• Entomological Research
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• v.48 no.6
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• pp.533-539
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• 2018

The RNA interference (RNAi) has been considered as an important genetic tool and applied to develop a new living modified (LM) crop trait which is an improvement of nutrient quality or pest management. The RNAi of DvSnf7 has been used for resistance to LM maize and the Western Corn Rootworm which is a major agricultural pest for the US Corn Belt. Most of the environmental risk assessments (ERA) of double strand RNA (dsRNA) have been performed using in vitro transcript products, and not in vivo expressed product. A large amount of dsRNA was required for the acute toxicity assay of water fleas. Therefore development of massive dsRNA purification techniques is critical. Daphnia, a freshwater microcrustacean, is a model organism for studying cellular and molecular mechanism involved in life history traits and ecotoxicology. In this study, we established the massive dsRNA purification method using Escherichia coli and implemented acute toxicity assays to Daphnia magna. As a result, the present RNase A and DNase I, dsRNA was efficiently purified without any special techniques or equipment. Even though purified dsRNA existed during the acute toxicity test, lethality or abnormal behavior were not observed in D. magna. These results indicated that GFP and DvSnf7 dsRNA were not significantly affected to D. magna due to their lack of sequence matching in its genome. The purification method of dsRNA and the acute toxicity assay of water fleas using purified dsRNA would be suitable for the toxicological studies of LMOs to aquatic non-target organisms.

• Journal of the Economic Geographical Society of Korea
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• v.7 no.2
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• pp.157-170
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• 2004

The Boundary Region in Gyeonggi Province has environmental vulnerability due to the increasing development pressure although usually regarded as an ecologically well-preserved region. This paper is aimed to analyze the status of environmental loading on natural resources of the Boundary Region in Gyeonggi Province through investigating land-use conversion pattern during the past 15 years(1985-2000), the attitude of the local community towards conservation policy, and the status of environmental treatment facilities for water quality management. The results are as follows. Firstly, the development pressure has been focused upon the southwestern part of the Boundary Region in Gyeonggi Province such as Gimpo, Paju, Dongduocheon, and Yangju since 1995. Secondly, local residents' strong aversion exists towards the conservation policy led by the government because of their underdevelopment. Finally, the risk of environmental degradation is high because the region is not equipped with adequate environmental treatment facilities for water quality management. The status of environmental loading in the Boundary Region suggests the necessity to establish a well-designed management plan for natural resources in this area.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.13 no.6
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• pp.117-131
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• 2010

This study was conducted to utilize as basic information for the construction of conservation and estimation system for Palustrine wetland, which was badly managed and imprudently reclaimed, through the analysis of distribution characteristics and the estimation of conservation value for sample sites (eight wetlands) in rural area. As the result of wetland type classification, these wetlands was classified by 4 types (Permanent freshwater marshes/pools, ponds, Aquaculture ponds, and Seasonally flooded agricultural land) by Ramsar system, 3 types (Emergent Wetland, Aquatic Bed, and Scrub-Shrub Wetland) by NWI (Cowardin) System, 5 types (Farm Pond Depression, Under-flow wetland, Man-made Pond Depression, Abandoned Paddy Fields Wetland, and Reservoir Shore) by National Wetland's Categorical System, and 3 types (Aquatic Bed Wetland, Emergent Wetland, and Forested Wetland) by Lee (2000) System. These results suggest us developing the new type classification system for small Palustrine wetland in Korean rural areas. The score of function assessment (The Modified RAM) for small Palustrine wetlands was high at the wetlands nearby hills and rice paddy fields, and low at those nearby upper fields, which was mainly affected by land-use and vegetation. The functions as 'Flood/Storm Water Storage', 'Runoff Attenuation', 'Water Quality Protection' were resulted by the structural difference of inflow and outlet. Some functions as 'Wetland size', 'Wetland to immediate watershed ratio', 'Presence of boat traffic', 'Maximum water depth', 'Fetch of water's body' of RAM were not appropriate in evaluation of small wetlands in rural area. Which suggest us developing the new function assessment system for small Palustirne wetland in Korean rural areas.

