federates on network. we construct virtual combat environment enabling to efficiently manage network traffic among federates(or active nodes) on active network that construct virtual military training spare such as urgent combat field needed to rapidly transfer combat information including image and video.

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A Study on Positioning of Nursing Service Image in General Hospital;Centering around 5 General Hospitals in Seoul (종합병원의 간호 서비스 이미지 포지셔닝 연구;서울시내 5개 병원을 중심으로)

  • Lim, Ji-Young
    • Journal of Korean Academy of Nursing Administration
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    • v.5 no.2
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    • pp.369-388
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    • 1999
  • This study is a descriptive research to establish a positioning strategy of nursing service image, making a perception map on each hospital's nursing service image and ranking construct factors of nursing service image. The results in this study were as follows; 1. The rank of construct factors of nursing service image which were thought important by subjects was the first 'Kindness', the second 'Reliability', the third 'Professional Knowledge', the fourth 'Responsibility', and the fifth 'Advanced Nursing Skill'. 2. The perception map for nursing service image on each hospital showed a hierarchial structure and positioned the first B hospital, the second C hospital, the third D hospital, the fourth A hospital, and the fifth E hospital. The perception map for construct factors of nursing service image and hospitals showed the first dimension : 'Reliability', the second dimension : Kindness', the third dimension : 'Mission', 'Accuracy', 'Neat Appearance', and 'Ethical Aspect', and the fourth dimension : 'Client Advocate', 'Humanistic Consideration', 'Professional Knowledge', 'Responsibility', and 'Advanced Nursing Skill'. 3. The findings of subject's characteristics which affected degree of likeness on each hospital nursing service image to make a marketing strategy were as follows : In A hospital D hospital, and E hospital, the subject group whose hospital dates were under 1 week, education level was upper graduate university, and age was between 40's and 50's, were appeared to have low nursing service image on following construct factors : 'Kindness', 'Reliability', 'Responsibility', 'Mission', 'Neat Appearance', and 'Humanistic Consideration'. In B hospital and C hospital the subject group whose hospital dates were under 1 week or over 5 weeks, sex was woman, and age was 20' s or 40's, having a admission experience on subject hospitals, were appeared to have low nursing service image on following construct factors : 'Professional Knowledge', 'Advanced Nursing Skill', 'Ethical Aspect', and 'Humanistic Consideration'. As the based on above results, nursing managers can use positioning method to develope a marketing strategy for nursing service image.

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Online Community Design: Review of Frameworks and Developing Online Community Construct

  • Oh Ki-Tae;Lee Kun-Pyo
    • Archives of design research
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    • v.19 no.3 s.65
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    • pp.131-142
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    • 2006
  • The purpose of this study is to develop an online community construct, which proposes an inclusive illustration of the structure of online communities, for online community designers. This study reviewed researches from psychology, sociology, management engineering, and practical reports to understand the characteristics and dynamics of online communities. The proposed online community construct visualizes the cognitive, affective, and behavioral aspects of online community. As the notion of community originates from geographical groups, and with the assumption that geographical community shares identical characteristics with online community, this study reviewed researches about geographical communities as a starting-point. Then the study went through three main perspectives (1) online community attributes, (2) sense of online community and (3) challenges of online community. Then this study proposed an online community construct that encompasses the reviewed frameworks. The online community can be seen as a congregation of members from two sources. One is from the 'Shared Goal' that meets the personal needs. Given the shared goal, members gather into the community without personal relationship and have more chances to feel the sense of belonging to their needs fulfillment or benefit. This befitting tendency leads to strengthening of membership. Public online forums fall under this classification. The other source is from the emotional connections that are already initiated by personal and casual contacts in the real world. The network of emotional connection can evolve into an online congregation of people under faint boundaries. Although there is no (or weak) shared goal, members are strongly bound to other members. Personal homepage or web log (blog) can be classified as an example of relationship-oriented community.

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The Effect of the Perception on the Physical Environment in Discount Stores on Customer Satisfaction and Intention (대형할인점의 물리적 환경에 대한 지각이 고객만족과 의도에 미치는 영향)

  • Koo, Yeoung-duk
    • Journal of Distribution Science
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    • v.3 no.2
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    • pp.29-55
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    • 2005
  • Since discount stores should be operated mostly by the non-human resources regarding the natural characteristics of the business, it is possibly said that their physical environments have been extremely significant. This study attempts to develop the measurement items and construct factors which are especially relevant to the physical environments of the discount stores and examine how the customer satisfaction and intention influence on these construct factors. These provide a useful framework for understanding the problems of measuring and evaluating the physical environments of discount stores. The final factor analysis for each construct factors resulted as four factors, and two categories(amenity and convenience) represented the characteristics of same construct such as the visibility. Then convenience is eliminated, and an attempt was made to add the new variable what is called the visibility. As a result, final construct factors that would be used in this study are made of four items; amenity, accessibility, POP and visibility. This study utilizes the LISREL 8.12 to evaluate the validity of factor structures and measurement model involving the physical environment in discount store. Finally, for the success of the projects, I think that it is necessary to provide comprehensive efforts continuously.

