• Tribology and Lubricants
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• v.34 no.6
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• pp.325-331
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• 2018

Automobiles and systems requiring high gear ratios and high power densities generally use worm gears. In particular, as worm gears have a small volume and self-locking function, home appliances such as refrigerators and washers consist of worm gears. We can classify worm gears into cylindrical worms and rectangular worms. According to the AGMA standard, there are four types of cylindrical worms, ZA, ZN, ZK and ZI, depending on the machining of the worm shaft. It is preferable to use a ZI-type worm shaft, which is a combination of a worm wheel having an involute helical tooth surface and a conjugate tooth surface. However, in many cases, industries mostly use ZK, ZN, and ZA worm shafts because of the ease of processing. This paper presents numerical approaches to produce ZI and ZA worm surfaces and worm wheel. For the analysis of the transmission error of a worm gear system, this study (1) generates surface profile functions of ZI profile worm gear and worm shaft based on the common rack theory, (2) adopts the Newton-Raphson method for the analysis of the gear surface contact condition, and (3) presents and compares the corresponding transmission errors of ZI and ZA worm gears.

• Mass Spectrometry Letters
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• v.9 no.4
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• pp.91-94
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• 2018

Bilin tetrapyrroles are metabolic products of the breakdown of porphyrins within a species. In the case of mammals, these bilins are formed by the catabolism of heme and can be utilized as either biomarkers in disease or as an indicator of human waste contamination. Although a small subset of bilin tandem mass spectrometry reports exist, limited data is available in online databases for their fragmentation. The use of fragmentation data is important for metabolomics analyses to determine the identity of compounds detected within a sample. Therefore, in this study, the fragmentation of bilins generated by positive ion mode electrospray ionization is examined by collision-induced dissociation (CID) as a function of collision energy on an FT-ICR MS. The use of the FT-ICR MS allows for high mass accuracy measurements, and thus the formulas of resultant product ions can be ascertained. Based on our observations, fragmentation behavior for hemin, biliverdin and its dimethyl ester, phycocyanobilin, bilirubin, bilirubin conjugate, mesobilirubin, urobilin, and stercobilin are discussed in the context of the molecular structure and collision energy. This report provides insight into the identification of structures within this class of molecules for untargeted analyses.

• Nuclear Engineering and Technology
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• v.56 no.1
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• pp.265-274
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• 2024

The current research focuses on the development of a numerical approach to forecast strongly subcooled flow boiling of FC-72 as the refrigerant in various vertical minichannel shapes for high-heat-flux cooling applications. The simulations are carried out using the Volume of Fluid method with the Lee phase change model, which revealed some inherent flaws in multiphase flows that are primarily due to an insufficient interpretation of shearlift force on bubbles and conjugate heat transfer against the walls. A user-defined function (UDF) is used to provide specific information about this noticeable effect. The influence of shape and the inlet mass fluxes on the flow patterns, heat transfer, and pressure drop characteristics are discussed. The computational results are validated with experimental measurements, where excellent agreements are found that prove the efficiency of the present numerical model. The findings demonstrate that the heat transfer coefficient decreases as the mass flux increases and that the constriction design improves the thermal performance by 24.68% and 10.45% compared to the straight and expansion shapes, respectively. The periodic constriction sections ensure good mixing between the core and near-wall layers. In addition, a slight pressure drop penalty versus the thermal transfer benefits for the two configurations proposed is reported.

• Journal of Biomedical Engineering Research
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• v.42 no.3
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• pp.125-142
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• 2021

The POCT (point-of-care test) sensing that has been a fast-developing field is expected to be a next generation technology in health care. The POCT sensors for the detection of proteins, small molecules and especially nucleic acids have lately attracted considerable attention. According to the World Health Organization (WHO), the POCT methods are required to follow the ASSURED guidelines (Affordable, Sensitive, Specific, User- friendly, Robust and rapid, Equipment-free, Deliverable to all people who need the test). Recently, several CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) based diagnostic techniques using the sensitive gene recognition function of CRISPR have been reported. CRISPR/Cas (Cas, CRISPR associated protein) systems based detection technology is the most innovative gene analysis technology that is following the ASSURED guidelines. It is being re-emerged as a powerful diagnostic tool that can detect nucleic acids due to its characteristics that enable rapid, sensitive and specific analyses of nucleic acid. The first CRISPR-based diagnosis begins with the discovery of the additional function of Cas13a. The enzymatic cleavage occurs when the conjugate of Cas protein and CRISPR RNA (crRNA) detect a specific complementary sequence of the target sequence. Enzymatic cleavage occurs on not only the target sequence, but also all surrounding non-target single-stranded RNAs. This discovery was immediately utilized as a biosensor, and numerous sensor studies using CRISPR have been reported since then. In this review, the concept of CRISPR, the characteristics of the Cas protein required for CRISPR diagnosis, the current research trends of CRISPR diagnostic technology, and some aspects to be improved in the future are covered.

