• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.62-65
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• 2002

We have previously shown that the addition of silkworm hemolymph to a culture medium increases the longevity of insect and mammalian cells by inhibiting apoptosis. This indicates that the component which inhibits apoptosis is contained in the silkworm hemolymph, The apoptosis-inhibiting component was isolated from silkwonn hemolymph and characterized in our previous study. A database search using the N-terminal amino acid sequence of this component as a template resulted in a 95% homology with a low molecular weight lipoprotein, the so called ’30K protein' of unknown function. In this study, the 30K protein gene was expressed in mammalian and insect cells to confirm the apoptosis-inhibiting effect. The overexpression of 30K protein in mammalian cell inhibited the staurosporin-induced apoptosis by the prevention of the activation of caspase 3. Using an Autographa californicanuclear polyhedrosis virus (AcNPV) system, the 30K protein was overexpressed also in insect cells. The expression of the 30K protein increased the longevity of baculovirus-infected insect cells by inhibiting apoptosis. These results suggest that the 30K protein is a novel anti-apoptotic protein.

• Journal of Ginseng Research
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• 제15권3호
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• pp.167-170
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• 1991

Radiation protective fraction was Isolated and partially purified from Korean white ginseng. The effect of the fraction was studied on the cell survival of W-damaged CHO-Kl cells. As a result, it was found that the fraction increased the survival rate of damaged cells significantly within the dose range of which cytotoxicity did not appear This fraction was separated into two parts by adding butanol, namely the precipitated protein component and the butanol extract. Damaged cells were treated with each of these components and their survival rates were measured. The protein component demonstrated significant increase in the survival rates, while the butanol extract showed no such increment. These results suggest that the radiation protective effect of the ginseng fraction is originated from the butanol-precipitated protein component, not from the butanol-soluble compounds.

• 한국식품영양학회지
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• 제11권5호
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• pp.536-541
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• 1998

This study was carried out to investigate the changes of characteristics of myofibrillar protein for the vacuum chilled, the air frozen or the rechilled Holstein beef loin. The vacuum chilled beef was stored at 1$^{\circ}C$ and the air frozen beef was stored at -20$^{\circ}C$ for 60 days. The rechilled beef was restored for 3 days at 1$^{\circ}C$ by using the vacuum chilled or the air frozen beef. Myofibrillar protein extractability, 30,000 dalton component content and Mg-ATP ase activity for the vacuum chilled beef were higher than those of the air frozen beef. Each parameters increased significantly for the vacuum chilled beef after the 20 days storage, but there was no significant difference for the vacuum chilled beef after the 20 days storage, but there was no significant difference for the air frozen beef during the 60 days storage. By the rechilling process, myofibrillar protein extractability of the vacuum chilled and the frozen beef were not significant difference. The 30,000 dalton component of the vacuum chilled beef was showed not significant increment by rechilling, but the frozen beef was showed significant increment by rechilling. The Mg-ATPase activity of myofibrillar protein of the vacuum chilled beef was not changes by rechilling, but the frozen beef after the 20 days storage was significant increment by rechilling.

• 한국응용과학기술학회지
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• 제3권1호
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• pp.23-31
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• 1986

Several authors reported that vegetable proteins cause to decrease serum cholesterol and reported the effect of limiting amino acid balance in dietary protein on the blood component. This study was designed to observe growth rate and blood component in 1% cholesterol-fed male rats intake pure isolated soy proteins and methionine for 4 weeks. The diets were supplemented with either 20% casein or isolated soy protein as dietary protein sources and methionine as limiting amino acid of isolated soy protein and casein protein. The results were as follows; 1) The body weight gain was the greatest in casein groups. 2) Serum total cholesterol concentration was significantly increased by casein groups and slightly decreased by isolated soy protein groups. 3) Serum HDL-cholesterol concentration was significantly increased by isolated soy protein groups than control group. 4) Serum total lipid and triglyceride concentrations were significantly decreased by isolated soy protein groups than control group. 5) Serum glucose concentration was significantly increased by isolated soy protein plus 0.8% methionine than control group. 6) Serum total protein and albumin concentrations were significantly increased by all experimental diets groups than control group. Especially, casein groups were the most increased in all experimental diets groups.