• Journal of Wetlands Research
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• v.11 no.1
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• pp.15-27
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• 2009

To reveal conservation value of Sowhangbyungsan-neup among wetlands in Mt. Odae designated as a Ramsar convention site in 2008, ecological characteristics were investigated. The maximum depth of sediment was 86cm and average at central part was 56cm. Average pH of water was 5.8 and this indicates that this wetland has the characteristics between bog and fen. Average electron conductivity was $11{\mu}S$/cm, which is the lowest among Korean wetlands. Nutrient status was oligotrophic based on contents of cation, nitrate, ammonia, soluble reactive phosphorus in water. Out-flow of water was related with the 3 day cumulative precipitation. Soil texture was loam and nutrient level was very low. Vascular plants of 45 family, 95 genus, 121 species, 2 subspecies, 16 variety, 4 forma, total 121 taxa were recorded. Main plant communities on Sphagnum base were Osmunda cinnamomea community, Carex dispalata community and Carex curta community. The results indicated that this wetland has very different ecological characteristics than others in Korea and deserves conservation value well. Also, this study revealed that there is no evident threatening factor and this wetland will have characteristics of fen continuously in near future.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.14 no.1
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• pp.231-243
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• 1994

A stochastic programming model for river water quality management was developed. River water quality, river flow, quality and flowrate of the wastewater treatment plant inflow were treated as random variables in the model. Withdrawal for water supply and submerged weir reaeration were included in the model itself. A probabilistic model was formulated to compute the expectation and variance of water quality using Streeter-Phelps equation. Chance constraints of the optimization problem were converted to deterministic equivalents by chance constrained method. Objective function was total annual treatment cost of all wastewater treatment plants in the region. Construction cost function and O & M cost function were derived in the form of nonlinear equations that are functions of treatment efficiency and capacity of treatment plant. The optimization problem was solved by nonlinear programming. This model was applied to the lower Han River. The results show that the reliability to meet the DO standards of the year 1996 is about 50% when the treatment level of four wastewater treatment plants in Seoul is secondary treatment, and BOD load from the tributary inflows is the same as present time. And when BOD load from Tanchon, Jungrangchon, and Anyangchon is decreased to 50%, the reliability to meet the DO standards of the year 1996 is above 60%. This results indicated that for the sake of the water quality conservation of the lower Han River, water quality of the tributaries must be improved, and at least secondary level of treatment is required in the wastewater treatment plants.

• Applied Chemistry for Engineering
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• v.21 no.6
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• pp.632-637
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• 2010

Considering the water quality regulation of total emission system which is enforced with hydro TMS and so on operating currently, automatic water sampler must be necessary in the viewpoint of national interest for conservation of water resources. This study aimed to develope automatic water sampler for the purpose of decreasing spends on foreign currency which is relied on import from abroad and upgrading the functional efficiency simultaneously. We have made an effort for developing results as follows with some cases. First, flow meter with a sensor equiped into automatic water sampler for field measurement which can perform linkage operation was developed. Second, remote-D/L system was developed which was able to monitor and store some transmitted data from practical measurement sensor. Also, automatic water sampler was developed in this research that operates in a sewage treatment plant, and finally, we confirmed that our system is able to apply to the field well.

• The Journal of the Convergence on Culture Technology
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• v.8 no.5
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• pp.677-684
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• 2022

This study is a basic study to evaluate the ecological environment of Changwon City. The study site was 6 ecotourism districts in Changwon-si, and the ecotourism index was evaluated through direct visits and interviews with the person in charge through preliminary research and various literature data from June to July 2021. There are six ecotourism indicators: climate crisis, air quality improvement, water conservation, natural coexistence, citizen participation, and tourism resource management. When looking at the scores for the six ecotourism areas in Changwon, it received the highest score in the areas of natural coexistence and air quality improvement. However, the ecoregion received the lowest score in the water resource conservation category, indicating that the water resource conservation as a whole was weak. Next, tourism resource management, climate crisis, and citizen participation are at the same level in all regions. As a result of the evaluation through the low-carbon ecotourism index, the Changwon City low-carbon ecotourism certification system should first be operated as a measure to revitalize the ecotourism region. It is necessary to prepare a low-carbon ecotourism level for Changwon City. Second, it is very important to guide and promote ecotourism areas to revitalize ecotourism areas. Lastly, to operate a sustainable eco-environment area, it is necessary to operate using local governance above all else.

