• Title/Summary/Keyword: concentration image

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Genotoxicity of Taxol and 10-Deacetyl Baccatin III Using Single Cell Gel Electrophoresis (Comet Assay) in Chinese Hamster Lung Fibroblast

  • Kim, Hyun-Joo;Kim, Kyung-Ran;Youn, Ji-Youn;Kim, Min-Hee;Ryu, Jae-Chun
    • Proceedings of the Korea Society of Environmental Toocicology Conference
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    • 1996.12a
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    • pp.61-61
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    • 1996
  • Taxol is used as cancer therapeutic agent. It has been known as weak posotive of chromosome aberration assay in vitro in our previous results (Ryu et al., 1996) and potent clastogens in the mouse bone marrow micronucleus (Tinwell and Ashby, 1994). We performed microgel electrophoresis to determine the effect of taxol and it's precursor 10-deacetyl baccatin III(DAB) on DNA. Microgel electrophoresis is useful, rapid, simple, visual, and sensitive technique for measuring DNA breakage and repair mechanisms in mammalian 근ells. The range of concentration used for taxol were 854, 427, 213.5, 106.8, 53.4 Ug/ml, for DAB 910 ,455, 227.5 U9/ml, Cell viability always exceed 85%. We analyzed the results by using the special software of image analyzer for this comet assay (Komet 3.0). By using this image analyzer software , we can get the result as the tail moment ((mean of tail length - mean of head lengh) x tail%DNA/100). A slight increase in DNA migration was observed for taxol at the concentration of 854 Ug/m4 in the absence of S9 mixture. No increased DNA migration was observed after treatment with DAB.

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HYPERSPECTRAL IMAGERY AND SPECTROSCOPY FOR MAPPING DISTRIBUTION OF HEAVY METALS ALONG STREAMLINES

  • Choe, Eun-Young;Kim, Kyoung-Woong;Meer, Freek Van Der;Ruitenbeek, Frank Van;Werff, Harald Van Der;Smeth, Boudewijn De
    • Proceedings of the KSRS Conference
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    • 2007.10a
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    • pp.397-400
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    • 2007
  • For mapping the distribution of heavy metals in the mining area, field spectroscopy and hyperspectral remote sensing were used in this study. Although heavy metals are spectrally featureless from the visible to the short wave infrared range, possible variations in spectral signal due to heavy metals bound onto minerals can be explained with the metal binding reaction onto the mineral surface. Variations in the spectral absorption shapes of lattice OH and oxygen on the mineral surface due to the combination of heavy metals were surveyed over the range from 420 to 2400 nm. Spectral parameters such as peak ratio and peak area were derived and statistically linked to metal concentration levels in the streambed samples collected from the dry stream channels. The spatial relationships between spectral parameters and concentrations of heavy metals were yielded as well. Based on the observation at a ground level for the relationship between spectral signal and metal concentration levels, the spectral parameters were classified in a hyperspectral image and the spatial distribution patterns of classified pixels were compared with the product of analysis at the ground level. The degree of similarity between ground dataset and image dataset was statistically validated. These techniques are expected to support assessment of dispersion of heavy metal contamination and decision on optimal sampling point.

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Design of New Fine Dust Measurement Method applying LoG Edge Detection Technique (LoG 윤곽선 검출 기법을 적용한 새로운 미세먼지 측정 방법 설계)

  • Jang, Taek-Jin;Lin, Chi-Ho
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.22 no.5
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    • pp.69-73
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    • 2022
  • In this paper, we propose a new method for measuring fine dust through a LoG(Laplacian of Gaussian)-based edge detection technique. CCTV-based images in a video are collected for fine dust measurement, and image ranges are designated through RoI(Region of Interest). After clustering by applying the GMM(Gaussian Mix Model) to the specified area, we detect edge through the LoG algorithm and measure the detected edge strength. The concentration of fine dust is determined based on the measured intensity data of the edge. In this paper, we propose algorithm as the effectiveness of experiment. As a result of collecting and applying CCTV image in the video installed around the laboratory of this school for a month from June to July, the measured result value was proved through this experiment to be sufficient to calculate the concentration and range of fine dust.

Learning efficiency checking system by measuring human motion detection (사람의 움직임 감지를 측정한 학습 능률 확인 시스템)

  • Kim, Sukhyun;Lee, Jinsung;Yu, Eunsang;Park, Seon-u;Kim, Eung-Tae
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • fall
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    • pp.290-293
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    • 2021
  • In this paper, we implement a learning efficiency verification system to inspire learning motivation and help improve concentration by detecting the situation of the user studying. To this aim, data on learning attitude and concentration are measured by extracting the movement of the user's face or body through a real-time camera. The Jetson board was used to implement the real-time embedded system, and a convolutional neural network (CNN) was implemented for image recognition. After detecting the feature part of the object using a CNN, motion detection is performed. The captured image is shown in a GUI written in PYQT5, and data is collected by sending push messages when each of the actions is obstructed. In addition, each function can be executed on the main screen made with the GUI, and functions such as a statistical graph that calculates the collected data, To do list, and white noise are performed. Through learning efficiency checking system, various functions including data collection and analysis of targets were provided to users.