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A Comparative Study on the Impact Factors and Moderator of Incentives of Knowledge Transfer Process in Organizations -Focused on Nonprofit Organization and Profit Organization- (조직내 지식이전 프로세스의 영향요인과 인센티브의 조절효과에 관한 비교연구 - 비영리조직과 영리조직을 중심으로 -)

  • Kang, Ju-Seon;Ko, Yoon-Jung
    • Management & Information Systems Review
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    • v.32 no.2
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    • pp.259-287
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    • 2013
  • This study is to comparate on impact factors and moderator of incentives of knowledge transfer between Nonprofit and Profit organization. In nonprofit organization, the results founded that individual information capability, quality of knowledge, and quality of construct were statistically significant in knowledge acquisition, informal communication and quality of construct were statistically significant knowledge sharing, and individual information capability, informal communication, quality of knowledge and quality of construct were statistically significant knowledge use. Also interactive effect of knowledge sharing and Psychological Physical incentive was statistically significant knowledge use. In profit organization, the results founded that individual information capability, informal communication, quality of knowledge, and quality of construct were statistically significant in knowledge acquisition, quality of knowledge and quality of construct were statistically significant knowledge sharing, and individual information capability, informal communication, and formal communication were statistically significant knowledge use. Also interactive effect of knowledge acquisition and Psychological Physical incentive was statistically significant knowledge use.

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The Measurement Development of Korean Families with Adolescents' Family Resilience: Focusing on the Evaluation of the Measurements' Construct Validity (청소년자녀기 가족의 레질리언스(Resilience) 척도개발 연구: 척도의 타당화 검증을 중심으로)

  • Ok, Sun-Wha;Rueter, Martha;Lee, Mi-Sook;Lee, Ju-Lie;Kwon, Hee-Kyung;Nam, Young-Joo
    • Journal of the Korean Home Economics Association
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    • v.44 no.1 s.215
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    • pp.59-74
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    • 2006
  • As an exploratory inquiry prior to developing a measure to assess Korean families with adolescents' family resilience, this study examined the construct validity of the family resilience measures that were developed in the U. S. Iowa Youth and Families Project(Conger & Elder, 1994): emotional support between spouses, problem solving style between spouses, and parenting behavior. The participants were 277 pairs of middle aged, middle class husbands and wives (554 individuals) who were the parents of first graders in middle schools in the metropolitan areas around Seoul. The results of exploratory and confirmatory factor analysis supported the construct validity, indicating that emotional support, problem solving style, and parenting behavior may be used as measures to assess Korean family resilience. However, further tests of convergent validity using various assessment methods and analytical procedures are needed to ensure the construct validity of family resilience measures.

Keratinocyte Migration in a Three-Dimensional In Vitro Wound Healing Model Co-Cultured with Fibroblasts

  • Iyer, Kritika;Chen, Zhuo;Ganapa, Teja;Wu, Benjamin M.;Tawil, Bill;Linsley, Chase S.
    • Tissue Engineering and Regenerative Medicine
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    • v.15 no.6
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    • pp.721-733
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    • 2018
  • BACKGROUND: Because three-dimensional (3D) models more closely mimic native tissues, one of the goals of 3D in vitro tissue models is to aid in the development and toxicity screening of new drug therapies. In this study, a 3D skin wound healing model comprising of a collagen type I construct with fibrin-filled defects was developed. METHODS: Optical imaging was used to measure keratinocyte migration in the presence of fibroblasts over 7 days onto the fibrin-filled defects. Additionally, cell viability and growth of fibroblasts and keratinocytes was measured using the $alamarBlue^{(R)}$ assay and changes in the mechanical stiffness of the 3D construct was monitored using compressive indentation testing. RESULTS: Keratinocyte migration rate was significantly increased in the presence of fibroblasts with the cells reaching the center of the defect as early as day 3 in the co-culture constructs compared to day 7 for the control keratinocyte monoculture constructs. Additionally, constructs with the greatest rate of keratinocyte migration had reduced cell growth. When fibroblasts were cultured alone in the wound healing construct, there was a 1.3 to 3.4-fold increase in cell growth and a 1.2 to 1.4-fold increase in cell growth for keratinocyte monocultures. However, co-culture constructs exhibited no significant growth over 7 days. Finally, mechanical testing showed that fibroblasts and keratinocytes had varying effects on matrix stiffness with fibroblasts degrading the constructs while keratinocytes increased the construct's stiffness. CONCLUSION: This 3D in vitro wound healing model is a step towards developing a mimetic construct that recapitulates the complex microenvironment of healing wounds and could aid in the early studies of novel therapeutics that promote migration and proliferation of epithelial cells.