• Title/Summary/Keyword: concentrated cells

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Exploitation of the biologically active components in Youngia sonchifolia Max (고들빼기 생리활성물질의 검색)

  • Shin, Soo-Cheol
    • Applied Biological Chemistry
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    • v.36 no.2
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    • pp.134-137
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    • 1993
  • Yongia sonchifolia Max. has been used as raw materials of traditional Kimchi and medicinal herb in Korea. This study was performed to investigate biologically active components in the plant. First, the writer carried out the experiment of antitumor screening test against Sarcoma-180A and the cytotoxic activity against Chinese hamster V-79 cells with methanol extract of the plant. And the aqueous solution of the extract from roots of Youngia sonchifolia Max. was partitioned into n-hexane. The concentrated extract of n-hexane layer was chromatographed on silica gel column and developed with n-hexane and ethylacetate. Two yellow elutes, on concentration, were recrystallized from ethylacetate, and the $R_f$ value of TLC of the crystal was 0.43. After analysis by $^{1}H-NMR$, $^{13}C-NMR$ and MS to confirm the structure, the author could identify the compound as bauerenyl acetate, a naturally occurring pentacyclic triterpene. The crystal was colorless plate and m.p. was $280{\sim}282^{\circ}C$.

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Isoprenylated flavonoids from the root bark of Morus alba L. and their inhibition effect on NO production in LPS-induced RAW 264.7 cells

  • Jung, Jae-Woo;Ko, Jung-Hwan;Ko, Won-Min;Park, Ji-Hae;Baek, Yun-Su;Kim, Youn-Chul;Baek, Nam-In
    • Journal of Applied Biological Chemistry
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    • v.60 no.2
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    • pp.109-111
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    • 2017
  • The root bark of Morus alba L. were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH, and $H_2O$ fractions. The repeated silica gel ($SiO_2$), octadecyl $SiO_2$ (ODS), and Sephadex LH-20 column chromatographies of the EtOAc fraction led to isolation of 12 phenolic compounds. The chemical structures of the compounds were determined as sanggenol Q (1), sanggenol A (2), sanggenol L (3), kuwanon T (4), cyclomorusin (5), sanggenon F (6), sanggenol O (7), sanggenon N (8), sanggenon G (9), mulberrofuran G (10), mulberrofuran C (11), and moracin E (12). All isolated compounds were evaluated for inhibit lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophages.

Experimental and mathematical evaluation of solar powered still equipped by nano plate as the principle stage of zero discharge desalination process

  • Jadidoleslami, Milad;Farahbod, Farshad
    • Advances in Energy Research
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    • v.4 no.2
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    • pp.147-161
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    • 2016
  • To start with, finding a sustainable method to produce sweet water and electricity by using renewable energies is one of the most important issues at this time. So, experimental and theoretical analysis of the performance of a closed solar powered still, which is jointed to photovoltaic cells and vacuum pump and equipped by nano plate, as the principle stage of zero discharge desalination process is investigated in this project. Major goal of this work is to reuse the concentrated brine of the Mobin petrochemical complex in order to produce potable, sweet water from effluent saline wastewater and generating electricity in the same time by using solar energy instead of discharging them to the environment. It is observed the increase in brackish water temperature increases the average daily production of solar desalination still considerably. Therefore, the nano plate and vacuum pump are added to augment the evaporation rate. The insolation rate, evaporation rate, the average brackish temperature, ambient temperature, density are investigated during a year 2013. In addition to obtain the capacity of solar powered still, the highest and lowest amount of water and electricity generation are reported during a twelvemonth (2013). Results indicate the average daily production is increased 16%, which represents 7.78 kW.h energy saving comparing with traditional solar still.

p53 Protein Expression Area as a Molecular Penumbra of Focal Cerebral Infarction in Rats

