Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Ultra-Specific Enrichment of GST-Tagged Protein by GSH-Modified Nanoparticles

  • Received : 2010.02.23
  • Accepted : 2010.03.30
  • Published : 2010.06.20
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Abstract

The selective isolation of specific proteins from complex protein mixtures by nanoparticles is reported. Glutathionemodified superparamagnetic nanoparticles were used to purify specific proteins fused with glutathione S-transferase via enzyme-substrate interactions. They demonstrated greatly improved selectivity and efficiency over micron sized capturing beads. The ultra-specific enrichment of target proteins was confirmed by both SDS-PAGE and LC/MS/MS experiments.

Keywords

References

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