• Journal of Conservation Science
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• v.24
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• pp.37-42
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• 2008

Stability of the moving historic sites have something to do with the degree of easiness of work as well as physical property of polymer product. These agents should be able to use without the effect of outer environments like water or low temperature and must have stability during progress of working the peel off Urethane pre-product from Epoxy surface. So, we synthesized low viscosity epoxy resin and hardener with best physical quality and separation media for the moving of historical sites. These products have very good tension strength, adhesion strength, low viscosity and various physical properties that the users want. Particularly, separation media products have good separation of Urethane pre-products surface and Epoxy final product surface.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.27 no.4
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• pp.1-14
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• 2024

Otters, designated as Class I endangered wildlife due to population declines resulting from urban development and stream burial, have seen increased appearances in freshwater environments since the nationwide ban on stream filling in 2020 and the implementation of urban stream restoration projects. There is a pressing need for scientific and strategic conservation measures for otters, an umbrella and vulnerable species in aquatic ecosystems. Therefore, this study predicts potential otter habitats using the species distribution model MaxEnt, focusing on Hwangguji Stream in Suwon, and proposes conservation strategies. Otter signs were surveyed over three years from 2019 to 2021 with citizen scientists, serving as presence data for the model. The model's outcomes were enhanced by analyzing 'river nature map' as a boundary. MaxEnt compared the performance of 60 combinations of feature classes and regularization multipliers to prevent model complexity and overfitting. Additionally, unmanned sensor cameras observed otter density for model validation, confirming correlations with the species distribution model results. The 'LQ-5.0' parameter combination showed the highest explanatory power with an AUC of 0.853. The model indicated that the 'adjacent land use' variable accounted for 31.5% of the explanation, with a preference for areas around cultivated lands. Otters were found to prefer shelter rates of 10-30% in riparian forests within 2 km of bridges. Higher otter densities observed by unmanned sensors correlated with increasing model values. Based on these results, the study suggests three conservation strategies: establishing stable buffer zones to enhance ecological connectivity, improving water quality against non-point source pollution, and raising public awareness. The study provides a scientific basis for potential otter habitat management, effective conservation through governance linking local governments, sustainable biodiversity goals, and civil organizations.

• Journal of the Korean Institute of Rural Architecture
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• v.21 no.3
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• pp.69-75
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• 2019

The purpose of this study is to provide the conservation management direction about cultural landscape of the traditional villages in the area of Huizhou through the importance-performance analysis. The results of the survey on the importance - performance of 261 people who had many visits to various cultural landscapes of Huizhou villages such as Hongchun, Sidichun etc. are as follows. It was appeared that the item of 'good air' is the highest level of importance and satisfaction and is best condition. The item of 'historical conservation' is the high level of importance, but the level of satisfaction is low, so continuous improvement is necessary. The item of 'Old garden conservation' has high satisfaction but low importance, so it needs to improve its importance through subsequent improvement. The item of 'Keeping of old cemetary and old tower' has low importance and satisfaction but it is above average, so continuous maintenance is needed. The items of 'Preservation of stone gate' and 'Preservation of famous man's birth house' all have slightly higher satisfaction and lower importance. Both items are highly satisfied with their importance, so they should avoid excessive efforts and maintain the present status. Satisfaction with the item of 'water environment and water quality' was the lowest, and the problem of the present condition is the most serious, and a major improvement is needed. The importance and satisfaction of 'Commercial development of old villages' was the lowest among all items. This item is causing serious problems and should provide an overall improvement way to drastically improve the importance and satisfaction. Concentration is necessary to improve the environment of the whole villages, to protect the ecological plants and animals in old villages, to transfer and improve the culture of old villages, to maintain sustainability of the villages, and to protect old temple and old rock in old villages. As the level of satisfaction with sustainability is the lowest in the part of 'Concentrate here', there is a great concern about sustainability. Therefore, it is necessary to focus on sustainability and concentrate on sustainable development.