  • Hong, Hyun-Jong;Park, Seung-Won;Kim, Young-Baeg;Min, Byung-Kook;Hwang, Sung-Nam;Suk, Jong-Sik
    • Journal of Korean Neurosurgical Society
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    • v.38 no.4
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    • pp.293-298
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    • 2005
  • Objective : The authors investigate the spatial characteristics of apoptotic genes expressed around the focal cerebral infarction, and attempted to explain the penumbra with them. Methods : A delayed focal cerebral infarction was created in twelve adult Sprague-Dawley rats. We performed the immunohistochemical staining for the apoptosis, bcl-2 and p53 proteins and measured the local cerebral blood flow [CBF] at the infarction core area and peri-infarct area pre- and intra-operatively. The peri-infarct area was divided into six sectors by distance from the infarction border. Results : The size [$mm^2$] of apoptosis, bcl-2, and p53 areas were $3.1{\pm}1.2$, $4.7{\pm}2.1$, and $6.8{\pm}2.4$, respectively. Apoptosis, bcl-2 or p53 positive cells were concentrated at the peri-infarct area adjacent to the infarction core. Their numbers reduced peripherally, which was inversely proportional to the local CBF. The p53 area seems to overlap with and larger than the ischemic penumbra. Conclusion : The p53 positive area provides a substitutive method defining the penumbra under the molecular base of knowledge.

A Study on the Fixed-Concentrating Hybrid Panel using Reflector (반사판을 이용한 고정식 집속형 복합 Panel에 대한 연구)

  • Kim, Kiu-Jo;Kim, Wan-Tae;Lee, Tae-Ho;Yoo, Hung-Chul;Huh, Chang-Su
    • Journal of the Korean Solar Energy Society
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    • v.21 no.2
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    • pp.1-8
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    • 2001
  • The most effective methods for utilizing solar energy are to use the sunlight and solar thermal energy such as hybrid panel simultaneously and to use concentrator. From such a view point systems using various kinds of photovoltaic panels are constructed in the world. However, there has not been a hybrid panel with a concentrator. If the sunlight is concentrated on solar cell, cell conversion efficiency increases and the temperature of the solar cell s increases. As the temperature of the solar cells increases, the cell conversion efficiency gradually decreases. For maintaining the cell conversion efficiency constant, it is necessary to keep solar cell at low temperature. In this paper, after designing a concentration rate for concentrating, we propose a model for cooling the cell and for using wasted heat. And, we compare it with conventional panels after calculating the electrical and thermal efficiency, using the energy balance equation.

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A Study of Zero Energy Building Verification with Measuring and Model-based Simulation in Exhibition Building

  • Ha, Ju-wan;Park, Kyung-soon;Kim, Hwan-yong;Song, Young-hak
    • Architectural research
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    • v.20 no.3
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    • pp.93-102
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    • 2018
  • With the change in Earth's ecosystems due to climate change, a number of studies on zero energy buildings have been conducted globally, due to the depletion of energy and resources. However, most studies have concentrated on residential and office buildings and the performance predictions were made only in the design phase. This study verifies the zero-energy performance in the operational phase by acquiring and analyzing data after the completion of an exhibition building. This building was a retention building, in which a renewable energy system using a passive house building envelope, solar photovoltaic power generation panels, as well as fuel cells were adopted to minimize the maintenance cost for future energy-zero operations. In addition, the energy performance of the building was predicted through prior simulations, and this was compared with actual measured values to evaluate the energy performance of the actual operational records quantitatively. The energy independence rate during the measurement period of the target building was 123% and the carbon reduction due to the energy production on the site was 408.07 tons. The carbon reduction exceeded the carbon emission (331.5 tons), which verified the carbon zero and zero-energy performances.

The study about the medical treatment and efficacy of Colostral milk (문헌고찰을 통한 Colostral milk의 의학적기전(醫學的機轉)과 효능(效能)에 관한 연구(硏究))

  • Han, Sung
    • Journal of the Korean Institute of Oriental Medical Informatics
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    • v.14 no.2
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    • pp.59-72
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    • 2008
  • It makes study on the medical treatment and efficacy of Colostral milk. It based on the established treatises and books, in order to studying about the literature of colostral milk. It makes a through study on the medical treatment mechanism and efficacy of colostral milk, the results as fllows. 1. Colostral milk is some special things for baby newborn, and the other men and women of course. It is low in fat, and high in carbohydrates, protein, and antibodies to help keep your body healthy. Colostral milk is easy to digest, and it is low in volume, but high in concentrated nutrition for the newborn and men and women. 2. Colostral milk provides some of living cells which will defend babies against many harmful agents. The immune factors is much higher in colostral milk than in nature milk. Colostral milk works as a vaccine. It contains some quantities of an antibody called secretory immunoglobulin A Which is a important substance to the baby and men, 3. Colostral milk has a role to play in the baby's gastrointestinal tract. A newborn's intestines are permeable. The colostral milk makes the gastrointestinal tract in general. In addition, colostral milk contains a lot of leukocytes.

