• BMB Reports
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• v.35 no.2
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• pp.206-212
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• 2002

The NS2B-NS3(pro) polyprotein segment from the dengue virus serotype 2 strain 16681 was purified from overexpressing E. coli by metal chelate affinity chromatography and gel filtration. Enzymatic activity of the refolded NS2B-NS3(pro) protease complex was determined in vitro with dansyl-labeled peptide substrates, based upon native dengue virus type 2 cleavage sites. The 12mer substrate peptides and the cleavage products could be separated by reversed-phase HPLC, and were identified by UV and fluorescence detection. All of the peptide substrates (representing the DEN polyprotein junction sequences at the NS2A/NS2B, NS2B/NS3, NS3/NS4A and NS4B/NS5 sites) were cleaved by the recombinant protease NS2B-NS3(pro). No cleavage was observed with an enzymatically inactive S135A mutant of the NS3 protein, or with a modified substrate peptide of the NS3/NS4A polyprotein site that contained a K2093A substitution. Enzymatic activity was dependent on the salt concentration. A 50% decrease of activity was observed in the presence of 0.1M sodium chloride. Our results show that the NS3 protease activity of the refolded NS2B-NS3(pro) protein can be assayed in vitro with high specificity by using cleavage-junction derived peptide substrates.

• Journal of the Korean Geotechnical Society
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• v.19 no.3
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• pp.93-104
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• 2003

The Disturbed State Concept (DSC) model, with simplified unloading/reloading formulation, is implemented in a nonlinear dynamic finite element program fur porous media named DSC_DYN2D. In this research, the DSC constitutive model is utilized using the HiSS model for relative intact (RI) part and the critical state model for the fully adjusted (FA) part in the material. The general formulation for implementation is developed. The cyclic loading tests from the field load test data on a pile segment were numerically simulated using the finite element program DSC_DYN2D and compared with field measurements and those from the previous analysis with the HiSS model. The DSC predictions show improved agreement with the field behavior of the pile compared to those from the HiSS model. Overall, the computer procedure with the DSC model allows improved and realistic simulation of the complex dynamic soil-structure interaction problems.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.8A
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• pp.805-813
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• 2002

Document image should be classified into detailed regions as text, picture, table and etc through the geometric layout analysis if paper documents can be converted automatically into electronic documents. However, complexity of the document layout and variety of the size and density of a picture are the reason to make it difficult to analyze the geometric layout of the document images. In this paper, we propose the method which have a better performance of the region segmentation and classifications, and the line extraction in the table region than the commercial softwares and previous methods. The proposed method can segment the document into detailed regions by using connected components method even if its layout is complex. This method also classifies texts and pictures by using separable median filter even. Though their size and density are diverse, In addition, this method extracts the lines from the table adapting one dimensional median filter to the each horizontal and vertical direction, even though lines are deformed or texts attached to them.

• Journal of Chest Surgery
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• v.28 no.7
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• pp.658-665
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• 1995

Between March 1989 and December 1994, one-stage repair was performed for correction of the intracardiac malformations associated with aortic coarctation in 34 patients or interrupted aortic arch in 8 patients via median sternotomy. There were 26 male and 16 female patients, and their body weight ranged from 1.8 to 8kg[mean weight, 4.0$\pm$l.4kg . The age at the operation ranged from 7 days to 18 months [mean age, 3.1$\pm$3.8 months . The repair of aortic coarctation or interrupted aortic arch was performed using extended end-to-end anastomosis in most of the patients[86%, 36/42 , and six patients underwent ductal tissue excision and patch aortoplasty. Intracardiac defects were corrected concomitantly through the right atrium unless the anatomy dictated otherwise. Obstructive outlet septurn was resected whenever necessary. There were seven early deaths[16.8% , and three late deaths with a mean follow-up period of 25 months [range from 1 to 65 months . Three patients were reoperated upon residual subaortic stenosis,stenosis at the RPA origin, and subacute bacterial endocarditis respectively. None showed any significant residual or anastomotic stenosis postoperatively. One stage repair of the aortic coarctation and interrupted aortic arch associated with intracardiac defect leaves no native coarctation shelf tissue or residual hypoplasia in the repaired segment, has low incidence of recurrent or residual stenosis, minimizes reoperation and incisions, and manages arch hypoplasia easily.We conclude that surgical results of one-stage repair for the intracardiac malformation associated with aortic coarctation or interrupted aortic arch are resonable.

