Journal of Korean Neurosurgical Society

The Korean Neurosurgical Society (대한신경외과학회)
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p53 Protein Expression Area as a Molecular Penumbra of Focal Cerebral Infarction in Rats

  • Hong, Hyun-Jong (Department of Neurosurgery, College of Medicine, Chung-Ang University) ;
  • Park, Seung-Won (Department of Neurosurgery, College of Medicine, Chung-Ang University) ;
  • Kim, Young-Baeg (Department of Neurosurgery, College of Medicine, Chung-Ang University) ;
  • Min, Byung-Kook (Department of Neurosurgery, College of Medicine, Chung-Ang University) ;
  • Hwang, Sung-Nam (Department of Neurosurgery, College of Medicine, Chung-Ang University) ;
  • Suk, Jong-Sik (Department of Neurosurgery, College of Medicine, Chung-Ang University)
  • Published : 2005.10.28
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Abstract

Objective : The authors investigate the spatial characteristics of apoptotic genes expressed around the focal cerebral infarction, and attempted to explain the penumbra with them. Methods : A delayed focal cerebral infarction was created in twelve adult Sprague-Dawley rats. We performed the immunohistochemical staining for the apoptosis, bcl-2 and p53 proteins and measured the local cerebral blood flow [CBF] at the infarction core area and peri-infarct area pre- and intra-operatively. The peri-infarct area was divided into six sectors by distance from the infarction border. Results : The size [$mm^2$] of apoptosis, bcl-2, and p53 areas were $3.1{\pm}1.2$, $4.7{\pm}2.1$, and $6.8{\pm}2.4$, respectively. Apoptosis, bcl-2 or p53 positive cells were concentrated at the peri-infarct area adjacent to the infarction core. Their numbers reduced peripherally, which was inversely proportional to the local CBF. The p53 area seems to overlap with and larger than the ischemic penumbra. Conclusion : The p53 positive area provides a substitutive method defining the penumbra under the molecular base of knowledge.

Keywords

References

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