• Journal of Microbiology and Biotechnology
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• 제20권8호
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• pp.1230-1239
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• 2010

Five bacterial species, capable of degrading the recalcitrant organic compounds (ROCs) diethyleneglycol monomethylether (DGMME), 1-amino-2-propanol (APOL), 1-methyl-2-pyrrolidinone (NMP), diethyleneglycol monoethylether (DGMEE), tetraethyleneglycol (TEG), and tetrahydrothiophene 1,1-dioxide (sulfolane), were isolated from an enrichment culture. Cupriavidus sp. catabolized $93.5{\pm}1.7$ mg/l of TEG, $99.3{\pm}1.2$ mg/l of DGMME, $96.1{\pm}1.6$ mg/l of APOL, and $99.5{\pm}0.5$ mg/l of NMP in 3 days. Acineobacter sp. catabolized 100 mg/l of DGMME, $99.9{\pm}0.1$ mg/l of NMP, and 100 mg/l of DGMEE in 3 days. Pseudomonas sp.3 catabolized $95.7{\pm}1.2$ mg/l of APOL and $99.8{\pm}0.3$ mg/l of NMP. Paracoccus sp. catabolized $98.3{\pm}0.6$ mg/l of DGMME and $98.3{\pm}1.0$ mg/l of DGMEE in 3 days. A maximum $43{\pm}2.0$ mg/l of sulfolane was catabolized by Paracoccus sp. in 3 days. When a mixed culture composed of the five bacterial species was applied to real wastewater containing DGMME, APOL, NMP, DGMEE, or TEG, 92~99% of each individual ROC was catabolized within 3 days. However, at least 9 days were required for the complete mineralization of sulfolane. Bacterial community diversity, analyzed on the basis of the TGGE pattern of 16S rDNA extracted from viable cells, was found to be significantly reduced in a conventional bioreactor after 6 days of incubation. However, biodiversity was maintained after 12 days of incubation in an electrochemical bioreactor. In conclusion, the electrochemical reduction reaction enhanced the diversity of the bacterial community and actively catabolized sulfolane.

• 환경생물
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• 제28권3호
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• pp.143-149
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• 2010

2007년 3월부터 2009년 1월까지 울산 태화강에 서식 하는 어류의 군집 구조 및 병원체에 대하여 조사하였다. 그 결과 태화강의 3개 조사지점으로부터 채집된 어류는 모두 총 9목 17과 35종이었으며 개체수로서의 우점종은 끄리로서 39%였으며 다음으로 누치가 30.9%였다. 한국고유종은 전체의 20.8%로서 참몰개, 참갈겨니, 기름종개, 꺽지, 동사리의 5종이었다. 누치와 끄리와 같은 대형 어류가 조사지점 2인 삼호교 인근 수역에 계절에 따라 모여들었다. 220개체의 어류에 대한 2종의 어류 병원성 바이러스에 대한 반응은 모두 음성으로 나타났으며, 어류에 병원성이 있다고 알려진 세균은 분리되지 않았다. 어류 병원성 기생충은 총 7종으로 다양하게 나타나지는 않았다. 특히 트리코디나충은 매월 검출이 되었으며 그 감염 밀도도 비교적 높은 편이었다. 그러나 하천의 일정지점에서의 일시적인 어류 과밀현상은 질병에 의한 대량폐사에 직접적인 영향을 주지는 않을 것으로 생각된다.

• Journal of Microbiology and Biotechnology
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• 제15권2호
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• pp.376-383
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• 2005

Alcaligenes sp. JMP228 carrying 2,4­dichlorophenoxyacetic acid (2,4-D) degradative plasmid pJP4 was inoculated into natural soil, and transfer of the plasmid pJP4 to indigenous soil bacteria was investigated with and without 2,4-D amendment. Plasmid pJP4 transfer was enhanced in the soils treated with 2,4-D, compared to the soils not amended with 2,4-D. Several different transconjugants were isolated from the soils treated with 2,4-D, while no indigenous transconjugants were obtained from the unamended soils. Inoculation of the soils with both the donor Alcaligenes sp. JMP228/pJP4 and a recipient Burkholderia cepacia DBO 1 produced less diverse transconjugants than the soils inoculated with the donor alone. Repetitive extragenic palindromic-polymerase chain reaction (REP-PCR) analysis of the transconjugants exhibited seven distinct genomic DNA fingerprints. Analysis of 16S rDNA sequences indicated that the transconjugants were related to members of the genera Burkholderia and Pandoraea. Denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA genes revealed that inoculation of the donor caused clear changes in the bacterial community structure of the 2,4-D­amended soils. The new 16S rRNA gene bands in the DGGE profile corresponded with the 16S rRNA genes of 2,4-D­degrading transconjugants isolated from the soil. The results indicate that introduction of the 2,4-D degradative plasmid as Alcaligenes sp. JMP228/pJP4 has a substantial impact on the bacterial community structure in the 2,4-D-amended soil.