• The korean journal of orthodontics
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• v.51 no.5
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• pp.321-328
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• 2021

Objective: To examine the accuracy of computer-aided intraoperative navigation (Ci-Navi) in bimaxillary orthognathic surgery by comparing preoperative planning and postoperative outcome. Methods: The study comprised 45 patients with congenital dentomaxillofacial deformities who were scheduled to undergo bimaxillary orthognathic surgery. Virtual bimaxillary orthognathic surgery was simulated using Mimics software. Intraoperatively, a Le Fort I osteotomy of the maxilla was performed using osteotomy guide plates. After the Le Fort I osteotomy and bilateral sagittal split ramus osteotomy of the mandible, the mobilized maxilla and the distal mandibular segment were fixed using an occlusal splint, forming the maxillomandibular complex (MMC). Real-time Ci-Navi was used to lead the MMC in the designated direction. Osteoplasty of the inferior border of the mandible was performed using Ci-Navi when facial symmetry and skeletal harmony were of concern. Linear and angular distinctions between preoperative planning and postoperative outcomes were calculated. Results: The mean linear difference was 0.79 mm (maxilla: 0.62 mm, mandible: 0.88 mm) and the overall mean angular difference was 1.20°. The observed difference in the upper incisor point to the Frankfort horizontal plane, midfacial sagittal plane, and coronal plane was < 1 mm in 40 cases. Conclusions: This study demonstrates the role of Ci-Navi in the accurate positioning of bone segments during bimaxillary orthognathic surgery. Ci-Navi was found to be a reliable method for the accurate transfer of the surgical plan during an operation.

• Research in Community and Public Health Nursing
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• v.31 no.spc
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• pp.525-533
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• 2020

Purpose: Workers in special employment relationship (WSERs) are workers in nonstandard employment arrangements who lack worker protection accorded in standard employment arrangements. This study aimed to describe self-rated health (SRH) and depressive symptoms (DS) among Korean WSERs in comparison to regular wage workers (RWW) and identify associations between working conditions and those outcomes. Methods: In this study, secondary data analysis using the 5th Korean Working Conditions Survey was used. The sample totaled 29,120, including 1,538 WSERs and 27,564 RWWs. Sociodemographic and work-related characteristics were employed as explanatory variables and SRH and DS as dependent variables. Using multiple logistic regression, the determinants of fair/poor SRH and DS were identified. Results: The prevalence rates for fair/poor SRH and DS in WSERs were 25.2% and 28.3%, respectively, and 20.7% and 25.0% in RWWs, respectively. Compared to RWWs, WSERs had 31% (aOR=1.31, 95% CI=1.14~1.49) and 20% (aOR=1.20, 95% CI=1.06~1.36) higher odds of SRH and DS, respectively. Some factors, such as a lack of rest guarantee and sickness presenteeism, had a larger influence in the WSER than in the RWW group. Conclusion: Compared to RWWs, WSERs reported having poorer working conditions and were more likely to report poor general and mental health. Therefore, in Korea, public health policymakers should consider measures to protect the working conditions and health of WSERs, a growing segment of the working population. The study produced new epidemiological evidence regarding the relationships between employment arrangements and health.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.24 no.1
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• pp.50-56
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• 2020

With the rapid development of deep neural network theory and application research, the effect of face detection has been improved. However, due to the complexity of deep neural network calculation and the high complexity of the detection environment, how to detect face quickly and accurately becomes the main problem. This paper is based on the relatively simple model of the MTCNN model, using FDDB (Face Detection Dataset and Benchmark Homepage), LFW (Field Label Face) and FaceScrub public datasets as training samples. At the same time of sorting out and introducing MTCNN(Multi-Task Cascaded Convolutional Neural Network) model, it explores how to improve training speed and Increase performance at the same time. In this paper, the dynamic image pyramid technology is used to replace the traditional image pyramid technology to segment samples, and OHEM (the online hard example mine) function in MTCNN model is deleted in training, so as to improve the training speed.