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Photodynamic therapy with chlorin e6-induced cervical cancer cell death (Chlorin e6의 농도별 Photodynamic therapy을 통한 자궁경부암 세포의 사멸도 측정)

  • Lee, Eonjin;Choe, Se-woon
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2022.10a
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    • pp.323-325
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    • 2022
  • In this paper, after administration of Chlorin e6 to kill cervical cancer cells, PDT (photodynamic therapy) using laser or LED was studied for Ce6 concentration and apoptosis by administration time. After photostimulation was applied to cervical cancer cells, qualitative and quantitative evaluation through imaging and quantitative evaluation using CCK-8 were performed. As a result of the experiment, as the concentration of Ce6 increased, a large amount of cells were killed, and it was confirmed that the Ce6+PDT test group killed more. When comparing the LED and the laser, the laser was able to kill cells only at a local location, and the LED showed the result that it was possible to kill the cells in a wide range. In addition, when irradiating a local location, such as a laser, when measuring the cell viability, it is judged to be accurate to image processing the location.

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Suppression of Moiré Fringes Using Hollow Glass Microspheres for LED Screen (중공 미소 유리구를 이용한 LED 스크린 모아레 억제)

  • Songeun Hong;Jeongpil Na;Mose Jung;Gieun Kim;Jongwoon Park
    • Journal of the Semiconductor & Display Technology
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    • v.22 no.3
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    • pp.28-35
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    • 2023
  • Moiré patterns emerge due to the interference between the non-emission area of the LED screen and the grid line in an image sensor of a video recording device when taking a video in the presence of the LED screen. To reduce the moiré intensity, we have fabricated an anti-moiré filter using hollow glass microspheres (HGMs) by slot-die coating. The LED screen has a large non-emission area because of a large pitch (distance between LED chips), causing more severe moiré phenomenon, compared with a display panel having a very narrow black matrix (BM). It is shown that HGMs diffuse light in such a way that the periodicity of the screen is broken and thus the moiré intensity weakens. To quantitatively analyze its moiré suppression capability, we have calculated the spatial frequencies of the moiré fringes using fast Fourier transform. It is addressed that the moiré phenomenon is suppressed and thus the amplitude of each discrete spatial frequency term is reduced as the HGM concentration is increased. Using the filter with the HGM concentration of 9 wt%, the moiré fringes appeared depending sensitively on the distance between the LED screen and the camera are almost completely removed and the visibility of a nature image is enhanced at a sacrifice of luminance.

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Evaluation of the X-ray Shielding Ability of Lead Free Board Shielding in the CT Room (CT실에서 무연보드 차폐체의 X선 차폐능력 평가)

  • Sung-Joon Kim;Tae-Ho Han;Hyo-Won Lee;Yu-Whan Oh;Seung-Chul Kim;Jung-Min Kim
    • Journal of radiological science and technology
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    • v.47 no.4
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    • pp.249-254
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    • 2024
  • This study compared the X-ray shielding abilities of the shields using Computed Radiography(CR) System after manufacturing a lead-free boards using gypsum and BaSO4, an eco-friendly X-ray shielding material. Total six lead-free boards were manufactured with BaSO4 concentrations of 25 %, 50 % and thickness of 10 mm, 15 mm, 20 mm respectively, and additional thickness of 1.0 mm, 1.5 mm, 2.0 mm leads were prepared. In the experiment, Nine shields were placed on the Image Plate and placed in a Computed Tomography(CT) Room where CT scans were performed for 2 weeks. After that, the X-ray image of the shields were obtained through CR Reader, and Pixel Value(PV) were measured to evaluated the X-ray shielding abilities of the lead-free shields. The criterion for evaluating the shields was determined by comparing PV of lead-free board to that of the 1.5 mm thickness lead used in the CT rooms. As a result of the experiment, the PV of the lead-free boards within 25 % of the BaSO4 concentration and within 10 mm of the thickness were not enough to be used as X-ray shields in the CT Room because they did not reach the PV of the 1.5 mm thickness lead. BaSO4 concentration of 50 % at 20 mm thickness showed PV of 1.5 mm lead thickness or more indicating that it has an X-ray shielding ability to replace lead in the CT room

The Comparison of Water Quality of Daecheong-Dam basin According to the Data Sources of Land Cover Map (토지피복도 자료원에 따른 대청댐유역 수질특성 비교)

