• 제목/요약/키워드: concentrated cell

검색결과 323건 처리시간 0.025초

수종의 한약재가 Streptozotocin으로 유발된 고혈당 생쥐에 미치는 영향 (The Effects of Several Herbal Medicine Concentrated Solution on the Hyperglycemic Mice Induced with Streptozotocin)

  • 채중원;주숙현
    • 대한한방소아과학회지
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    • 제20권1호
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    • pp.165-180
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    • 2006
  • Objective : This study has been carried out to understand the effect of several herbal medicine concentrated solution on the hyperglycemic mice Induced with streptozotocin(STZ). Methods : The 60 mg/kg of STZ injection into mice twice by 24 h interval and then 120 mg/kg of STZ injection again 3 days after the earlier injection. Control group was subjected to natural recovery, however, treated groups were fed 0.2 ml of several herbal medicine-concentrated solution (PA (x2, several herbal medicine-concentrated solution 1 group); PB (x4 several herbal medicine-concentrated solution 2 group) daily for 6 weeks. Result : The weight of PA was higher than that of control, but weight of PB was lower than control. The blood level of control increased continuously, reaching to 350mg/dL after 6 weeks, however, PA and PB showed a fast reduction of blood glucose. In blood glucose tolerance test, PA and PB showed better resistance than control. The GOT level in significantly(p<0.05) decreased in PA and PB compared with control group. The BUN and creatinine levels are significantly(p<0.01) decreased in PA compared with control group. Feeding of several herbal medicine-concentrated solution in a concentration of PA had an efficient effect on regeneration or recovery of Langerhans islet and ${\beta}-cell$ damaged by STZ. More Langerhans islet and high insulin-immunohistochemical resistance were observed in PA compared with control, but they were higher in PB than in PA. The number of Langerhans islet ${\beta}-cell$ and Langerhans islet. Conclusions : The result from the six weeks of observation demonstrates that the several herbal medicine concentrated solution have a positive effect of lowering the level of blood sugar and they increased insulin concentration. They have an effect for recovery of pancreas tissue and recovery of kidney, liver function from a diabetes mellitus.

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Encapsulation of Whole Cell CGTase from Concentrated Broth Solution

  • Park, Joong-Kon;Sohn, Joon-Hyuk;Park, Hyung-Woo;Lee, Yong-Hyun
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제6권1호
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    • pp.67-71
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    • 2001
  • Most of the Cyclodextrin glucanotransferase (Gtases) which have been produced from B. subtilis were found to be excreted from the cells during cultivation. Immobilized whole cell CGTase from B. subtilis was prepared by encapsulating the broth solution which had been concentrated ten times with a rotary vacuum evaporator. Cyclization activity of CGTase was reduced by about 10% during the concentrating process, however, its transglycosylation activity, to convert xylitol to glucosyl-xylitol, using dextrin as glucosyl donor, increased by a factor of 3 or 5.

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2차 광학계가 필요없는 프레넬 렌즈를 이용한 중집광 광학계 시뮬레이션 (Fresnel lens optics simulation with middle sized linear concentration without secondary optics)

  • 강성원;김용식;심창호
    • 한국태양에너지학회:학술대회논문집
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    • 한국태양에너지학회 2011년도 추계학술발표대회 논문집
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    • pp.27-33
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    • 2011
  • HCPV(High Concentrated PV) systems have well known for CPV market all over the world. Low concentration type silicon based modules have been introduced in the market. But low cost of standard flat silicon modules made them useless nowadays. High cost of compound semiconductor solar cell reduced cost effective cpv module production than that of recently silicon solar cell. In order to overcome increasing cost of CPV module, we study middle concentration type fresnel lens simulation using concentrated type silicon based solar cell. Linear type fresnel lens made production of CPV module without secondary optics such as light pipe or light tunnel. This type of fresnel lens design makes more cost effective solution for cpv niche market.