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A Study of Laser Patterning for $SiO_2$ Thin Film of Crystalline Solar Cells (결정질 태양전지 $SiO_2$ 박막의 Laser Patterning에 관한 연구)

  • Lee, C.S.;Lee, J.C.;Kim, K.S.;Kang, H.S.
    • Laser Solutions
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    • v.14 no.3
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    • pp.1-6
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    • 2011
  • Globally, the interest of renewable energy has become an upsurge. Especially, the solar industry is the one which is getting rapid growth rate. Many of researchers have been undertaking to improve the efficiency of solar cell to accomplish grid parity. The most of research has been concentrated on two methods, one on the selective emitter and the other is on LBSF (Local Back Surface Field) formation. Laser patterning will be needed to eliminate the thin film to form selective emitter and LBSF of solar cell. This paper reports some experimental results in laser patterning process for high-efficiency crystalline solar cell manufacturing. The experimental results indicate that the patterning quality depends on the average power and repetition rate of laser. The experimental results prove that the laser patterning process is an advantageous method to improve the efficiency of solar cell.

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An Acute Outbreak of Calf Salmonellosis Caused by Salmonella dublin (Salmonella dublin에 의한 소의 살모넬라증의 발생)

  • Bak, Ung-bok;Han, Hong-ryul;Han, Jeong-hee
    • Korean Journal of Veterinary Research
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    • v.27 no.1
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    • pp.69-76
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    • 1987
  • An epizootic of calf diarrhea occurred in an extensive dairy farm located in Gangwondo province in December of 1985. The patients showing fever, chills, complete anorexia and watery diarrhea sometimes dysentery were concentrated among the calves ranged from 8 days to 3 months old and many of them died in acute course. The five carcases were examined by pathological and bacteriological means. The predominant gross lesions were edematous swelling of the mesenteric lymph nodes, profuse catarrhal or diphtheritic enteritis and extensive purulent bronchopnenomonia or lobar fibrinous pneumonia. Microscopically the lesions of the liver and spleen were characterized by coagulative necrotic foci and granulomatous nodules. The spleen and lymph node showed also proliferative changes of reticular cells and involution of intestinal lymphatic nodules were noticed. The strains of Salmonella(S) species isolated from the liver, spleen, bile juice, peritoneal fluid and thoracic fluid were identified serologically as S. dublin. These clinical and pathological findings of the disease were those of acute enteritis form of salmonellosis and characterized by high morbidity and mortality among the calf herd. The report also signifies the first description of an epizootic of bovine salmonellosis caused by S. dublin in Korea.

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Pathological characteristics on porcine enteritis associated with porcine circovirus type 2 in Jeju (제주지역의 돼지 써코바이러스 2형 관련 장염에 대한 병리학적 특징)

  • Mun, Seong-Hwan;Yang, Hyoung-Seok;Kim, Jae-Hoon
    • Korean Journal of Veterinary Research
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    • v.60 no.3
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    • pp.155-161
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    • 2020
  • From 2006 to 2009, 50 pigs suspected of enteritis associated with porcine circovirus type 2 (PCV-2) (EAPC) were collected. Gross and histopathologic examinations and immunohistochemistry (IHC) were performed on the small intestine of 50 pigs. The pigs with EAPC were concentrated in grower pigs (68%), and diarrhea (84%) was the most common clinical sign. Grossly, the walls of the small intestine were thickened, and mesenteric lymph nodes were enlarged. The histopathologic features in the small intestine with EAPC were characterized by lymphoid depletion, histiocytic infiltration, and formation of basophilic grape-like inclusion bodies and multinucleated giant cells in gut-associated lymphatic tissue (GALT) and lamina propria. According to IHC, PCV-2 antigens were more intense and distributed widely in GALT (98%) than lamina propria (82%) of the small intestine. Co-infection with other enteric pathogens was detected in 26 pigs (52%). Twenty-four pigs (48%) were confirmed to be a PCV-2 single infection. Collectively, infected PCV-2 in the small intestine may induce an immunosuppressive status of individuals and then allow secondary co-infections in the digestive system. This study shows that PCV-2 can induce diarrhea and enteric lesions in pigs without a co-infection of other enteric pathogens.