Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Encapsulation of Whole Cell CGTase from Concentrated Broth Solution

  • Park, Joong-Kon (Department of Chemical Engineering, Kyungpook National University) ;
  • Sohn, Joon-Hyuk (Department of Chemical Engineering, Kyungpook National University) ;
  • Park, Hyung-Woo (Department of Chemical Engineering, Kyungpook National University) ;
  • Lee, Yong-Hyun (Department of Genetic Engineering, Kyungpook National University)
  • Published : 2001.01.01
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Abstract

Most of the Cyclodextrin glucanotransferase (Gtases) which have been produced from B. subtilis were found to be excreted from the cells during cultivation. Immobilized whole cell CGTase from B. subtilis was prepared by encapsulating the broth solution which had been concentrated ten times with a rotary vacuum evaporator. Cyclization activity of CGTase was reduced by about 10% during the concentrating process, however, its transglycosylation activity, to convert xylitol to glucosyl-xylitol, using dextrin as glucosyl donor, increased by a factor of 3 or 5.

Keywords

References

  1. Biotechnol. Lett. v.14 Synthesis of glucosyl-inositol using a CGTase, isolation and characterization of the positional isomers, and assimilation properties for instestinal bacteria Sato, M.;K. Nakamura;H. Nagano;Y. Yagi;K. Koizumi
  2. Biotechnol. Lett. v.13 Synthesis of novel sugars, oligoglucosyl-inositols, and their growth stimulating effect for Bifidobacterium Sato M.;T. Matsuo;N. Orita;Y. Yagi
  3. Biosci. Biotech. Biochem. v.56 Transglucosylation catalyzed by sucrose phosphorylase from Leuconostoc mesenteroides and production of glucosyl-xylitol Kitao, S.;H. Sekine
  4. J. Microbiol. Biotechnol. v.7 Synthesis of glucosyl-sugar alcohols using glycosyltransferases and structural identification of glucosyl-maltitol Kim T.W.;D.C. Park;Y.H. Lee
  5. Kor. J. Appl. Microbiol. Biotechnol. v.26 Synthesis of transglucosylated xylitol using cyclodextrin glucanotransferase and its stimulating effect on the growth of Bifidobacterium Kim T. W.;D. C. Park;Y. H. Lee
  6. Biotechnology v.7a Immobilized cell systems Brodelius, P.;E.J. Vandamme;J.F. Kennedy(ed.)
  7. Chem. Eng. J. v.61 Biosysthesis of cyclodextrin glucoxyltransferase and β-cyclodextrin by Bacillus macerans 314 and properties of the crude enzyme Ismail A.S.;U.I. Sobieh;A.F. Abdel-Fattah
  8. Kor. J. Biotechnol. Bioeng. v.15 Production of glucosyl-xylitol using encapsulated whole cell CGTase Park, J.K.;H.W. Park;Y.H. Lee
  9. Biotechnol. Tech. v.7 Microencapsulation of yeast cells in the calcium alginate membrane Cheong S.H.;J.K. Park;B.S. Kim;H.N. Chang
  10. J. KIChE v.33 L-lysine production using encapsulated Corynebacterium glutamicum Cheong S.H.;T.J. Lee;J.K. Park;H.N. Chang
  11. Biotechnol. Bioeng. v.51 Microencapsulation of recombinant Saccharomyces cerevisiae cells with invertase activity in the liquid core alginate capsules Chang H.N.;G.H. Seong;I.K. Yoo;J.K. Park;J.H. Seo
  12. Kor. J. Biotechnol. Bioeng. v.11 Encapsulation of whole cell β-galactosidase of Escherichia coli Lee B.H.;J.K. Park
  13. Bioprocess Eng. v.17 The effect of oxygen transfer on the activity of encapsulated whole cell β-galactosidase park J.K.;G.S. Jeong;H.N. Chang
  14. Bioprocess Eng. v.19 The characteristics of encapsulated whole cell β-galactosidase Oh, C.Y.;J.K. Park
  15. US Patent 4,309,505 Smith, J.A.;D. Grove;S.J. Luenser;L.G. Park
  16. Enzyme production of oligosaccharides from Aureobasidium pullulans Yun, J.W.
  17. Enzyme Microb. Technol. v.22 Bacterial cyclodextrin glucanotransferase Tokova A.
  18. Biotechnol. Adv. v.18 Microencapsulation of microbial cells Park, J.K.;H.N. Chang
  19. Proceedings of APBioChEC'99 Kinetic mechanism of cyclodextrin glucanotransferase reaction in supercritical carbon dioxide Ko, E.Y.;C.Y. Park;Y.W. Ryu;C. Kim
  20. Biotechnol. Lett. v.7 Enzymatic catalysis in a supercritical fluid Randolph, T.W.;H.W. Blanch;J.M. Prausnitz;C.R. Wielke
  21. Appl. Biochem. Biotechnol. v.11 Enzymatic reactions in supercritical gases Hammond, D.A.;M. Karel;A.M. Klibanov
  22. Trends Biotechnol. v.8 Biochemical reactions in supercritical fluids Nakamura, K.
  23. Agric. Biol. Chem. v.51 Effect of treatment with supercritical carbon dioxide on enzymatic activity Taniguchi, K.;M. Kamihira;T. Kobayashi