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미세조류 Dunaliella bardawil의 고농도 세포배양 (High Cell Density Culture of Micro-algal Dunaliella bardawil)

  • 정욱진;왕만식;최승인;정병철;김주곤
    • KSBB Journal
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    • 제14권2호
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    • pp.160-166
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    • 1999
  • 본 연구에서는 ($\beta$-carotene 생산균주인 미세조류 Dunaliella bardawil을 사용하여 batch flask에서 미세조류의 고농도세포에 관한 최적배양조건(미량원소, pH, agitation speed, nitrate, phosphate, carbon source)을 확립하고자 하였다. 미량원소는 5X 배지에서 교반하였을 때 비생장속도는 $0.0l3hr^{-l}$와 세포농도는 $4.9{\times}10^6$ cells/mL로서 IX. 3X, lOX 배지에서 배양한 것보다. 약 46%, 18%, 69% 높은 세포수율을 얻었으며 세포배양시 교반한 경우, pH는 80에서 최대 세포농도를 얻었다. 초기 nitrate ($KNO_3$)와 phosphate($KH_2PO_4$)의 영향을 조사한 결과 미세조류 생장에 중요한 영양분으로서 질소원의 주입은 매우 효과적임을 확인하였다. 또힌 탄소원으로서 250mM의 $NaHCO_3$$CO_2$ 가스를 동시에 사용한 배양조건이 500mM $NaHCO_3$만을 탄소원으로 사용한 실험에 비하여 32% 증가된 세포농도를 나타내었다. light는 white light의 경우 blue light보다 세포생장에 적합하였다. 질소원을 이용한 유기배양시 2회의 nitrate주입만으로써 배양 198hr에 $8.955{\times}10^6$cell/mL의 고농도의 세포를 얻었다.

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장기 Outdoor Test를 통한 CPV와 PV 모듈의 발전량 비교분석 (A study of Comparative Analysis of CPV and PV Module through Long-term Outdoor Testing)

  • 김민수;이유리;조민제;오수영;정재학
    • Current Photovoltaic Research
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    • 제5권1호
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    • pp.33-37
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    • 2017
  • Today, photovoltaic power generation mostly uses Si crystalline solar cell modules. The most vulnerable part of the Si solar cell module is that the power generation decreases due to the temperature rise. But, it is widely used because of low installation cost. In the solar market, where Si crystalline solar cell modules are widely used. The CPV (Concentrated Photovoltaic) module appeared in the solar market. The CPV module reduces the manufacturing cost of the solar cell by using non-Si in the solar cell. Also, there is an advantage that a rise in temperature does not cause a drop in power generation. But this requires high technology to install and has a disadvantage that the initial installation cost is expensive compared to normal Si solar cell module. So that we built a testbed to see these characteristics. The testbed was used to measure the amount of power generation in a long-term outdoor environment and compared with the general Si solar cell module.

Studies on standardization and characterization of recombinant interferon alia

  • Kim, Gi-Hyun;Shin , Won;Jung , Ja-Young;Park, Young-Ju;Joung , Jee-Won;Oh, Il-Ung;Jin, Jae-Ho;Kim, Seo-Mi;Jung , Sang-Mi
    • 대한약학회:학술대회논문집
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    • 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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    • pp.329.2-330
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    • 2002
  • This study was intended to establish test methods equivalent to those of "Interferon alfa-2 concentrated solution" monograph in European Pharmacophoeia(EP). Two recombinant interferon alfa concentrated solutions manufactured in Korea were tested according to the monograph of EP. Tests of identification(biological activity. isoelectric focusing. SDS-PAGE under reducing condition, peptide mapping). related proteins. impurities of moducular masses differing from that of interferon alfa-2(SDS-PAGE under reducing and non-reducing condition). bacterial endotoxin, protein, potency, host-cell-derrved proteins. and host-cell-derived DNA were performed in the laboratories of manufacrues and division of biotechnology. KFDA. The results of this study showed that specitications of interfenon alfa concentrated solutions manufactured in Korea were within the aceptance criteria of EP. Based on the study. specitications and test methods for interferon alfa concentrated solution can be established according to the monograph of EP suggesting the revision of Minimum requirements for biological products

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Improved Production of Curdlan with Concentrated Cells of Agrobacterium sp.