• 대한환경공학회지
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• 제35권8호
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• pp.571-578
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• 2013

본 연구에서는 음폐수를 이용 낮은 유기물 부하율에서 소화조 내부 pH 조절 유무에 따른 소화조 운전의 바이오가스 발생량 및 미생물 군집도 변화에 대한 비교 분석했다. 그 결과, 내부 pH를 조절하지 않은 반응조는 pH, Free ammonia, Volatile fatty acid의 증가에 의한 반응조 안정성이 떨어짐에도 불구하고 내부 pH 조절 반응조와 비슷한 바이오가스 전환율을 보였다. 이는 미생물 군집도 분석 결과에 따르면 외부환경에 대한 내성이 강한 Methanosarcina sp.의 우점에 의해 반응조의 안정성을 유지할 수 있었던 것으로 나타났다.

• 미술이론과 현장
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• 제3호
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• pp.35-50
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• 2005

For us, any regional art exist? Almost all art events today take place mostly in Seoul. Concentrating on the reinforcement of its power, the regional art world remains isolated without any connection with the Seoul art community. It is completely closed off from the central art scene. The regional art world seems to consider that more helpful in consolidating its status. It is in real sense suffering the absence of art criticism and art media, lack of an understanding of art and backwardness of art education. Many regional artists are dreaming of moving out from their domain, aspiring to be a member of the central art world. They make an effort to assimilate into a refined, modern style of the central art circle, rather than striving to create works imbued with regionality and locality. As the artists living in the provinces, the characteristics of their district should be above all else reflected in their art. Although they are less informed about the latest trend of culture and art, their art has to be something passionately and obstinately embodying the intrinsic quality of their regions. They have to find a practical solution more positively, instead of merely complaining about the poor condition of the regional art community. There is the need to bring about a turnabout in our awareness that regional art is confined to any limit and cannot be in the center of the entire art world. What' s most significant is to dissolve factionalism and not to depend on school ties or regional networks. As a reaction against authoritarianism, regional art has to put emphasis on attaining its originality. For this, regional art should respect regional tradition, history and background and break away from an old convention of wielding power.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제25권1호
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• pp.41-51
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• 2022

Purpose: Community-acquired bacterial enteritis (CABE) is a common problem in developed countries. It is important to understand the epidemiologic changes in bacterial pathogens for prevention and treatment. Therefore, we studied the epidemiologic changes in CABE in Korean children. Methods: A total of 197 hospitalized pediatric patients aged <19 years that presented with dysentery symptoms and showed positive polymerase chain reaction results for bacterial species in stool samples, were enrolled in this study for 10 years (June 2010 to June 2020). We classified patients in phase I (06, 2010-06, 2015) and phase II (07, 2015-06, 2020) and analyzed their epidemiologic and clinical characteristics. Results: The most common pathogens were Campylobacter species (42.6%) and Salmonella species were the second most common pathogens (23.9%). The abundance of pathogens decreased in the following order: Clostridium difficile (9.6%), Shigella (5.6%), and Clostridium perfringens (5.6%). Escherichia coli O157:H7 was found to be the rarest pathogen (2.0%). Campylobacter species showed an increase in the infection rate from 32.1% in phase I to 49.6% in phase II (p=0.0011). Shigella species showed a decline in the infection rate in phase I from 14.1% to 0.0% in phase II (p<0.001). C. difficile and C. perfringens showed an increase in infection rate in phase II compared to phase I, but the difference was not statistically significant. Conclusion: The infection rate of Campylobacter species in CABE has been rising more recently, reaching almost 50%. This study may help establish policies for prevention and treatment of CABE in Korean children.

• 한국제4기학회지
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• 제17권2호
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• pp.85-99
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• 2003

Since the experience of major landscape change during last half of the century due to war and rapid urbanization, the traditional character has been weakened, and it is necessary for a reconsideration to improve the landscape for the future. To review these relationships, the importance of a comprehensive understanding of nature has been suggested. Therefore identification of a new concept based on the 'socio-cultural influence of landscape' and 'sense of place' which are related to peoples' previous experience, is required. Furthermore more practical definitions and criteria to reveal the relationship are necessary. Among the terms suggested to describe sense of place such as 'home', 'place identity', 'place-based meaning' and 'settlement identity' etc., the 'home' is selected to represent our surrounding landscape. For more practical classification of home landscape, additional terms are suggested and defined based on both the relationships between human beings and nature, and between memory derived from previous experience and shared values with in the community. The additional terms which are the most important in the role of landscape character related to humans' are; ⅰ) Personal Landscape: Landscape of an individual human, which derives from previous personal experience; involves distinguishable character for a given person, and it is emotional and flexible depending on circumstances. ⅱ) Ordinary Landscape： Landscape of the 'common interest' between members of a community, which is acceptable as a surrounding for everyday daily life, it produces the richness and variety of landscape. ⅲ) Kernel Landscape： Landscape of the 'common ground' which is acceptable to the majority members of the community, and it provides variety and stability for periods of time, and it could strongly represent community attitudes toward nature. ⅳ) Prototype Landscape: Landscape as the 'common denominator' of overall community from past to present and towards the future, which encompasses all the kernel landscape throughout history. It provides a sense of place, balances the homogeneity of character throughout overall communities. Some part of this can be shared throughout history to shape an overall sense of place. It can also represent short terms fashions. For a prototype landscape to reveal sense of place, there are a couple of points which we should underline the commencing point. Firstly, understanding the relationship between humans and nature should be based on a given character of surroundings. Secondly, reoccurring landscape elements which have sustained in history can lead to sense of place, and should be reviewed the influences between nature and humans.

