• Title/Summary/Keyword: complex separation

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Clomipramine treatment of acral lick dermatitis in a dog

  • Kang, Min-Hee;Lee, Chang-Min;Sung, Hyun-Jeong;Park, Hee-Myung
    • Korean Journal of Veterinary Research
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    • v.53 no.3
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    • pp.185-187
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    • 2013
  • An 11-year-old, toy poodle dog was presented with dermatologic lesions and mammary gland tumor (MGT) evaluation. A solitary, lobulated MGT (size $2.5{\times}3.5$ cm) was affecting the 5th left mammary gland. Firm, oval plaque skin lesions were present on the left dorsal carpal area. The skin lesions were alopecic and salivary staining. The dog had historical separation anxiety and excessive licking of skin lesions were observed. Based on the clinical and histopathologic examinations, MGT was diagnosed with mammary complex adenoma and the skin lesions were diagnosed with acral lick dermatitis. Behavior modification treatment using oral clomipramine was effective.

Adsorption Properties of Uranium on Acrylic Fibers Treated with Hydroxylamine (하이드록실 아민으로 처리한 아크릴 섬유의 우라늄 흡착특성)

  • Chin Young Gil;Lee Jung Sook
    • Journal of the Korean Society of Clothing and Textiles
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    • v.14 no.2
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    • pp.98-103
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    • 1990
  • Fibrous adsorbents containing amidoxime group which make chelate complexes with uranyl ions are studied for the recovery of uranium from sea water. Acrylic fibers are used as base Polymer. The adsorption properties of uranium are carried out to examine pH effect, concen-tration dependence, adsorption rate, separation, and chelate complex. The results obtained are as follows; 1. Metal capacity of U (VI) ion is in the range of pH $2\~10.2$. Amidoxime group-containing fiber recover U (VI) ions existed in sea water or waste water in extremely small quantities. 3. Using amidoxime group-containing fiber Cu (II) and U (VI) are separated with each other in dilute nitric acid solution (pH 2.3). 4. U (VI) chelate complexes are conformed by tridendate ligands, which are coordinated with one nitrogen and two oxygens onto amidoxime group-containing fiber.

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A Study on the Design of Interlaced Controller-Observer for Nonlinear Systems (비선형시스템의 관측기 제어기 조합 설계에 관한 연구)

  • Lee, Chun-Keun;Yang, Hai-Won
    • Proceedings of the KIEE Conference
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    • 1997.07b
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    • pp.682-684
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    • 1997
  • In the design of nonlinear control systems separation principle does not hold. Moreover even an exponentially decaying disturbances can destabilize a nonlinear system and lead to finite escape time from certain initial conditions. So we present a simple output feedback speed controller of induction motors which is designed to minimize the effects of observation error using the Kanellakopoulos et al's interlaced controller-observer design method. Simulation shows that the proposed controller has desired performance like another complex controllers.

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An Experimental Study on Wake Cavity Flow Characteristics of Two-dimensional Wedge Shaped Control Fins (2차원 쐐기형 제어핀 후류의 공동유동 특성에 대한 실험연구)

  • Jeong, So-Won;Ahn, Byoung-Kwon
    • Journal of the Society of Naval Architects of Korea
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    • v.53 no.3
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    • pp.180-187
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    • 2016
  • As distinct from a slender body, the separation of the boundary layer on a bluff body give rise to complex wakes in which various kinds of vortices form, develop and interact with each other. In this paper, we investigate cavitation wake field behind wedge shaped two-dimensional fin models. Eight different models are tested at the Chungnam National University Cavitation Tunnel (CNU-CT). First, we measure wake cavity shapes and compare with numerical results, which shows the good agreement with each other. In addition, we demonstrate that wake flow characteristics of the control fin are clearly identified by the correlation analysis of high-speed camera images and pressure fluctuation measurements.

Ultra-Specific Enrichment of GST-Tagged Protein by GSH-Modified Nanoparticles

  • Lee, Yeon-Ji;Park, Jong-Moon;Huh, Ji-Young;Kim, Min-Sik;Lee, Je-Sun;Palani, Arudra;Lee, Kwang-Yeol;Lee, Sang-Won
    • Bulletin of the Korean Chemical Society
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    • v.31 no.6
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    • pp.1568-1572
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    • 2010
  • The selective isolation of specific proteins from complex protein mixtures by nanoparticles is reported. Glutathionemodified superparamagnetic nanoparticles were used to purify specific proteins fused with glutathione S-transferase via enzyme-substrate interactions. They demonstrated greatly improved selectivity and efficiency over micron sized capturing beads. The ultra-specific enrichment of target proteins was confirmed by both SDS-PAGE and LC/MS/MS experiments.