• Korean Journal of Food Science and Technology
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• v.35 no.3
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• pp.366-372
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• 2003

Enzyme-linked immunosorbent assay (ELISA) for assaying the 5-enolpyruvyshikimate-3-phosphate synthase from Agribacterium sp. CP4 (CP4 EPSPS) in genetically modified soybeans was developed. Polyclonal and monoclonal antibodies (Pab, Mab) specific to the CP4 EPSPS were produced. When using the Pab, the detection limit of sandwich ELISA toward CP4 EPSPS (0.03 ${\mu}g/mL$) was better than that of competitive indirect ELISA(ciELISA) (1 ${\mu}g/mL$). It was found that 2 of 3 monoclonal antibodies, Mab1 and Mab2, recognized the same antigenic determinant on CP4 EPSPS, but Mab3 recognized different antigenic determinant when competitive ELISA was performed using the Mabs. On the other hand, when the sensitivity of sandwich ELISA using combination of Pab and/or Mabs was determined, the sandiwich ELISA using Mab2 as a capture antibody and Pab-HRP as a secondary antibody showed the lowest detection limit of CP4 EPSPS (0.02 ${\mu}g/mL$). The sandwich ELISA developed in this study could be applied to detect glyphosate-tolerant soybeans.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.38 no.7
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• pp.639-646
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• 2014

The comparative analysis of near-wall treatment methods that affect the prediction of heat transfer over the gas turbine nozzle guide vane were presented. To achieve this objective, wall-function and low Reynolds number methods, and the transition model were applied and simulated using NASA's C3X turbine vane. The predicted turbine vane surface pressure distribution data using the near-wall treatment methods were found to be in close agreement with experimental data. However, the predicted vane metal temperature and heat transfer coefficient displayed significant differences. Overall, the low Reynolds method and transition model did not offer specific advantages in the prediction of temperature and heat transfer than did the wall-function method. The Reynolds stress model used along with the wall-function method resulted in a relatively high accuracy of prediction of the vane metal temperature and heat transfer coefficient.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.2
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• pp.608-616
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• 2018

If a general nonlinear system is linearized by the successive multiplication of the 1st and 2nd order systems, then there are four types of poles in this linearized system: the pole of the 1st order system and the equal poles, two distinct real poles, and complex conjugate pair of poles of the 2nd order system. Linear Quadratic (LQ) control is a method of designing a control law that minimizes the quadratic performance index. It has the advantage of ensuring the stability of the system and the pole placement of the root of the system by weighted matrix adjustment. LQ control by the weighted matrix can move the position of the pole of the system arbitrarily, but it is difficult to set the weighting matrix by the trial and error method. This problem can be solved using the characteristic equations of the Hamiltonian system, and if the control weighting matrix is a symmetric matrix of constants, it is possible to move several poles of the system to the desired closed loop poles by applying the control law repeatedly. The paper presents a method of calculating the state weighting matrix and the control law for moving the equal poles with Jordan blocks to two real poles using the characteristic equation of the Hamiltonian system. We express this characteristic equation with a state weighting matrix by means of a trigonometric function, and we derive the relation function (${\rho},\;{\theta}$) between the equal poles and the state weighting matrix under the condition that the two real poles are the roots of the characteristic equation. Then, we obtain the moving-range of the two real poles under the condition that the state weighting matrix becomes a positive semi-finite matrix. We calculate the state weighting matrix and the control law by substituting the two real roots selected in the moving-range into the relational function. As an example, we apply the proposed method to a simple example 3rd order system.

• Journal of the Korean Society for Precision Engineering
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• v.24 no.3 s.192
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• pp.100-107
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• 2007

A internal lobe pump is suitable for oil hydraulics of machine tools, automotive engines, compressors, constructions and other various applications. In particular, the pump is an essential machine element of an automotive engine to feed lubricant oil. The subject of this paper is the theoretical analysis of internal lobe pump whose the main components are the rotors: usually the outer one is characterized by lobes with circular shape, while the inner rotor profile is determined as conjugate to the other. The topic of this paper is the design of a new rotor, which is based on specific performance as different types depending on the shape of the lobe of the outer rotor. First, the design of internal lobe pumps with circular, elliptical, and their combined lobe profiles is considered. The latter is a new type of lobe profile with special shape whose curvature follows a definite function. Then we introduce the performance indexes used for the comparison. Some of these indexes, such as flow rate and flow rate irregularity, are commonly used for the comparison, while specific slipping is particularly suitable in this case. It is possible to notice that the circular and elliptical type is comparable to the circular one or the elliptical one in terms of flow rate irregularity, but has improved performance in terms of specific slipping. Results obtained from the analysis enable the designer and manufacturer of oil pump to be more efficient in this field.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.8 no.5
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• pp.1134-1140
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• 2004

In this paper, we investigated the effect of cross phase modulation(XPM) on compensation for WDM channel distortion due to chromatic dispersion, self phase modulation and XPM as a function of fiber dispersion coefficient and modulation format in 320 Gbps WDM systems. The considered WDM transmission system is based on mid-span spectral inversion(MSSI) compensation method, which has highly nonlinear dispersion shifted fiber(HNL-DSF) as nonlinear medium of optical phase conjugator (OPC) in the mid-way of total transmission line. We confirmed that the maximum channel input power resulting 1 dB eye opening penalty is reduced due to XPM effect on channel distortion, even if MSSI method was applied to WDM system. But, we confirmed that the effect of XPM on channel distortion becomes decrease as fiber dispersion coefficient of WDM system becomes larger. Futhermore, we confirmed that NRZ is better than RZ as a modulation format for similarly compensating overall WDM channels in WDM system with large fiber dispersion coefficient in order to minimize the effect of the XPM on channel distortion.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.2
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• pp.155-161
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• 2009

Mammal microsomal glutathione transferase 1 (MGST1) can conjugate many toxic or carcinogenic substances and depress oxidative stress. In this study, Chicken MGST1 and its variants were cloned for the first time and were composed of 956 or 944 nucleotides. The 12 nt deletion in the exon 2 did not alter the GT-AG rule and the ORFs for the two MGST1 variants were the same, which both comprised 465 nucletides and encoded a peptide with 155 amino acids. It was found that the two different splice variants identified using RT-PCR expressed in all three organs investigated of Dwarf Brown Chicken, namely liver, spleen and shell gland. Moreover, the expression level of MGST1 mRNA in the liver of Dwarf Brown chickens was the highest (p<0.01), and there were no significant differences between the spleen and the shell gland. These results provide a base for studying the biological function of Chicken MGST1.