• Journal of Biomedical Engineering Research
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• v.42 no.3
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• pp.100-106
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• 2021

Measuring Intima-media thickness (IMT) with ultrasound images can help early detection of coronary artery disease. As a result, numerous machine learning studies have been conducted to measure IMT. However, most of these studies require several steps of pre-treatment to extract the boundary, and some require manual intervention, so they are not suitable for on-site treatment in urgent situations. in this paper, we propose to use deep learning networks U-Net, Attention U-Net, and Pretrained U-Net to automatically segment the intima-media complex. This study also applied the HE, HS, and CLAHE preprocessing technique to wireless portable ultrasound diagnostic device images. As a result, The average dice coefficient of HE applied Models is 71% and CLAHE applied Models is 70%, while the HS applied Models have improved as 72% dice coefficient. Among them, Pretrained U-Net showed the highest performance with an average of 74%. When comparing this with the mean value of IMT measured by Conventional wired ultrasound equipment, the highest correlation coefficient value was shown in the HS applied pretrained U-Net.

• Journal of the Korea Fashion and Costume Design Association
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• v.24 no.3
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• pp.33-48
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• 2022

This study aimed to investigate bag purchase behaviors according to materialism value. The subjects were 443 male and female adult consumers in their 20s to 50s. The research method was a survey, and the questionnaire consisted of questions on materialism value, bag purchase behaviors, and demographic characteristics. For data analysis, descriptive statistics, Cronbach's α, χ² test, factor analysis, cluster analysis, ANOVA, and Duncan's multiple range test were performed. The results of this study were as follows. First, materialism value was derived from three factors (happiness pursuit, possession-oriented, and success judgment). Second, subjects could be divided into three groups (happiness pursuit group, success judgment pursuit group, and immaterialism group) based on the materialism value variable. Third, the derived groups showed many differences in bag purchase behaviors. The happiness pursuit group considered all bag evaluation criteria factors (practicality, aesthetics, economy, symbolism) and bag purchasing information sources factors (mass media and personal sources) more than other groups, and showed a tendency to prefer select shops and complex shopping malls as bag purchasing places. In addition, the average annual cost and frequency of purchasing bags of this group were higher than those of other groups. The success judgment pursuit group considered symbolism as a bag evaluation criteria more than other groups, and considered personal sources as bag purchasing information sources more than mass media sources, and preferred luxury stores and department stores as bag purchasing places. On the other hand, the immaterialism group considered practicality and aesthetics as bag evaluation criteria and placed less importance on all information sources than other groups, and preferred Internet shopping malls as purchasing places. This group had the lowest average annual purchase cost and frequency among the three consumer groups. This study suggested that materialism value is a useful variable to segment male and female adult consumer markets effectively, and to understand the bag purchase behaviors of consumer groups divided by materialism value.

• Geomechanics and Engineering
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• v.28 no.5
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• pp.531-546
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• 2022

Ground displacements caused by the construction of overlapping twin shield tunnels with small turning radius are complex, especially under special geological conditions of construction. To investigate the ground displacements caused due to shield machines in the unique calcareous sand layers in Israel for the first time and determine the main factors affecting the ground displacements, field monitoring, laboratory geological analysis, theoretical calculations, and parameter studies were adopted. By using rod extensometers, inclinometers, total stations, and automatic segment-displacement monitors, subsurface tunneling-induced displacement, surface settlement, and displacement of the down-track tunnel segments caused by the construction of an up-track tunnel were analyzed. The up-track tunnel and the down-track tunnel pass through different stratum, resulting in different construction parameters and ground displacements. The laws of variation of thrust and torque, soil pressure in the chamber, excavated soil quantity, synchronous grouting pressure, and grout volume of the two tunnels from parallel to fully overlapping orientations were compared. The thrust and torque of the shield in the fine sand are larger than those in the Kurkar layer, and the grouting amount in fine sand is unstable. According to fuzzy statistics and Gaussian curve fitting of the shield tunneling speed, the tunneling speed in the Kurkar stratum is twice that in the fine-sand stratum.