  • Lee, Geun Sang;Park, Jin Hyeog;Choi, Yun Woong
    • Spatial Information Research
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    • v.20 no.5
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    • pp.25-35
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    • 2012
  • This study compared the influence of water quality according to the data sources of spatial information. Firstly, land cover map was constructed through image classification of Daecheong-dam basin and the accuracy of image classification from satellite image showed high as 88.76% in comparison with the large-scaled land cover map in Ministry of Environment, to calculate Event Mean Concentration (EMC) by land cover that impact on the evaluation of nonpoint source pollutant loads. Also curve number and direct runoff were calculated by spatial overlay with soil map and land cover map from image classification. And Seokcheon and Daecheong-Dam basin showed high in the analysis of curve number and direct runoff. Samgacheon-Joint and Sokcheon-Downstream basin showed high in the nonpoint source pollutant loads of BOD from direct runoff and EMC. And Samgacheon-Joint and Bonghwangcheon- Downstream basin showed high in the nonpoint source pollutant loads of TN and TP. Nonpoint source pollutant loads from image classification were compared with those by the land cover map from Ministry of Environment to present the effectivity of nonpoint source pollutant loads from satellite image. And Daecheong-Dam Upstream basin showed high as 10.64%, 11.70% and 20.00% respectively in the errors of nonpoint source pollutant loads of BOD, TN, and TP. Therefore, it is desirable that spatial information including with paddy and dry field is applied to the evaluation of nonpoint source pollutant loads in order to simulate water quality of basin effectively.

Estimation of Nitrate Nitrogen Concentration in Liquid Fertilizer Contaminated Areas using Hyperspectral Images (초분광 영상을 이용한 액비 오염지역의 질산성질소 농도 추정)

  • Lim, Eun Sung;Kim, I Seul;Han, Soo Jeong;Lim, Tai Yang;Song, Wonkyong
    • Journal of the Society of Disaster Information
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    • v.16 no.3
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    • pp.542-549
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    • 2020
  • Purpose: As nitrate nitrogen produced during fermentation of liquid fertilizer is a pollution indicator of water, in this study, four research areas where liquid fertilizer was sprayed were selected, and a model was designed to estimate the concentration of nitrate nitrogen pollution. Method: Prior to shooting on site, a spectrum library was constructed by dividing the ratio of liquid fertilizer into 5 groups: 0%, 25%, 50%, 75%, and 100%. PLSR (Partial least squares regression) method was applied to hyperspectral images acquired in the study area based on the aspect of spectrum. Result: The behavior of nitrate nitrogen was confirmed by 1st and 2nd differentiation of the spectrum of the constructed liquid fertilizer. PLSR concentration estimation modeling was implemented using images from field experiments and compared with actual concentration of nitrate nitrogen. Conclusion: When comparing the PLSR concentration estimation model with the actual concentration of nitrate nitrogen, it was measured that the detection is possible in high concentration areas where the concentration of nitrate nitrogen is 70mg/kg or more.

Regional Background Levels of Carbon Monoxide Observed in East Asia during 1991~2004 (1991~2004년 동아시아에서 관측한 일산화탄소의 지역적 배경 농도)

  • Kim, Hak-Sung;Chung, Yong-Seung
    • Journal of the Korean earth science society
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    • v.27 no.6
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    • pp.643-652
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    • 2006
  • Data of the carbon monoxide concentration observed in Mt. Waliguan in China (WLG), Ulaan Uul in Mongolia (UUM), Tae-ahn Peninsula in Korea (TAP), and Ryori in Japan (RYO) were analyzed for a long period between 1991 and 2004. The annual average concentration of carbon monoxide was the highest at TAP $(233{\pm}41ppb)$ followed by $RYO(171{\pm}36ppb),\;UUM(155{\pm}26ppb),\;and\;WLG(135{\pm}22ppb)$. The seasonal variations being high in spring and low in summer were observed in other areas of Eastern Asia except WLG. TAP was high in carbon monoxide concentration in all seasons compared to WLG, UUM and RYO and shows wide distribution of concentration in the histogram, which is caused by the influence of large-scale air pollution due to its downwind location close to the East Asian continent, China in particular. Also, our data was compared with data measured at Mauna Loa (MLO) in Hawaii. According to the origin of the isentropic backward trajectory and its transport passage, carbon monoxide concentration observed in TAP was analyzed as follows: continental background airflows (CBG) were $216{\pm}47ppb$; regionally polluted continental airflows (RPC) were $316{\pm}56ppb$; Oceanic background airflows (OBG) were $108{\pm}41ppb$; and Partly perturbed oceanic airflows (PPO) were $161{\pm}6ppb$. The high concentration of carbon monoxide in TAP is due to the airflow from East Asian continent origin rather than that from the North Pacific origin. Especially, RPC which passes through the eastern China appeared to be the highest in concentration in spring, fall, and winter. However, OBG was affected by the North Pacific air mass with a low carbon monoxide concentration in summer. The NOAA satellite images and GEOS-CHEM model simulation confirmed a large-scale air pollution event that was in the course of expansion from southeastern China bound to the Korean Peninsula and the Korea East Sea by way of the Yellow Sea.