• 농촌의학ㆍ지역보건
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• 제40권4호
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• pp.221-227
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• 2015

본 연구는 관절염 환자들의 신체활동에 대한 제한, 이러한 활동에 대한 상대적 의존성 그리고 우울감 사이의 관련성을 매개효과를 밝히고자 연구를 실시하였다. 연구대상자는 지역사회에 거주하는 여성 농업인 중 관절염을 앓고 있다고 응답한 자 중 209명을 임의로 추출하여 설문조사 방식으로 조사하였다. 결과적으로 관절염으로 인한 세 개의 활동영역(개인의 신변활동, 집안일, 거주지에서의 이동성 등)은 활동제한이 우울감에 직접 관련이 있다기 보다는 활동제한으로 인해 의존성이 발생되고 결과적으로 우울감이 발생되는 경로를 보여 의존성을 개선하였을 경우 우울감이 줄어들거나 극복될 수 있으며, 여가생활에 대한 활동제한은 우울감에 직접적인 효과가 있어 관절염환자들의 활동제한 중에서 무엇보다도 여가생활의 제한은 우울감에 직접적인 영향을 주므로 환자들에게서 여가생활을 적극 장려할 수 있는 프로그램 개발이 필요하다. 결론적으로 관절염환자들의 자기관리능력 함양, 보조 기구 지급을 통한 의존성의 개선은 이들 환자들의 우울감 개선이 될 것이다.

• 자원환경지질
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• 제53권6호
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• pp.655-666
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• 2020

본 연구는 함안지역 고농도 비소 오염 지하수의 미생물 군집 특성 분석을 통해 지하수 내 비소 산출 특성을 평가하고자 수행되었다. 함안지역 지하수는 0~757.2 ㎍/L 농도 범위의 비소로 오염되어 있으며, 이는 국내에서 보고된 가장 높은 자연기원 지하수 비소 오염 농도를 나타낸다. 전체 200개 시료 중 29개(14.5%)에서 먹는물 수질 기준인 10 ㎍/L 이상의 비소 농도가 관측되었으며, 암반 지하수 관정(80~100 m 심도)에서는 50 ㎍/L 이상인 시료가 8개(4%) 존재하는 것으로 나타났다. 또한, 함안군 북측 지역에는 비소 함량이 100 ㎍/L 이상인 관정이 7개 존재하는 특징을 보였다. 고농도 비소 오염지역 지하수의 미생물 군집 분석 결과, 8개 시료에서 서로 상이한 군집 특성을 보였으며 Proteobacteria가 평균 61.5%로 가장 우점하였다. 속(genus) 수준에서는 혐기성 균인 Gallionella속과 Methermicoccus 속, 호기성 비소 환원균인 Acinetobacter속이 각각 12.8%, 7.3%, 7.8%의 높은 비율로 존재하고 있었다. 결과적으로 연구 지역의 지화학적 특성과 지하수 내 미생물의 생지화학적 기작의 복합적 반응에 의해 고농도 비소 오염 특성을 나타내는 것으로 판단된다. 따라서 본 연구를 통해 구축된 지화학적 특성과 미생물 군집에 대한 정보는 지하수 내 고농도 비소의 생성 기원과 이들의 제어 기술 개발에 활용이 가능할 것으로 여겨진다.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2002년도 추계학술대회논문집
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• pp.559-564
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• 2002

Recently a number of social surveys on community response to environmental noises have been conducted. Since standardized noise annoyance scales were needed to compare the results from various areas. ICBEN(International Commission on Biological of Noise) Team 6 planned a international joint study and constructed comparable standardized noise annoyance scales using the same method. In Korea the survey was conducted in four areas such as Seoul, Taejon, Taegu and Kwangju. About 100 subjects participated in each area approximately. The 21 adverbs were first in the carly survey, and five verbal annoyance modifiers were finally constructed as follows; 1(Jeonhyo), 2(Jokm), 3(Bikyojerk)m, 4(Ajoo), 5(Umcheongnage)