Determination of ${\beta}$-Lactam Antibiotics by Gas-Chromatography with Flame Photometric Detector (II) (GC/FPD를 利用한 ${\beta}$-락탐系 抗生物質의 分析(II))

  • Park, Man-Ki;Cho, Yung-Hyun;Yang, Jeong-Seon;Park, Jeong-Hil
    • YAKHAK HOEJI
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    • v.28 no.4
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    • pp.243-248
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    • 1984
  • For gas chromatographic determination with the sulfur-specific flame photometric detector, nine ${\beta}$-lactam antibiotics without ${\alpha}$-amino group were esterified with borontrifluoride-methanol complex and then N-benzoylated with benzoyl chloride. The gas chromatographic separation of these products was successfully carried out on various silicon polymers (OV-1, OV-101, OV-17, OV-225, and QF-1) coated on the acid washed, silanized diatomite. The structure of the esterified and N-benzoylated product was confirmed by mass spectromer.

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Production of an Anticoagulant Hirudin by Fed-batch and Continuous Cell Recycle Fermentations Using Recombinant Saccharomyces cerevisiae (유가식과 세포재순환 연속공정을 이용한 항혈전제 hirudin의 생산)

  • 최치민;김명동;이상기;서진호
    • KSBB Journal
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    • v.13 no.4
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    • pp.456-460
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    • 1998
  • Fed-batch fermentations were carried out in order to improve the efficiency of hirudin production by recombinant Saccharomyces cerevisiae. A fed-batch fermentation done with the optimized semi-synthetic medium resulted in a maximum hirudin concentration of 342mg/$\ell$ by keeping a galactose concentrations between 10 and 30g/$\ell$ which corresponded to a 11.4-fold increase in hirudin concentration compared with the simple bach fermentation done with the same medium. Comparison of the chromatographic pattern of proteins in the growth medium clearly showed that the use of the semi-synthetic medium is more advantageous for separation of hirudin than the case o fusing the complex medium. Continuous cell recycle fermentation done at dilution rate of 0.1h-1 and an inlet galactose concentration of 100g/$\ell$ yielded a maximum hirudin productivity of 19.1mg hirudin/$\ell$$.$h.

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A study on the optimal HPLC condition for peptides complex analysis using mass spectrometry (질량분석기를 단백질 분석에 적용하기 위한 고성능액체크로마토그래피 최적조건 연구)

  • Kwon, Sung Won;Park, Chul Hong
    • Analytical Science and Technology
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    • v.16 no.1
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    • pp.78-81
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    • 2003
  • Peptides separation in high performance liquid chromatography (HPLC) is very important for the analysis of total proteins using mass spectrometry rather than two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). In this study, we investigated the optimal HPLC condition of peptides for the use of mass fingerprinting. As a result of pursuing a combination of solvent additives for HPLC, water and acetonitile containing both 0.1% trifluoroacetic acid and 0.1% acetic acid respectively showed the most efficient resolution and sensitivity.

Purification and Characterization of Crystalins by Aqueous Two-Phase Extraction

  • Bermudez, Ondrea;Forciniti, Daniel
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.6
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    • pp.395-401
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    • 2001
  • Crystallins are a family of water-soluble proteins that constitute up to 90% of the wa-ter-soluble proteins in mammalian eye lenses, We present in this paper an alternative purification method for these proteins using polyethylene glycol/dextran aqueous two-phase extraction. Un-der the appropriate conditions, we were able to recover the γ-crystallin fraction essentially free of the remaining proteins. High concentrations of salt at a neutral pH maximize the recovery of γ-crystallins in the top phase and minimize the contamination by the other proteins present in the lenses. The proposed protocol decreases the separation time by about 50%. The complex partition behavior observed for these proteins reflects a delicate balance between protein/phase-forming species(various polymers and salts) and protein interactions. This is evidenced, in part, by the role played by the largest proteins in this group as a "pseudo"phase-forming species.

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Vibration Characteristics of the Floor Structures inserted with Damping Materials (제진재가 삽입된 바닥 구조의 진동특성)

  • Jeong, Young;Yoo, Seung-Yub;Jeon, Jin-Yong;Park, Jun-Hong
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2005.11a
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    • pp.377-380
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    • 2005
  • Damping materials encompass a broad range of materials, including, but not limits to, pressure sensitive adhesives, epoxies, rubbers, foams, thermoplastics, enamels and mastics. Their common characteristic is that their modulus is represented by a complex quantity, possessing both a stored and dissipative energy component. Loss factor of damping material analyzed more than 2 times than rubber to 1.5 $\sim$ 2.3, could know that Damping layer has excellent attenuation performance in side of vibration reduction. Measurements of vibration using accelerometers by adhesion of Damping layer, square Plate by Separation of Damping layer is less binding of Damping layer, analyzed low loss factor and Natural Frequency by free Vibration of Square Plate.

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