  • Jung, Dae-Young;Cho, Young-Su;Chung, Chung-Han;Jung, Dai-Il;Kim, Kwang;Lee, Jin-Woo
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제6권2호
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    • pp.107-111
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    • 2001
  • The addition of a limited concentration of yeast extract to a minimal salt medium (MSM) enhanced cell growth and increased the production of curdlan whereas nitrogen-limitation was found to be essential for the higher production of curdlan by Agrobacterium sp. ATCC 31749. As the amount of the inoculum increased, the cell growth as well as the production of curdlan also increased in the MSM without a nitrogen source. The cell growth and production of curdlan increased as the initial pH of the medium decreased as low as 5.0. The conversion rate and concentration of curdlan from 2% (w/v) glucose in the MSM with concentrated cells under nitrogen deletion was 67% and 13.4 g/L, respectively. The highest conversion rate of curdlan under the conditions optimized in this study was 71% when the glucose concentrations was 1% (w/v).

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JTAG을 이용한 휴대폰 포렌식 데이터 수집 (Forensic Data Acquisition on Cell Phone using JTAG Interface)

  • 김건우;류재철
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2008년도 하계종합학술대회
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    • pp.333-334
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    • 2008
  • With the role of cell phones in today's society as a digital personal assistant as well as the primary tool for personal communication, it is possible to imagine the involvement of cell phones in almost any type of crime. The progression of a criminal investigation can hinge on vital clues obtained from a cell phone. This paper will be concentrated on CDMA system phones and focus on the data extraction for cell phone forensics. Especially, the data acquisition method of JTAG interface access to memory chip will be covered.

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고집광 태양광 발전을 위한 렌즈 및 광 파이프 특성 시뮬레이션 (Simulation of Characteristics of Lens and Light Pipe for High Concentration Solar PV System)

  • 유광선;신구환;차원호;명로훈;김용식;정호윤;김동균;강기환
    • 한국태양에너지학회:학술대회논문집
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    • 한국태양에너지학회 2011년도 춘계학술발표대회 논문집
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    • pp.282-286
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    • 2011
  • The artificial increase in the solar intensity incident on solar cells using lenses or mirrors can allow solar cells to generate equivalent power with a lower cost. In application areas of Fresnel lenses as solar concentrators, several variations of design were devised and tested. Some PV systems still use commercially available flat Fresnel lenses as concentrators. In this study, we designed and optimized flat Fresnel lens and the 'light pipe' to develop 500X concentrated solar PV system. We performed rigorous ray tracing simulation of the flat Fresnel lens and light-pipe. The light-pipe can play imporatant roles of redistributing solar energy at the solar cell and increase the mechanical tolerance so that it can increase the lifetime of the high-concentration solar PV system and decrease the cost of manufacturing. To investigate the sensitivity of the solar power generated by the concentrated solar PV according to the performance of lens and light pipe, we performed raytracing and executed a simulation of electrical performance of the solar cell when it is exposed to the non-uniform illumination. We could conclude that we can generate 95 % or more energy compared with the energy that can be generated by perfectly uniform illumination once the total energy is given the same.

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Development of concentrated lactic starter cultue II. Effect of Agitation & Aeration on Cell Growth and Lactic Acid Formation of L. bulgaricus

  • 이상기;박무영
    • 한국미생물생명공학회:학술대회논문집
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    • 한국미생물생명공학회 1976년도 제8회 학술발표회
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    • pp.187.3-187
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    • 1976
  • 건조 유산균 starter (Lyophillized lactic sarter) 제조 과정 중 동결건조에 의한 viability의 저하를 방지하는 방법의 하나로서 유산균을 농축 (concentrate) 시켰다. 유산균을 농축시키기에 앞서 cell의 농도를 높여줄 필요가 있었으므로 Cell의 증식에 영향을 미치는 요인으로 agitation 및 aeration을 채택하여 그것이 cell의 증식 및 lactic acid producing ability에 미치는 영향을 조사한 결과 다음과 같은 사실을 알 수 있었다.(중략)

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