• Journal of Community Nutrition
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• 제8권1호
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• pp.3-8
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• 2006

This retrospective study was designed to evaluate the nutrient contributions of the five meal components of school lunch menus planned for elementary students in two school districts (District A and B) in the Midwestern state of the United States. The 4-week cycle menu was planned for two time periods (Period 1 and Period 2) following guidelines for NuMenus and general menu planning principles. Menu components of planned and served menus for two time periods were analyzed using $Nutri-Kids^{TM}$. No significant differences in the nutrient content of between Periods 1 and 2 were found for District A. District B served significantly more vitamin A and total fat in Period 1 and significantly more calories, iron, vitamin A, protein, and total fat in Period 2 than was planned. The major nutrients provided by the entree component included protein, calories, cholesterol, total fat, saturated fat, and sodium. Milk was an important source of calcium and provided approximately one-third of the total protein and vitamin A in the meal. The vegetable/fruit component was the major source of vitamins A and C. The grain/bread component provided approximately 20% of the carbohydrates among five meal components. The miscellaneous component affected the sodium and fat content of the menus. Menu planners can use the results of this study to enhance their knowledge of the nutrient contributions of each meal component and as inputs for planning menus that meet children's nutritional requirements.

• Applied Biological Chemistry
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• 제27권2호
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• pp.129-134
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• 1984

콩나물을 온도별로 길러 생장과정별 단백질조성을 7.5% polyacryl amide gel 전기영동법으로 조사하고 조제표품단백질의 그것으로 동정하였다. 원료대두(大豆)에서는 주(主) band 5개(a,b,c,d 및 p)와 미소 band 10개가 나타났고 4일자자엽(日字子葉)에서는 8개의 주(主) band와 (o band포함)와 11개 미소 band로 최고 band수(數)를 보였다. 배축(胚軸)에서는 자엽(子葉)에서 발견(發見)되는 band들이 모두 나타났으나 분리(分離)가 선명치 못하였다. 생육이 경과함에 따라 자엽(子葉)에서는 a band가 먼저, b,c,d band가 다음으로 감소해 갔으며 미소 band o+p는 6일(日)까지 증가하다 그후 감소하였다. 배축(胚軸)에서는 모든 band가 계속 증가하였는데 미소 band와 b+c+d가 증가속도가 컸다. a band는 7S, b+c+d는 11S, o+p는 2S globulin으로 동정되었다. 생육온도가 높아짐에 따라 자엽(子葉)에서의 감소와 배축(胚軸)에서의 증가속도가 빨라졌으며 7S보다 11S가 촉진되었다.

• BMB Reports
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• 제43권10호
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• pp.670-676
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• 2010

In this paper, a novel approach, ELM-PCA, is introduced for the first time to predict protein subcellular localization. Firstly, Protein Samples are represented by the pseudo amino acid composition (PseAAC). Secondly, the principal component analysis (PCA) is employed to extract essential features. Finally, the Elman Recurrent Neural Network (RNN) is used as a classifier to identify the protein sequences. The results demonstrate that the proposed approach is effective and practical.

• Applied Biological Chemistry
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• 제7권
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• pp.85-91
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• 1966

한국(韓國) 재배(栽培) 대두(大豆) 25품종(品種)을 선택하여 각(各) 품종(品種)의 단백질(蛋白質) 패턴을 여지전기영동법(濾紙電氣泳動法)으로 시행하여 품종간(品種間)의 차이(差異)를 비교(比較)해 보았다. 이 방법(方法)으로 5종(種)의 단백질(蛋白質)을 분리(分離)할 수 있었으며 각(各) 품종간(品種間)의 정량적(定量的)인 함율차(含率差)를 찾아 볼 수 있었다. 각(各) 단백질(蛋白質) 성분간(成分間)의 평균(平均) 함량비(含量比)는 다음과 같다. I 14.47% II 7.22% III 4.43% IV 71.70% V 2.18% 상기(上記) 각획분(各劃分)을 동정(同定)하여 본 결과(結果)는 다음과 같다.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2003년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.175-176
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• 2003

Dietary protein is the most expensive component in flounder feed because of the high protein requirement of this species. The non-protein energy sources may also influence the protein utilization of fish. The inclusion of adequate levels of non-protein energy sources in diets can minimize use of protein as an energy source. Protein sparing effect by fat and carbohydrate has been studied in other fish. (omitted)

• 생명과학회지
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• 제6권3호
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• pp.213-218
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• 1996

대장균의 GroEL과 상동성을 가지는 symbionin이 진딧물의 세포내 곤생미생물에서 유일하게 생산된다. 이것은 in vitro와 in vivo에서 moecular chaperone 활성을 가지는 것과 함께 자가인산화(autophosphory-lation)와 인산기전이효소(phosphotransferase)의 활성에 의해서 고에너지 인산기를 다른 곳에 줄 수 있다. Symbionin은 two component pathway의 센서분자의 역할을 하며, 지금까지 알려진 것과는 다른 성질을 가진 protein Kinase이다.